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Gene information
General information
TC013974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3329436..3333913
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013974
    iBeetle screen iB_05534

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013974 RA
      MVISLVKHI LRESCINNV KLNARRQNR PSIDNGTYT 
      KRPRDKFQR GSLENVSSD PWGDPQQSL RRCRSLITR 
      EDIFTNLDI QCDKPRRSQ LIPRAKLIE KSKEPRYAV 
      VRGQSLDNL SISPPDYSP ERPNVPEAV YTSISNLAS 
      PKFLIGKEQ DKRTPFEDT PGLSLPNYY PDSRYDTDN 
      TDAISLADD YSSYGDFSK KYSSCDNIS AGKWTINEP 
      ESIPYDDNI VLSANRPLY DSIRENHYA KPKTIRGPT 
      PYNSDTQNY YRRSYSSDS SEDKQKEIE ENDQISLEY 
      FETDKNLDE INENISKIL EVEEPKYSE VVEPEPIKV 
      EVEAPENEE YKTIVTVNP DETEEEKNK HSHNYLREF 
      LETQKGSKK PLQNFLSKK LSNLSRKST NLIDNRFYS 
      LPDLAVTKS LRKCEKIDR KLRKLEKNN PKKEGENRF 
      IVNIGKHFD ITANTSVPV DFELKIAKV PRKSKNKKV 
      KNRSEEQFL EAVKSLKNT LSVKLDENH NFKPEIVAF 
      QTKMDECTR EYQEKLGTM RTYWDKMVN GDKNEEAKC 
      EIVPAKIED MRKKFEPEE KEPEKPGIV QITKQLFEK 
      KTAEKGEKI SPLIKESCN YFENQFESL NPNIVEIVS 
      KDEKKHKPV TKSMSITYP EFDHVRYRV VKSDLFQKK 
      IFANCEKES QFDGLMQYL QDYSFQELL RDNNIVIIE 
      PIRTKIQHE STNKSPKTA KNVTPLLHK HKNEENPNL 
      KRHFFYHPI RVNKEVNDD ELPNPDTVK QVRLFFEGG 
      LKRSQSSDF ESNRNEEKC EKNADPDKD HCSATDSNT 
      SNLSDFGSQ ENLYDSLTE HCCERQYVS EDILEKIRE 
      CGTTVTYYG GRVVKKQSG QPVLTKAIM EEIRSNEMK 
      NSECGCRKD GYQGVKFKL LKSNSCNSR LELVGTNDL 
      KESKEKYLK NRQVNLDKN TINETIKKN MDFQPRIIG 
      EERKMTQWG DLKEKTYNG ISFNNRKKV TYDYHNYDS 
      IKQKTKKID DMEFEPYEV A
    CDS sequence
    • >TC013974 RA
      atggttatc tccttggtt aaacacatt ttacgagag 
      tcgtgcata aacaacgtg aaactcaac gctcgacgt 
      cagaaccga ccctcgatc gacaacggc acctacacc 
      aagcgcccc cgtgataaa ttccagagg ggtagtcta 
      gagaacgtc tccagcgac ccctggggg gacccacaa 
      cagtccttg aggaggtgt cgaagtcta atcacacga 
      gaggatatt ttcaccaat ttggacatc cagtgtgat 
      aaaccgaga cgatcgcag ctgattcct cgagcgaag 
      ttgatcgaa aaatcaaag gaaccaagg tacgctgtt 
      gtccgggga caaagtttg gacaatctt agtataagt 
      cccccggat tattccccg gaaaggccg aacgttccg 
      gaagccgtc tacacgtca ataagtaac ttggcgagt 
      cccaaattc cttattggc aaagagcaa gacaagagg 
      actccgttt gaggatact ccaggctta agtcttcct 
      aactattat cctgattcg agatacgac actgataac 
      accgatgcg atctcttta gctgatgac tactcatcg 
      tatggcgat ttcagtaaa aagtactcc tcgtgtgat 
      aatatttca gcagggaag tggacgata aacgaacct 
      gagagtatc ccttacgac gataatata gtactaagt 
      gcaaatagg cctttatac gattccatc cgggagaat 
      cactacgct aaaccaaag acaataaga ggcccaact 
      ccctacaat agtgacaca cagaactac tatagaaga 
      agctattct tctgattct tccgaagat aagcagaaa 
      gaaatcgaa gagaacgac caaatcagt ttggagtac 
      ttcgaaacc gataaaaac cttgacgaa atcaacgaa 
      aacatcagt aaaatccta gaggttgag gaaccaaag 
      tacagtgaa gtggtcgaa ccagaaccc atcaaagtc 
      gaagtcgaa gcaccagag aacgaagaa tacaagacc 
      atagtgacc gtcaatcct gacgaaacc gaagaagag 
      aaaaacaag cacagtcat aactacttg cgcgagttt 
      ctcgaaacg caaaaaggg tccaaaaag cccttgcag 
      aatttcctc agcaagaaa ttatcaaac ttgtcccgg 
      aaaagtacg aatctgatc gataatcgt ttctactcc 
      ctcccagac ctcgccgtg acgaagagt ttgcgaaaa 
      tgcgaaaaa atcgatcgt aagttgcgc aaattagag 
      aaaaataac cccaagaaa gagggcgaa aatcgcttc 
      attgtcaac attggcaaa catttcgac atcacggcc 
      aacactagc gtccccgtc gacttcgaa ctaaaaatc 
      gcgaaagta ccgaggaaa agcaagaac aagaaagtg 
      aagaaccgg tccgaggag caatttctc gaagccgtg 
      aagagcttg aagaacacc ttaagtgtg aaactcgac 
      gagaatcat aattttaag cctgaaatt gtggcgttt 
      cagactaag atggacgag tgcacgagg gagtaccag 
      gagaagttg ggcaccatg cggacgtac tgggacaaa 
      atggtcaat ggggacaaa aacgaagaa gcgaagtgt 
      gagattgtt ccggcgaaa atcgaagac atgaggaag 
      aaatttgag ccggaggaa aaagagccg gaaaagccc 
      ggaatcgtg caaatcacc aagcaactc ttcgagaag 
      aaaaccgcc gaaaagggc gaaaaaatc tcacccttg 
      atcaaggaa tcgtgcaat tatttcgaa aatcaattc 
      gagagcctc aaccccaac atcgtcgaa atcgtttcg 
      aaggacgaa aagaagcac aaacccgtg accaaatca 
      atgagcatc acttatcca gagttcgac catgtgcgc 
      taccgggtg gtcaaatcc gacctcttc cagaagaaa 
      atcttcgcc aactgcgaa aaggagagc cagttcgat 
      ggtttgatg caatatttg caagactac agtttccag 
      gagttgctt cgtgacaac aacatcgtg ataatcgag 
      ccaatccgg acaaaaatc cagcacgaa tccactaat 
      aagagccca aagaccgca aaaaacgtc acacccttg 
      ttgcacaag cacaaaaat gaggaaaac cccaacttg 
      aaacgccac ttcttctac cacccgatc cgggtgaac 
      aaggaagtg aacgatgac gaattacca aaccctgat 
      accgtcaaa caagtccgg ctgtttttc gaagggggg 
      ttgaaaaga tcgcagagt tcggatttt gagagcaac 
      aggaacgag gaaaagtgc gaaaaaaac gccgatcct 
      gataaagat cattgttcg gctaccgat tcaaacacc 
      tcgaacttg tccgatttt ggaagccag gaaaacctt 
      tacgattct ttgaccgag cattgttgc gaacgccaa 
      tacgtcagt gaggacatc ctggagaaa atccgggaa 
      tgcgggacc acagtgacg tattacggg ggccgtgtg 
      gtgaagaaa cagtcggga caacccgta ctcaccaag 
      gcaataatg gaggaaatc cgaagtaac gaaatgaag 
      aacagtgag tgtgggtgc aggaaggat ggttatcag 
      ggggttaag ttcaagttg ctcaagtcg aacagttgc 
      aatagtcgc ttggagttg gttgggacc aatgatttg 
      aaagagtcc aaggagaag tatttgaag aaccgccag 
      gtcaatctt gacaagaac acaatcaac gaaacgatc 
      aagaagaac atggatttc caaccgagg ataatcgga 
      gaggagcgc aaaatgacg caatggggc gacctcaag 
      gaaaaaacc tacaacggg atcagtttc aacaacagg 
      aagaaagtg acgtacgat taccacaat tacgactcg 
      atcaagcaa aagacgaaa aaaatcgac gacatggaa 
      ttcgagccg tatgaggtg gcttga
    mRNA sequence
    • >TC013974 RA
      tgtgtgtga tttggtgcc gctccgttt ccactaatc 
      cgaaaaatt ttaatgaca atcgaccgg atttccttc 
      cgggatcac gcgtaccgt aaatccgcg tctggagtg 
      gactggaat tttcactct tcgggtaat tgcgttgat 
      tttcttgat gatgatgat gacgataat cgcccgatt 
      agacgtaat tggcccaga tttttacaa ttatggata 
      attaccggc gacttttcc gaaaaaatt ttcgcgcaa 
      acatggtta tctccttgg ttaaacaca ttttacgag 
      agtcgtgca taaacaacg tgaaactca acgctcgac 
      gtcagaacc gaccctcga tcgacaacg gcacctaca 
      ccaagcgcc cccgtgata aattccaga ggggtagtc 
      tagagaacg tctccagcg acccctggg gggacccac 
      aacagtcct tgaggaggt gtcgaagtc taatcacac 
      gagaggata ttttcacca atttggaca tccagtgtg 
      ataaaccga gacgatcgc agctgattc ctcgagcga 
      agttgatcg aaaaatcaa aggaaccaa ggtacgctg 
      ttgtccggg gacaaagtt tggacaatc ttagtataa 
      gtcccccgg attattccc cggaaaggc cgaacgttc 
      cggaagccg tctacacgt caataagta acttggcga 
      gtcccaaat tccttattg gcaaagagc aagacaaga 
      ggactccgt ttgaggata ctccaggct taagtcttc 
      ctaactatt atcctgatt cgagatacg acactgata 
      acaccgatg cgatctctt tagctgatg actactcat 
      cgtatggcg atttcagta aaaagtact cctcgtgtg 
      ataatattt cagcaggga agtggacga taaacgaac 
      ctgagagta tcccttacg acgataata tagtactaa 
      gtgcaaata ggcctttat acgattcca tccgggaga 
      atcactacg ctaaaccaa agacaataa gaggcccaa 
      ctccctaca atagtgaca cacagaact actatagaa 
      gaagctatt cttctgatt cttccgaag ataagcaga 
      aagaaatcg aagagaacg accaaatca gtttggagt 
      acttcgaaa ccgataaaa accttgacg aaatcaacg 
      aaaacatca gtaaaatcc tagaggttg aggaaccaa 
      agtacagtg aagtggtcg aaccagaac ccatcaaag 
      tcgaagtcg aagcaccag agaacgaag aatacaaga 
      ccatagtga ccgtcaatc ctgacgaaa ccgaagaag 
      agaaaaaca agcacagtc ataactact tgcgcgagt 
      ttctcgaaa cgcaaaaag ggtccaaaa agcccttgc 
      agaatttcc tcagcaaga aattatcaa acttgtccc 
      ggaaaagta cgaatctga tcgataatc gtttctact 
      ccctcccag acctcgccg tgacgaaga gtttgcgaa 
      aatgcgaaa aaatcgatc gtaagttgc gcaaattag 
      agaaaaata accccaaga aagagggcg aaaatcgct 
      tcattgtca acattggca aacatttcg acatcacgg 
      ccaacacta gcgtccccg tcgacttcg aactaaaaa 
      tcgcgaaag taccgagga aaagcaaga acaagaaag 
      tgaagaacc ggtccgagg agcaatttc tcgaagccg 
      tgaagagct tgaagaaca ccttaagtg tgaaactcg 
      acgagaatc ataatttta agcctgaaa ttgtggcgt 
      ttcagacta agatggacg agtgcacga gggagtacc 
      aggagaagt tgggcacca tgcggacgt actgggaca 
      aaatggtca atggggaca aaaacgaag aagcgaagt 
      gtgagattg ttccggcga aaatcgaag acatgagga 
      agaaatttg agccggagg aaaaagagc cggaaaagc 
      ccggaatcg tgcaaatca ccaagcaac tcttcgaga 
      agaaaaccg ccgaaaagg gcgaaaaaa tctcaccct 
      tgatcaagg aatcgtgca attatttcg aaaatcaat 
      tcgagagcc tcaacccca acatcgtcg aaatcgttt 
      cgaaggacg aaaagaagc acaaacccg tgaccaaat 
      caatgagca tcacttatc cagagttcg accatgtgc 
      gctaccggg tggtcaaat ccgacctct tccagaaga 
      aaatcttcg ccaactgcg aaaaggaga gccagttcg 
      atggtttga tgcaatatt tgcaagact acagtttcc 
      aggagttgc ttcgtgaca acaacatcg tgataatcg 
      agccaatcc ggacaaaaa tccagcacg aatccacta 
      ataagagcc caaagaccg caaaaaacg tcacaccct 
      tgttgcaca agcacaaaa atgaggaaa accccaact 
      tgaaacgcc acttcttct accacccga tccgggtga 
      acaaggaag tgaacgatg acgaattac caaaccctg 
      ataccgtca aacaagtcc ggctgtttt tcgaagggg 
      ggttgaaaa gatcgcaga gttcggatt ttgagagca 
      acaggaacg aggaaaagt gcgaaaaaa acgccgatc 
      ctgataaag atcattgtt cggctaccg attcaaaca 
      cctcgaact tgtccgatt ttggaagcc aggaaaacc 
      tttacgatt ctttgaccg agcattgtt gcgaacgcc 
      aatacgtca gtgaggaca tcctggaga aaatccggg 
      aatgcggga ccacagtga cgtattacg ggggccgtg 
      tggtgaaga aacagtcgg gacaacccg tactcacca 
      aggcaataa tggaggaaa tccgaagta acgaaatga 
      agaacagtg agtgtgggt gcaggaagg atggttatc 
      agggggtta agttcaagt tgctcaagt cgaacagtt 
      gcaatagtc gcttggagt tggttggga ccaatgatt 
      tgaaagagt ccaaggaga agtatttga agaaccgcc 
      aggtcaatc ttgacaaga acacaatca acgaaacga 
      tcaagaaga acatggatt tccaaccga ggataatcg 
      gagaggagc gcaaaatga cgcaatggg gcgacctca 
      aggaaaaaa cctacaacg ggatcagtt tcaacaaca 
      ggaagaaag tgacgtacg attaccaca attacgact 
      cgatcaagc aaaagacga aaaaaatcg acgacatgg 
      aattcgagc cgtatgagg tggcttgag gtgcaacga 
      atttcgacg atttaggtg tatcaggtt tagggtgta 
      tgacttgta catgaataa atatttgta gcaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY