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Gene information
General information
TC013912 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 218977..235632
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013912
    iBeetle screen iB_05522

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    mature egg deposition number 2nd lateral oviduct: 3 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1

    mature egg deposition 2 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    egg chamber late partiallydegenerated - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: The abdominal musculatur is increased by one segment. The urogomphy seem to be absent.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    mesothoracic tracheal opening ectopically present - Penetrance (percentage of animals/eggs on slide): more than 80%

    prothorax potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs pretarsus distal duplicated - Penetrance (percentage of animals/eggs on slide): less than 30%

    urogomphi not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    urogomphi potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    pygopodium segment potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 4

    antenna orientation irregular - Number of animals showing the phenotype: 4
    elytra partially blistered - Number of animals showing the phenotype: 3

    wings orientation irregular - Number of animals showing the phenotype: 4

    legs orientation irregular - Number of animals showing the phenotype: 4

    pronotum slightly size decreased - Number of animals showing the phenotype: 4
    pronotum shape irregular - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles median shortened - Number of animals showing the phenotype: 3

    oblique dorsal thoracic muscles median orientation irregular - Number of animals showing the phenotype: 3
    abdominal segment 2 gin-traps anterior decreased - Number of animals showing the phenotype: 4

    female genital lobes shape irregular - Number of animals showing the phenotype: 4

    female genital lobes size increased - Number of animals showing the phenotype: 4
    female genital lobes with protrusion - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013912 RA
      MEIDVTPEA SDGASSQGE EENAEKVVS EKAAPEPTK 
      KAIKLALRQ QAKRRKKNT TIASGNSST VPRIIVKPL 
      PPQPPEETP PAKAPTMRE VLASIPGFT MKPRRRTNK 
      KLSTAAQLE QTKEGCIDL ETPDSILVN TNLRLLLNK 
      ATFASLPLL YQNKLVQLL PDVDRNNSV TNSTDSLEL 
      SNSGLNNEF FARACVEWQ DRLAEGEFT PENQQKLKL 
      EADKERSKL DPWKLKHFE PIWGDRSQT EPPQNFNTP 
      LASRPPIKT TIKLRPTTT VTNKAKAAP PIKRLRTVG 
      AMTRSCTSL KESEISNQE PIKTPIPDL LPIKSIRNQ 
      TPKDTSETQ AEKQENCII NISKTILQT DEETIICSN 
      NNKRRRSLS HESIDSKRQ SLDDKVTEP CNTDPDSVD 
      SSIVLSESD TKDEEEPST DEKVEDEEP KTEPKIDEE 
      EEEENPAPN LIEDSFPIL PNSLILQQE SIVLEKTLC 
      DAGMCLEDS AEKLEASVE ESDLVMAQL TESNFESVR 
      NIADDDANE DRFIDAENY VLESGQITV QEADKEKER 
      TEVDIEATL FGNDTAQAP TEGCCWDLV DSSTEKLLE 
      VPLHALESV PAGIVGQEN PTEHVEVIP MQEELEVRL 
      EQGNFPVPN DWPYDVKMD SEMVAAALG TTDNDNATN 
      ATTTSNNKP NQNYQEFTG NQVKLELEV TLTPEIISN 
      DSLITSTVS SSNGTNSTV TPSVSKTVT TVIPPTTIV 
      CLPSVVSTS SLVNPPVNE TQCPTLPRP GMVQSSSAL 
      PYLALSTSQ PIRAVPAHT KSKPKNSQS SGSNRNRGS 
      NKPPPGAVN LERSYQICQ AVIQNSPNR DQLRCQLKP 
      PPSLLAAAA GNNNVKKNE NNRQTQYSS VTSSRGSTK 
      TFTPPLPAS GNFQMVQNN GVKNVGQKA RSVPYQQQR 
      QPSPPVVVR HVFTSGQGI PVTMAVLPQ PPALSPEVV 
      EAPNPMGAV GQYILVQRA GVGDHHNIP RSSSAPPSH 
      QQQLPTPIN TGGVSQIVS VGTARGRPA SVDADHPIL 
      QQQPPPQPP QSNDFIVQC PNPGTQAVT RRQRLPSGV 
      VYGDVSVEN QMHNYTIIG GEMVDHPGA AMQPMQMMP 
      KRTTDNCAC SLKAMVVCK KCGAFCHDD CIGPNRLCR 
      TCFIR
    CDS sequence
    • >TC013912 RA
      atggaaata gacgttaca cccgaggcg agtgacggt 
      gcttcgagt caaggcgag gaagaaaat gccgaaaaa 
      gtggtgtca gaaaaagcg gcccccgag cccacgaag 
      aaggccatc aagctcgcg ctgcgacag caggccaaa 
      cgcaggaaa aagaacacg acgattgca tcgggaaac 
      agttcgacg gttcctcgt ataatagtc aaaccgttg 
      cctccgcag ccccccgag gagacgccc ccagccaaa 
      gcccctacg atgcgggaa gtcctagcg tcgattccc 
      ggcttcact atgaaaccc agacgacgc actaacaag 
      aaactttca actgcagcg caattggaa caaaccaaa 
      gagggctgc atcgatttg gagactcct gactcgatc 
      cttgtcaac acaaacctg cgattacta ctcaacaaa 
      gcgactttt gcctctcta ccacttctg taccaaaac 
      aaactagtg caattatta ccagacgtt gatcgaaat 
      aatagtgta acaaattcg acggattcg ctcgaactt 
      agcaattcc ggactcaat aacgagttt tttgcacgg 
      gcttgtgtc gaatggcaa gatcggctc gccgaaggc 
      gaattcaca ccagaaaac caacaaaaa ttgaaattg 
      gaagctgac aaagaacgt agtaaatta gatccctgg 
      aaattgaaa catttcgaa ccgatttgg ggcgacagg 
      agtcaaact gagccgcct caaaacttc aacacaccc 
      ctggcatca agaccgcca atcaaaacc acgatcaaa 
      ttacgaccc acaacaaca gtcaccaat aaagcaaaa 
      gcagcgcca cctatcaaa cgactacga accgtcgga 
      gcgatgact agatcgtgc acaagtttg aaagaatcc 
      gaaatatcg aatcaagaa ccgatcaag acaccaatc 
      cccgatttg ttaccgatc aaatcgatc cgaaaccaa 
      acgcctaaa gacaccagc gaaacccaa gccgaaaaa 
      caagaaaac tgtataatc aacattagt aaaacgatt 
      cttcaaacc gacgaagag acaatcatt tgtagtaac 
      aataacaag cgtcggaga tcgcttagt cacgaatcc 
      atcgattca aagcgccaa tcgcttgac gataaagtt 
      acagaaccc tgtaatacc gaccctgat tccgtcgac 
      agctcgatc gtgctgtct gaaagcgac accaaagac 
      gaggaggag ccttcgacc gatgaaaag gtcgaagac 
      gaggagcca aaaactgag cctaagatc gacgaagag 
      gaggaggag gaaaacccc gcaccaaac ttaatcgag 
      gactcgttt ccgattctt cccaattcg ttgattttg 
      cagcaggag tcgatcgtg ttggagaag acgctgtgc 
      gacgccggg atgtgtctg gaggactcg gcggagaag 
      ctcgaggcg agtgtggag gagtccgat ttagtgatg 
      gcgcagttg accgaaagc aatttcgaa agcgtgagg 
      aatatcgct gatgatgat gcgaatgag gataggttt 
      atcgatgcg gagaattat gtgctagag tcgggacag 
      attacggtc caggaggcc gataaggaa aaggagagg 
      actgaggtg gatatagag gcgacgttg tttgggaac 
      gacacagcg caagcgcca acagaaggc tgctgttgg 
      gatttagtc gattcgagc accgagaaa cttctagaa 
      gtgcctttg cacgcgtta gagtctgtt cctgccggc 
      atcgtcggt caggagaat cccaccgaa cacgtcgaa 
      gtgattccg atgcaagaa gaactcgaa gtgagacta 
      gaacaagga aatttccct gttccaaac gattggcct 
      tacgatgtg aaaatggat tcggaaatg gtggcagcg 
      gctttggga accactgac aatgataac gccaccaac 
      gccaccacc acctccaat aacaaaccg aaccaaaac 
      tatcaggaa ttcaccgga aatcaagtc aaactcgag 
      ctggaagtg actctaaca ccggaaatt attagtaac 
      gatagtctc ataacaagt acagtcagt tcgagcaat 
      ggaacgaat tcgactgtc acgccctct gttagtaag 
      acagtaacg actgtgatt ccgccaact accatcgtt 
      tgtttgcct tcggttgtc agtacttcg tctctggtg 
      aatccccca gttaatgaa acgcaatgt cctacttta 
      ccgaggcct ggaatggta caaagttcg agtgcttta 
      ccttatctg gcattgagt acgagtcaa ccaatcagg 
      gctgtaccg gcacacacc aaatcaaaa ccgaagaat 
      tcgcaaagt agtggttcg aatcgaaat agagggagc 
      aacaaaccg ccaccgggg gctgtcaat ttagaaagg 
      agttatcag atttgtcaa gctgtcatt cagaatagt 
      cccaatagg gatcagctc aggtgtcag ttgaagcca 
      cccccatct cttcttgcc gccgccgca ggtaacaac 
      aacgtgaaa aagaacgaa aacaataga caaactcag 
      tacagttca gtgacttcg tctcgaggt tcaactaag 
      acgtttacg cccccattg cctgccagt ggtaacttc 
      caaatggtg caaaataat ggcgttaaa aacgtagga 
      cagaaggcg aggtcagtt ccttatcaa caacagagg 
      caaccaagt ccacctgtg gtggtgcga cacgtcttc 
      acatcagga cagggaatt cctgtcact atggctgtg 
      cttccacaa cctcctgcc ttaagtcca gaagtggtt 
      gaagccccg aatccgatg ggcgccgtc ggccaatac 
      atcctcgtg cagcgcgct ggcgtcggc gaccaccac 
      aacatcccc cgttcgtcc agcgcaccc ccatcccac 
      cagcagcag ctcccgacg ccgatcaac actggtggc 
      gtctcgcag atagtttca gtgggtaca gcgcgcggt 
      cgtccggcc agtgtcgac gcggaccat ccgatcctt 
      caacagcag ccaccgccc cagccgcca caatcgaac 
      gactttatt gttcagtgt ccgaatccg ggcactcaa 
      gcggtcacg cgcagacag agactgcct tccggtgta 
      gtgtacggc gacgtaagc gttgagaat caaatgcat 
      aattatacg atcattggc ggcgagatg gttgaccat 
      ccgggggcg gcgatgcag ccgatgcaa atgatgccg 
      aagcggacg acggacaat tgcgcgtgc agtctgaaa 
      gcgatggtc gtatgtaaa aagtgcgga gcgttctgt 
      cacgacgac tgtattgga ccgaataga ctttgtcgt 
      acgtgtttt atacggtag
    mRNA sequence
    • >TC013912 RA
      ccggttatg aaatggcgg atggtgttt tattgtcaa 
      agaccgagt gcttttaaa caaaaagac taagactgt 
      tgtcgcctc ctagcattt tgtaaatga atggcaacg 
      agtcctgtt gtgacccga acgcgcacc aaaatatgg 
      ggtgaatgc tcgcctgaa aattcggtc gtatttcac 
      acacaagtc gaaaaaatc gactcaagt aaactttaa 
      tgtcctcaa ctgttcggt tacgtgatg gaaatagac 
      gttacaccc gaggcgagt gacggtgct tcgagtcaa 
      ggcgaggaa gaaaatgcc gaaaaagtg gtgtcagaa 
      aaagcggcc cccgagccc acgaagaag gccatcaag 
      ctcgcgctg cgacagcag gccaaacgc aggaaaaag 
      aacacgacg attgcatcg ggaaacagt tcgacggtt 
      cctcgtata atagtcaaa ccgttgcct ccgcagccc 
      cccgaggag acgccccca gccaaagcc cctacgatg 
      cgggaagtc ctagcgtcg attcccggc ttcactatg 
      aaacccaga cgacgcact aacaagaaa ctttcaact 
      gcagcgcaa ttggaacaa accaaagag ggctgcatc 
      gatttggag actcctgac tcgatcctt gtcaacaca 
      aacctgcga ttactactc aacaaagcg acttttgcc 
      tctctacca cttctgtac caaaacaaa ctagtgcaa 
      ttattacca gacgttgat cgaaataat agtgtaaca 
      aattcgacg gattcgctc gaacttagc aattccgga 
      ctcaataac gagtttttt gcacgggct tgtgtcgaa 
      tggcaagat cggctcgcc gaaggcgaa ttcacacca 
      gaaaaccaa caaaaattg aaattggaa gctgacaaa 
      gaacgtagt aaattagat ccctggaaa ttgaaacat 
      ttcgaaccg atttggggc gacaggagt caaactgag 
      ccgcctcaa aacttcaac acacccctg gcatcaaga 
      ccgccaatc aaaaccacg atcaaatta cgacccaca 
      acaacagtc accaataaa gcaaaagca gcgccacct 
      atcaaacga ctacgaacc gtcggagcg atgactaga 
      tcgtgcaca agtttgaaa gaatccgaa atatcgaat 
      caagaaccg atcaagaca ccaatcccc gatttgtta 
      ccgatcaaa tcgatccga aaccaaacg cctaaagac 
      accagcgaa acccaagcc gaaaaacaa gaaaactgt 
      ataatcaac attagtaaa acgattctt caaaccgac 
      gaagagaca atcatttgt agtaacaat aacaagcgt 
      cggagatcg cttagtcac gaatccatc gattcaaag 
      cgccaatcg cttgacgat aaagttaca gaaccctgt 
      aataccgac cctgattcc gtcgacagc tcgatcgtg 
      ctgtctgaa agcgacacc aaagacgag gaggagcct 
      tcgaccgat gaaaaggtc gaagacgag gagccaaaa 
      actgagcct aagatcgac gaagaggag gaggaggaa 
      aaccccgca ccaaactta atcgaggac tcgtttccg 
      attcttccc aattcgttg attttgcag caggagtcg 
      atcgtgttg gagaagacg ctgtgcgac gccgggatg 
      tgtctggag gactcggcg gagaagctc gaggcgagt 
      gtggaggag tccgattta gtgatggcg cagttgacc 
      gaaagcaat ttcgaaagc gtgaggaat atcgctgat 
      gatgatgcg aatgaggat aggtttatc gatgcggag 
      aattatgtg ctagagtcg ggacagatt acggtccag 
      gaggccgat aaggaaaag gagaggact gaggtggat 
      atagaggcg acgttgttt gggaacgac acagcgcaa 
      gcgccaaca gaaggctgc tgttgggat ttagtcgat 
      tcgagcacc gagaaactt ctagaagtg cctttgcac 
      gcgttagag tctgttcct gccggcatc gtcggtcag 
      gagaatccc accgaacac gtcgaagtg attccgatg 
      caagaagaa ctcgaagtg agactagaa caaggaaat 
      ttccctgtt ccaaacgat tggccttac gatgtgaaa 
      atggattcg gaaatggtg gcagcggct ttgggaacc 
      actgacaat gataacgcc accaacgcc accaccacc 
      tccaataac aaaccgaac caaaactat caggaattc 
      accggaaat caagtcaaa ctcgagctg gaagtgact 
      ctaacaccg gaaattatt agtaacgat agtctcata 
      acaagtaca gtcagttcg agcaatgga acgaattcg 
      actgtcacg ccctctgtt agtaagaca gtaacgact 
      gtgattccg ccaactacc atcgtttgt ttgccttcg 
      gttgtcagt acttcgtct ctggtgaat cccccagtt 
      aatgaaacg caatgtcct actttaccg aggcctgga 
      atggtacaa agttcgagt gctttacct tatctggca 
      ttgagtacg agtcaacca atcagggct gtaccggca 
      cacaccaaa tcaaaaccg aagaattcg caaagtagt 
      ggttcgaat cgaaataga gggagcaac aaaccgcca 
      ccgggggct gtcaattta gaaaggagt tatcagatt 
      tgtcaagct gtcattcag aatagtccc aatagggat 
      cagctcagg tgtcagttg aagccaccc ccatctctt 
      cttgccgcc gccgcaggt aacaacaac gtgaaaaag 
      aacgaaaac aatagacaa actcagtac agttcagtg 
      acttcgtct cgaggttca actaagacg tttacgccc 
      ccattgcct gccagtggt aacttccaa atggtgcaa 
      aataatggc gttaaaaac gtaggacag aaggcgagg 
      tcagttcct tatcaacaa cagaggcaa ccaagtcca 
      cctgtggtg gtgcgacac gtcttcaca tcaggacag 
      ggaattcct gtcactatg gctgtgctt ccacaacct 
      cctgcctta agtccagaa gtggttgaa gccccgaat 
      ccgatgggc gccgtcggc caatacatc ctcgtgcag 
      cgcgctggc gtcggcgac caccacaac atcccccgt 
      tcgtccagc gcaccccca tcccaccag cagcagctc 
      ccgacgccg atcaacact ggtggcgtc tcgcagata 
      gtttcagtg ggtacagcg cgcggtcgt ccggccagt 
      gtcgacgcg gaccatccg atccttcaa cagcagcca 
      ccgccccag ccgccacaa tcgaacgac tttattgtt 
      cagtgtccg aatccgggc actcaagcg gtcacgcgc 
      agacagaga ctgccttcc ggtgtagtg tacggcgac 
      gtaagcgtt gagaatcaa atgcataat tatacgatc 
      attggcggc gagatggtt gaccatccg ggggcggcg 
      atgcagccg atgcaaatg atgccgaag cggacgacg 
      gacaattgc gcgtgcagt ctgaaagcg atggtcgta 
      tgtaaaaag tgcggagcg ttctgtcac gacgactgt 
      attggaccg aatagactt tgtcgtacg tgttttata 
      cggtaggtt ggattgtgt gagagatta tttgttggt 
      aacggtcaa tcagggtaa aagcgtaga taacaggtc 
      gcactcaag atacataga agtttatgg tacgtcaac 
      ttaacgtca aaacgagag tacgaaagc agcatagaa 
      tatgaaagc aatcaaatt aaattgaaa cagacggca 
      ccacggtag gcgaaggac tatcagttt tgttgacga 
      tgctgtcag cattctcaa gcaaagtaa aatttacaa 
      taaagaagc ttaaattga taagaaaat aacttgaaa 
      aacgttaat tttctgagt gttgaaggt attagactt 
      tcttgacca gtcatctta atagggaaa attctgtaa 
      aactcagcg gcaaaaagt ctaccattc tttcacgtt 
      accaactta ccttataga agaatagtg ttgaagttt 
      caggcacaa aatagttca gacaaggtt tatagcagc 
      acaaattta ttatctatc tctccctag tacggcccc 
      tacggggat gacaggtca agaaagact tgatacccc 
      caagaggct cagtaaatt aacgttttc caagttatt 
      ttcttatca atttaagca tcttttttg taaatttta 
      cttttgcat gaaaatgtt gacagcact gtcaaccgt 
      ggccgacga aaatgacat ttcgcttca ctcatgtgg 
      ccgtttcac tggacatga aattctgat tcagatcag 
      aaggccttg aagcggccg gcatgtaga gccctctac 
      aggcagaat agcagaata aaaccgcga aattcga
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