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Gene information
General information
TC032990
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013897,TC013896
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 414874..431199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032990
    iBeetle screen iB_05519

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 57.14% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    legs partiallypigmentation/tanning decreased - Number of animals showing the phenotype: 3


    metathoracic leg tarsus irregular - Number of animals showing the phenotype: 2

    Fertility
    mature egg deposition 2 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 2
    iBeetle screen iB_05520

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 5

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 5

    vitellogenic egg chamber structure irregular - Number of animals showing the phenotype: 5

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032990 RA
      MLEPYAPSS IWLVVVGFV IAFILAFGI GANDVANSF 
      GTSVGSKVL TIVQACCLA TVFEVAGAI LIGYKVSDT 
      MRKGILDIK VYNDAEVEL MLGSLSALT SSAAWLMVA 
      TFFKLPISG THSIVGATV GYSLVARGT RGVHWGTLA 
      KIVGSWFIS PVLSGVTSV CILLIIKYL ILSKPDPLE 
      AGLFSLPLF YSFTILVNV FSIVHDGPK LLYMDNIPF 
      WLATTISLS IAFLTMIFI WLVVVPWQR RKIKADLLA 
      EKPTVNFNI GESTDTSPE GSPRKSNRN STALSERQL 
      TVISESTEL QAMENKKTA AKYIFPLQT NDNKNGYMP 
      AKETEIHTR PKTLKLVES TNEVNPTLS PSSSGVPLI 
      INKNTRTQS DQGMCALDI KDNESTLVE NKSVSKLFS 
      FLQVLTAMF GSFAHGGND VSNAIGPLV TLWLIYTEG 
      TVHQKSETP LYILLFGGF GISVGLWLW GRRVIQTIG 
      EDLTTITPS TGFTIEIGA AFTVLLASK IGIPISTTH 
      CKVGSVVFV GYFSSSKKG VDWSLFRNI ISAWLITVP 
      IAALLSAAT MFLLRYIVL D
    CDS sequence
    • >TC032990 RA
      atgttggaa ccctacgcc cccagttcg atatggctt 
      gtggtggta ggattcgta atcgccttc atcctggcc 
      ttcggcatc ggagccaac gacgtcgcc aactccttt 
      gggaccagc gtgggctcc aaagtcctc accatagtc 
      caggcgtgc tgtttggcc acggttttc gaagtggcc 
      ggtgctatt ctcataggt tacaaggtc tcggacacc 
      atgaggaag ggcatcctg gatatcaaa gtgtacaat 
      gacgccgaa gtcgagctt atgttgggc tctctgagc 
      gctttgaca tcgagtgcc gcctggctg atggtcgca 
      acgttcttc aagttgccc atttcaggg actcacagc 
      atcgtgggc gcgacggtg gggtacagt ctcgtcgcc 
      agggggacc aggggcgtg cactggggc acgcttgcc 
      aaaattgtg gggtcctgg tttatctcc ccggtgctg 
      agtggtgta acttcagtc tgcattttg ttaattata 
      aagtacttg attttatcg aaacctgat cctctcgaa 
      gcaggtctc ttctcgttg ccccttttt tacagtttt 
      acgatatta gttaacgtt ttttccatc gtacacgat 
      ggtcctaaa ttgctctac atggacaac ataccgttt 
      tggttggcg actaccata agtctttcc atcgccttt 
      ctcacaatg attttcatc tggttggtg gtagttcct 
      tggcagaga cggaaaatc aaagctgat ttgttagcc 
      gagaaaccg actgttaat ttcaacatt ggcgagtct 
      acagatacg tcacctgag ggcagtcca cgaaaatcg 
      aatcgcaac tcaacggct ttatctgaa cgtcaatta 
      accgtaata tccgaaagt accgaactt caagccatg 
      gaaaataaa aaaacggcc gctaaatac atcttccca 
      ctgcaaacc aatgataac aaaaatggt tacatgccg 
      gctaaagag actgaaatc cacaccaga cctaagact 
      ttaaaactg gtcgaaagt acaaacgaa gtgaaccca 
      actctgtct ccaagctcg agtggagtt cccttaatt 
      attaataaa aatactaga acacaaagt gaccaagga 
      atgtgtgct ttagatatc aaagataac gaaagcact 
      ctcgttgaa aataaaagc gtctccaag ttgttcagt 
      tttttgcaa gttttaact gccatgttt ggtagcttt 
      gctcatgga ggaaatgat gtcagtaac gcaataggc 
      cctttagta actttatgg ctcatctat accgaggga 
      acagtgcac caaaaatcg gagactcct ctctacatc 
      ttgctgttt ggagggttt ggaatttca gtcggattg 
      tggctttgg gggaggaga gttatacaa acgattggg 
      gaagatttg accacgata accccctca actggtttt 
      acgattgaa atcggtgca gcttttaca gtattacta 
      gccagcaaa atcggaata cccatctcg accacccac 
      tgtaaagtc ggatcagtt gttttcgtc ggttatttc 
      agttcttcg aaaaaagga gtagattgg agtctattt 
      aggaacatc atctcagct tggttgata acagtgccc 
      attgcagct ttactctct gctgcaacc atgtttctt 
      ctacgttac attgttttg gattaa
    mRNA sequence
    • >TC032990 RA
      tgacgaaat ccgccgtcg tctccgaca cagggaagc 
      tctaaaagc tgctctcac tccgcatga tgagtgtcc 
      aaaaagcct agccaacaa cttcaccac ctccaatca 
      actcagaag aatgttgga accctacgc ccccagttc 
      gatatggct tgtggtggt aggattcgt aatcgcctt 
      catcctggc cttcggcat cggagccaa cgacgtcgc 
      caactcctt tgggaccag cgtgggctc caaagtcct 
      caccatagt ccaggcgtg ctgtttggc cacggtttt 
      cgaagtggc cggtgctat tctcatagg ttacaaggt 
      ctcggacac catgaggaa gggcatcct ggatatcaa 
      agtgtacaa tgacgccga agtcgagct tatgttggg 
      ctctctgag cgctttgac atcgagtgc cgcctggct 
      gatggtcgc aacgttctt caagttgcc catttcagg 
      gactcacag catcgtggg cgcgacggt ggggtacag 
      tctcgtcgc cagggggac caggggcgt gcactgggg 
      cacgcttgc caaaattgt ggggtcctg gtttatctc 
      cccggtgct gagtggtgt aacttcagt ctgcatttt 
      gttaattat aaagtactt gattttatc gaaacctga 
      tcctctcga agcaggtct cttctcgtt gcccctttt 
      ttacagttt tacgatatt agttaacgt tttttccat 
      cgtacacga tggtcctaa attgctcta catggacaa 
      cataccgtt ttggttggc gactaccat aagtctttc 
      catcgcctt tctcacaat gattttcat ctggttggt 
      ggtagttcc ttggcagag acggaaaat caaagctga 
      tttgttagc cgagaaacc gactgttaa tttcaacat 
      tggcgagtc tacagatac gtcacctga gggcagtcc 
      acgaaaatc gaatcgcaa ctcaacggc tttatctga 
      acgtcaatt aaccgtaat atccgaaag taccgaact 
      tcaagccat ggaaaataa aaaaacggc cgctaaata 
      catcttccc actgcaaac caatgataa caaaaatgg 
      ttacatgcc ggctaaaga gactgaaat ccacaccag 
      acctaagac tttaaaact ggtcgaaag tacaaacga 
      agtgaaccc aactctgtc tccaagctc gagtggagt 
      tcccttaat tattaataa aaatactag aacacaaag 
      tgaccaagg aatgtgtgc tttagatat caaagataa 
      cgaaagcac tctcgttga aaataaaag cgtctccaa 
      gttgttcag ttttttgca agttttaac tgccatgtt 
      tggtagctt tgctcatgg aggaaatga tgtcagtaa 
      cgcaatagg ccctttagt aactttatg gctcatcta 
      taccgaggg aacagtgca ccaaaaatc ggagactcc 
      tctctacat cttgctgtt tggagggtt tggaatttc 
      agtcggatt gtggctttg ggggaggag agttataca 
      aacgattgg ggaagattt gaccacgat aaccccctc 
      aactggttt tacgattga aatcggtgc agcttttac 
      agtattact agccagcaa aatcggaat acccatctc 
      gaccaccca ctgtaaagt cggatcagt tgttttcgt 
      cggttattt cagttcttc gaaaaaagg agtagattg 
      gagtctatt taggaacat catctcagc ttggttgat 
      aacagtgcc cattgcagc tttactctc tgctgcaac 
      catgtttct tctacgtta cattgtttt ggattaaga 
      cactagtag ctaaatcaa cagataata caggatggc 
      gcaaaagta tgttttcaa tttaaactg ggaaaaaca 
      aattaattt gtaaataaa ctagatttt ctggtttaa 
      ggatatact actcggaca cagctttaa aataaattc 
      agtttataa aaatttgct actctatcc gtctcttat 
      aataaaaat ctttttgga attgcaaac aaccccttt 
      gggaaaatg aaaactcca ctacaatca attataagg 
      ttgatgaat ttctagtac aaatatatt tatataaaa 
      ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY