Status
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Gene information
General information
TC013868 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2655233..2657637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013868
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05514

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013868 RA
      MRNLEDFPV PIDGEVTLC EINGRMQCM NHEFANVST 
      FTTAVDKHP KVHITRDLL HLPNLGDEG RALFLPTDV 
      PFLKQLTQV YAEQGLKEV LHTATLHQH LLISQSARF 
      LLAVWRYLR KARLILNPS LEYHSCELI DELSQTRNW 
      ANNTIKCIA WHLHNSRLA VATCDDSVR IYCNDSSFV 
      PLLRCKQQR NVTCLAWRP MSLTEIAVG HENGIIIWH 
      VDFNSLVTR PSVSNSIML QRVDHKPVM SLAWSPRGN 
      LLVSAAALD SNILVWDVE LNKTSVLKG SRDSGNILL 
      KWSPAKDKL LSVSNGIVF RIWDCQHWE NERWTVPSG 
      RVQAACWTS CGTSLLFAT STEPVIYGL NMKSDHVFS 
      SNSESSIDH AVPLFDLSK IDLEGIAVG GIVQSMEAD 
      PKGNHLAVF FKDTSCVAL FSVIKSPLL QLIPSSLIV 
      GLAAEVPST IAFQYNFKP GACLSIAWS SGHLQHFPI 
      IYTDLTNDF NASQYVQSQ IRSLNNSR
    CDS sequence
    • >TC013868 RA
      atgagaaat ttggaagat ttcccggtt cccatcgac 
      ggcgaggtg actttatgt gagataaac ggccgaatg 
      caatgcatg aatcacgaa tttgccaac gtttcaacc 
      ttcacgact gctgttgac aaacacccg aaggtgcac 
      ataactcgt gatttgctg catctgccg aacttgggg 
      gacgagggt cgggcgttg tttttgccg actgatgtg 
      ccgtttttg aaacaactc acacaggtg tacgctgag 
      cagggcttg aaggaagtt ttacatacc gccactttg 
      catcaacat ttgttaata tcacagagc gcccgcttt 
      ttgttggca gtctggcgg tatttgcgt aaggcgagg 
      ttgattcta aacccgagt ttggagtac cacagttgt 
      gaattgata gatgagttg agccaaacg agaaattgg 
      gctaataac acgatcaaa tgcattgcg tggcatttg 
      cacaattcg cgactagct gtggcgacg tgtgacgac 
      agcgttaga atttactgt aatgatagt agttttgtt 
      cccttgctg aggtgtaag cagcaaagg aatgtgacg 
      tgtttggcc tggaggccc atgtctttg acggaaatt 
      gcagtaggg catgaaaat ggcattata atatggcac 
      gttgatttc aactcattg gtgactagg ccttcagta 
      agcaacagt attatgtta caaagggtg gaccacaaa 
      cccgtcatg agtctagcc tggagcccc cgaggcaat 
      ctcttagtc agtgccgca gccctggac tcaaacatc 
      ctcgtctgg gacgttgaa ttgaacaaa accagtgtt 
      ttgaaaggt tccagagac tcgggaaat attctactg 
      aaatggtct ccagccaaa gacaaatta ttgagtgtt 
      tcgaacgga atcgtattt aggatttgg gattgccag 
      cactgggaa aacgagcgt tggacggtg ccttcaggc 
      cgcgtccaa gcagcttgt tggacgagt tgtggcaca 
      agtttgctc tttgccaca agtacagag ccagttatt 
      tatggactg aacatgaaa agtgaccat gtgtttagc 
      agcaattcc gagagttct attgaccac gctgtgcct 
      ttatttgac ctctctaaa attgatttg gagggaatt 
      gcagttggc gggattgtt cagtcaatg gaagctgat 
      ccaaaagga aaccacttg gctgtgttt tttaaagat 
      acgagttgt gttgcgctt tttagtgta ataaagtcg 
      ccactactc cagctaata ccaagctcg ttgattgtc 
      ggtttggct gctgaggtg ccaagtaca atagcgttt 
      caatataat ttcaaacct ggagcttgt ctgtccatt 
      gcctggtct agtggacac ctccaacac ttcccaatt 
      atttacact gatttgacc aatgacttc aatgcaagc 
      cagtatgtc cagtctcaa atcaggtct ttaaataac 
      tctagataa
    mRNA sequence
    • >TC013868 RA
      cagttggtg gttgagttt gaaaaccgg taaaaagtg 
      aacgaaaat gagaaattt ggaagattt cccggttcc 
      catcgacgg cgaggtgac tttatgtga gataaacgg 
      ccgaatgca atgcatgaa tcacgaatt tgccaacgt 
      ttcaacctt cacgactgc tgttgacaa acacccgaa 
      ggtgcacat aactcgtga tttgctgca tctgccgaa 
      cttggggga cgagggtcg ggcgttgtt tttgccgac 
      tgatgtgcc gtttttgaa acaactcac acaggtgta 
      cgctgagca gggcttgaa ggaagtttt acataccgc 
      cactttgca tcaacattt gttaatatc acagagcgc 
      ccgcttttt gttggcagt ctggcggta tttgcgtaa 
      ggcgaggtt gattctaaa cccgagttt ggagtacca 
      cagttgtga attgataga tgagttgag ccaaacgag 
      aaattgggc taataacac gatcaaatg cattgcgtg 
      gcatttgca caattcgcg actagctgt ggcgacgtg 
      tgacgacag cgttagaat ttactgtaa tgatagtag 
      ttttgttcc cttgctgag gtgtaagca gcaaaggaa 
      tgtgacgtg tttggcctg gaggcccat gtctttgac 
      ggaaattgc agtagggca tgaaaatgg cattataat 
      atggcacgt tgatttcaa ctcattggt gactaggcc 
      ttcagtaag caacagtat tatgttaca aagggtgga 
      ccacaaacc cgtcatgag tctagcctg gagcccccg 
      aggcaatct cttagtcag tgccgcagc cctggactc 
      aaacatcct cgtctggga cgttgaatt gaacaaaac 
      cagtgtttt gaaaggttc cagagactc gggaaatat 
      tctactgaa atggtctcc agccaaaga caaattatt 
      gagtgtttc gaacggaat cgtatttag gatttggga 
      ttgccagca ctgggaaaa cgagcgttg gacggtgcc 
      ttcaggccg cgtccaagc agcttgttg gacgagttg 
      tggcacaag tttgctctt tgccacaag tacagagcc 
      agttattta tggactgaa catgaaaag tgaccatgt 
      gtttagcag caattccga gagttctat tgaccacgc 
      tgtgccttt atttgacct ctctaaaat tgatttgga 
      gggaattgc agttggcgg gattgttca gtcaatgga 
      agctgatcc aaaaggaaa ccacttggc tgtgttttt 
      taaagatac gagttgtgt tgcgctttt tagtgtaat 
      aaagtcgcc actactcca gctaatacc aagctcgtt 
      gattgtcgg tttggctgc tgaggtgcc aagtacaat 
      agcgtttca atataattt caaacctgg agcttgtct 
      gtccattgc ctggtctag tggacacct ccaacactt 
      cccaattat ttacactga tttgaccaa tgacttcaa 
      tgcaagcca gtatgtcca gtctcaaat caggtcttt 
      aaataactc tagataatg ttttctaac gaattgtat 
      ttttgtaag aattaaata aaattttat cactgtctt 
      ggcctctta ttacttgtg taggtcccg gggtcagac 
      tgtcgtttt tctgcaaat catacgtta aataaatag 
      tgaaataaa tcagttgta gacttacta attttattt 
      tctgctgca tttgacgcc gatacgaat gttctttgc 
      aatttcgtc tttttaact tctctgtat ttcttggca 
      atgtcttta cttaattcg gccactctc accatcaaa 
      ggggctgga taactgaag cattttgct taagttaat 
      actttcttt agtttgatt ataatataa ctcacgtgc 
      accccataa attccatat ttgtaaaag ctgtagcta 
      aagcaacta agtagtctt ctggaaatt ttcctttat 
      gggctttca aaaattcct tccgcagct gccagtgct 
      cctcaatgt cggtatcat ttcggtact tctctatat 
      cccaatcag tggggtatg actgcgtca ttttgccaa 
      tttttgttt cttctgact aatttgaat aaatgtggg 
      taaacataa ccaaaattt tgacaatac caaaaagtt 
      gacacttac acagctgcc aagtgccaa cctatctgc 
      tttgaaatc tagcaattt ttaattatc accactagg 
      aataattca gttgatgga atagcacga agatatgtt 
      tgcaaagtg ataaataaa ttacacaac tagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY