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Gene information
General information
TC013944 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2664919..2676443
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013944
    iBeetle screen iB_05528

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature potentially thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature partially ectopicallypresent - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    dorsal ridge row need term irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: dorsal ridge row bristles can be either partially missing or be of irregular size (bristles replaced by setae or vice versa)
    gena triplet seta irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: irregular number of gena triplet bristles. sometimes one or more are missing or duplicated.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013944 RA
      MSDEGKESH KSLPLDLAR TQITTVRTV EGKAPLLMT 
      NQGLRGVRV LTQGTPPNS GQASSNAQT IITTNIVPT 
      VFKPDAART QIASILTTS QPQSSAANI TIQRPTQIT 
      LSTTPVVPC GTNYHVPRG PAVVANLAA PRSNVASVR 
      APMVVTAQT GGQAHAFVR PPRTPSPAQ GTAWLTNTS 
      NGSQIKGAT TVLSSPVRG ASVTGKPQL VSRSQAGLQ 
      NVSTIRPGT TILHSAITI GHSFKPGQN ASGIQGLGN 
      TVTIAQVLP SRTQAVVYS ANTSGQFTA TPRLTVSSS 
      AQNQRQSTA ARPMVTTAR LTIPVNVTQ TGARLVAPQ 
      ATVLTSGAR ITAVSQAST VIGTTPNRI VTTQPNVTI 
      GRLSVAASP AQVSNSNII GQTRTLNLL AVANPSQPR 
      SVHTQQATK VITQPAQGA IHFTPISAP VKTTQTVVT 
      AANRTVNVP AAIVSQRQP AVVSSQTIP IANVYSQQG 
      DNQQGQTPA SVFIPLSSV PRRTSPSPT SAPQTSINT 
      TTSSVTTYS LAASSYFYD GRSFGQQPT SFTALPQST 
      QQIRPTSLN TQVHGIVTN QQMRFNPVM VVDQNRTQQ 
      FPTQSAAEL NSEQSQTVP TKMTSSPRP SILRKRDHE 
      GSPLKAAKN LTPVLTNLT QQPLPSISP PSRPDSRGN 
      GHSSGGSTT ISATSSPGL AEVNEDSMP HAPMNKEEE 
      NVKPPMEMS PRKKPRKQQ LTGSNDIDE HNDDMQFIT 
      DSSLKKEQE DSDGNQSDE PRDGAPEAG QVTTVRKPA 
      SASLLNSYR QTWKATHNH YLRYSDVRP KDERRPTIM 
      DLANQYRVQ EKVNGWKIY HLSTQMEDL AEQEQQVYN 
      QLNELLKNT ESQEELDFE KDINRINEL IKGNLQRIK 
      IINDGMIEA KTQIMKIFD HKGHVTDII NRCASKRNF 
      KKRDKS
    CDS sequence
    • >TC013944 RA
      atgagtgac gagggcaag gagtcccac aagtccttg 
      cctctggat ttggccagg acccaaata accaccgtt 
      cggacagtg gaagggaaa gcccccttg ttgatgacg 
      aatcaaggc ttgcgtgga gtgcgtgtc ctgactcag 
      ggaacgcca ccaaactcg ggccaggct tcttccaac 
      gcccaaaca ataatcaca actaatatt gtccccacc 
      gtgtttaag ccagatgct gcaaggact caaattgcg 
      agtattttg acgacgagt cagccgcaa tcgagtgct 
      gctaacatc actattcaa agacccacc caaatcacc 
      ctttcgaca actccggtg gtaccatgt ggtactaat 
      taccatgtc ccacggggg cccgcagtc gtggcgaat 
      ttggcggcc cctcgcagc aacgtggcg tcggtgcgc 
      gcccccatg gtggtcacg gcgcaaaca ggcggccag 
      gcgcacgca tttgtgagg ccccctcgt actccgagc 
      cccgctcag ggcacggca tggctcacc aacacgtcg 
      aacgggtct cagattaag ggggcaacg actgtgttg 
      agttcacca gtgcggggt gcaagtgtc accgggaag 
      ccccaactt gtctctcgg tcacaagcc gggcttcaa 
      aacgtgtca acaattcgt ccaggaacg acaattttg 
      cattcagct atcacgatt ggacactcg ttcaagcct 
      ggacaaaac gcgtccggg attcaggga ttaggcaat 
      actgtgact atagcacag gtacttccg tcgaggacc 
      caggctgtg gtttatagt gctaatact agtggccag 
      tttacggca acacccaga cttacagtg tcgagttcg 
      gcgcaaaat caaagacag agcactgcg gctagacct 
      atggtaacg acggcacgt ttgacgatt cctgttaat 
      gttacgcaa actggggcg cggttggtg gccccccaa 
      gcgactgtt ttgacttct ggggctagg atcactgct 
      gtgtctcaa gcctcgaca gtgatcggt actacaccg 
      aatagaatt gtgacaacg cagcctaat gttacgata 
      gggcgattg tctgtggct gcaagtccg gcacaagtc 
      tcaaactcc aatattatc ggccagacc cggacactt 
      aatcttctt gctgtagct aatccttcg cagcctaga 
      agtgtgcat acgcagcaa gctactaag gttataacg 
      caacctgct caaggtgcc attcatttt actccaata 
      tcagcccct gtgaagacg acgcaaact gtcgtcacg 
      gcggctaat cgaactgtt aatgttcca gctgcaata 
      gttagtcag aggcaacca gcggttgtt tcttcgcaa 
      actatacca atagctaat gtgtattct caacaaggc 
      gataatcaa caaggacaa actccagcc agtgttttt 
      attccgttg tcgtctgtg cctaggaga acctctcca 
      agtcctaca agtgcaccc caaaccagc atcaacaca 
      acaactagc agcgtaaca acatattcg ctcgccgca 
      agttcgtat ttttacgat ggccgttct ttcggccaa 
      caaccgaca tctttcacc gctcttcca caatccact 
      caacaaata cggccaacg tctctaaac acacaagtc 
      cacggtatt gtgaccaat caacaaatg cgctttaac 
      ccagtcatg gtagtcgac cagaaccgt actcaacaa 
      ttccccacc caatcggcg gcggagcta aattcggaa 
      caatcccaa accgttcca accaaaatg acatcgtca 
      ccacgtccc agcattcta cgaaagcga gaccacgag 
      gggtcgccc ctcaaagcg gccaaaaat ctaactccg 
      gtcctgact aatctcacc caacagccg ctaccgtcg 
      atttcacca ccttcgagg ccggattca cgaggaaat 
      ggacacagt tctggcgga agtacgact atttctgca 
      acgtccagt cccggattg gccgaagtg aacgaggat 
      agcatgccc cacgccccc atgaataag gaggaggag 
      aacgtgaag ccgcccatg gagatgagc ccaaggaaa 
      aaacccaga aagcagcaa ttgactggg agcaatgat 
      attgatgag cataacgat gacatgcag tttataact 
      gacagttcg ctgaagaag gagcaggag gactccgat 
      gggaatcaa agtgatgag ccgagagat ggcgctccg 
      gaggctggg caagtgact acggttcgg aaaccggcc 
      tctgcgagt ctgttgaat agttatcga cagacttgg 
      aaggctaca cacaaccac tatttaagg tattcagat 
      gtgaggcct aaagacgag cggaggcct accatcatg 
      gacttggcc aatcaatac agagtgcaa gaaaaagtg 
      aacggatgg aagatttat catctttcg actcaaatg 
      gaggatttg gccgagcaa gagcaacaa gtctacaac 
      cagttgaat gagctgctt aaaaacacc gaatctcaa 
      gaggagcta gattttgag aaggatata aaccgaatc 
      aacgaactc atcaagggt aatttacag agaattaaa 
      attatcaac gatggaatg attgaggcg aagacccaa 
      attatgaag atcttcgac cataagggc catgtgacc 
      gatataatc aatagatgt gccagtaaa cgtaacttc 
      aagaaacga gataaatcg taa
    mRNA sequence
    • >TC013944 RA
      gagcaatgg cgtcagttc ggaatttgc gttttgtgg 
      tgttgttgt agtctatta ttgtagatt ttttaagtg 
      caaacctta gtcttaagt ttaaccaaa aaaccatgt 
      tattttgtt ccgaaaaat tgccaaaaa gctgccaag 
      gtttcattt ttacacttt gcaaaacct cctaattac 
      cctctgttc agttttaat gcagatgcg tttaacgta 
      acaaataaa gttttttat gggtaaatt taaaacctg 
      cgaaatatg agtgacgag ggcaaggag tcccacaag 
      tccttgcct ctggatttg gccaggacc caaataacc 
      accgttcgg acagtggaa gggaaagcc cccttgttg 
      atgacgaat caaggcttg cgtggagtg cgtgtcctg 
      actcaggga acgccacca aactcgggc caggcttct 
      tccaacgcc caaacaata atcacaact aatattgtc 
      cccaccgtg tttaagcca gatgctgca aggactcaa 
      attgcgagt attttgacg acgagtcag ccgcaatcg 
      agtgctgct aacatcact attcaaaga cccacccaa 
      atcaccctt tcgacaact ccggtggta ccatgtggt 
      actaattac catgtccca cgggggccc gcagtcgtg 
      gcgaatttg gcggcccct cgcagcaac gtggcgtcg 
      gtgcgcgcc cccatggtg gtcacggcg caaacaggc 
      ggccaggcg cacgcattt gtgaggccc cctcgtact 
      ccgagcccc gctcagggc acggcatgg ctcaccaac 
      acgtcgaac gggtctcag attaagggg gcaacgact 
      gtgttgagt tcaccagtg cggggtgca agtgtcacc 
      gggaagccc caacttgtc tctcggtca caagccggg 
      cttcaaaac gtgtcaaca attcgtcca ggaacgaca 
      attttgcat tcagctatc acgattgga cactcgttc 
      aagcctgga caaaacgcg tccgggatt cagggatta 
      ggcaatact gtgactata gcacaggta cttccgtcg 
      aggacccag gctgtggtt tatagtgct aatactagt 
      ggccagttt acggcaaca cccagactt acagtgtcg 
      agttcggcg caaaatcaa agacagagc actgcggct 
      agacctatg gtaacgacg gcacgtttg acgattcct 
      gttaatgtt acgcaaact ggggcgcgg ttggtggcc 
      ccccaagcg actgttttg acttctggg gctaggatc 
      actgctgtg tctcaagcc tcgacagtg atcggtact 
      acaccgaat agaattgtg acaacgcag cctaatgtt 
      acgataggg cgattgtct gtggctgca agtccggca 
      caagtctca aactccaat attatcggc cagacccgg 
      acacttaat cttcttgct gtagctaat ccttcgcag 
      cctagaagt gtgcatacg cagcaagct actaaggtt 
      ataacgcaa cctgctcaa ggtgccatt cattttact 
      ccaatatca gcccctgtg aagacgacg caaactgtc 
      gtcacggcg gctaatcga actgttaat gttccagct 
      gcaatagtt agtcagagg caaccagcg gttgtttct 
      tcgcaaact ataccaata gctaatgtg tattctcaa 
      caaggcgat aatcaacaa ggacaaact ccagccagt 
      gtttttatt ccgttgtcg tctgtgcct aggagaacc 
      tctccaagt cctacaagt gcaccccaa accagcatc 
      aacacaaca actagcagc gtaacaaca tattcgctc 
      gccgcaagt tcgtatttt tacgatggc cgttctttc 
      ggccaacaa ccgacatct ttcaccgct cttccacaa 
      tccactcaa caaatacgg ccaacgtct ctaaacaca 
      caagtccac ggtattgtg accaatcaa caaatgcgc 
      tttaaccca gtcatggta gtcgaccag aaccgtact 
      caacaattc cccacccaa tcggcggcg gagctaaat 
      tcggaacaa tcccaaacc gttccaacc aaaatgaca 
      tcgtcacca cgtcccagc attctacga aagcgagac 
      cacgagggg tcgcccctc aaagcggcc aaaaatcta 
      actccggtc ctgactaat ctcacccaa cagccgcta 
      ccgtcgatt tcaccacct tcgaggccg gattcacga 
      ggaaatgga cacagttct ggcggaagt acgactatt 
      tctgcaacg tccagtccc ggattggcc gaagtgaac 
      gaggatagc atgccccac gcccccatg aataaggag 
      gaggagaac gtgaagccg cccatggag atgagccca 
      aggaaaaaa cccagaaag cagcaattg actgggagc 
      aatgatatt gatgagcat aacgatgac atgcagttt 
      ataactgac agttcgctg aagaaggag caggaggac 
      tccgatggg aatcaaagt gatgagccg agagatggc 
      gctccggag gctgggcaa gtgactacg gttcggaaa 
      ccggcctct gcgagtctg ttgaatagt tatcgacag 
      acttggaag gctacacac aaccactat ttaaggtat 
      tcagatgtg aggcctaaa gacgagcgg aggcctacc 
      atcatggac ttggccaat caatacaga gtgcaagaa 
      aaagtgaac ggatggaag atttatcat ctttcgact 
      caaatggag gatttggcc gagcaagag caacaagtc 
      tacaaccag ttgaatgag ctgcttaaa aacaccgaa 
      tctcaagag gagctagat tttgagaag gatataaac 
      cgaatcaac gaactcatc aagggtaat ttacagaga 
      attaaaatt atcaacgat ggaatgatt gaggcgaag 
      acccaaatt atgaagatc ttcgaccat aagggccat 
      gtgaccgat ataatcaat agatgtgcc agtaaacgt 
      aacttcaag aaacgagat aaatcgtaa attaagttt 
      gttgtgatt ttgtgtttt ttgatcttg tgcttttat 
      aattatcga cattaggtc aaagatcga aagaaaaag 
      tagttttat acaattcgc ttgtatatt tttctgagt 
      tttcattgt atttattta tttgatgtt gatattttg 
      aattataaa aagatctaa aagtggaca aatggtttt 
      gtaataaat aaaagtgtg caggttttg tacacggct 
      tgtatttat attattgca tccattggt ggatgagac 
      aacttttcc agtgaacct actgttgat gtttgtaat 
      aaagttgtt ttaaatctc gacagtcta agttttcct 
      tgtccttgt gtgcagata gattatatt attataaaa 
      ttgtttctt aaataaatg gtttctaaa gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY