Status
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Gene information
General information
TC033027
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013848,TC013849
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3060654..3087599
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033027
    iBeetle screen iB_05512

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    No observeable cuticle phenotype found.

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033027 RA
      MTYITATLT IIITLRCAL ADYAHTYVR ENTTILTLK 
      QGYLQGVVV AFRTNRNLP PVEQYKGIP YAVPPVGDL 
      RFMPPGSAP GFGRGVKYA NRFGPVCPQ KFPDTAKMT 
      PERRAEFLR LQQFLGHQS EDCLYLNIY APYRGNSGS 
      YRRYPVMVF IHGESFEWN SGNPYDGSV LAAYGKVIV 
      VTLNYRLGV LGFLKVGSG DYLKSNFGL VDQIAALLW 
      VKENIAEFG GDPDTVTLF GHGTGAVCA NLLMLSPVI 
      QQEHNKTLF QRAILMGGS ALSDWALVS KPLHVTFQV 
      ANALNCQFG KDFADCLRR RRLDEIMTA AEVSSPYQT 
      VFGPVVDAV VLPNEPKEL MTTYSDIFR RFELMYGVT 
      ELESIHLLP ADALKLGML EKDRDQALR RYLESRCEI 
      KPDWCVTRT LAEYTQLDF MEDESFGGY RDEGFDKAS 
      AARDALLDI LSDARTVAP MVQMGQYHS ALNRGSFFY 
      VFTHKTMSK EYIRDKSYT GEELPYVFG VPLGGSRFH 
      FTDYYTEKE RLFSEVMMT YFSNFAYTG DPKMPRKNE 
      YYTLSINDW ISFDVDWPP FDSDQQRYL QLGIPPYTM 
      SHYRHDKMR FWNEAVPDL TTNITTPLL KKPMQTPPI 
      SPPRKTPPW ILTHPPRTT TYVDKYENF HKPYYGVVR 
      VTGTSPPDN IMNNVVYGT EIKSPSPVS EPESKGIST 
      TVTIAMCSI FLLSNLCLF VVLYLMCYR KRKRNGQKT 
      DENPVAPDE ETGNKLMDG CNLMKIVSK SERSEDTYE 
      AVKSHQSGG RFKVARQMS SSTIDAHTK VRDWITNEI 
      VYKYSPGIL RRPRQNQDK SQESKVQNE EKNSTLGRS 
      PTRPVSPVL AEEPPKTRP PLVKSSSIP VQKSRVEKV 
      SVAVDATPS GRGPSVLAQ QPIELTKSL DRPNFDTPL 
      RRSLTLEDF TIIQPKQEL RKSTTSINL KFPQPDSTV 
      VRIEHAHSK SDPVQDFTP RKLLTFDPN SDVNVTSRE 
      EEVKVPLTP EESLMTIKR RNFPKVLPD YPSRDALAK 
      KRRSMPVSH LVFSAIPEL PRKPGKSFG RLPPAPPLR 
      TTSTLTRKA PNQTPVCQS APMLAQEPP SSPEPEVAC 
      NNLYVGPLI PKLHKTELS SSESSKLNT QPIYDKLRV 
      ARGSEKTTK GIAKTIITT DPNQPIKKV EPKVIIKPT 
      MARNVSDPK KHIPRVVLR EDEEKKPSL IPTLVKGKE 
      SSSEESTPS EESDTGTVV KRL
    CDS sequence
    • >TC033027 RA
      atgacgtac atcactgcc acccttacc ataatcatt 
      acgttacgc tgtgccctt gcggactac gcccacacc 
      tacgtaagg gagaacacg accatcctg accctgaag 
      cagggctac ctgcagggc gtcgtcgtg gcgttccgc 
      accaaccga aacctcccc cctgtggaa cagtacaag 
      gggattccc tacgcggtg ccccccgtg ggggacctc 
      cggtttatg cctccgggg agcgccccc ggattcggg 
      cgcggagtg aaatacgcg aaccgcttc gggccggtg 
      tgtccccaa aaattccca gacacggcg aagatgact 
      ccggaacgg agggccgag tttttgaga ctgcaacag 
      tttttgggg catcagagc gaggattgc ctctatttg 
      aacatttac gcgccgtat cgaggtaat tccgggagt 
      taccggagg tatccggtg atggttttt attcatgga 
      gagtcgttc gaatggaat tccgggaat ccttacgac 
      ggcagcgtt ttggcagcg tatgggaaa gtgatcgtg 
      gttacgctg aactataga ttgggtgtt ttaggcttc 
      ctcaaagtg ggcagcgga gactacttg aagagcaac 
      ttcggatta gtggatcag atagcggcg ctgctctgg 
      gtcaaggag aacatcgcc gagtttggc ggtgatccc 
      gacactgtt acgttgttc ggacacgga acgggcgcg 
      gtctgcgcc aacttactc atgctcagt ccggtcatc 
      cagcaggaa cacaacaaa actctcttc caacgggcg 
      attctgatg gggggctcg gccttgtcg gactgggcc 
      ttggtcagc aagcccctc cacgtcact ttccaggtg 
      gccaatgcc ctcaactgc caattcggc aaagacttc 
      gctgactgt ctccgtcga cggaggctc gacgagatt 
      atgacagcc gctgaagtc tcatctccg tatcaaacc 
      gtcttcgga ccagtcgtc gacgctgtg gtcctccca 
      aacgagccc aaggaactc atgacgact tacagcgac 
      attttccga aggtttgag ctaatgtac ggcgtgact 
      gagctcgag agcatccac ctcctccca gccgacgct 
      ctcaaactg ggaatgctg gagaaggat cgtgaccag 
      gcattgagg aggtatctg gagtcgcgc tgcgagatc 
      aagccggat tggtgcgtg acacgcacg ctggctgag 
      tacacacaa ctggatttc atggaagat gagagtttt 
      gggggctac agagacgag ggctttgac aaggcgtcg 
      gcggcgcgg gacgccctc ctggacatc ttgtcggac 
      gctcggacg gtggcgccc atggtgcag atggggcag 
      taccactcg gccctcaac aggggctcc ttcttctac 
      gtcttcacg cacaagacc atgtccaag gaatatatc 
      agagacaag agctacacc ggggaagag ttgccatac 
      gtgtttgga gttcctttg ggaggatcc aggttccac 
      ttcactgat tattacacc gagaaagag cgacttttc 
      tccgaagta atgatgacc tacttctcg aattttgca 
      tacacgggt gacccgaaa atgccacga aaaaatgaa 
      tattacact ttaagtatc aacgactgg atcagtttt 
      gatgtggac tggccaccg tttgattcc gaccaacag 
      cggtactta caattggga attccccca tacacaatg 
      agccactac agacacgac aaaatgaga ttctggaat 
      gaagccgtt ccggacctc actacaaac atcaccacc 
      ccacttttg aaaaaaccc atgcaaaca cctccaatt 
      tcacctcct cgtaaaaca ccgccatgg atcctcaca 
      caccctcct cgtacaaca acatacgtt gacaaatac 
      gagaatttc cacaaaccg tattatggc gttgtacga 
      gtcactggt acttctccg cctgacaat atcatgaat 
      aacgtggtc tatggtacc gaaatcaag tcaccaagt 
      cccgtctcg gaacctgag agtaaggga atttcgacc 
      actgtgact atagcaatg tgttcgatt ttcctcctc 
      tccaatctc tgtctcttt gtggttttg tatctaatg 
      tgttatcga aaacggaag cgaaacggg caaaaaacc 
      gacgaaaat cccgtcgct ccggacgaa gaaacgggg 
      aataaactc atggacggg tgcaattta atgaagatt 
      gtgtcaaaa tcggaaaga agtgaagac acttatgaa 
      gcagttaaa tcgcaccaa agtggcggg agattcaaa 
      gtggctcgc caaatgtcg agtagtacc atcgatgcc 
      catacgaaa gtacgcgac tggattacg aacgaaatt 
      gtctacaag tacagtcct ggaattttg cgacggccg 
      aggcaaaat caagacaaa tcgcaagag agtaaagtc 
      cagaacgag gagaaaaat tcgactttg gggcgcagt 
      cccacacgc cctgtgagt ccagtactt gcggaggaa 
      ccgcccaag acccgccct ccgctcgtt aaatcttcc 
      agtattcca gtgcagaaa agtcgagtt gagaaagta 
      tcagttgcc gtagatgct acgccttca ggaagaggg 
      ccaagtgtt ctagcccaa caaccaatc gaattgact 
      aaatcctta gaccggccc aacttcgac acgcctctt 
      cgccggtct ttaactttg gaagatttc accataatt 
      cagccaaag caagagttg aggaaaagt actacaagt 
      atcaattta aagttccct caacctgac tcaactgtg 
      gtccgtatt gaacacgcc cattcgaaa tccgaccca 
      gtgcaggat tttacgcca cggaaatta ctgactttt 
      gatccgaat tccgacgtt aatgtgact agtcgagaa 
      gaggaggtg aaggtgcca ctcactccg gaggagtct 
      ctgatgacg atcaagaga cgaaacttc ccgaaagtc 
      ctcccggat tatccctct cgtgacgca ctggcgaag 
      aagaggcgt tcgatgccc gtctcacat ctggtgttt 
      tctgcaatt cctgaattg ccccggaaa cccggaaaa 
      tcctttggt aggttaccc ccggccccg cctttgaga 
      accacttcg actttgact cggaaagct cctaatcag 
      acgccagtg tgccaatca gccccgatg ctagctcaa 
      gagccgccg tcttcgccg gaaccggaa gttgcttgt 
      aataatttg tacgtgggt cctttgatc cctaagttg 
      cacaagact gaactgagt tcgtccgag agttcaaag 
      ttaaatacg cagccgatt tatgacaag ttgcgagtg 
      gctagaggg agtgaaaag acgactaaa gggattgct 
      aagactatt atcaccact gacccgaat cagccgatt 
      aagaaggtc gaacctaag gttattatc aagccgacg 
      atggcgcgg aatgtgagt gaccccaaa aagcatata 
      ccgagggtc gtgttgcgg gaggacgag gagaagaag 
      ccgtcgtta atcccgacg cttgttaag gggaaagag 
      tcttcgagt gaggagtcg actccttcg gaggagtca 
      gacaccggg acagtggtt aagagattg tga
    mRNA sequence
    • >TC033027 RA
      gcccggcac taatccgcg acacacgtt tcgaatatt 
      tactcatcc aaattatcc gaaaatagc ccagcgtca 
      cgtgcggca ttactgcgt tcgaatttc agtcgcctc 
      aaaccgcat tttcaggca aagcagtgc gtaactcgg 
      catctttca gtgctcaga caaaatgtg attagtgat 
      aagaaagtg ctgaaatgt gtcccaact gagcggaat 
      ttttggcaa aagcccaac gactcaaat atggccact 
      gcttgagcc cactatgac gtacatcac tgccaccct 
      taccataat cattacgtt acgctgtgc ccttgcgga 
      ctacgccca cacctacgt aagggagaa cacgaccat 
      cctgaccct gaagcaggg ctacctgca gggcgtcgt 
      cgtggcgtt ccgcaccaa ccgaaacct cccccctgt 
      ggaacagta caaggggat tccctacgc ggtgccccc 
      cgtggggga cctccggtt tatgcctcc ggggagcgc 
      ccccggatt cgggcgcgg agtgaaata cgcgaaccg 
      cttcgggcc ggtgtgtcc ccaaaaatt cccagacac 
      ggcgaagat gactccgga acggagggc cgagttttt 
      gagactgca acagttttt ggggcatca gagcgagga 
      ttgcctcta tttgaacat ttacgcgcc gtatcgagg 
      taattccgg gagttaccg gaggtatcc ggtgatggt 
      ttttattca tggagagtc gttcgaatg gaattccgg 
      gaatcctta cgacggcag cgttttggc agcgtatgg 
      gaaagtgat cgtggttac gctgaacta tagattggg 
      tgttttagg cttcctcaa agtgggcag cggagacta 
      cttgaagag caacttcgg attagtgga tcagatagc 
      ggcgctgct ctgggtcaa ggagaacat cgccgagtt 
      tggcggtga tcccgacac tgttacgtt gttcggaca 
      cggaacggg cgcggtctg cgccaactt actcatgct 
      cagtccggt catccagca ggaacacaa caaaactct 
      cttccaacg ggcgattct gatgggggg ctcggcctt 
      gtcggactg ggccttggt cagcaagcc cctccacgt 
      cactttcca ggtggccaa tgccctcaa ctgccaatt 
      cggcaaaga cttcgctga ctgtctccg tcgacggag 
      gctcgacga gattatgac agccgctga agtctcatc 
      tccgtatca aaccgtctt cggaccagt cgtcgacgc 
      tgtggtcct cccaaacga gcccaagga actcatgac 
      gacttacag cgacatttt ccgaaggtt tgagctaat 
      gtacggcgt gactgagct cgagagcat ccacctcct 
      cccagccga cgctctcaa actgggaat gctggagaa 
      ggatcgtga ccaggcatt gaggaggta tctggagtc 
      gcgctgcga gatcaagcc ggattggtg cgtgacacg 
      cacgctggc tgagtacac acaactgga tttcatgga 
      agatgagag ttttggggg ctacagaga cgagggctt 
      tgacaaggc gtcggcggc gcgggacgc cctcctgga 
      catcttgtc ggacgctcg gacggtggc gcccatggt 
      gcagatggg gcagtacca ctcggccct caacagggg 
      ctccttctt ctacgtctt cacgcacaa gaccatgtc 
      caaggaata tatcagaga caagagcta caccgggga 
      agagttgcc atacgtgtt tggagttcc tttgggagg 
      atccaggtt ccacttcac tgattatta caccgagaa 
      agagcgact tttctccga agtaatgat gacctactt 
      ctcgaattt tgcatacac gggtgaccc gaaaatgcc 
      acgaaaaaa tgaatatta cactttaag tatcaacga 
      ctggatcag ttttgatgt ggactggcc accgtttga 
      ttccgacca acagcggta cttacaatt gggaattcc 
      cccatacac aatgagcca ctacagaca cgacaaaat 
      gagattctg gaatgaagc cgttccgga cctcactac 
      aaacatcac caccccact tttgaaaaa acccatgca 
      aacacctcc aatttcacc tcctcgtaa aacaccgcc 
      atggatcct cacacaccc tcctcgtac aacaacata 
      cgttgacaa atacgagaa tttccacaa accgtatta 
      tggcgttgt acgagtcac tggtacttc tccgcctga 
      caatatcat gaataacgt ggtctatgg taccgaaat 
      caagtcacc aagtcccgt ctcggaacc tgagagtaa 
      gggaatttc gaccactgt gactatagc aatgtgttc 
      gattttcct cctctccaa tctctgtct ctttgtggt 
      tttgtatct aatgtgtta tcgaaaacg gaagcgaaa 
      cgggcaaaa aaccgacga aaatcccgt cgctccgga 
      cgaagaaac ggggaataa actcatgga cgggtgcaa 
      tttaatgaa gattgtgtc aaaatcgga aagaagtga 
      agacactta tgaagcagt taaatcgca ccaaagtgg 
      cgggagatt caaagtggc tcgccaaat gtcgagtag 
      taccatcga tgcccatac gaaagtacg cgactggat 
      tacgaacga aattgtcta caagtacag tcctggaat 
      tttgcgacg gccgaggca aaatcaaga caaatcgca 
      agagagtaa agtccagaa cgaggagaa aaattcgac 
      tttggggcg cagtcccac acgccctgt gagtccagt 
      acttgcgga ggaaccgcc caagacccg ccctccgct 
      cgttaaatc ttccagtat tccagtgca gaaaagtcg 
      agttgagaa agtatcagt tgccgtaga tgctacgcc 
      ttcaggaag agggccaag tgttctagc ccaacaacc 
      aatcgaatt gactaaatc cttagaccg gcccaactt 
      cgacacgcc tcttcgccg gtctttaac tttggaaga 
      tttcaccat aattcagcc aaagcaaga gttgaggaa 
      aagtactac aagtatcaa tttaaagtt ccctcaacc 
      tgactcaac tgtggtccg tattgaaca cgcccattc 
      gaaatccga cccagtgca ggattttac gccacggaa 
      attactgac ttttgatcc gaattccga cgttaatgt 
      gactagtcg agaagagga ggtgaaggt gccactcac 
      tccggagga gtctctgat gacgatcaa gagacgaaa 
      cttcccgaa agtcctccc ggattatcc ctctcgtga 
      cgcactggc gaagaagag gcgttcgat gcccgtctc 
      acatctggt gttttctgc aattcctga attgccccg 
      gaaacccgg aaaatcctt tggtaggtt acccccggc 
      cccgccttt gagaaccac ttcgacttt gactcggaa 
      agctcctaa tcagacgcc agtgtgcca atcagcccc 
      gatgctagc tcaagagcc gccgtcttc gccggaacc 
      ggaagttgc ttgtaataa tttgtacgt gggtccttt 
      gatccctaa gttgcacaa gactgaact gagttcgtc 
      cgagagttc aaagttaaa tacgcagcc gatttatga 
      caagttgcg agtggctag agggagtga aaagacgac 
      taaagggat tgctaagac tattatcac cactgaccc 
      gaatcagcc gattaagaa ggtcgaacc taaggttat 
      tatcaagcc gacgatggc gcggaatgt gagtgaccc 
      caaaaagca tataccgag ggtcgtgtt gcgggagga 
      cgaggagaa gaagccgtc gttaatccc gacgcttgt 
      taaggggaa agagtcttc gagtgagga gtcgactcc 
      ttcggagga gtcagacac cgggacagt ggttaagag 
      attgtgact tattattac agtatttaa gcagtattt 
      aattgtgtt cgcgacttt tgtatgtgc ataataaaa 
      agatttgta gacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY