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Gene information
General information
TC013918 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2190919..2196438
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013918
    iBeetle screen iB_05525

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013918 RA
      MGSFKPLLF FAVATLFAA YCKAESKVV CYYDSKSHF 
      RQGQAKFEI TDLEPALQY CTHLIYGYA AIDEETYKL 
      TPLNEQFDV IKDNYRKVT DLKKRFPKL KVLLSVGGN 
      ADVSGQDEE KNIKYRNLL ETTTRRLAF VNSAYTLVK 
      AYGFDGLDL AWEFPENKP KKIRSKLGS IWHSVKKTV 
      AGDKVLDEN AAEHREQFV SLVRELRGA FKAENLLVT 
      LTVLPNVNS TVYYDPRAL SPNLEFVVL EAFDFYTPA 
      RNPKEADYP SPLYELVDR RNDENIDAQ VKYWMSNGA 
      PSAKIVLGL PTFGRAWAM DDESDINGT PPLHISGPA 
      EAGPLTKDA GLLSYPEIC TLLNDPQNA KTQKAFQVK 
      RVPDPSKRK GSYIFRLPD ENNNGGFWV GYEDTDTAG 
      YKASYVKAK GLGGIAIVD LSLDDFKGT CGRDKYDIL 
      RTAKTHL
    CDS sequence
    • >TC013918 RA
      atgggctcc ttcaagcca cttctattt tttgctgta 
      gctacttta ttcgcagct tactgtaaa gccgaaagc 
      aaagttgtt tgttactac gatagcaag tcgcacttc 
      aggcagggg caagcaaaa ttcgaaatc acagaccta 
      gaaccggcc ctgcaatac tgcacccac cttatctac 
      ggctacgcc gccatagac gaggaaact tacaaacta 
      actccactc aatgaacaa ttcgacgta attaaagac 
      aactatcga aaagtcaca gacctcaaa aaacgcttc 
      cctaaactt aaagtccta ctttcggtt ggaggcaac 
      gccgatgtc agcggccaa gacgaagaa aaaaatatc 
      aaataccga aacttattg gagaccaca actcgacga 
      ctcgcgttc gtaaactct gcctacacc cttgtcaaa 
      gcttacggt ttcgatggc ctggacttg gcctgggaa 
      ttccccgag aacaagccg aagaaaatc cgatccaaa 
      cttggaagt atctggcat tcggtgaaa aaaacggta 
      gctggagac aaggtattg gacgagaat gcggccgaa 
      catcgggaa cagtttgtt agtttagtg agggagctt 
      cgaggggct ttcaaagcc gaaaatttg ttggtcact 
      ttaaccgtt ttgcccaat gttaacagc acagtctat 
      tatgaccct cgagcttta tcaccaaac ttggaattt 
      gtcgtgctt gaagcgttc gatttttac acacctgca 
      agaaacccg aaagaagct gattatcct tcgcccctt 
      tacgaattg gttgatcgg agaaatgac gaaaatatc 
      gacgctcaa gtcaagtat tggatgagt aacggggcc 
      cccagcgcc aaaatcgtg ttgggtctc ccaaccttt 
      ggtcgcgct tgggcaatg gacgacgaa tccgacatc 
      aacggcact ccgcctctg cacatctcg ggccctgct 
      gaagcaggc cccctcaca aaagacgcc ggtctttta 
      agctacccc gaaatttgc actttactc aacgaccca 
      caaaacgcc aaaacccag aaagctttc caagtgaaa 
      agagtccca gatccctca aaacgcaaa ggatcctac 
      atttttaga ctccctgat gaaaataac aacggcggt 
      ttttgggtc ggttatgaa gacaccgac acagccgga 
      tataaagcc agttatgtg aaagccaaa ggactggga 
      gggattgca attgttgac ttgtcgttg gacgacttt 
      aagggaact tgcgggcgg gataaatac gatatttta 
      agaaccgct aaaacgcat ttgtaa
    mRNA sequence
    • >TC013918 RA
      attcagtac agcaccaac aacacacca caaaccatg 
      ggctccttc aagccactt ctatttttt gctgtagct 
      actttattc gcagcttac tgtaaagcc gaaagcaaa 
      gttgtttgt tactacgat agcaagtcg cacttcagg 
      caggggcaa gcaaaattc gaaatcaca gacctagaa 
      ccggccctg caatactgc acccacctt atctacggc 
      tacgccgcc atagacgag gaaacttac aaactaact 
      ccactcaat gaacaattc gacgtaatt aaagacaac 
      tatcgaaaa gtcacagac ctcaaaaaa cgcttccct 
      aaacttaaa gtcctactt tcggttgga ggcaacgcc 
      gatgtcagc ggccaagac gaagaaaaa aatatcaaa 
      taccgaaac ttattggag accacaact cgacgactc 
      gcgttcgta aactctgcc tacaccctt gtcaaagct 
      tacggtttc gatggcctg gacttggcc tgggaattc 
      cccgagaac aagccgaag aaaatccga tccaaactt 
      ggaagtatc tggcattcg gtgaaaaaa acggtagct 
      ggagacaag gtattggac gagaatgcg gccgaacat 
      cgggaacag tttgttagt ttagtgagg gagcttcga 
      ggggctttc aaagccgaa aatttgttg gtcacttta 
      accgttttg cccaatgtt aacagcaca gtctattat 
      gaccctcga gctttatca ccaaacttg gaatttgtc 
      gtgcttgaa gcgttcgat ttttacaca cctgcaaga 
      aacccgaaa gaagctgat tatccttcg cccctttac 
      gaattggtt gatcggaga aatgacgaa aatatcgac 
      gctcaagtc aagtattgg atgagtaac ggggccccc 
      agcgccaaa atcgtgttg ggtctccca acctttggt 
      cgcgcttgg gcaatggac gacgaatcc gacatcaac 
      ggcactccg cctctgcac atctcgggc cctgctgaa 
      gcaggcccc ctcacaaaa gacgccggt cttttaagc 
      taccccgaa atttgcact ttactcaac gacccacaa 
      aacgccaaa acccagaaa gctttccaa gtgaaaaga 
      gtcccagat ccctcaaaa cgcaaagga tcctacatt 
      tttagactc cctgatgaa aataacaac ggcggtttt 
      tgggtcggt tatgaagac accgacaca gccggatat 
      aaagccagt tatgtgaaa gccaaagga ctgggaggg 
      attgcaatt gttgacttg tcgttggac gactttaag 
      ggaacttgc gggcgggat aaatacgat attttaaga 
      accgctaaa acgcatttg taaatgtta taaccgatt 
      gtaattatt tttgaaaaa atatacaac attaaataa 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY