Status
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Gene information
General information
TC033031
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013969,TC013968
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3284885..3291371
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033031
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05532

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033031 RA
      MRKLIVATC LITLVWGYH VKTQDFDVA IESIEFTKI 
      PAKYNLNFA NEEVLKEII GGIKRPKSG TEAILNEYA 
      DKIFQNIQN LMIHGGFDP IEMPDMHTG FNYTLIITY 
      HGELDLTHG WLSDLSTIH RSGDVVVSY SSNTKYLEI 
      TVPIAFDDL QFTYDYSAK IMGLGPTGG IEGKANSVG 
      VRVKIGFNV ETLEASLDE FSITNSGRI TFHFNGNAL 
      IDWLLNLLT DVTTILLKR VILLVVENV IKGGLSAAI 
      DAINEVING TEIVMCEMK LIVVITCLI SEILSHHIN 
      FEETECPAR YKLDETVFL NQKFFQKLV EDIKFNNNR 
      TVTNDYVDE IFFNIRNLM IHEDFDPIQ LPGVTTNLS 
      NTFIVTYQG ILNLSQGWL SDLSTIHRK GEIISYNYH 
      NKYFMTIPV AFKEMHFTY DYDTTIMGL ESQGGIEGK 
      ISYLSAKVK IGFDSQKLV TFLEDFHID NSGIVSFHF 
      TGNVLTDWL LNLMKHTTS ELLQKVTFF ILENVLCGG 
      LESAFRTMN KMLNPTGPS MQLL
    CDS sequence
    • >TC033031 RA
      atgcgaaaa ttaattgta gctacatgt ttgataacc 
      cttgtgtgg ggttaccac gtcaaaacc caagatttt 
      gatgtggcc attgaaagt attgaattt acgaaaatt 
      ccggcaaag tataactta aattttgca aatgaagaa 
      gttctaaag gaaattatt ggaggaata aagcgaccg 
      aagagtgga actgaagca atcttgaat gaatacgcg 
      gataaaatt ttccaaaat atccaaaat ctgatgatt 
      catggcggt tttgaccca attgaaatg cccgacatg 
      cacacgggt ttcaactat actctgatc atcacgtac 
      catggcgag ctggacttg acccatggc tggttgagc 
      gacttgtcg accatccat cgaagtggc gatgttgtc 
      gtttcctac agttccaat accaagtat ttggaaatc 
      acagttcca atagccttc gatgactta caattcact 
      tatgactac agtgccaaa atcatgggc ttgggaccc 
      acgggtggt atcgaaggt aaagccaat tcggtgggt 
      gtgcgagtg aaaattggg ttcaatgtt gagactttg 
      gaggcgtct ttagatgag tttagtatc acgaattcg 
      ggacgtatc acttttcat tttaatgga aacgcactc 
      attgactgg cttctcaac ttgttgact gacgtgaca 
      acaattctt ttaaaacga gttattttg ttagtggtc 
      gaaaatgtt attaaagga ggtttgtca gcggctatt 
      gatgcgata aatgaggtg attaatgga actgagata 
      gtgatgtgc gaaatgaag ctaattgtg gtgatcact 
      tgtttaatt tcggaaata ttaagccat cacatcaat 
      tttgaagaa accgaatgt ccggcaaga tacaaatta 
      gacgaaacg gttttctta aaccaaaaa ttttttcaa 
      aaacttgtc gaagacatt aaattcaat aacaatcgc 
      acagtaacg aatgactat gtcgacgaa attttcttc 
      aatattcgc aatttgatg atacatgaa gactttgat 
      cccatacaa ttgcctggt gttacgaca aacctgtct 
      aatacgttc attgtgacg tatcaggga atcttgaac 
      ctgtcccaa gggtggttg agtgactta tcgaccatc 
      catcgtaaa ggcgaaatt atatcgtac aattatcac 
      aataaatat ttcatgaca ataccagtc gctttcaaa 
      gaaatgcac ttcacttat gactacgat accacaatt 
      atgggcctg gaatctcaa gggggaatt gaaggaaaa 
      atcagttac ttatcggca aaagtaaaa atcgggttc 
      gattctcaa aaattagtg acgttttta gaagacttc 
      cacatagat aattcaggg attgtttct ttccatttc 
      accggaaat gtcttaact gactggctc ctcaattta 
      atgaaacac acaacgagc gagcttttg caaaaagtc 
      actttcttt atccttgaa aatgttctt tgtggagga 
      ctggagtcg gccttcaga accatgaat aaaatgttg 
      aaccccact gggccttct atgcaactc ctgtag
    mRNA sequence
    • >TC033031 RA
      ccaaaagac gcaaatttt tgaaaatat cttatcgtt 
      ttatcgatt ctcatgtat aaagtcgcc aacaagtaa 
      acaatcaat aacaaaatg cgaaaatta attgtagct 
      acatgtttg ataaccctt gtgtggggt taccacgtc 
      aaaacccaa gattttgat gtggccatt gaaagtatt 
      gaatttacg aaaattccg gcaaagtat aacttaaat 
      tttgcaaat gaagaagtt ctaaaggaa attattgga 
      ggaataaag cgaccgaag agtggaact gaagcaatc 
      ttgaatgaa tacgcggat aaaattttc caaaatatc 
      caaaatctg atgattcat ggcggtttt gacccaatt 
      gaaatgccc gacatgcac acgggtttc aactatact 
      ctgatcatc acgtaccat ggcgagctg gacttgacc 
      catggctgg ttgagcgac ttgtcgacc atccatcga 
      agtggcgat gttgtcgtt tcctacagt tccaatacc 
      aagtatttg gaaatcaca gttccaata gccttcgat 
      gacttacaa ttcacttat gactacagt gccaaaatc 
      atgggcttg ggacccacg ggtggtatc gaaggtaaa 
      gccaattcg gtgggtgtg cgagtgaaa attgggttc 
      aatgttgag actttggag gcgtcttta gatgagttt 
      agtatcacg aattcggga cgtatcact tttcatttt 
      aatggaaac gcactcatt gactggctt ctcaacttg 
      ttgactgac gtgacaaca attctttta aaacgagtt 
      attttgtta gtggtcgaa aatgttatt aaaggaggt 
      ttgtcagcg gctattgat gcgataaat gaggtgatt 
      aatggaact gagatagtg atgtgcgaa atgaagcta 
      attgtggtg atcacttgt ttaatttcg gaaatatta 
      agccatcac atcaatttt gaagaaacc gaatgtccg 
      gcaagatac aaattagac gaaacggtt ttcttaaac 
      caaaaattt tttcaaaaa cttgtcgaa gacattaaa 
      ttcaataac aatcgcaca gtaacgaat gactatgtc 
      gacgaaatt ttcttcaat attcgcaat ttgatgata 
      catgaagac tttgatccc atacaattg cctggtgtt 
      acgacaaac ctgtctaat acgttcatt gtgacgtat 
      cagggaatc ttgaacctg tcccaaggg tggttgagt 
      gacttatcg accatccat cgtaaaggc gaaattata 
      tcgtacaat tatcacaat aaatatttc atgacaata 
      ccagtcgct ttcaaagaa atgcacttc acttatgac 
      tacgatacc acaattatg ggcctggaa tctcaaggg 
      ggaattgaa ggaaaaatc agttactta tcggcaaaa 
      gtaaaaatc gggttcgat tctcaaaaa ttagtgacg 
      tttttagaa gacttccac atagataat tcagggatt 
      gtttctttc catttcacc ggaaatgtc ttaactgac 
      tggctcctc aatttaatg aaacacaca acgagcgag 
      cttttgcaa aaagtcact ttctttatc cttgaaaat 
      gttctttgt ggaggactg gagtcggcc ttcagaacc 
      atgaataaa atgttgaac cccactggg ccttctatg 
      caactcctg tagttttgc ttaattatt aaaaaaaat 
      tgtgactcc acattttgc aaagaatca tgttttgta 
      aaaataaaa ttttggaaa aaaaggata tttaaaaca 
      acataccta tacacacac tgataagag ataacagtt 
      tttaattca ttagtcatc attttgttg taattcgtg 
      aaatgagag aaatgtaat gtcagtaag gacgatcag 
      tgaaaggtg tgtccaaaa atggtctcg tctagaaag 
      ttggtagag actgtgtgt cttttgttg taagttgga 
      tctttgggc ttatttata aacaaaaaa gaattaatt 
      ttagaactt gcggtaaat tatctatta taataagca 
      gtgaaatgt atctaacct tacattata aaattttat 
      ttttggagc ccacttatc gaactagca gagataaaa 
      aatattctt tgggaccat gccttgaag tttttcttt 
      atatttgtt atcctagta actgtagta atttgtatt 
      tgttgtcaa ttcaaatcg attgatggc gaaaagaaa 
      aagaatgaa agtaacaac atgagcagt acttacaac 
      ttgatttaa cgacccgac aagaagtat cgaaaaata 
      actgcaaaa aaggacatg atgaaaatt gaatggtac 
      cttatactg tgacgttgc attagatct tagataatg 
      atatttaac gaagtagat aattgttta aaatatata 
      gattactat taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY