Status
Please wait ...
Gene information
General information
TC032992
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013892
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 315277..330139
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032992
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05518

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection



    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition 2 number 2nd lateral oviduct: 4 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032992 RA
      MTNAAEEKN ETFKTPEYI IPCYKADPE INKCLQRTF 
      NHLRPYLLS GIQDINVPS IDPLKIDKL VMENGQGAL 
      RIRALFHNV TASGGSNYT ITKIKADVV NYNIELGFK 
      LPRVEIKGK YEVNGNVLL FPVRSKGDF WAIFLDVDA 
      AAKIYGKEF KDKNNTRFM KIEKLLIDF RLQKSRFRV 
      RDIINHGNI IGEAMNQFL NSNANEIIA EMKPAANAA 
      IAKHFKGFL NSAFLKLPL KVWLPDA
    CDS sequence
    • >TC032992 RA
      atgacgaat gctgcagaa gaaaaaaat gaaactttc 
      aaaacacct gaatacatc atcccttgc tacaaagca 
      gaccccgaa atcaacaaa tgcctccaa cgcacattc 
      aaccacctt cgaccctac ttattatca ggaatccaa 
      gacataaac gtgcccagt atcgatcct ttaaagatt 
      gataaactt gttatggaa aatggtcaa ggagcctta 
      agaattcgg gccctcttc cacaatgtc acagcttcc 
      ggtggcagc aactacacc atcaccaaa ataaaagcc 
      gatgttgtg aattataat atcgaactc ggtttcaaa 
      ctgccccga gttgaaatc aaaggaaag tatgaagtt 
      aacggaaac gtcctcctg tttccagtg aggtcgaaa 
      ggcgatttt tgggccatt tttcttgat gtcgacgcc 
      gctgcgaaa atctacgga aaggaattt aaagacaaa 
      aacaacact cgatttatg aagattgaa aaattattg 
      atcgatttt agactgcag aaaagtcga tttagagtt 
      agggatata atcaatcat ggcaatatt attggtgag 
      gccatgaat cagtttttg aatagcaac gcgaacgaa 
      atcatcgcc gaaatgaaa ccagcggca aatgcagca 
      attgctaaa catttcaag ggatttttg aacagtgcg 
      ttcttgaaa ttaccattg aaagtctgg ttgcctgac 
      gcgtag
    mRNA sequence
    • >TC032992 RA
      attcattca taggattca tttgagtct ccttccgga 
      cctcttcgg attggtgga acaacttca ggacctcct 
      ctgaaggat tgcactctt tggacctct tcaaaggat 
      acagtcgac gggaccttc tttcgtcgt taaaccaca 
      cctgcccca agacatctt ctgggaacc aagttcacc 
      tttgaacac gggccacgg gacctcttc cgtgacatg 
      ctttctcta aatgctaaa taatgaaca ccgagtaat 
      cacaaataa ctaaaccag tgcagtaag acattgtga 
      taagacgaa aaaatgttg cgattattt acattcatc 
      tcgattgtt ttattattc caaatgacg aatgctgca 
      gaagaaaaa aatgaaact ttcaaaaca cctgaatac 
      atcatccct tgctacaaa gcagacccc gaaatcaac 
      aaatgcctc caacgcaca ttcaaccac cttcgaccc 
      tacttatta tcaggaatc caagacata aacgtgccc 
      agtatcgat cctttaaag attgataaa cttgttatg 
      gaaaatggt caaggagcc ttaagaatt cgggccctc 
      ttccacaat gtcacagct tccggtggc agcaactac 
      accatcacc aaaataaaa gccgatgtt gtgaattat 
      aatatcgaa ctcggtttc aaactgccc cgagttgaa 
      atcaaagga aagtatgaa gttaacgga aacgtcctc 
      ctgtttcca gtgaggtcg aaaggcgat ttttgggcc 
      atttttctt gatgtcgac gccgctgcg aaaatctac 
      ggaaaggaa tttaaagac aaaaacaac actcgattt 
      atgaagatt gaaaaatta ttgatcgat tttagactg 
      cagaaaagt cgatttaga gttagggat ataatcaat 
      catggcaat attattggt gaggccatg aatcagttt 
      ttgaatagc aacgcgaac gaaatcatc gccgaaatg 
      aaaccagcg gcaaatgca gcaattgct aaacatttc 
      aagggattt ttgaacagt gcgttcttg aaattacca 
      ttgaaagtc tggttgcct gacgcgtag gcgctttat 
      cgtagttaa ttaattgat ctattggca attttttaa 
      aaatcgcca tttagaacg aatctctct actattgta 
      tatagaatt tttgtaaaa tatgtttaa atgttcgtt 
      tttggacaa attgtttat aataaaaat aaaatactt 
      caagtgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY