General information | |||
TC013942 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2633509..2652673 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC013942 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05527 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC013942 RA
MTSNARAFT QKLSKHNAA DVRRNVACH KGGVISPSS SSSWANDVV DPIDFEEFL SQYQNLIDR DPLRSILDV PQGDVEVDV IERPIRTLH PILPEEKLE TLPPHVQAC VECYTSNWK VVRYNHRNL SSSVATKAN PSKSLQPSP KQEFEVDFQ PNTFEATSE SDLTSISSS SRQSIASLV SSSSCGDTL TPRNSWASL DLRHSAGDP LIPEILEQN APEALDQLN ESRRQTERQ DGLFALCSN LDKEAGEPI ERRLPAAPP AEPLGHRVL VKCHQLTLD LEVEPLFAS MAIYDCKER KKLSETFYF DLNPEGLKR MLGGHVPYS DTSTLARGC IFNITNPSP DLFLVVRLE KVLQGDLNE CVEPYMKEE RNRDKLKAN AVAVCERLG KYRQAFAWT GIYLMNVIN GGNSLERES DRDSFSSNS ATNSLDRKL GSSSLEQLR RRATDMGSL TRRGSLERR TDKRRSWSP DHLASTLET FRPITLTVS SFFKQEGEK LRDEDLYKC LHELKKPTT ILKKLKCIP ATLKLEIAP CPGEYKNCL TTELIKLYP YPDDKGRPI KELLEFPIK ELLVPHYNY RNLLFVSPK ELNFSNRAG SARNLAVRV QLMAGECEN QALCNIFGK SSCPEMTNE AYSVVTYHS KNPFFYDEI KIKLPAALS DNHHLLFTF YHISCQKKV EQSSVDSPV GYTWLPLIR DGRLISGEF CLPVMLETP PKNYSYIPP DVYLPGTKW LDNHKGLFT VHLDSVSSV HTHDPAVER FLAAYDYVH TGVIPPRLG EAGLENELR AALLGLAGA KPETIVKNL PLIFDSVLE LLVQPPRIS GHTLNIGQT TFEAFCLLL ENISKLDLP TDQHNRNAL LATYIHYQC NLPHPLSTS NHSSPDWEE GVPLTRSNS NPDLEVAHL QQSRGLDRA ASMRVPNND QISSSINTC QKIVHHEIA LQWVVSSGR IKDLAMQNA WFLFELTTK SMVEHLAHT RSLDAPRKI RFNEQFLDD ILSLVHTIT AEIISHSTS DIKRAHMLN AALAFFFFD LLSFCDRGS VFNAIRYYN KQLQAKIMS MQDAAVLVE LKLECARIV CSHEHYVAL NLPFATPFM FDESKFVFI GASTKSRKS EYSFAELSP EYRQHHYLT GIVLTDVAS ILLDVSKPN LHVKAVDTI KSLLSWHDS DPRYSTPEA RRRVAALYL PLLTIAMDV LPLLHRWSS DKPDRYSNE DETTNINQT VALAIAGKV PPVTCDNLQ STRKSGISA EVTRNLLTC VLWVLKNVE RESLSQWLG ELTSTRLAT LLHLLDACT SCFEYRPRR RAPPPSGYA HAQVSQDGL SLLEDVILG QGSAREMMQ RRKGGIQNP GSGEVKLRW RKEQMAYRP STDTVDRPK EDLFPDVHL EGHFATEAS FIILDTLER CVATVAQWD SQQYLVGLA LTVLLHALS KNQSTTILP HLFASQRNL VFKFHSALF DEESTHCAD LCLLLLKHC GSQIASVRS QAAASLYLL MRQTFQIGN NFARVKMQV TMSLSSLVG TSSSFSDES LRRSLKTIL EYGERDTEL QETTFPEQV RDLVFNLHM ILSDTVKMK EFQEDPEML LDLMYRIAK GYQNSPDLR LTWLENMAQ KHMERSNHC EAGMCLVHS AALVAEYLV MSESSPHLP AGAAALERV SPNVLEESA VSDDVLSPE KEGGCLGSH FTEAGLVGL LEQAASSFH VAAMYEPMN DIYRVLIPI AENNRDFKK LANIHGKLH DAYTRIDQL QGKRMFGTY FRVGFYGSK FGDLDREEF IYKEPTLTK LPEIFSRLE NFYAERFGP ENVIIIKDS NIVDISALD PDKAYIQIT YVEPYFEQY ELRYRQTHF DRNFNIKRF VYATPFTMT GKAHGELRD QYKRKTILT TAVHFPYVK TRIQVVNRV QITLTPIEV AIEDIQKKT TELAAAIQQ EPSDTKILQ MVLQGCIGT TVNQGPLEM ALTFLPSDG KALTKHQNK LRLCFKDFC KKCSDALKK NRNLIGPDQ REYQRELDR NFKRLTEKL QPLVNPQNI TIVSASPHK YPNNESTYL RW
- >TC013942 RA
atgacttcg aacgccagg gcgttcact caaaaatta tcaaaacac aatgccgcc gatgtccgt cgaaatgta gcgtgtcat aaaggcggc gttatttcg ccgtcgtcg tcctcaagc tgggctaac gacgtcgtg gaccccatc gatttcgag gagtttcta agccagtat cagaaccta atcgacagg gaccccttg cgatcaatt ctagacgtg cctcagggg gatgtggag gttgacgtt attgaacgt cctatccgc accttgcac cccatccta ccggaagaa aaacttgag actctgcct cctcatgtg caagcctgt gtggagtgt tacacctca aactggaaa gttgtcaga tacaaccat aggaatctc agtagcagt gtcgccacc aaagccaac ccttcgaaa agcctccag ccgagcccc aaacaagaa tttgaagtt gatttccaa cccaacact tttgaagcc accagtgaa tcagaccta acgtcgatt tcaagcagt agtcggcaa agcattgct tcgttggtg tccagttca tcgtgtggt gatactttg actcctcga aactcatgg gcgtctctg gacttacgc cactccgcc ggggatcca ctcattcca gaaatattg gaacagaat gcgccagaa gctttggat cagttgaat gaaagtcga cgccagact gaacgacaa gatgggttg tttgccttg tgttcgaat ctggataag gaggctggc gagcccatt gaaaggcgg ttgccggcg gcgcccccg gccgagcct ttggggcac agggtcctc gtcaagtgc caccagttg acgttggac ttggaagtt gagcctttg tttgcatct atggcgatt tacgattgt aaagaacgc aaaaaattg tcggaaacc ttctacttt gatttaaat ccggaaggt ttgaaacga atgttagga ggtcatgtg ccttatagc gacaccagt accctcgca aggggttgc attttcaac attactaat ccgtctcct gatttattt ttagtcgtg aggctagag aaagttcta caaggtgat ttgaatgaa tgtgtggaa ccttatatg aaggaagaa agaaatcgt gataaactt aaggcaaac gccgtcgcc gtttgcgag cgtttgggg aagtacaga caggcgttt gcttggacg ggaatctat ttaatgaat gtgataaac gggggaaac tcgttagaa cgtgaatcg gacagggat tcgttcagt tcaaactcg gccactaac agtttagat cgcaaattg gggagcagt agcttggaa cagttacgc agacgagcc acagacatg ggaagttta acacgaagg ggttcactt gaacgcaga acggacaaa agacgatca tggtcacct gaccattta gcgtccact ttggaaact ttccgaccg ataacttta accgtgtct tcatttttt aaacaagaa ggtgagaaa ttacgcgac gaggatctc tacaagtgc cttcacgaa ctcaaaaaa cccacaaca atactgaaa aaattgaag tgcattccg gctactttg aaattagaa atagcacct tgtcccgga gaatacaaa aattgccta actacggaa ttgataaaa ttgtatccg tatcccgat gataagggg agaccgatc aaagaatta ctcgaattt ccgattaag gaattgcta gtgcctcac tacaactat cgcaatttg ttatttgtg agtccgaaa gaactgaat ttttcaaac agggccgga tcggctcgt aatttggct gtgagagtg caattaatg gctggcgag tgtgaaaat caggctttg tgcaatatt tttggcaag tctagttgt ccagaaatg accaatgag gcctacagt gtggtgact tatcacagt aaaaatccc ttcttttac gatgaaatt aaaataaaa ctgccggcc gcgctatcc gataatcac catttgtta ttcaccttt tatcacatt tcgtgtcag aagaaggtg gagcagtcg tcagtggat tccccagtg ggatataca tggctgcct ctaatccgt gatggtcga ctgataagt ggcgagttt tgtctcccg gttatgttg gaaacgccg cctaaaaat tacagttat attcccccc gatgtgtac ctgcctggt accaaatgg ttggataac cacaaaggg ttatttaca gttcatcta gattcggtt tcttcagtc cacacacat gacccagct gttgaacga tttctcgca gcttatgat tacgtccat accggggtt ataccccca agattaggc gaagccggc ttagaaaat gaattaaga gcggctctg ttgggtcta gctggagct aaacccgaa acaatcgtc aaaaatttg ccgttaatt ttcgacagc gtgttagaa cttttggta caaccgcca cgaatctct ggtcatact ttaaacatt ggccaaacc acatttgag gctttttgc ttactgttg gagaacatt tcgaagctt gatttaccg actgaccaa cacaaccgg aacgctctc ctcgcaacg tacatccat taccaatgc aatctcccc catccactt tcgacgagt aatcacagt tcccccgac tgggaggaa ggggtgcct ctaactcga agtaattca aacccggac ttggaagtg gcccacttg caacaatca cgtggttta gatcgggct gcaagtatg cgagtccct aataatgat caaatcagt tcgagcata aacacttgt cagaagata gtccatcac gagatcgct cttcaatgg gtggtttcg agcggccgt attaaagat ttagccatg caaaacgcc tggttcttg tttgaattg acaacgaaa agtatggtt gaacacttg gcccatacg cggagttta gatgcgcct cgaaaaatc cgttttaat gaacaattc ctcgatgat attctctca ttggtgcac acgataacg gctgagatt atctcgcat tcgactagt gatattaaa agggcgcat atgctgaac gccgccctt gctttcttc ttcttcgat ttgctgtcg ttttgtgac cgaggctct gttttcaac gcaattcgc tactataat aaacaacta caagcgaag ataatgtca atgcaagat gccgcggtt ttagttgag ttgaaacta gagtgtgct aggattgtg tgcagtcat gagcattac gttgctttg aatttgccc ttcgcaact ccgtttatg ttcgacgag tcaaaattc gtttttatc ggggcctcc accaagtca agaaagagt gagtactct tttgcggag ttgtcgcca gagtatcgg caacatcat tacttgact gggattgtt ctaacggac gttgcgagt attttactc gatgtcagt aaaccgaat ttgcacgtt aaagctgtg gatacgata aaatcgctt ttgtcgtgg cacgacagc gatccgaga tattcaaca cctgaggct cgaagacgt gttgcagct ctctatttg ccactgttg accattgca atggatgtg ttgccactg ttgcatcgc tggtcgtcg gataaaccc gatcggtac tcgaacgag gacgaaact acgaatatc aaccaaact gtggctttg gcgattgcc gggaaagtc ccgccagtc acttgcgat aatcttcaa tcgactcgc aagtcggga attagcgcc gaggtgacc cgcaacctc ctcacttgc gtcctctgg gttctcaaa aacgtcgaa cgtgagtcg ctctctcaa tggttgggt gaattgacc tccacacgt cttgcaaca ctcctccac ctcctcgac gcctgtaca agttgtttt gagtatcga ccacgtcgt cgggcgcct ccaccatcg ggctacgcc cacgcccaa gtgtctcag gacggttta tcgctcttg gaagatgtg attttgggt cagggatcg gcgagagaa atgatgcaa agacgcaaa ggtggtata caaaatccc ggctcaggt gaagtcaag ttgagatgg aggaaggaa caaatggcc tacagaccc tcaactgat actgtcgat agaccaaag gaggatttg ttccctgat gtgcatttg gagggacat tttgccacg gaagcctcg tttattatt ttggatacg ttggagagg tgtgtggcc acggttgca cagtgggat tcgcaacag tacctagtt ggattggcc ttgacggtg cttttgcat gccttgagt aaaaatcag agcacgacg attttgccg catttgttc gcatcgcag agaaatttg gtgtttaag tttcacagt gctctcttt gacgaggaa agcacccac tgtgccgac ctctgcctc ctactgcta aaacactgt gggtctcaa atcgcctct gtgcgttcg caagccgcc gcctctttg tatttatta atgagacaa accttccaa atcggaaac aatttcgca cgcgtcaaa atgcaagtt acgatgtct ctcagtagt ctcgtgggc acttcgtcg tcgttcagt gacgaatct ctccgccgc tccctcaaa accattctc gaatacggt gaacgagac accgagttg caagaaacc acatttccc gaacaagtc cgtgatttg gttttcaac ctacacatg attttgtcc gatactgtg aaaatgaaa gaatttcaa gaagacccc gagatgctt ctcgaccta atgtatcgc attgcaaaa gggtatcaa aactcgccc gatttgagg ctgacttgg cttgaaaac atggcccag aagcacatg gaaaggagt aatcattgt gaagctggg atgtgtctt gtgcattcg gctgctcta gtcgctgaa tatcttgtt atgtccgaa tcgtcgccc catttgccg gcaggtgcg gcggctttg gagcgagtt agccccaat gtgttggag gaaagtgcg gtttcagat gatgttttg agtcctgag aaggaaggg ggctgtttg gggtcgcat ttcactgaa gctgggttg gtggggctg ctggaacaa gccgcgagt agttttcat gtggcggcg atgtacgaa ccgatgaat gatatctac agggtgctg ataccaatc gcggaaaat aatcgggat tttaagaag ttggccaat attcatggg aaattacac gatgcatac acgcgtatt gatcaatta caaggaaaa agaatgttc gggacttat tttagagtc ggtttttac ggctcaaag tttggtgat ttagacagg gaggagttt atttataaa gaaccaact ttaaccaaa cttcccgaa atatttagc cggcttgaa aatttttat gccgaaagg tttggccct gaaaacgtc attattatc aaagattca aacattgtt gatatcagt gcgttagac ccagacaag gcttacatt caaatcact tacgttgag ccttatttt gaacaatac gaactgcga tatcgtcaa actcatttc gataggaat ttcaacatc aagcgtttc gtttacgcc actccgttc acaatgacc ggcaaagcg cacggcgaa ctgcgcgac caatacaaa cgcaaaaca atcctaaca actgctgtg cacttccct tacgttaaa acccgaatc caagttgta aatcgggta caaatcaca ctaacaccg atcgaggtg gcgattgag gatattcag aagaaaacc accgaatta gcagctgcc attcagcaa gaaccctcc gacacgaaa atattgcaa atggttttg cagggttgc attggcaca acggttaac caaggaccg cttgaaatg gctttaaca tttttaccg agcgatggg aaagcttta accaaacac caaaacaaa ctaaggctg tgctttaaa gatttttgt aaaaagtgt tcggacgct ttgaagaaa aaccgtaat ttgatcgga cctgatcaa agagagtac caaagagag cttgatagg aattttaaa cggcttact gaaaaattg caaccgctt gttaatccg caaaatatt acgattgtt tcggcaagc ccgcataaa taccccaat aatgaatca acttattta aggtggtag
- >TC013942 RA
tgacattta ttagaagaa aatgacttc gaacgccag ggcgttcac tcaaaaatt atcaaaaca caatgccgc cgatgtccg tcgaaatgt agcgtgtca taaaggcgg cgttatttc gccgtcgtc gtcctcaag ctgggctaa cgacgtcgt ggaccccat cgatttcga ggagtttct aagccagta tcagaacct aatcgacag ggacccctt gcgatcaat tctagacgt gcctcaggg ggatgtgga ggttgacgt tattgaacg tcctatccg caccttgca ccccatcct accggaaga aaaacttga gactctgcc tcctcatgt gcaagcctg tgtggagtg ttacacctc aaactggaa agttgtcag atacaacca taggaatct cagtagcag tgtcgccac caaagccaa cccttcgaa aagcctcca gccgagccc caaacaaga atttgaagt tgatttcca acccaacac ttttgaagc caccagtga atcagacct aacgtcgat ttcaagcag tagtcggca aagcattgc ttcgttggt gtccagttc atcgtgtgg tgatacttt gactcctcg aaactcatg ggcgtctct ggacttacg ccactccgc cggggatcc actcattcc agaaatatt ggaacagaa tgcgccaga agctttgga tcagttgaa tgaaagtcg acgccagac tgaacgaca agatgggtt gtttgcctt gtgttcgaa tctggataa ggaggctgg cgagcccat tgaaaggcg gttgccggc ggcgccccc ggccgagcc tttggggca cagggtcct cgtcaagtg ccaccagtt gacgttgga cttggaagt tgagccttt gtttgcatc tatggcgat ttacgattg taaagaacg caaaaaatt gtcggaaac cttctactt tgatttaaa tccggaagg tttgaaacg aatgttagg aggtcatgt gccttatag cgacaccag taccctcgc aaggggttg cattttcaa cattactaa tccgtctcc tgatttatt tttagtcgt gaggctaga gaaagttct acaaggtga tttgaatga atgtgtgga accttatat gaaggaaga aagaaatcg tgataaact taaggcaaa cgccgtcgc cgtttgcga gcgtttggg gaagtacag acaggcgtt tgcttggac gggaatcta tttaatgaa tgtgataaa cgggggaaa ctcgttaga acgtgaatc ggacaggga ttcgttcag ttcaaactc ggccactaa cagtttaga tcgcaaatt ggggagcag tagcttgga acagttacg cagacgagc cacagacat gggaagttt aacacgaag gggttcact tgaacgcag aacggacaa aagacgatc atggtcacc tgaccattt agcgtccac tttggaaac tttccgacc gataacttt aaccgtgtc ttcattttt taaacaaga aggtgagaa attacgcga cgaggatct ctacaagtg ccttcacga actcaaaaa acccacaac aatactgaa aaaattgaa gtgcattcc ggctacttt gaaattaga aatagcacc ttgtcccgg agaatacaa aaattgcct aactacgga attgataaa attgtatcc gtatcccga tgataaggg gagaccgat caaagaatt actcgaatt tccgattaa ggaattgct agtgcctca ctacaacta tcgcaattt gttatttgt gagtccgaa agaactgaa tttttcaaa cagggccgg atcggctcg taatttggc tgtgagagt gcaattaat ggctggcga gtgtgaaaa tcaggcttt gtgcaatat ttttggcaa gtctagttg tccagaaat gaccaatga ggcctacag tgtggtgac ttatcacag taaaaatcc cttctttta cgatgaaat taaaataaa actgccggc cgcgctatc cgataatca ccatttgtt attcacctt ttatcacat ttcgtgtca gaagaaggt ggagcagtc gtcagtgga ttccccagt gggatatac atggctgcc tctaatccg tgatggtcg actgataag tggcgagtt ttgtctccc ggttatgtt ggaaacgcc gcctaaaaa ttacagtta tattccccc cgatgtgta cctgcctgg taccaaatg gttggataa ccacaaagg gttatttac agttcatct agattcggt ttcttcagt ccacacaca tgacccagc tgttgaacg atttctcgc agcttatga ttacgtcca taccggggt tataccccc aagattagg cgaagccgg cttagaaaa tgaattaag agcggctct gttgggtct agctggagc taaacccga aacaatcgt caaaaattt gccgttaat tttcgacag cgtgttaga acttttggt acaaccgcc acgaatctc tggtcatac tttaaacat tggccaaac cacatttga ggctttttg cttactgtt ggagaacat ttcgaagct tgatttacc gactgacca acacaaccg gaacgctct cctcgcaac gtacatcca ttaccaatg caatctccc ccatccact ttcgacgag taatcacag ttcccccga ctgggagga aggggtgcc tctaactcg aagtaattc aaacccgga cttggaagt ggcccactt gcaacaatc acgtggttt agatcgggc tgcaagtat gcgagtccc taataatga tcaaatcag ttcgagcat aaacacttg tcagaagat agtccatca cgagatcgc tcttcaatg ggtggtttc gagcggccg tattaaaga tttagccat gcaaaacgc ctggttctt gtttgaatt gacaacgaa aagtatggt tgaacactt ggcccatac gcggagttt agatgcgcc tcgaaaaat ccgttttaa tgaacaatt cctcgatga tattctctc attggtgca cacgataac ggctgagat tatctcgca ttcgactag tgatattaa aagggcgca tatgctgaa cgccgccct tgctttctt cttcttcga tttgctgtc gttttgtga ccgaggctc tgttttcaa cgcaattcg ctactataa taaacaact acaagcgaa gataatgtc aatgcaaga tgccgcggt tttagttga gttgaaact agagtgtgc taggattgt gtgcagtca tgagcatta cgttgcttt gaatttgcc cttcgcaac tccgtttat gttcgacga gtcaaaatt cgtttttat cggggcctc caccaagtc aagaaagag tgagtactc ttttgcgga gttgtcgcc agagtatcg gcaacatca ttacttgac tgggattgt tctaacgga cgttgcgag tattttact cgatgtcag taaaccgaa tttgcacgt taaagctgt ggatacgat aaaatcgct tttgtcgtg gcacgacag cgatccgag atattcaac acctgaggc tcgaagacg tgttgcagc tctctattt gccactgtt gaccattgc aatggatgt gttgccact gttgcatcg ctggtcgtc ggataaacc cgatcggta ctcgaacga ggacgaaac tacgaatat caaccaaac tgtggcttt ggcgattgc cgggaaagt cccgccagt cacttgcga taatcttca atcgactcg caagtcggg aattagcgc cgaggtgac ccgcaacct cctcacttg cgtcctctg ggttctcaa aaacgtcga acgtgagtc gctctctca atggttggg tgaattgac ctccacacg tcttgcaac actcctcca cctcctcga cgcctgtac aagttgttt tgagtatcg accacgtcg tcgggcgcc tccaccatc gggctacgc ccacgccca agtgtctca ggacggttt atcgctctt ggaagatgt gattttggg tcagggatc ggcgagaga aatgatgca aagacgcaa aggtggtat acaaaatcc cggctcagg tgaagtcaa gttgagatg gaggaagga acaaatggc ctacagacc ctcaactga tactgtcga tagaccaaa ggaggattt gttccctga tgtgcattt ggagggaca ttttgccac ggaagcctc gtttattat tttggatac gttggagag gtgtgtggc cacggttgc acagtggga ttcgcaaca gtacctagt tggattggc cttgacggt gcttttgca tgccttgag taaaaatca gagcacgac gattttgcc gcatttgtt cgcatcgca gagaaattt ggtgtttaa gtttcacag tgctctctt tgacgagga aagcaccca ctgtgccga cctctgcct cctactgct aaaacactg tgggtctca aatcgcctc tgtgcgttc gcaagccgc cgcctcttt gtatttatt aatgagaca aaccttcca aatcggaaa caatttcgc acgcgtcaa aatgcaagt tacgatgtc tctcagtag tctcgtggg cacttcgtc gtcgttcag tgacgaatc tctccgccg ctccctcaa aaccattct cgaatacgg tgaacgaga caccgagtt gcaagaaac cacatttcc cgaacaagt ccgtgattt ggttttcaa cctacacat gattttgtc cgatactgt gaaaatgaa agaatttca agaagaccc cgagatgct tctcgacct aatgtatcg cattgcaaa agggtatca aaactcgcc cgatttgag gctgacttg gcttgaaaa catggccca gaagcacat ggaaaggag taatcattg tgaagctgg gatgtgtct tgtgcattc ggctgctct agtcgctga atatcttgt tatgtccga atcgtcgcc ccatttgcc ggcaggtgc ggcggcttt ggagcgagt tagccccaa tgtgttgga ggaaagtgc ggtttcaga tgatgtttt gagtcctga gaaggaagg gggctgttt ggggtcgca tttcactga agctgggtt ggtggggct gctggaaca agccgcgag tagttttca tgtggcggc gatgtacga accgatgaa tgatatcta cagggtgct gataccaat cgcggaaaa taatcggga ttttaagaa gttggccaa tattcatgg gaaattaca cgatgcata cacgcgtat tgatcaatt acaaggaaa aagaatgtt cgggactta ttttagagt cggttttta cggctcaaa gtttggtga tttagacag ggaggagtt tatttataa agaaccaac tttaaccaa acttcccga aatatttag ccggcttga aaattttta tgccgaaag gtttggccc tgaaaacgt cattattat caaagattc aaacattgt tgatatcag tgcgttaga cccagacaa ggcttacat tcaaatcac ttacgttga gccttattt tgaacaata cgaactgcg atatcgtca aactcattt cgataggaa tttcaacat caagcgttt cgtttacgc cactccgtt cacaatgac cggcaaagc gcacggcga actgcgcga ccaatacaa acgcaaaac aatcctaac aactgctgt gcacttccc ttacgttaa aacccgaat ccaagttgt aaatcgggt acaaatcac actaacacc gatcgaggt ggcgattga ggatattca gaagaaaac caccgaatt agcagctgc cattcagca agaaccctc cgacacgaa aatattgca aatggtttt gcagggttg cattggcac aacggttaa ccaaggacc gcttgaaat ggctttaac atttttacc gagcgatgg gaaagcttt aaccaaaca ccaaaacaa actaaggct gtgctttaa agatttttg taaaaagtg ttcggacgc tttgaagaa aaaccgtaa tttgatcgg acctgatca aagagagta ccaaagaga gcttgatag gaattttaa acggcttac tgaaaaatt gcaaccgct tgttaatcc gcaaaatat tacgattgt ttcggcaag cccgcataa ataccccaa taatgaatc aacttattt aaggtggta ggacgaaga cgccgatta ttgggttaa aaccaacat ttattttag taagttaag aaccaaatg tacaacaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
