Status
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Gene information
General information
TC033026
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013956,TC013954
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2916742..3056347
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033026
    iBeetle screen iB_05531

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033026 RA
      GQGPRCHHE TLRWRASPD FQETINPAR LVFFRSRGI 
      VWILIFSTL ILSSQCNSF MKNAKISPR IVQTRYGRL 
      QGLVVPMHR YLKPIEVFL GVPYATPPV QSNRFSPTR 
      TPSPWDGVR ISDKMGPVC PQKLPDISN ETAALEKMP 
      KGRLEYLKR LLPYLKNQS EDCLYLNIY APAQVSNEA 
      RHPVIVYIH GESFEWNSG NPYDGSVLA AYAELVVVT 
      LNYRLGILG FLNANPAPH LKARVANYG LMDQIAALH 
      WIQQNIALF GGDPDNVTL AGHGSGAAC INFLMISPT 
      VMPGLFHRA ILLSGSALS SWALVEDPV NYAVKLARE 
      VNCTIPEDV GKDHEAIVD CLRDTPLHD LLQADVTPP 
      AYLSAFGPS VDGVVIKAD FAKELVTYF KPSDLQSFV 
      GPVNANMKR SEATLTPRG SNPYDLLFG VVTSEALWR 
      FSATDIQAG FEGERRDKI IRTYVRNAY TYHLSEIFF 
      TVVNEYTDW ERTVQHPIN TRDAAVAAL SDAQFVAPL 
      VQTGDLLSA KPPQPGQEP SGSKTFFYV FDYQTKDGD 
      YPQRMGTVH GEELPYFFG APLVDGFNH FPKNYTRSE 
      LALAEAVII YIANFARTG NPNEQHKQE PILAASKER 
      NRFRSIVWD EYDSVHQKY LEIGMKPRM KNHFRAHQL 
      SVWLRLIPE LHRAGMEDV VARHNLFRN HNNADLYDG 
      AVRPDPLSR VSYYDPTME LFRRRPNSS LSGLEAPTT 
      MDTIVTTCV NVVSSGNFN HQTYQNQNT STDTLASLE 
      AAGYAAYST ALSVTIAIG CSLLILNVL IFAGVYYQR 
      DKTRMEVKS LQQQQSRNQ ATFETISKH GHYHMGHSQ 
      SANVIVDVE QDTSAMIMA ANAQHDKQH LCPSNIQMS 
      NTLKAPPPS PSVMMQNCM TLPKNASSY GQNYNPGSM 
      TLPKNATLL NNTACVIAE MQQQGLVQP PNGNATMPM 
      SVPRPPPPP RTKSPPESQ PLLANNKGN MRVPQAAMS 
      EMRV
    CDS sequence
    • >TC033026 RA
      gggcaaggt ccccggtgc caccatgag actttgcga 
      tggcgagcc agtccggat tttcaagaa acaattaat 
      ccggcccgc ttagtgttt tttcgtagc aggggaatc 
      gtctggatt ttaattttc tccactttg atactttcg 
      agccaatgc aactcgttt atgaagaac gccaagata 
      agcccgagg atcgtgcag acgcggtat gggcggctg 
      cagggcctg gtggtgccc atgcaccgc tacctcaaa 
      cccatcgag gtcttcctg ggcgtccca tatgccacg 
      ccgccagtc cagtccaac agatttagc cccacacgg 
      acgcccagc ccctgggac ggagtccgc atttcggac 
      aagatgggt ccagtgtgc ccacagaag ctcccggat 
      atcagcaac gaaaccgca gctttggag aagatgccc 
      aaggggcgg ctggagtat ttgaaacgg cttctccct 
      tacttgaaa aaccagagc gaagactgc ctttatttg 
      aacatttat gcccctgca caagtttcc aacgaagct 
      cgccatcct gtgatagtc tacatacat ggcgagtcc 
      ttcgagtgg aactcgggc aatccctac gacgggagc 
      gtcctagcg gcgtatgcc gaactcgtc gtagtcaca 
      ctaaactat aggctagga atcttaggt tttttaaac 
      gcgaacccc gccccccac ctcaaagcc cgcgtggcc 
      aactacggc ctcatggac cagatcgcc gctctgcac 
      tggatccag cagaacatc gccctcttc ggcggagac 
      ccagacaac gtgactctg gccggccac ggctccgga 
      gctgcttgc atcaacttc ctcatgatt tcacccaca 
      gtcatgcct ggccttttc caccgcgca atcctcctc 
      tccggctcc gccctgtct tcgtgggcc ctcgtcgaa 
      gacccagtt aactacgcc gtcaagctg gcccgcgaa 
      gtcaactgc accatcccc gaggacgtc ggcaaggac 
      cacgaagct atcgtcgac tgcctccgg gacactccg 
      ctccacgac ctcctccaa gccgacgtc actccaccg 
      gcgtacctg agtgcgttt gggccctcc gtcgacggt 
      gtcgtgatc aaagcggac ttcgccaag gagctggtg 
      acgtatttc aaaccctcg gacttgcag agcttcgta 
      gggccagtg aacgccaac atgaagagg agcgaagcg 
      acgctgact ccgagaggg agtaacccc tacgacctt 
      ttgttcggg gtggtgacc agcgaggcg ctgtggagg 
      ttctcagcg acggatatc caggcgggg tttgagggc 
      gaaaggcgg gacaaaatc ataaggact tatgtgaga 
      aacgcgtat acgtatcat ttaagtgag attttcttt 
      acggtagtg aatgagtac acggattgg gagaggacg 
      gtccaacat ccgattaac acaagagac gcggcagtg 
      gcggcgctg agtgatgca cagtttgtg gcgccactg 
      gtgcaaacc ggggatttg ttaagtgcc aaaccgccg 
      caacccgga caagaaccc tcagggtcc aagactttc 
      ttctacgtg tttgactac caaaccaaa gatggggac 
      tatccccag cgcatgggg acagtccac ggcgaggag 
      ctcccctac ttctttggg gcgcctttg gtggacggc 
      ttcaaccac ttcccgaag aactacacc cggtccgaa 
      ctggctctg gccgaggcc gtcatcatc tacatcgcc 
      aatttcgca cggacgggt aaccccaac gagcagcac 
      aagcaggaa cccatcctg gccgcctcc aaggaacgc 
      aaccgcttc aggtccatc gtctgggac gaatacgac 
      tcagtccat caaaagtac ctcgagata ggcatgaag 
      ccccggatg aagaaccat ttccgggcc catcaatta 
      agtgtgtgg ctgcgtctg atcccggag ctgcaccgc 
      gccggaatg gaggacgtc gtggccaga cacaatctc 
      ttcaggaat cacaataac gcagacttg tacgacggg 
      gccgtccgc cctgacccc ttatctcga gtcagttat 
      tacgacccc acgatggag ctattccgc cgccgaccc 
      aactccagt ctttccggg ctagaagcc cccacaacg 
      atggacacg atcgtaacg acttgtgtc aacgtcgtt 
      tcatcggga aatttcaac caccaaacc taccagaac 
      caaaacacc tcgactgac accctggcc agtttggag 
      gcggcggga tacgccgcc tacagcacg gctttgagt 
      gtcaccatc gcgattggg tgctcgttg ctgatttta 
      aatgtactg attttcgcc ggagtgtac taccagagg 
      gacaagacg cgcatggag gtcaagtcg ctccagcag 
      caacaaagt cggaatcaa gccacgttt gagaccatc 
      tccaagcat gggcactac catatggga cattcgcag 
      agcgcgaat gtcatcgtg gacgtggaa caggacacc 
      tcggcgatg atcatggcg gcgaatgcc caacacgat 
      aaacagcat ctgtgcccc agcaacata caaatgtcc 
      aacacgttg aaggcgccg ccccccagc ccgagcgtg 
      atgatgcag aactgcatg accctgccg aagaacgcc 
      tccagctac ggccagaac tacaaccct gggagtatg 
      accctcccc aagaacgcc acattgctg aacaacacg 
      gcgtgcgtg atcgccgag atgcagcag cagggcttg 
      gtgcagccc cccaatggc aacgcgacg atgccgatg 
      agcgtgcct cggccgccg ccgccccct cggaccaaa 
      agtccgccg gaaagccag cctttgttg gccaacaac 
      aaagggaac atgcgagtt ccacaagcg gccatgagt 
      gagatgcgc gtgtga
    mRNA sequence
    • >TC033026 RA
      ggggcaagg tccccggtg ccaccatga gactttgcg 
      atggcgagc cagtccgga ttttcaaga aacaattaa 
      tccggcccg cttagtgtt ttttcgtag caggggaat 
      cgtctggat tttaatttt ctccacttt gatactttc 
      gagccaatg caactcgtt tatgaagaa cgccaagat 
      aagcccgag gatcgtgca gacgcggta tgggcggct 
      gcagggcct ggtggtgcc catgcaccg ctacctcaa 
      acccatcga ggtcttcct gggcgtccc atatgccac 
      gccgccagt ccagtccaa cagatttag ccccacacg 
      gacgcccag cccctggga cggagtccg catttcgga 
      caagatggg tccagtgtg cccacagaa gctcccgga 
      tatcagcaa cgaaaccgc agctttgga gaagatgcc 
      caaggggcg gctggagta tttgaaacg gcttctccc 
      ttacttgaa aaaccagag cgaagactg cctttattt 
      gaacattta tgcccctgc acaagtttc caacgaagc 
      tcgccatcc tgtgatagt ctacataca tggcgagtc 
      cttcgagtg gaactcggg caatcccta cgacgggag 
      cgtcctagc ggcgtatgc cgaactcgt cgtagtcac 
      actaaacta taggctagg aatcttagg ttttttaaa 
      cgcgaaccc cgcccccca cctcaaagc ccgcgtggc 
      caactacgg cctcatgga ccagatcgc cgctctgca 
      ctggatcca gcagaacat cgccctctt cggcggaga 
      cccagacaa cgtgactct ggccggcca cggctccgg 
      agctgcttg catcaactt cctcatgat ttcacccac 
      agtcatgcc tggcctttt ccaccgcgc aatcctcct 
      ctccggctc cgccctgtc ttcgtgggc cctcgtcga 
      agacccagt taactacgc cgtcaagct ggcccgcga 
      agtcaactg caccatccc cgaggacgt cggcaagga 
      ccacgaagc tatcgtcga ctgcctccg ggacactcc 
      gctccacga cctcctcca agccgacgt cactccacc 
      ggcgtacct gagtgcgtt tgggccctc cgtcgacgg 
      tgtcgtgat caaagcgga cttcgccaa ggagctggt 
      gacgtattt caaaccctc ggacttgca gagcttcgt 
      agggccagt gaacgccaa catgaagag gagcgaagc 
      gacgctgac tccgagagg gagtaaccc ctacgacct 
      tttgttcgg ggtggtgac cagcgaggc gctgtggag 
      gttctcagc gacggatat ccaggcggg gtttgaggg 
      cgaaaggcg ggacaaaat cataaggac ttatgtgag 
      aaacgcgta tacgtatca tttaagtga gattttctt 
      tacggtagt gaatgagta cacggattg ggagaggac 
      ggtccaaca tccgattaa cacaagaga cgcggcagt 
      ggcggcgct gagtgatgc acagtttgt ggcgccact 
      ggtgcaaac cggggattt gttaagtgc caaaccgcc 
      gcaacccgg acaagaacc ctcagggtc caagacttt 
      cttctacgt gtttgacta ccaaaccaa agatgggga 
      ctatcccca gcgcatggg gacagtcca cggcgagga 
      gctccccta cttctttgg ggcgccttt ggtggacgg 
      cttcaacca cttcccgaa gaactacac ccggtccga 
      actggctct ggccgaggc cgtcatcat ctacatcgc 
      caatttcgc acggacggg taaccccaa cgagcagca 
      caagcagga acccatcct ggccgcctc caaggaacg 
      caaccgctt caggtccat cgtctggga cgaatacga 
      ctcagtcca tcaaaagta cctcgagat aggcatgaa 
      gccccggat gaagaacca tttccgggc ccatcaatt 
      aagtgtgtg gctgcgtct gatcccgga gctgcaccg 
      cgccggaat ggaggacgt cgtggccag acacaatct 
      cttcaggaa tcacaataa cgcagactt gtacgacgg 
      ggccgtccg ccctgaccc cttatctcg agtcagtta 
      ttacgaccc cacgatgga gctattccg ccgccgacc 
      caactccag tctttccgg gctagaagc ccccacaac 
      gatggacac gatcgtaac gacttgtgt caacgtcgt 
      ttcatcggg aaatttcaa ccaccaaac ctaccagaa 
      ccaaaacac ctcgactga caccctggc cagtttgga 
      ggcggcggg atacgccgc ctacagcac ggctttgag 
      tgtcaccat cgcgattgg gtgctcgtt gctgatttt 
      aaatgtact gattttcgc cggagtgta ctaccagag 
      ggacaagac gcgcatgga ggtcaagtc gctccagca 
      gcaacaaag tcggaatca agccacgtt tgagaccat 
      ctccaagca tgggcacta ccatatggg acattcgca 
      gagcgcgaa tgtcatcgt ggacgtgga acaggacac 
      ctcggcgat gatcatggc ggcgaatgc ccaacacga 
      taaacagca tctgtgccc cagcaacat acaaatgtc 
      caacacgtt gaaggcgcc gccccccag cccgagcgt 
      gatgatgca gaactgcat gaccctgcc gaagaacgc 
      ctccagcta cggccagaa ctacaaccc tgggagtat 
      gaccctccc caagaacgc cacattgct gaacaacac 
      ggcgtgcgt gatcgccga gatgcagca gcagggctt 
      ggtgcagcc ccccaatgg caacgcgac gatgccgat 
      gagcgtgcc tcggccgcc gccgccccc tcggaccaa 
      aagtccgcc ggaaagcca gcctttgtt ggccaacaa 
      caaagggaa catgcgagt tccacaagc ggccatgag 
      tgagatgcg cgtgtgata atatcaagt gctctagtg 
      accattcgt ccggatttt ggcgggcct aaaacgcgg 
      cctcattcc tacgtgtac agaagattt attatggat 
      gtttctgtg tataagtgt gtatttttt caaattgtt 
      tcagagtct attgcgttg gggagagtg aataaaaag 
      ttttgagtt tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY