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Gene information
General information
TC013915 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2212431..2241225
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013915
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013915 RA
      MPHQWMEGN LPVSAKCAV CDKTCGSVL RLQDWRCLW 
      CRATVHTQC RPTLPPSCP LGPARVSVV PPTALHAIH 
      DEAWEVVRP QASSPLLVF VNSKSGDNQ GVKFLRRFK 
      QLLNPAQVF DLISTGPRL GLRLFRHFD PFRILVCSG 
      DGSVGWVLS EIDKLDMHK QCQVAVLPL GTGNDLARV 
      LGWGSSCDD DTHLPQLLE KYEKAGTKM LDRWSIMAF 
      ERTIAMPKL SLTPGQPEG QLHTQITQY EDSLVTHLQ 
      NILQSDETS VILNSAKRL CETVKEFAT QLSDSSLTR 
      GDEQLGKKC DILQQKLDL LLATLTSEQ LDAAHLDDA 
      KTKQEDTDS EVSFDKAKT EKSEKDLSN FNFRKHRRT 
      SRFMEREKD ALMLRANSL KRAIRNLVE HTEQVVDEQ 
      NRQASSSLP TVKISLSTD LDKEESAKM DSLKVLPTI 
      ESGSSTDVS SCPSPTSSI LTARLANIS PMPDVRRDS 
      TVEDPDLLT TLPVPPDFA DSRRSSQVQ PSSMVDIAT 
      EKILRETCS TLISIGNLK TNEPEVSHD KQRRESTKY 
      DEEEKECSS ILSAISNEE VSITSEILD QKSFCPRKN 
      SDGEDLNGH ICHIDSPET DTYPNSDAL HGESLMDDI 
      SSMLGEVMY GYDQDSIEN TYTDDTTLF TNDMADLKL 
      ERRMSAKRD SLDKKRRSS RSKLDEDTQ FGFENRAFM 
      SQHVYLDNK VESEPTRYC SLAQFVEGN DIARRSFKR 
      RSTKMSKTE ANVTRQSTL LEESEITPV GSFNNLNKL 
      EKELSNVST STLKALELD KETEEEAPK KNKEDALEK 
      LKAEMCVFP QVSVIVEPP SPVLTEEIR TERMVKLGV 
      EIEIDSGSD LDLRKSDKL STDVTTSNN SSSSNLLGI 
      DNEQFMTRS PAATRRISC CSMLNPAEA AALAAAAAT 
      TSFYTDAEK KEKKDEREK ENRKLPIIN PLVRLPTWP 
      NVSTGSGFI SKCLLANAD TLCAAVSPL IDPDETLLE 
      GFYERCVMN NYFGIGIDA KISLDFHHK REEHPEKCR 
      SRAKNYMWY GVLGSKEWL QKTYKNLEQ RVQLECDGQ 
      RIPLPSLQG IVILNIPSF MGGINFWGG HKEDHIFLP 
      PSFDDKILE VVAVFGSVQ MAASRLINL QHHRIAQCQ 
      SIQINILGE EGVPIQVDG EAWIQPPGI IRILHKNRM 
      QMLCRNRAL ENSLKSWEE KQRQSIAAQ GKQRHSISH 
      DKGRHSFTR QSMPSHEKS FLGVNFEKI RQHSFSGAV 
      PVHHHEKTV TFVEKPITP IEPDILFTD EEHHLLLSF 
      IECVTTLTK WIKILAISH SLEADLYSL AAKCDTCLE 
      NIHPNGKIL EGPTLRTEF TKLVTAVKQ LYEESCCLL 
      HDRGDKLKL REDLENKLS ASLANTEME LRKCVMYDK 
      PQGSLVYLQ PVQGDQSEH KRKGLFWLK FRTKGASSS 
      QQQGGKKTS REVASWGTQ EVATWLETL QLSEYIDSF 
      VKNDIRGRE LLTLARRDL KDLGVTKVG HVKRILQAI 
      KDLTQGL
    CDS sequence
    • >TC013915 RA
      atgccgcat cagtggatg gagggaaat ttgccagtt 
      tctgccaaa tgcgctgtt tgtgataaa acttgcggt 
      tcagtactt agattacaa gattggcgg tgtctgtgg 
      tgtcgcgcc acagttcac acccagtgc agacccacc 
      ctgcctccg tcgtgtccg ttgggtccg gcgagggtg 
      agtgtggtg ccgcccacg gccctgcac gccatccac 
      gacgaggcc tgggaagtc gtcagaccg caagcttct 
      tcacccctg ctagtcttc gtaaattcc aagtcaggt 
      gataatcaa ggcgtaaag tttctgcga aggttcaaa 
      caattactc aatcccgca caagttttc gatctcatt 
      tcgaccggg cccagatta ggtttaaga ctttttcga 
      cacttcgat ccctttcgc attttggtc tgcagtggg 
      gacgggtct gtgggatgg gtgctgtcc gagattgac 
      aagctcgac atgcacaaa cagtgccag gtggcggtg 
      cttcccttg ggcacaggc aacgacctg gcccgtgtc 
      ctgggctgg ggttcatcc tgcgacgac gacacccac 
      ttgccacag ctgttggaa aaatacgag aaagccgga 
      actaagatg ctcgatcgt tggtcaata atggccttc 
      gagcgcacc atagccatg cctaagctg tccctaacc 
      ccaggtcaa cccgaaggt caattacac acccaaatc 
      acccaatac gaagactcc ctggttacc cacctgcaa 
      aacatcctc caatccgac gaaacttca gtgattctg 
      aactcagca aagcgatta tgcgaaacg gtgaaggag 
      tttgctacc caactttcg gacagttct ctgacacga 
      ggtgacgag cagttgggc aaaaagtgt gatatttta 
      caacagaag ttagattta ttattagct acgttaacc 
      agcgaacaa ttagatgcg gcacactta gacgatgcc 
      aaaacgaaa caagaagat accgacagc gaagtgtct 
      ttcgataaa gccaaaacg gaaaaaagc gaaaaagat 
      ttgagcaat tttaacttt aggaaacac agacgcact 
      tccagattt atggagcgt gagaaggat gctctcatg 
      ttaagggcc aacagtctc aaaagggca atacgtaat 
      ctggtggag cacacggag caagtggtg gatgagcaa 
      aacagacaa gcttcatcc agtttacca acggtgaaa 
      atctctcta agtactgat ttggataag gaagaatcg 
      gccaaaatg gactctctt aaagtgcta ccgacgatt 
      gaatctggg tctagcacc gacgtcagc tcttgccca 
      tcacccact agctccatt cttacagcc aggttagcc 
      aacatatca ccaatgccc gatgttagg agagactca 
      acagtcgaa gatcctgat cttttgacc accctgcca 
      gttccaccg gattttgca gacagtcgg aggtccagt 
      caagtccaa ccaagcagt atggtggac attgcaacg 
      gagaaaatc ttgcgtgaa acttgctca acactgata 
      tccataggc aatctaaaa accaacgaa ccggaggta 
      agtcatgac aaacaacga cgcgaaagt acaaagtac 
      gacgaagaa gagaaagag tgcagtagc atcctatct 
      gcgatttct aacgaagaa gttagcata acatcggaa 
      atcctggac cagaaatcg ttttgtccc cgaaaaaac 
      agcgacggg gaagattta aacggccat atctgccac 
      atagactcg ccagaaacg gacacttat cccaattcg 
      gacgccctg catggagag tccctcatg gacgacata 
      agctcgatg ttgggagaa gtgatgtac ggctacgac 
      caagacagc atcgagaac acctacact gacgatacc 
      acgcttttc acaaacgac atggctgat ttgaaactt 
      gagagaaga atgagtgcg aagagggac tccctggac 
      aagaaacga agaagctcc agaagcaaa ctggacgaa 
      gacacgcaa tttggattt gaaaatcgt gcctttatg 
      tcacaacat gtataccta gataataaa gtagaatct 
      gaaccgacg aggtactgc agtttggcc caattcgtt 
      gaaggtaac gacatagca aggaggtcg ttcaagagg 
      cgatcgacc aaaatgtca aagaccgaa gcaaatgtc 
      accagacaa agcactctg cttgaggaa agtgaaata 
      acgccagtt gggtcattc aataacttg aacaaactg 
      gagaaggag ttgtcaaac gtgtccacc tcgaccctc 
      aaagccctg gagctggac aaggaaacg gaagaggaa 
      gcgccaaaa aagaacaaa gaggatgct ctcgagaag 
      ttgaaagct gaaatgtgt gtcttcccg caagtgagc 
      gtaatagtg gagccccct tcacccgtc ttgactgaa 
      gagattcga accgaacgc atggtgaaa ctaggtgtt 
      gaaattgag atagattct ggttctgac ttggatttg 
      cgtaaatcc gacaagcta tcgactgat gtcaccacc 
      agcaataac agttccagt tcgaatttg cttggaatt 
      gacaacgag caatttatg acgcgatca cctgcagca 
      acacgcagg atttcctgc tgcagtatg ttaaatcct 
      gctgaagct gcggctctg gcagctgca gctgcaacg 
      accagtttc tacaccgac gcggaaaag aaggagaaa 
      aaggatgag cgggagaag gaaaacagg aagctgcca 
      atcatcaat ccactagtg cgactacca acatggcca 
      aatgtgagc acgggaagt ggtttcatt agcaaatgt 
      cttctagct aatgctgac accctttgc gcagcagta 
      agtccccta attgaccct gatgaaaca ctactagaa 
      ggtttctac gaacgctgc gtcatgaac aactatttc 
      ggtatcgga atcgacgcc aaaatctcg cttgatttc 
      caccacaaa cgcgaggaa catcccgaa aagtgcagg 
      tcgagagct aaaaactac atgtggtac ggggttttg 
      gggagcaaa gagtggctc cagaaaacg tacaaaaat 
      ctagaacaa agggtccaa ctggagtgt gacgggcag 
      agaataccc ttgcccagt cttcaggga atcgtcata 
      ctcaatata ccaagtttc atgggcggc atcaatttt 
      tggggcggc cacaaagaa gaccacatt ttcctccca 
      ccgtcattc gacgacaaa atcctggaa gtggttgca 
      gttttcggt tcggtccaa atggcagct tcgcgtctc 
      atcaacttg caacatcac cgaatcgcc caatgccag 
      agcatccag atcaacatc ttgggcgaa gaaggcgtc 
      cccattcaa gtagacggc gaagcctgg atccagcct 
      ccaggcatc atccgaatc ctccacaaa aaccgaatg 
      cagatgctt tgcagaaac cgggccttg gagaactcc 
      ctcaaatca tgggaggaa aagcagaga cagagcata 
      gccgcccag ggcaagcaa agacattcc atttcgcac 
      gataaaggc cgccacagc ttcactcgc caaagtatg 
      ccaagccat gaaaagtcg tttttggga gtcaatttc 
      gaaaaaatt cgccagcat tcgttcagc ggtgccgtt 
      ccggtacat caccacgaa aaaactgtc acttttgtc 
      gaaaaaccc atcactcca atcgaaccg gatatcttg 
      ttcaccgac gaagagcat cacttgctg cttagtttc 
      atcgaatgt gtgactact ttgaccaaa tggattaaa 
      atcctagct attagtcat tcgctagaa gccgatttg 
      tattcacta gcagcgaaa tgtgacact tgtcttgaa 
      aatatacac ccgaatggg aaaatactc gaagggcct 
      acgctacga actgaattt actaaactg gtcactgct 
      gttaaacaa ctgtatgaa gagagttgc tgtcttttg 
      cacgataga ggcgataag ttgaaactg cgcgaagat 
      ttagaaaat aagctaagt gccagttta gccaacaca 
      gagatggaa ttaaggaag tgtgtgatg tacgacaaa 
      ccgcaggga agtctggtt tatctacaa ccagtgcaa 
      ggcgaccag tctgagcat aaacgcaag ggcctcttc 
      tggctgaag ttccgtaca aagggcgca agttcgagc 
      cagcagcaa ggcgggaag aagacgagc cgtgaagtg 
      gcatcgtgg ggtacgcaa gaggtggca acttggtta 
      gaaactcta caactgtcc gaatacatc gacagtttt 
      gttaagaac gacatacga ggccgcgag cttctgaca 
      ttggcaagg agggatttg aaagactta ggcgtgact 
      aaagtagga catgttaaa agaatactg caagccatt 
      aaggatttg acccaaggt ttataa
    mRNA sequence
    • >TC013915 RA
      tccccccaa tgaacagaa tataacaat gccgcatca 
      gtggatgga gggaaattt gccagtttc tgccaaatg 
      cgctgtttg tgataaaac ttgcggttc agtacttag 
      attacaaga ttggcggtg tctgtggtg tcgcgccac 
      agttcacac ccagtgcag acccaccct gcctccgtc 
      gtgtccgtt gggtccggc gagggtgag tgtggtgcc 
      gcccacggc cctgcacgc catccacga cgaggcctg 
      ggaagtcgt cagaccgca agcttcttc acccctgct 
      agtcttcgt aaattccaa gtcaggtga taatcaagg 
      cgtaaagtt tctgcgaag gttcaaaca attactcaa 
      tcccgcaca agttttcga tctcatttc gaccgggcc 
      cagattagg tttaagact ttttcgaca cttcgatcc 
      ctttcgcat tttggtctg cagtgggga cgggtctgt 
      gggatgggt gctgtccga gattgacaa gctcgacat 
      gcacaaaca gtgccaggt ggcggtgct tcccttggg 
      cacaggcaa cgacctggc ccgtgtcct gggctgggg 
      ttcatcctg cgacgacga cacccactt gccacagct 
      gttggaaaa atacgagaa agccggaac taagatgct 
      cgatcgttg gtcaataat ggccttcga gcgcaccat 
      agccatgcc taagctgtc cctaacccc aggtcaacc 
      cgaaggtca attacacac ccaaatcac ccaatacga 
      agactccct ggttaccca cctgcaaaa catcctcca 
      atccgacga aacttcagt gattctgaa ctcagcaaa 
      gcgattatg cgaaacggt gaaggagtt tgctaccca 
      actttcgga cagttctct gacacgagg tgacgagca 
      gttgggcaa aaagtgtga tattttaca acagaagtt 
      agatttatt attagctac gttaaccag cgaacaatt 
      agatgcggc acacttaga cgatgccaa aacgaaaca 
      agaagatac cgacagcga agtgtcttt cgataaagc 
      caaaacgga aaaaagcga aaaagattt gagcaattt 
      taactttag gaaacacag acgcacttc cagatttat 
      ggagcgtga gaaggatgc tctcatgtt aagggccaa 
      cagtctcaa aagggcaat acgtaatct ggtggagca 
      cacggagca agtggtgga tgagcaaaa cagacaagc 
      ttcatccag tttaccaac ggtgaaaat ctctctaag 
      tactgattt ggataagga agaatcggc caaaatgga 
      ctctcttaa agtgctacc gacgattga atctgggtc 
      tagcaccga cgtcagctc ttgcccatc acccactag 
      ctccattct tacagccag gttagccaa catatcacc 
      aatgcccga tgttaggag agactcaac agtcgaaga 
      tcctgatct tttgaccac cctgccagt tccaccgga 
      ttttgcaga cagtcggag gtccagtca agtccaacc 
      aagcagtat ggtggacat tgcaacgga gaaaatctt 
      gcgtgaaac ttgctcaac actgatatc cataggcaa 
      tctaaaaac caacgaacc ggaggtaag tcatgacaa 
      acaacgacg cgaaagtac aaagtacga cgaagaaga 
      gaaagagtg cagtagcat cctatctgc gatttctaa 
      cgaagaagt tagcataac atcggaaat cctggacca 
      gaaatcgtt ttgtccccg aaaaaacag cgacgggga 
      agatttaaa cggccatat ctgccacat agactcgcc 
      agaaacgga cacttatcc caattcgga cgccctgca 
      tggagagtc cctcatgga cgacataag ctcgatgtt 
      gggagaagt gatgtacgg ctacgacca agacagcat 
      cgagaacac ctacactga cgataccac gcttttcac 
      aaacgacat ggctgattt gaaacttga gagaagaat 
      gagtgcgaa gagggactc cctggacaa gaaacgaag 
      aagctccag aagcaaact ggacgaaga cacgcaatt 
      tggatttga aaatcgtgc ctttatgtc acaacatgt 
      atacctaga taataaagt agaatctga accgacgag 
      gtactgcag tttggccca attcgttga aggtaacga 
      catagcaag gaggtcgtt caagaggcg atcgaccaa 
      aatgtcaaa gaccgaagc aaatgtcac cagacaaag 
      cactctgct tgaggaaag tgaaataac gccagttgg 
      gtcattcaa taacttgaa caaactgga gaaggagtt 
      gtcaaacgt gtccacctc gaccctcaa agccctgga 
      gctggacaa ggaaacgga agaggaagc gccaaaaaa 
      gaacaaaga ggatgctct cgagaagtt gaaagctga 
      aatgtgtgt cttcccgca agtgagcgt aatagtgga 
      gcccccttc acccgtctt gactgaaga gattcgaac 
      cgaacgcat ggtgaaact aggtgttga aattgagat 
      agattctgg ttctgactt ggatttgcg taaatccga 
      caagctatc gactgatgt caccaccag caataacag 
      ttccagttc gaatttgct tggaattga caacgagca 
      atttatgac gcgatcacc tgcagcaac acgcaggat 
      ttcctgctg cagtatgtt aaatcctgc tgaagctgc 
      ggctctggc agctgcagc tgcaacgac cagtttcta 
      caccgacgc ggaaaagaa ggagaaaaa ggatgagcg 
      ggagaagga aaacaggaa gctgccaat catcaatcc 
      actagtgcg actaccaac atggccaaa tgtgagcac 
      gggaagtgg tttcattag caaatgtct tctagctaa 
      tgctgacac cctttgcgc agcagtaag tcccctaat 
      tgaccctga tgaaacact actagaagg tttctacga 
      acgctgcgt catgaacaa ctatttcgg tatcggaat 
      cgacgccaa aatctcgct tgatttcca ccacaaacg 
      cgaggaaca tcccgaaaa gtgcaggtc gagagctaa 
      aaactacat gtggtacgg ggttttggg gagcaaaga 
      gtggctcca gaaaacgta caaaaatct agaacaaag 
      ggtccaact ggagtgtga cgggcagag aataccctt 
      gcccagtct tcagggaat cgtcatact caatatacc 
      aagtttcat gggcggcat caatttttg gggcggcca 
      caaagaaga ccacatttt cctcccacc gtcattcga 
      cgacaaaat cctggaagt ggttgcagt tttcggttc 
      ggtccaaat ggcagcttc gcgtctcat caacttgca 
      acatcaccg aatcgccca atgccagag catccagat 
      caacatctt gggcgaaga aggcgtccc cattcaagt 
      agacggcga agcctggat ccagcctcc aggcatcat 
      ccgaatcct ccacaaaaa ccgaatgca gatgctttg 
      cagaaaccg ggccttgga gaactccct caaatcatg 
      ggaggaaaa gcagagaca gagcatagc cgcccaggg 
      caagcaaag acattccat ttcgcacga taaaggccg 
      ccacagctt cactcgcca aagtatgcc aagccatga 
      aaagtcgtt tttgggagt caatttcga aaaaattcg 
      ccagcattc gttcagcgg tgccgttcc ggtacatca 
      ccacgaaaa aactgtcac ttttgtcga aaaacccat 
      cactccaat cgaaccgga tatcttgtt caccgacga 
      agagcatca cttgctgct tagtttcat cgaatgtgt 
      gactacttt gaccaaatg gattaaaat cctagctat 
      tagtcattc gctagaagc cgatttgta ttcactagc 
      agcgaaatg tgacacttg tcttgaaaa tatacaccc 
      gaatgggaa aatactcga agggcctac gctacgaac 
      tgaatttac taaactggt cactgctgt taaacaact 
      gtatgaaga gagttgctg tcttttgca cgatagagg 
      cgataagtt gaaactgcg cgaagattt agaaaataa 
      gctaagtgc cagtttagc caacacaga gatggaatt 
      aaggaagtg tgtgatgta cgacaaacc gcagggaag 
      tctggttta tctacaacc agtgcaagg cgaccagtc 
      tgagcataa acgcaaggg cctcttctg gctgaagtt 
      ccgtacaaa gggcgcaag ttcgagcca gcagcaagg 
      cgggaagaa gacgagccg tgaagtggc atcgtgggg 
      tacgcaaga ggtggcaac ttggttaga aactctaca 
      actgtccga atacatcga cagttttgt taagaacga 
      catacgagg ccgcgagct tctgacatt ggcaaggag 
      ggatttgaa agacttagg cgtgactaa agtaggaca 
      tgttaaaag aatactgca agccattaa ggatttgac 
      ccaaggttt ataaattcc aaatgcact gaccaactt 
      atttgatca aaaacgttg ggttgagaa acggttcgg 
      ggccagttt tatgaaact agtgttggt cattgcatt 
      ccaatgtgt taattttcg gcgtttaaa atggtacca 
      ccccgtata aatgtactc atcgttgac cgatattag 
      ttattgata tgagaaatt gctgtgatt tccagaata 
      tatgtatat aaaagttat tctagttag gtgtaatgt 
      acgttcgtt catcagatc gaaattcat ctattcttc 
      aatataaaa ccaaaaata atgaaaaat tttactaaa 
      aatcgtacg cttataaaa ctcgttaac aaaatttta 
      actgttaag tctctcttt gaaaagcaa aattttatt 
      attacgaca aactaatat tctaaacta aaaagcaat 
      tatctttcc ctagcaata tatctcaac tgttctatc 
      gctaagtgc attgctttt acttcatct tcgaatcag 
      acctcgagt cgttaaggc tgaccatac tgggaatac 
      ctaacagta tggttcaac cttaaccac ttttcttcg 
      aatcgctgc gttttcacc tgcaaattc ctcgaatat 
      gccattttg attggtccg ctcacgcgc cgcaacaac 
      caatcagga tgccggttt cgaccaact cttcacttc 
      atacttcat ctaggtgac cttacgtgt gtataggag 
      aaaagtcta gttttcatt atttgtact acctatatt 
      tttaagctt ttttggatg ttgaagagt atttataaa 
      acatagaac tagtttagt accttagga taagaatct 
      acgctatga tatactgtg ttagtcatt ttgcgttga 
      ccatttcag agaacgagt tatatagaa gtaactaag 
      ataatcatt acttattgc cttttgcat gttagccta 
      atgtgatta ttgtgtcaa accttagct tcctaagaa 
      ctattttaa gcgctcctg acggagctg tagattatg 
      ttcgcacaa ttaccatat caaagacag gtcattcca 
      tcatgtcct gaaacgacc tgtccttat cggaaaact 
      atttcttag agtagctgt aacaaaacc acagattta 
      ttggtactc ctatcaaga aggtaaact gactgccat 
      gaacagcgc acttcaacc aaacttcct gtgaacgtg 
      ttatgtatt acttagcac aaatatgtt gtagcctta 
      cgatcattc ccgtatgta caaatgtct agatttagg 
      aaatttcgg caaactgat attttgcat tcctttctt 
      cacgttgtt tgtttctcc tgatttctc cacttcgta 
      caatcattg atcaaacgg aaagaaaca caaacaagt 
      ggaccaaat tttcccaaa cactgatcg gtggcggaa 
      gtggcccag ttttaaaca ccacaaccg ccaccgctc 
      gagaccgcc tatcacttt cttcagccc tgtgattgt 
      cttctgttc accgaattc gtattattt gggcggatt 
      tccagaact ttattaagc cacttaaaa acgctctca 
      aaatccgcc tgtattttt ttacctaag actgctgtt 
      gaacttctg tgatataat gttgcagtc acttgtaat 
      aaaatggcc agcaatacg actcttttc gttcattgt 
      tacatttct cctatttcg cgtctttgg acgcaaaat 
      aggaaaaat agtaaattg ttacacaag actcttcaa 
      gcaatagtt tgttacttc acaccattg cttgaatga 
      atgccttct gcaacttgt gtttggttt ttgaacctc 
      aactcttga gtaaaatat gtatgtaaa ttattttac 
      taggaaaag gacgaactc atgctaaat tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY