Status
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Gene information
General information
TC013858 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2789594..2794608
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013858
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05513

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment:
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva dorsalirregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva ventralirregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages distal decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs dorsoventral-axis irregular -
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013858 RA
      MAAVLKKRA LAEFTQTIQ ETVPFQPIK KLKLVKKPS 
      TGTGNSSLA LISCLEGCK SSSEALRTL LRVSDTFEL 
      EASDIPEAV KKLSDHFKN EPESAVRVK ILSLLCDIG 
      HLPNADVVS IIDETILLL KNDHSHKVI AQGMNTVLK 
      LGKLVSDNV IALHQKLVE VAKSYLKDV SHAVKRKCL 
      EIIGVHTPV CTGAEAEKL MVLISSYFN HDDARVRSQ 
      AFSTIITLH ERNFKINPD IYIDVCDAL KDDYEIVRQ 
      VVLKLVCLL GNTFPENEV VLPGSEHEI RLIDDAFGK 
      VCNSISDLS IHVRTLAAK LLGTMKQVS AKFLNQTLD 
      KKLMSNMRR KRTAHELAW ENVTSGEWA SGKKWADDA 
      PREILDADS INLMSSGAC GAFVHGLED EFMEVRSAA 
      VESLCELSI RNPQFANLS LDFLVDMFN DEIEDVRLK 
      AIDSLRRIS EHIILRDDQ LETILGALE DFSQDVREG 
      LHHMLASCK MSTTAGLKM CVEKLLDNL KKYPQDKRS 
      TYRCLQRVG AQHPELVLP LVPQLLNIH PFFDTAEPD 
      VENPQYICV LILILNAAK FSPTIIPLL EPHTLRHYA 
      YISDTMPSL VPVLSLPES SCNTISRPQ TVPESLEFL 
      NNVISNLDL AENTLRVQT TLLQTAKEH LNRLSEIDS 
      SVAGTAQFT SLYIGAQLI MSQILEKGY WLNPGTLAT 
      QQANNLKTN ITNLLEYCL KLQFFFVGL SPVEECAVK 
      MFRLRALAL NLVYIVKGS NSSALAPCH HFLTIVEDM 
      QRDLQVAGL DPDAFTSAV FKELSVLEE PKPGTVSRI 
      LIPILSEAR LGKIPRPNV QIRMSSAEI VEPNGQTDT 
      SLKFTAGLV MSVHFDAEL RHLIDPSRV RLKVKYPDQ 
      KTQIILPRP AHMKPLFVD GEDAQAGHN VRLLTSVLI 
      SHQVWSEAC NVEINVALA IPEADIGKR KANVSATPT 
      LIDLCKPVK VSVAPKPIK RTL
    CDS sequence
    • >TC013858 RA
      atggctgca gtgttgaaa aaacgcgct ttggccgaa 
      ttcacccaa acaatccag gaaactgtt cctttccaa 
      ccgattaaa aaactcaaa cttgtcaag aagccttcc 
      acaggcacc gggaactcg tctttggca cttattagt 
      tgtcttgaa ggttgcaaa agcagtagc gaagcccta 
      cgaactttg cttcgtgtc tctgatact ttcgagctt 
      gaggcttca gatattccc gaagcagtt aaaaaactg 
      agcgatcat tttaagaat gagccagag tcggccgtt 
      agagtaaaa attttgtca cttttgtgc gacattggg 
      catctaccc aacgctgat gttgtgtcc attattgac 
      gagacgatt ttgttgctt aaaaatgac cattcacat 
      aaagttatc gctcagggg atgaatacc gtgcttaaa 
      ttagggaag ctggtctct gataatgtc atcgctttg 
      catcagaaa ctggtagaa gtggcgaaa agttacttg 
      aaagatgtc agtcatgct gtcaagagg aaatgtcta 
      gagatcatt ggcgtgcat acgccggtt tgtacgggg 
      gcagaggcg gagaaatta atggttttg attagttcg 
      tactttaac cacgacgac gccagggtg cgtagtcag 
      gctttttcg acgattatc accctacat gagcgcaat 
      tttaaaata aatccggac atttatata gatgtttgc 
      gatgcctta aaggacgac tacgaaatt gtccgtcaa 
      gtcgttctc aagttagtg tgtcttttg ggcaacacg 
      tttccggaa aatgaggtt gtacttccg ggaagtgaa 
      cacgaaatc cgtctcata gacgacgct ttcggtaaa 
      gtttgcaac tcgatttct gatttatca atccatgtt 
      cgaaccttg gcggctaaa ctattggga acgatgaaa 
      caagtcagt gcgaaattt ctcaaccaa actcttgat 
      aaaaaatta atgtctaat atgaggcgc aaaaggacg 
      gctcatgag ttggcttgg gaaaatgta acgagtgga 
      gaatgggcg agtgggaaa aaatgggcc gatgacgcc 
      cccagggaa atcctcgac gctgacagc atcaatttg 
      atgagcagt ggggcgtgt ggggccttc gtgcacggc 
      ttggaggac gagtttatg gaggtgcgt tccgccgcc 
      gtcgaatca ctctgcgag ctctccatt cgcaacccc 
      caattcgca aatttatcc ctcgacttc ctggtcgat 
      atgttcaac gacgaaatc gaggatgtg agactcaaa 
      gccattgat agtttacgc cgaatatcc gaacacatt 
      attctaaga gacgaccag ttggagacc atcttaggg 
      gcccttgag gacttttcg caggatgtc cgagagggc 
      ctccaccac atgttggca tcgtgtaaa atgtcaaca 
      acagccggg ctcaaaatg tgcgttgaa aaactatta 
      gataatctc aaaaaatat ccgcaagat aagcgatcg 
      acctatcgg tgtttacaa cgggtgggg gcacagcac 
      cccgaattg gtgctccct ctagtgcca caattgtta 
      aacatacac ccgtttttc gatactgct gaaccggac 
      gtggaaaat ccgcaatat atttgcgtc ctgattttg 
      attttaaat gcggcaaag ttttcacct actataatc 
      ccacttctg gaaccccac accttacgt cattatgcg 
      tacataagt gacacaatg ccgtcccta gttccggtt 
      ttaagtctc ccggaatcg agttgtaac acaattagc 
      agaccacaa acggtcccg gaaagtctg gagtttctt 
      aacaatgtt ataagcaac ttagatttg gccgaaaat 
      acattacga gttcagacg acactttta caaaccgct 
      aaagaacac ttgaaccgt ttgagtgaa attgactcg 
      tcggtcgca gggacggct caattcaca tcgctttac 
      attggggcc caattaata atgtcccag atattggaa 
      aagggttat tggttaaac ccgggtact ttggccacc 
      caacaggcc aacaacctc aaaactaac ataacaaac 
      cttctggag tattgtctc aagttgcag tttttcttc 
      gtagggctt agccctgtg gaggaatgt gccgtgaaa 
      atgttccgc ctgcgggcc ctagccctt aatttagtc 
      tacatagtt aaagggagc aactcctca gctttagcc 
      ccctgccac cacttcctc accatcgtc gaagacatg 
      cagagggac ctacaagtt gcaggtcta gaccctgac 
      gcgttcacg tcagccgtt ttcaaagaa ctatcagtc 
      ttggaggaa cccaaaccg ggaacagtg tcccgaatt 
      ttaatcccg atcctctcg gaagcccga ttgggtaaa 
      atcccgcgt cccaacgtc caaattcgg atgagttcg 
      gccgagatc gtggaaccc aacggccag actgacacc 
      tcgttgaaa ttcaccgcg gggttggtg atgtcggtg 
      cattttgat gccgagtta agacacttg attgaccct 
      tcaagggtc aggttgaaa gtgaaatat ccggatcag 
      aaaacgcaa attattcta ccaaggccg gctcacatg 
      aaaccgttg tttgttgac ggggaggat gcgcaagcg 
      gggcataat gtgaggctg cttacgtcg gtgctgatt 
      tcgcaccaa gtgtggtca gaggcgtgc aacgtggaa 
      attaatgtg gccttggcc attccggaa gcggatatc 
      gggaaacgg aaggccaat gtgagcgcc acgccgacc 
      ttgattgac ctatgcaag ccggtgaag gtcagcgtc 
      gcacccaaa ccgatcaaa aggacccta taa
    mRNA sequence
    • >TC013858 RA
      aacaaaccg tcattcggt ccaacaaca aaatagcat 
      taattacct aattggaca aaacaataa tttagaatt 
      aatgccaga aaccgacaa atgcggtaa attaatacc 
      tttaccgct cataaataa aaaaaatca aaaaaccaa 
      attttatgt aattgtcac caggtggcg gttgcgtaa 
      ctttttgac atttgacaa caatcacaa aataaaatg 
      tttaaaaaa cgctaaaat cggttaatt accacataa 
      acacgatgg ctgcagtgt tgaaaaaac gcgctttgg 
      ccgaattca cccaaacaa tccaggaaa ctgttcctt 
      tccaaccga ttaaaaaac tcaaacttg tcaagaagc 
      cttccacag gcaccggga actcgtctt tggcactta 
      ttagttgtc ttgaaggtt gcaaaagca gtagcgaag 
      ccctacgaa ctttgcttc gtgtctctg atactttcg 
      agcttgagg cttcagata ttcccgaag cagttaaaa 
      aactgagcg atcatttta agaatgagc cagagtcgg 
      ccgttagag taaaaattt tgtcacttt tgtgcgaca 
      ttgggcatc tacccaacg ctgatgttg tgtccatta 
      ttgacgaga cgattttgt tgcttaaaa atgaccatt 
      cacataaag ttatcgctc aggggatga ataccgtgc 
      ttaaattag ggaagctgg tctctgata atgtcatcg 
      ctttgcatc agaaactgg tagaagtgg cgaaaagtt 
      acttgaaag atgtcagtc atgctgtca agaggaaat 
      gtctagaga tcattggcg tgcatacgc cggtttgta 
      cgggggcag aggcggaga aattaatgg ttttgatta 
      gttcgtact ttaaccacg acgacgcca gggtgcgta 
      gtcaggctt tttcgacga ttatcaccc tacatgagc 
      gcaatttta aaataaatc cggacattt atatagatg 
      tttgcgatg ccttaaagg acgactacg aaattgtcc 
      gtcaagtcg ttctcaagt tagtgtgtc ttttgggca 
      acacgtttc cggaaaatg aggttgtac ttccgggaa 
      gtgaacacg aaatccgtc tcatagacg acgctttcg 
      gtaaagttt gcaactcga tttctgatt tatcaatcc 
      atgttcgaa ccttggcgg ctaaactat tgggaacga 
      tgaaacaag tcagtgcga aatttctca accaaactc 
      ttgataaaa aattaatgt ctaatatga ggcgcaaaa 
      ggacggctc atgagttgg cttgggaaa atgtaacga 
      gtggagaat gggcgagtg ggaaaaaat gggccgatg 
      acgccccca gggaaatcc tcgacgctg acagcatca 
      atttgatga gcagtgggg cgtgtgggg ccttcgtgc 
      acggcttgg aggacgagt ttatggagg tgcgttccg 
      ccgccgtcg aatcactct gcgagctct ccattcgca 
      acccccaat tcgcaaatt tatccctcg acttcctgg 
      tcgatatgt tcaacgacg aaatcgagg atgtgagac 
      tcaaagcca ttgatagtt tacgccgaa tatccgaac 
      acattattc taagagacg accagttgg agaccatct 
      taggggccc ttgaggact tttcgcagg atgtccgag 
      agggcctcc accacatgt tggcatcgt gtaaaatgt 
      caacaacag ccgggctca aaatgtgcg ttgaaaaac 
      tattagata atctcaaaa aatatccgc aagataagc 
      gatcgacct atcggtgtt tacaacggg tgggggcac 
      agcaccccg aattggtgc tccctctag tgccacaat 
      tgttaaaca tacacccgt ttttcgata ctgctgaac 
      cggacgtgg aaaatccgc aatatattt gcgtcctga 
      ttttgattt taaatgcgg caaagtttt cacctacta 
      taatcccac ttctggaac cccacacct tacgtcatt 
      atgcgtaca taagtgaca caatgccgt ccctagttc 
      cggttttaa gtctcccgg aatcgagtt gtaacacaa 
      ttagcagac cacaaacgg tcccggaaa gtctggagt 
      ttcttaaca atgttataa gcaacttag atttggccg 
      aaaatacat tacgagttc agacgacac ttttacaaa 
      ccgctaaag aacacttga accgtttga gtgaaattg 
      actcgtcgg tcgcaggga cggctcaat tcacatcgc 
      tttacattg gggcccaat taataatgt cccagatat 
      tggaaaagg gttattggt taaacccgg gtactttgg 
      ccacccaac aggccaaca acctcaaaa ctaacataa 
      caaaccttc tggagtatt gtctcaagt tgcagtttt 
      tcttcgtag ggcttagcc ctgtggagg aatgtgccg 
      tgaaaatgt tccgcctgc gggccctag cccttaatt 
      tagtctaca tagttaaag ggagcaact cctcagctt 
      tagccccct gccaccact tcctcacca tcgtcgaag 
      acatgcaga gggacctac aagttgcag gtctagacc 
      ctgacgcgt tcacgtcag ccgttttca aagaactat 
      cagtcttgg aggaaccca aaccgggaa cagtgtccc 
      gaattttaa tcccgatcc tctcggaag cccgattgg 
      gtaaaatcc cgcgtccca acgtccaaa ttcggatga 
      gttcggccg agatcgtgg aacccaacg gccagactg 
      acacctcgt tgaaattca ccgcggggt tggtgatgt 
      cggtgcatt ttgatgccg agttaagac acttgattg 
      acccttcaa gggtcaggt tgaaagtga aatatccgg 
      atcagaaaa cgcaaatta ttctaccaa ggccggctc 
      acatgaaac cgttgtttg ttgacgggg aggatgcgc 
      aagcggggc ataatgtga ggctgctta cgtcggtgc 
      tgatttcgc accaagtgt ggtcagagg cgtgcaacg 
      tggaaatta atgtggcct tggccattc cggaagcgg 
      atatcggga aacggaagg ccaatgtga gcgccacgc 
      cgaccttga ttgacctat gcaagccgg tgaaggtca 
      gcgtcgcac ccaaaccga tcaaaagga ccctataat 
      ttttgtaaa tattttttg taataataa ataataata 
      atttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY