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Gene information
General information
TC013995 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3726090..3732684
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013995
    iBeetle screen iB_05538

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013995 RA
      MLQFLSFSR KTLSNNLSI YIKTNTGNT LSVDLDPTW 
      DIKEVKEFV APQLGLKPG EVKIIFAGK ELGDNISIS 
      ECDLGQQST LHAIKITKK PNPPTLNNA VIEESDEGK 
      PLCETLSDL SFSNDDSEE GPTEQRKVH FFVYCPTCK 
      ALKNGKLRV RCHFCKSGA FTVHSDPQN WNDVLQSKQ 
      ITGLCENNP ELCANVLNN KEPTFAEFY FKCSEHTSL 
      GETDEAVPL YLIRPNLKE VPCLACADI NETVFVFPC 
      KEGHVTCLD CFRQYCTTR LRERQFWQH PEFGYTLAC 
      PAGCPDSFI QEIHHFRLL SESQYTQYQ RFATEEYVL 
      RSGGVLCPQ PGCGMGILA DPECTKITC INGCGYVFC 
      RLCLQGFHI GECEAPEGD VGAPGDCSY NVDPTRAAE 
      ARWDEASKV AIKVLTKPC PKCRTPTER DGYKRLGNG 
      WKSLNEDER KKYFDRAKA IGLQHKKEF PVGENPTGL 
      QKITIDSFE LFPSTEIFS KRRKIYSL
    CDS sequence
    • >TC013995 RA
      atgttacaa tttctgtca tttagtaga aaaacgctg 
      agcaataat ttgagtatt tacataaaa accaacaca 
      gggaatacg ctttcggtc gatttggac cccacctgg 
      gacataaaa gaggtgaaa gagttcgtt gcgccccaa 
      ttgggactg aaaccaggc gaggtaaag attattttc 
      gctggaaaa gaactggga gacaacatt agtatttct 
      gaatgtgat ttgggccaa caaagcacc ctccacgct 
      atcaaaatc accaaaaag cccaaccct cccacttta 
      aacaatgcc gtaattgag gaaagtgac gagggcaaa 
      cctctgtgt gagacctta tcagatttg agtttttca 
      aacgatgat tcagaggaa gggcccacc gagcaacgc 
      aaagtccac ttttttgtc tattgcccc acgtgtaaa 
      gccctcaaa aatgggaaa ttgcgagtt cggtgccac 
      ttttgcaaa tcgggggct ttcacggtg cactcagac 
      ccccaaaac tggaatgat gtgctgcag tcaaagcag 
      ataaccggc ctttgtgag aataacccc gaattgtgt 
      gccaacgtt ctgaacaac aaagaaccg actttcgcc 
      gaattttat ttcaaatgc tcggagcac acgtccctc 
      ggagagacc gacgaggct gttcccctc tatctaatt 
      cgtccgaat ttaaaagaa gtgccttgt ctagcgtgc 
      gctgacata aacgaaacc gtgttcgtc tttccatgt 
      aaagaaggc cacgttact tgcctcgac tgtttccgc 
      cagtactgc acgacacgg ctgcgggaa cgccaattc 
      tggcaacac cccgagttt ggctacacg ctagcgtgc 
      cccgctggc tgtcccgac tccttcatc caggagatc 
      caccatttt cgcctcctg tcagagagt caatacaca 
      caatatcag agatttgcg actgaagag tatgtgttg 
      cgctccggt ggggtgctc tgcccccag ccgggctgc 
      ggcatgggc atactggcg gaccccgag tgcaccaaa 
      atcacgtgc atcaacggc tgcggctac gttttctgc 
      cgcctgtgc ctccagggg ttccacatt ggggagtgc 
      gaagcgccc gaaggggac gtgggggcc ccgggggat 
      tgttcgtac aatgtggac cccacaaga gccgcggaa 
      gcccgttgg gacgaagct tcgaaagtt gcgattaaa 
      gtcttgaca aagccctgt cccaaatgt cgcaccccc 
      actgaacga gatggttac aaacgcctg ggaaacggc 
      tggaagtcc ctgaacgag gacgagcgg aaaaaatat 
      ttcgatcgg gccaaagct atcggattg cagcacaaa 
      aaagaattc ccagtcgga gagaatcca accgggctt 
      caaaaaatt accatagac tcatttgaa ttgttcccc 
      tccacggaa atctttagt aaaaggcga aagatttat 
      tcgctttga
    mRNA sequence
    • >TC013995 RA
      ttttttcta aaaataagc ccggccgcg gttaccaca 
      gatgatgag ttttattat caacgtaat taaagattt 
      gttgcataa gatgttaca atttctgtc atttagtag 
      aaaaacgct gagcaataa tttgagtat ttacataaa 
      aaccaacac agggaatac gctttcggt cgatttgga 
      ccccacctg ggacataaa agaggtgaa agagttcgt 
      tgcgcccca attgggact gaaaccagg cgaggtaaa 
      gattatttt cgctggaaa agaactggg agacaacat 
      tagtatttc tgaatgtga tttgggcca acaaagcac 
      cctccacgc tatcaaaat caccaaaaa gcccaaccc 
      tcccacttt aaacaatgc cgtaattga ggaaagtga 
      cgagggcaa acctctgtg tgagacctt atcagattt 
      gagtttttc aaacgatga ttcagagga agggcccac 
      cgagcaacg caaagtcca cttttttgt ctattgccc 
      cacgtgtaa agccctcaa aaatgggaa attgcgagt 
      tcggtgcca cttttgcaa atcgggggc tttcacggt 
      gcactcaga cccccaaaa ctggaatga tgtgctgca 
      gtcaaagca gataaccgg cctttgtga gaataaccc 
      cgaattgtg tgccaacgt tctgaacaa caaagaacc 
      gactttcgc cgaatttta tttcaaatg ctcggagca 
      cacgtccct cggagagac cgacgaggc tgttcccct 
      ctatctaat tcgtccgaa tttaaaaga agtgccttg 
      tctagcgtg cgctgacat aaacgaaac cgtgttcgt 
      ctttccatg taaagaagg ccacgttac ttgcctcga 
      ctgtttccg ccagtactg cacgacacg gctgcggga 
      acgccaatt ctggcaaca ccccgagtt tggctacac 
      gctagcgtg ccccgctgg ctgtcccga ctccttcat 
      ccaggagat ccaccattt tcgcctcct gtcagagag 
      tcaatacac acaatatca gagatttgc gactgaaga 
      gtatgtgtt gcgctccgg tggggtgct ctgccccca 
      gccgggctg cggcatggg catactggc ggaccccga 
      gtgcaccaa aatcacgtg catcaacgg ctgcggcta 
      cgttttctg ccgcctgtg cctccaggg gttccacat 
      tggggagtg cgaagcgcc cgaagggga cgtgggggc 
      cccggggga ttgttcgta caatgtgga ccccacaag 
      agccgcgga agcccgttg ggacgaagc ttcgaaagt 
      tgcgattaa agtcttgac aaagccctg tcccaaatg 
      tcgcacccc cactgaacg agatggtta caaacgcct 
      gggaaacgg ctggaagtc cctgaacga ggacgagcg 
      gaaaaaata tttcgatcg ggccaaagc tatcggatt 
      gcagcacaa aaaagaatt cccagtcgg agagaatcc 
      aaccgggct tcaaaaaat taccataga ctcatttga 
      attgttccc ctccacgga aatctttag taaaaggcg 
      aaagattta ttcgctttg aagggaaac cagagtgac 
      gaaaaccac tctcagtag gaatttatc acgagaggg 
      acgtcccaa acgagcgaa acgctacaa gtttcgccc 
      tctattgga acacttcct actacataa cagctgagg 
      aaaacacaa agttgcaaa gtaaacatt aaactagta 
      aaagaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY