| General information | |||
| TC013939 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2615339..2622016 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013939 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05526 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
- >TC013939 RA
MMEFAQPPP NMAMPPPGM GQQGMNMPP PSMGGPGGY QHNKPPFRP FMNMQRPLP GPLGNMTLE DFDGKRLRK SVMRKTVDY NSSIIKELQ SRVWQRDYR DRRALQPDV MYYPDMMPP PSYQDNPIN CVTTRFVKT ATNKMRCPI FCMAWTPEG RRLVTGASS GEFTLWNGL TFNFETILQ AHDSPVRTM VWSHNDSWM VTGDHAGYV KYWQSNMNN VKMFQAHKE ALRGISFSP SDTKFVTCS DDGTLRIWD FFRYQEERI LRGHGADVK CVHWHPQKG LIVSGSKDN QQPIKLWDP KTGQSLATL HAHKSTVMD LKWNENGNW LITASRDHL LKLFDLRNL NQEVQTFRG HKKEASSVA WHPIHEGLF SSGGSDGSI MFWHVGADK EVGAIEQAH DSIVWTLAW HPLGHILCS GSNDHTSKF WTRNRPGDQ MRDKYNLNT LPAGIAGLE DLDMGEEPS IIPGMGPDD KVELTSLSS DGNTAIPGL DLDLLTTTN EEKTKIKKV PFSKPIPRN FQAQWNETY EDAAGTINE VISQIVENT PGVVPLQKI APSAIIIYG KLIPVEPGS KLEQVIAEG PEALQKYID SGEIEELHD VMPIGDDED YLNDYDSNT DSPGGTTTN IPSLMQTPA APPITQDTN LTRALDSDM RSVDQDMRS FSSYGRDND MRDPDYRSV VNRELNFQP PPRDYDIGD EDYRQPFRN DPDFRHYDD EEGYDEYYD EARNWPRSN GNYGQFGGR NQNFGGNHP NFRGQFEGW SGHSDFRGR GAKRGARGQ RNPRSRPRG GSRGNSRGG APNRGQSSS RGRF
- >TC013939 RA
atgatggag tttgcacaa ccgcccccc aacatggcc atgcctcct ccgggcatg gggcagcag ggcatgaac atgccacca cctagcatg ggcggacct ggagggtac caacacaac aagcctccg tttcgcccc ttcatgaac atgcaaagg ccgctacct gggcccctg ggcaacatg accctcgaa gacttcgac gggaagcgg ctgaggaag tcggtaatg aggaaaact gtagactac aattcttca attataaag gaattacaa tcaagagtg tggcagagg gattacagg gacaggaga gccttgcaa cctgatgtg atgtattac ccagacatg atgcctcct ccgagttac caagataac cccattaac tgtgtcacc actaggttt gttaaaact gcaacaaat aagatgaga tgtcccatt ttttgtatg gcatggacg ccagaagga cggagactt gtcacaggc gccagctct ggggagttt acactctgg aatggcctc accttcaat ttcgaaact atcttacaa gctcatgac agccccgta agaacgatg gtctggagc cacaacgat agttggatg gtgacaggc gaccatgcg ggttatgtg aaatattgg cagtcaaat atgaacaat gtgaagatg ttccaggcc cataaagaa gctcttaga ggcataagc ttcagcccc tcggatacc aagtttgta acgtgctca gacgatggt actctgcga atttgggac tttttccga taccaagag gagcggatt ctgcgaggt cacggggct gatgtaaag tgcgtgcac tggcacccc caaaagggg cttatcgtt tccggtagc aaagacaat cagcagccg attaagctc tgggacccc aaaaccgga cagtcttta gctactttg catgcgcac aaatcgacc gtcatggac ttgaagtgg aacgagaat ggcaattgg ctgataacg gcctcacgt gaccacctt ttaaaactg ttcgatttg aggaacctc aaccaggag gtgcagacc ttccgtgga cacaagaag gaggcttcc agcgtcgcc tggcacccc atccatgag gggttgttt tcgagtggt gggtccgat gggtccatc atgttctgg cacgttgga gctgataag gaagtcggg gctatagaa caagcccat gatagtata gtctggacg ctagcgtgg cacccattg ggtcatatc ctatgttcg ggatcaaat gaccacacc agtaaattc tggaccagg aaccgaccc ggagaccaa atgagggat aaatacaac ttgaacact cttccggcc ggtattgca ggtcttgaa gatttggat atgggtgaa gaaccgtca attattccg ggaatgggt cctgacgat aaggtcgag ttaacttcg ctttcgtcc gatggtaac actgccatt cctggacta gatctcgac ttgttaact acgacgaat gaagagaaa accaaaatt aaaaaagtg ccgtttagt aaaccaatt cctaggaat ttccaagcg caatggaac gaaacttat gaagacgca gccgggacg ataaacgaa gtgattagt caaattgtc gaaaatacg ccaggtgtt gttcccctg cagaaaatt gccccaagt gcgattatt atttacgga aaacttatc ccagttgaa cccggatcg aaactggag caagtgata gccgaaggc cccgaagcc cttcaaaag tacatcgat tcgggtgag atcgaggag ctgcacgac gtgatgccg atcggcgac gacgaggac tacctcaac gactacgat agcaacacc gactcgcct ggaggtact acaacaaac attcccagc ctcatgcaa acaccagca gctccacca atcacccaa gacacaaac ctaacccgc gctcttgat tcagacatg cgtagtgtg gaccaagac atgagatcg ttctcttca tatggccgt gataacgac atgcgcgat cccgattac agaagtgtg gtcaaccgg gagctgaat ttccagcca ccgccacgt gactatgac ataggggat gaggattac cgacaacca tttcggaac gacccggat ttcagacac tatgacgac gaggagggc tatgatgag tattatgat gaggcgagg aactggcca cgcagtaac gggaattac ggccagttt gggggaagg aaccagaac ttcgggggg aatcacccg aacttccgc gggcagttt gagggctgg agcgggcat agtgatttc agggggcgg ggggcgaag cgcggcgcg agggggcag cgcaatccg aggagcagg ccaaggggt ggttcgaga ggaaactca aggggtggg gctccgaac aggggacag tcgtcgtcc agagggcgc ttttaa
- >TC013939 RA
ctgcgcatg cgtgttgca gtgacgaaa attttgcag tttcagaac cagaaatcg gtggttttt tatgttttc tactagaaa ttgttacgt attttcttg ttattttct caaccccga cacttccga gtccatatc actctacaa atacaggcc aagatgctc tgcttcgtg acgttttca agtttattc cacttgaaa tgaatgagc tgtgatcaa caatgatgg agtttgcac aaccgcccc ccaacatgg ccatgcctc ctccgggca tggggcagc agggcatga acatgccac cacctagca tgggcggac ctggagggt accaacaca acaagcctc cgtttcgcc ccttcatga acatgcaaa ggccgctac ctgggcccc tgggcaaca tgaccctcg aagacttcg acgggaagc ggctgagga agtcggtaa tgaggaaaa ctgtagact acaattctt caattataa aggaattac aatcaagag tgtggcaga gggattaca gggacagga gagccttgc aacctgatg tgatgtatt acccagaca tgatgcctc ctccgagtt accaagata accccatta actgtgtca ccactaggt ttgttaaaa ctgcaacaa ataagatga gatgtccca ttttttgta tggcatgga cgccagaag gacggagac ttgtcacag gcgccagct ctggggagt ttacactct ggaatggcc tcaccttca atttcgaaa ctatcttac aagctcatg acagccccg taagaacga tggtctgga gccacaacg atagttgga tggtgacag gcgaccatg cgggttatg tgaaatatt ggcagtcaa atatgaaca atgtgaaga tgttccagg cccataaag aagctctta gaggcataa gcttcagcc cctcggata ccaagtttg taacgtgct cagacgatg gtactctgc gaatttggg actttttcc gataccaag aggagcgga ttctgcgag gtcacgggg ctgatgtaa agtgcgtgc actggcacc cccaaaagg ggcttatcg tttccggta gcaaagaca atcagcagc cgattaagc tctgggacc ccaaaaccg gacagtctt tagctactt tgcatgcgc acaaatcga ccgtcatgg acttgaagt ggaacgaga atggcaatt ggctgataa cggcctcac gtgaccacc ttttaaaac tgttcgatt tgaggaacc tcaaccagg aggtgcaga ccttccgtg gacacaaga aggaggctt ccagcgtcg cctggcacc ccatccatg aggggttgt tttcgagtg gtgggtccg atgggtcca tcatgttct ggcacgttg gagctgata aggaagtcg gggctatag aacaagccc atgatagta tagtctgga cgctagcgt ggcacccat tgggtcata tcctatgtt cgggatcaa atgaccaca ccagtaaat tctggacca ggaaccgac ccggagacc aaatgaggg ataaataca acttgaaca ctcttccgg ccggtattg caggtcttg aagatttgg atatgggtg aagaaccgt caattattc cgggaatgg gtcctgacg ataaggtcg agttaactt cgctttcgt ccgatggta acactgcca ttcctggac tagatctcg acttgttaa ctacgacga atgaagaga aaaccaaaa ttaaaaaag tgccgttta gtaaaccaa ttcctagga atttccaag cgcaatgga acgaaactt atgaagacg cagccggga cgataaacg aagtgatta gtcaaattg tcgaaaata cgccaggtg ttgttcccc tgcagaaaa ttgccccaa gtgcgatta ttatttacg gaaaactta tcccagttg aacccggat cgaaactgg agcaagtga tagccgaag gccccgaag cccttcaaa agtacatcg attcgggtg agatcgagg agctgcacg acgtgatgc cgatcggcg acgacgagg actacctca acgactacg atagcaaca ccgactcgc ctggaggta ctacaacaa acattccca gcctcatgc aaacaccag cagctccac caatcaccc aagacacaa acctaaccc gcgctcttg attcagaca tgcgtagtg tggaccaag acatgagat cgttctctt catatggcc gtgataacg acatgcgcg atcccgatt acagaagtg tggtcaacc gggagctga atttccagc caccgccac gtgactatg acatagggg atgaggatt accgacaac catttcgga acgacccgg atttcagac actatgacg acgaggagg gctatgatg agtattatg atgaggcga ggaactggc cacgcagta acgggaatt acggccagt ttgggggaa ggaaccaga acttcgggg ggaatcacc cgaacttcc gcgggcagt ttgagggct ggagcgggc atagtgatt tcagggggc ggggggcga agcgcggcg cgagggggc agcgcaatc cgaggagca ggccaaggg gtggttcga gaggaaact caaggggtg gggctccga acaggggac agtcgtcgt ccagagggc gcttttaaa cgtagtcat attgattac gagtgcgtg ttagttatt acataccgt ttatttttt ctggaaaaa ctaagacgc tattgcctc tgtggttta ttttcagag atttcttgg ttattttac agtaaaata taacagtat caaagttac accatacat tgtcaagaa caagatatt gtcttcttt ttcagaaat aaacattac gttgattg
