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Gene information
General information
TC013982 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3491451..3504315
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013982
    iBeetle screen iB_05536

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013982 RA
      MENVFYKTT IILYNTTSK NTTESSDSD TVNNWWALS 
      ALILAVGTA AGNILVCLA ICWERRLQN VTNYFLMSL 
      AITDLMVAI LVMPLGILT LIRGYFPLP SIYCLAWIC 
      LDVLFCTAS IMHLCTISV DRYLSLSYP MKFGRNKSR 
      RRVILKIVF VWLLSIAMS LPLSLMYSK DQGTLIKDG 
      TCQIPDPLY KLIGSIICF YIPLLVMLV TYALTVRLL 
      AHQRQNLGT PDWSSNWMG GPSALERRC TWRKLLTKA 
      KQTTPQHGH HSAASTDTE LTTIDNHEL WIQESEPTP 
      CTMSALGQF GAEMLKLSR GLEAASHLG TPPPRPSPG 
      KTILKISSG DSLADSHSS LLTPETSPW TSKRRRAST 
      FHEGDTRDE SPSPRWRKR GSSFHEPRS SDVSSELSS 
      EATESSTFP LPPPCTCPY FGEKQADKS PPPKPAEVK 
      IIPATKLAL EVEKSPLKK SLRNRSSDS SPNNIVVTW 
      EASRRSHRR DFVCSLGSS FGNTKTTIL SNSTQRTPL 
      LLRRSATLR QNGHGGTTL KDKGKNPSS PCLLQRYNC 
      NNAATTTKS IRSHHSRNS SVISRNSSR HGRIIRLEQ 
      KATKVLGVV FFTFVILWA PFFILNLLP AFCSECDKN 
      LDNWVVQLV TWLGYASSM VNPIFYTIF NKVFRQAFK 
      KVLTCKYRK TAWRPHSEA LYYAADGPG IDNENGDIR 
      RFNGPIYLH LTPGNGDRF QPPK
    CDS sequence
    • >TC013982 RA
      atggaaaac gtgttttac aaaacgacg ataatactc 
      tacaacacg acttcgaaa aacacaact gaaagctcc 
      gattccgac accgtcaac aactggtgg gctttatcg 
      gccctcatc ctcgccgtc ggcaccgct gccggcaac 
      atcctcgtc tgtcttgcg atatgttgg gaacgtcgg 
      cttcaaaac gtcaccaac tacttcctc atgtctcta 
      gccatcacc gatctgatg gtcgccatc ctcgtcatg 
      cccttagga atcctgacc ctgatcagg ggctatttt 
      cccttgcct tcgatatac tgcctggca tggatctgc 
      ctggacgta cttttttgc acagcctcg attatgcat 
      ttgtgcacc ataagcgtc gatcgatac ctctcgctc 
      agctatccg atgaaattc ggaaggaac aaatccagg 
      aggagagtc atactcaag atagttttc gtctggctg 
      ttgtcgata gccatgagc ttacccttg agtctcatg 
      tattccaaa gaccagggc actttaata aaagacggg 
      acgtgtcaa attcccgac ccgttgtat aaacttatc 
      ggttccatc atctgtttc tacatccct ttattggtc 
      atgttggtc acttatgcc ctaactgtg cgtctcctt 
      gcacaccag aggcagaat ctggggact ccggattgg 
      tccagcaac tggatggga ggaccctcg gctctagag 
      cggcgctgt acttggcga aaactccta acaaaagcc 
      aaacaaaca actcctcaa cacggccat cattcggcc 
      gcttcgaca gacaccgaa ctaacaaca atcgataac 
      cacgaatta tggatacaa gagtcggaa ccaacaccc 
      tgtaccatg tccgctttg ggtcaattc ggcgccgaa 
      atgctcaaa ctttcccgg ggactggaa gccgcctcc 
      catctgggg acccctccg ccaagacct tccccggga 
      aaaactatc ctcaaaata agctccggt gacagtctg 
      gccgactcg cattcctcg ttactgaca ccggagacg 
      tccccgtgg acctccaag agacgtcgc gcttcgacc 
      ttccacgaa ggcgacacc cgggacgaa agtccttcg 
      ccgagatgg cggaaaaga ggctcgagc tttcacgaa 
      ccgaggtca tcagacgtc tcatcggag ctttcctcc 
      gaagcgacc gaaagctcc acttttccg ctacctcca 
      ccttgcaca tgcccctat ttcggggaa aaacaagcc 
      gacaaatcg ccgccaccc aaaccggca gaagtcaaa 
      attatacca gcgacaaag ctggcacta gaagtcgaa 
      aaatcgccg ctgaaaaaa tcgctccgg aaccgcagc 
      agcgactcg tcgccgaac aacatcgtc gtcacctgg 
      gaggcttcc aggagaagc catcgacga gattttgtt 
      tgttcacta ggttcaagt ttcggcaat acgaagact 
      acaatcctg agcaattcc actcagcgc actcctctc 
      ctgctgcgc cgttcggcg acgcttcgc cagaacggc 
      cacggggga acaaccctc aaagataag ggcaaaaac 
      ccatcatcc ccttgtctt ctgcagagg tacaactgc 
      aataacgcc gccacgacg acgaaaagc atccgttcg 
      caccactca agaaactct tccgtcatt tcgaggaat 
      tcttcgcgc cacggaaga atcatccgt ttggaacag 
      aaagcgacc aaagtgtta ggtgtagtg ttcttcact 
      ttcgtgatt ctgtgggca cctttcttc attttaaac 
      ttacttccg gcgttctgt tccgagtgc gataagaat 
      ctcgataat tgggtggtg cagttggtg acgtggctg 
      ggttatgcg agttcgatg gtgaatccg attttttac 
      acaattttc aataaagtc ttcagacaa gcgtttaaa 
      aaggtgtta acgtgcaaa tatcgcaag acggcttgg 
      agaccgcac agcgaggcc ctatattac gccgccgac 
      ggtcccggc atcgacaat gagaacggc gatattaga 
      cgctttaac ggtcccatc tatctgcat ttaacgccc 
      ggaaacggc gatagattc cagccacca aaataa
    mRNA sequence
    • >TC013982 RA
      ccgaatttc agaaaatgg aaaacgtgt tttacaaaa 
      cgacgataa tactctaca acacgactt cgaaaaaca 
      caactgaaa gctccgatt ccgacaccg tcaacaact 
      ggtgggctt tatcggccc tcatcctcg ccgtcggca 
      ccgctgccg gcaacatcc tcgtctgtc ttgcgatat 
      gttgggaac gtcggcttc aaaacgtca ccaactact 
      tcctcatgt ctctagcca tcaccgatc tgatggtcg 
      ccatcctcg tcatgccct taggaatcc tgaccctga 
      tcaggggct attttccct tgccttcga tatactgcc 
      tggcatgga tctgcctgg acgtacttt tttgcacag 
      cctcgatta tgcatttgt gcaccataa gcgtcgatc 
      gatacctct cgctcagct atccgatga aattcggaa 
      ggaacaaat ccaggagga gagtcatac tcaagatag 
      ttttcgtct ggctgttgt cgatagcca tgagcttac 
      ccttgagtc tcatgtatt ccaaagacc agggcactt 
      taataaaag acgggacgt gtcaaattc ccgacccgt 
      tgtataaac ttatcggtt ccatcatct gtttctaca 
      tccctttat tggtcatgt tggtcactt atgccctaa 
      ctgtgcgtc tccttgcac accagaggc agaatctgg 
      ggactccgg attggtcca gcaactgga tgggaggac 
      cctcggctc tagagcggc gctgtactt ggcgaaaac 
      tcctaacaa aagccaaac aaacaactc ctcaacacg 
      gccatcatt cggccgctt cgacagaca ccgaactaa 
      caacaatcg ataaccacg aattatgga tacaagagt 
      cggaaccaa caccctgta ccatgtccg ctttgggtc 
      aattcggcg ccgaaatgc tcaaacttt cccggggac 
      tggaagccg cctcccatc tggggaccc ctccgccaa 
      gaccttccc cgggaaaaa ctatcctca aaataagct 
      ccggtgaca gtctggccg actcgcatt cctcgttac 
      tgacaccgg agacgtccc cgtggacct ccaagagac 
      gtcgcgctt cgaccttcc acgaaggcg acacccggg 
      acgaaagtc cttcgccga gatggcgga aaagaggct 
      cgagctttc acgaaccga ggtcatcag acgtctcat 
      cggagcttt cctccgaag cgaccgaaa gctccactt 
      ttccgctac ctccacctt gcacatgcc cctatttcg 
      gggaaaaac aagccgaca aatcgccgc cacccaaac 
      cggcagaag tcaaaatta taccagcga caaagctgg 
      cactagaag tcgaaaaat cgccgctga aaaaatcgc 
      tccggaacc gcagcagcg actcgtcgc cgaacaaca 
      tcgtcgtca cctgggagg cttccagga gaagccatc 
      gacgagatt ttgtttgtt cactaggtt caagtttcg 
      gcaatacga agactacaa tcctgagca attccactc 
      agcgcactc ctctcctgc tgcgccgtt cggcgacgc 
      ttcgccaga acggccacg ggggaacaa ccctcaaag 
      ataagggca aaaacccat catcccctt gtcttctgc 
      agaggtaca actgcaata acgccgcca cgacgacga 
      aaagcatcc gttcgcacc actcaagaa actcttccg 
      tcatttcga ggaattctt cgcgccacg gaagaatca 
      tccgtttgg aacagaaag cgaccaaag tgttaggtg 
      tagtgttct tcactttcg tgattctgt gggcacctt 
      tcttcattt taaacttac ttccggcgt tctgttccg 
      agtgcgata agaatctcg ataattggg tggtgcagt 
      tggtgacgt ggctgggtt atgcgagtt cgatggtga 
      atccgattt tttacacaa ttttcaata aagtcttca 
      gacaagcgt ttaaaaagg tgttaacgt gcaaatatc 
      gcaagacgg cttggagac cgcacagcg aggccctat 
      attacgccg ccgacggtc ccggcatcg acaatgaga 
      acggcgata ttagacgct ttaacggtc ccatctatc 
      tgcatttaa cgcccggaa acggcgata gattccagc 
      caccaaaat aacaaatca ctaatttga caattaaac 
      gaaggtgat tgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY