Status
Please wait ...
Gene information
General information
TC013945 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2700490..2750487
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013945
    iBeetle screen iB_05529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013945 RA
      MMSGAPIPD VGQSWLKSN SSLAGFEVN SSEQLTTPT 
      PTDINSFYF YKTEQFTVL WVLFTMIVL GNSAVLVAL 
      LVSKSRKSR MNFFICQLA IADLAVGLI SVSTDIAWR 
      ITVAWYAGN VLCKIIRFL QAVVTYSST YVLVALSID 
      RYDAITHPM NFSGSWKRA RVLVMLAWL LSILFSLPT 
      VFLFEEKQV QSMPQCWID LQTWQWKVY ITLVALVLF 
      VFPALIISA CYAVIVRTI WSKSKLLIP VGHIPIRQC 
      DDHRDRPPR HFHEEHDTR RASSRGIIP RAKIKTVKM 
      TLVIVFVFV LCWSPYIVF DLLQVYGHI PKTQTNIAI 
      ATLIQSLAP LNSAANPVI YCLFSTHLC RNLRKLPPF 
      TWISNCLSL CFPGVQGSG HCLGYGSGD SSGTVTTSL 
      THSTRRSGS AASLRHTIR LQPALNNTH KVAISVV
    • >TC013945 RB
      MMSGAPIPD VGQSWLKSN SSLAGFEVN SSEQLTTPT 
      PTDINSFYF YKTEQFTVL WVLFTMIVL GNSAVLVAL 
      LVSKSRKSR MNFFICQLA IADLAVGLI SVSTDIAWR 
      ITVAWYAGN VLCKIIRFL QAVVTYSST YVLVALSID 
      RYDAITHPM NFSGSWKRA RVLVMLAWL LSILFSLPT 
      VFLFEEKQV QSMPQCWID LQTWQWKVY ITLVALVLF 
      VFPALIISA CYAVIVRTI WSKSKLLIP VGHIPIRQC 
      DDHRDRPPR HFHEEHDTR RASSRGIIP RAKIKTVKM 
      TLVIVFVFV LCWSPYIVF DLLQVYGHI PKTQTNIAI 
      ATLIQSLAP LNSAANPVI YCLFSTHLC RNLRCSSLL 
      KHLNFDPCR KLPPFTWIS NCLSLCFPG VQGSGHCLG 
      YGSGDSSGT VTTSLTHST RRSGSAASL RHTIRLQPA 
      LNNTHKVAI SVV
    CDS sequence
    • >TC013945 RA
      atgatgagt ggagccccg attccggat gtcgggcaa 
      agctggctg aaaagcaac tcctctttg gccggcttt 
      gaagtcaac tcttcggag cagctgacc actccgacg 
      ccgactgat atcaattcg ttctacttc tacaagacc 
      gagcagttc accgtactc tgggtgctg tttacaatg 
      atcgttctg ggtaattcc gccgtttta gttgccctt 
      ctcgttagc aaaagtcga aaatctaga atgaatttc 
      ttcatctgc caacttgcc attgcggat ttagcggta 
      ggcttaatt agcgtgtcg acggacatt gcttggagg 
      ataacggtg gcttggtat gctggaaac gtcctttgc 
      aaaatcata cgttttctc caagctgtg gtaacatat 
      tcttcgact tatgtgctc gtcgccctg tccatagac 
      cgatacgac gccatcact catcccatg aacttctcc 
      gggagctgg aagcgtgcg agggtttta gtaatgctg 
      gcgtggctt cttagtatt ctgttctcc ttacctacg 
      gttttcctt tttgaggaa aaacaagtc caaagtatg 
      ccgcagtgt tggatagat ctgcaaacg tggcagtgg 
      aaggtttat ataaccctt gtagcccta gtcctcttc 
      gtttttccc gctcttatc ataagtgct tgctacgcg 
      gtcatcgtt cggacgata tggtccaag agcaagctg 
      ctcataccc gtggggcac atccccata aggcagtgc 
      gacgaccat cgtgataga cctcctcga catttccac 
      gaagagcac gatactcgg cgtgctagc tcgcgggga 
      atcattcct cgcgctaaa attaagact gtaaaaatg 
      actctcgtc atcgttttt gtgttcgtg ctatgctgg 
      agtccctac atagtattt gacttgctc caagtgtat 
      ggccacatt ccgaagact cagacaaat attgccata 
      gctacatta attcaaagc ctggcacca ctgaattca 
      gctgccaat cccgtaatt tattgcctc ttctccaca 
      cacttgtgt cgaaattta cgaaagctg ccaccgttc 
      acgtggata tccaactgc ctgtcgttg tgttttccc 
      ggagtccag ggctccgga cactgtctg ggttacggc 
      agcggcgac tccagcggc accgtcacc acctcactg 
      acccattcc acgcgaaga tctggatct gcagcctcc 
      ctgaggcac acaattagg ctccagccc gcccttaac 
      aacactcat aaggtcgcc atctccgtt gtctag
    • >TC013945 RB
      atgatgagt ggagccccg attccggat gtcgggcaa 
      agctggctg aaaagcaac tcctctttg gccggcttt 
      gaagtcaac tcttcggag cagctgacc actccgacg 
      ccgactgat atcaattcg ttctacttc tacaagacc 
      gagcagttc accgtactc tgggtgctg tttacaatg 
      atcgttctg ggtaattcc gccgtttta gttgccctt 
      ctcgttagc aaaagtcga aaatctaga atgaatttc 
      ttcatctgc caacttgcc attgcggat ttagcggta 
      ggcttaatt agcgtgtcg acggacatt gcttggagg 
      ataacggtg gcttggtat gctggaaac gtcctttgc 
      aaaatcata cgttttctc caagctgtg gtaacatat 
      tcttcgact tatgtgctc gtcgccctg tccatagac 
      cgatacgac gccatcact catcccatg aacttctcc 
      gggagctgg aagcgtgcg agggtttta gtaatgctg 
      gcgtggctt cttagtatt ctgttctcc ttacctacg 
      gttttcctt tttgaggaa aaacaagtc caaagtatg 
      ccgcagtgt tggatagat ctgcaaacg tggcagtgg 
      aaggtttat ataaccctt gtagcccta gtcctcttc 
      gtttttccc gctcttatc ataagtgct tgctacgcg 
      gtcatcgtt cggacgata tggtccaag agcaagctg 
      ctcataccc gtggggcac atccccata aggcagtgc 
      gacgaccat cgtgataga cctcctcga catttccac 
      gaagagcac gatactcgg cgtgctagc tcgcgggga 
      atcattcct cgcgctaaa attaagact gtaaaaatg 
      actctcgtc atcgttttt gtgttcgtg ctatgctgg 
      agtccctac atagtattt gacttgctc caagtgtat 
      ggccacatt ccgaagact cagacaaat attgccata 
      gctacatta attcaaagc ctggcacca ctgaattca 
      gctgccaat cccgtaatt tattgcctc ttctccaca 
      cacttgtgt cgaaattta cgatgctcc tcattacta 
      aaacattta aacttcgat ccgtgcaga aagctgcca 
      ccgttcacg tggatatcc aactgcctg tcgttgtgt 
      tttcccgga gtccagggc tccggacac tgtctgggt 
      tacggcagc ggcgactcc agcggcacc gtcaccacc 
      tcactgacc cattccacg cgaagatct ggatctgca 
      gcctccctg aggcacaca attaggctc cagcccgcc 
      cttaacaac actcataag gtcgccatc tccgttgtc 
      tag
    mRNA sequence
    • >TC013945 RA
      tggtaaaac accgacgga gccctagcg ttgattcag 
      tgcgcgatg atgacatta agtgcttaa tgctgtgtg 
      gagcatcga gataacaat gcgtatatg gaaagagga 
      tttgcgctg ctgccacga aaccggcga ctttggttc 
      gaggatgag ctccgtgcc tcacgattc ggttaattg 
      acgaacaaa gcgatgaca gctgataaa aatgactct 
      atagtctgc ttgtaaatc gaccgaatg atgagtgga 
      gccccgatt ccggatgtc gggcaaagc tggctgaaa 
      agcaactcc tctttggcc ggctttgaa gtcaactct 
      tcggagcag ctgaccact ccgacgccg actgatatc 
      aattcgttc tacttctac aagaccgag cagttcacc 
      gtactctgg gtgctgttt acaatgatc gttctgggt 
      aattccgcc gttttagtt gcccttctc gttagcaaa 
      agtcgaaaa tctagaatg aatttcttc atctgccaa 
      cttgccatt gcggattta gcggtaggc ttaattagc 
      gtgtcgacg gacattgct tggaggata acggtggct 
      tggtatgct ggaaacgtc ctttgcaaa atcatacgt 
      tttctccaa gctgtggta acatattct tcgacttat 
      gtgctcgtc gccctgtcc atagaccga tacgacgcc 
      atcactcat cccatgaac ttctccggg agctggaag 
      cgtgcgagg gttttagta atgctggcg tggcttctt 
      agtattctg ttctcctta cctacggtt ttccttttt 
      gaggaaaaa caagtccaa agtatgccg cagtgttgg 
      atagatctg caaacgtgg cagtggaag gtttatata 
      acccttgta gccctagtc ctcttcgtt tttcccgct 
      cttatcata agtgcttgc tacgcggtc atcgttcgg 
      acgatatgg tccaagagc aagctgctc atacccgtg 
      gggcacatc cccataagg cagtgcgac gaccatcgt 
      gatagacct cctcgacat ttccacgaa gagcacgat 
      actcggcgt gctagctcg cggggaatc attcctcgc 
      gctaaaatt aagactgta aaaatgact ctcgtcatc 
      gtttttgtg ttcgtgcta tgctggagt ccctacata 
      gtatttgac ttgctccaa gtgtatggc cacattccg 
      aagactcag acaaatatt gccatagct acattaatt 
      caaagcctg gcaccactg aattcagct gccaatccc 
      gtaatttat tgcctcttc tccacacac ttgtgtcga 
      aatttacga aagctgcca ccgttcacg tggatatcc 
      aactgcctg tcgttgtgt tttcccgga gtccagggc 
      tccggacac tgtctgggt tacggcagc ggcgactcc 
      agcggcacc gtcaccacc tcactgacc cattccacg 
      cgaagatct ggatctgca gcctccctg aggcacaca 
      attaggctc cagcccgcc cttaacaac actcataag 
      gtcgccatc tccgttgtc tagtcacta ggtgtgtta 
      tggttaaac aggagctgg ctgagcccc aggccaaat 
      ccctgctcc attacggaa ctaatcaag gcattttac 
      gtaacttga agcagcgga gtgagaaaa tcacgggcg 
      ccgacgaat tttttaatt tttttcaac aagaacaaa 
      aaaattaaa tcgtctttt tgcgtcaaa ttttgatga 
      attcaagca gtcaaaccc cacaagtga tttacaaca 
      acataaaat atttgtttt ttatgaggt ggaaaattt 
      taaaattcg ggcccatga acaaatggt aagctttgg 
      ttccaatta tgtgatttt tgtatacgt ttttattta 
      ttatttatt aacgatctc ccaattttg ttgatggat 
      taaagtagg ccgtggtat gggccactg ttgccaacc 
      acaatcaga tatcactgc cctacgttt ttttatttt 
      agtttatca taatttact ctttagtag tgtaaataa 
      ccaaagctt acgcttgtc tccttccac gaaaatccc 
      cactaaggc atttcttat ttgcatgaa ccatgacaa 
      taggacaac aataaagct ctgttagtg ctagtttaa 
      aatagaaat caacacacg tccaccaac cctctcttg 
      taaatatgt gtaaatatg aattgagtt tacgtaatg 
      ttgtagcaa tgacacacg tgttcagac gttgtaaat 
      atgtgtaaa aatatactg ttaaacagg gtgtttaca 
      atgttttat gtccagctc cagtattaa gtgttgaat 
      taaagcatt ttaaagtat aacttttct tttcttatt 
      ttgataaat gacatcacg gcacggttg taaataacc 
      cggcgcatc caccatgtt tacgtgttg aatactttt 
      agtttaaaa ataacatga acgagaaat agagatgtt 
      tacagtata cctactcta atgaaaata gagaattat 
      ttattgtat aaataaaac tatttgtta cat
    • >TC013945 RB
      tggtaaaac accgacgga gccctagcg ttgattcag 
      tgcgcgatg atgacatta agtgcttaa tgctgtgtg 
      gagcatcga gataacaat gcgtatatg gaaagagga 
      tttgcgctg ctgccacga aaccggcga ctttggttc 
      gaggatgag ctccgtgcc tcacgattc ggttaattg 
      acgaacaaa gcgatgaca gctgataaa aatgactct 
      atagtctgc ttgtaaatc gaccgaatg atgagtgga 
      gccccgatt ccggatgtc gggcaaagc tggctgaaa 
      agcaactcc tctttggcc ggctttgaa gtcaactct 
      tcggagcag ctgaccact ccgacgccg actgatatc 
      aattcgttc tacttctac aagaccgag cagttcacc 
      gtactctgg gtgctgttt acaatgatc gttctgggt 
      aattccgcc gttttagtt gcccttctc gttagcaaa 
      agtcgaaaa tctagaatg aatttcttc atctgccaa 
      cttgccatt gcggattta gcggtaggc ttaattagc 
      gtgtcgacg gacattgct tggaggata acggtggct 
      tggtatgct ggaaacgtc ctttgcaaa atcatacgt 
      tttctccaa gctgtggta acatattct tcgacttat 
      gtgctcgtc gccctgtcc atagaccga tacgacgcc 
      atcactcat cccatgaac ttctccggg agctggaag 
      cgtgcgagg gttttagta atgctggcg tggcttctt 
      agtattctg ttctcctta cctacggtt ttccttttt 
      gaggaaaaa caagtccaa agtatgccg cagtgttgg 
      atagatctg caaacgtgg cagtggaag gtttatata 
      acccttgta gccctagtc ctcttcgtt tttcccgct 
      cttatcata agtgcttgc tacgcggtc atcgttcgg 
      acgatatgg tccaagagc aagctgctc atacccgtg 
      gggcacatc cccataagg cagtgcgac gaccatcgt 
      gatagacct cctcgacat ttccacgaa gagcacgat 
      actcggcgt gctagctcg cggggaatc attcctcgc 
      gctaaaatt aagactgta aaaatgact ctcgtcatc 
      gtttttgtg ttcgtgcta tgctggagt ccctacata 
      gtatttgac ttgctccaa gtgtatggc cacattccg 
      aagactcag acaaatatt gccatagct acattaatt 
      caaagcctg gcaccactg aattcagct gccaatccc 
      gtaatttat tgcctcttc tccacacac ttgtgtcga 
      aatttacga tgctcctca ttactaaaa catttaaac 
      ttcgatccg tgcagaaag ctgccaccg ttcacgtgg 
      atatccaac tgcctgtcg ttgtgtttt cccggagtc 
      cagggctcc ggacactgt ctgggttac ggcagcggc 
      gactccagc ggcaccgtc accacctca ctgacccat 
      tccacgcga agatctgga tctgcagcc tccctgagg 
      cacacaatt aggctccag cccgccctt aacaacact 
      cataaggtc gccatctcc gttgtctag tcactaggt 
      gtgttatgg ttaaacagg agctggctg agccccagg 
      ccaaatccc tgctccatt acggaacta atcaaggca 
      ttttacgta acttgaagc agcggagtg agaaaatca 
      cgggcgccg acgaatttt ttaattttt ttcaacaag 
      aacaaaaaa attaaatcg tctttttgc gtcaaattt 
      tgatgaatt caagcagtc aaaccccac aagtgattt 
      acaacaaca taaaatatt tgtttttta tgaggtgga 
      aaattttaa aattcgggc ccatgaaca aatggtaag 
      ctttggttc caattatgt gatttttgt atacgtttt 
      tatttatta tttattaac gatctccca attttgttg 
      atggattaa agtaggccg tggtatggg ccactgttg 
      ccaaccaca atcagatat cactgccct acgtttttt 
      tattttagt ttatcataa tttactctt tagtagtgt 
      aaataacca aagcttacg cttgtctcc ttccacgaa 
      aatccccac taaggcatt tcttatttg catgaacca 
      tgacaatag gacaacaat aaagctctg ttagtgcta 
      gtttaaaat agaaatcaa cacacgtcc accaaccct 
      ctcttgtaa atatgtgta aatatgaat tgagtttac 
      gtaatgttg tagcaatga cacacgtgt tcagacgtt 
      gtaaatatg tgtaaaaat atactgtta aacagggtg 
      tttacaatg ttttatgtc cagctccag tattaagtg 
      ttgaattaa agcatttta aagtataac ttttctttt 
      cttattttg ataaatgac atcacggca cggttgtaa 
      ataacccgg cgcatccac catgtttac gtgttgaat 
      acttttagt ttaaaaata acatgaacg agaaataga 
      gatgtttac agtatacct actctaatg aaaatagag 
      aattattta ttgtataaa taaaactat ttgttacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY