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Gene information
General information
TC013795 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3921524..3929359
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013795
    iBeetle screen iB_05500

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013795 RA
      MEVPLIQSR THVSNRVIK TNFKKTMKN FTYLPENST 
      TWGELYNLT NTSDYDYDF QTLNNTGPE STSWALCKE 
      WTPAQHPLF QTANFFFAA AFLVPGSFK QSVLIVRAL 
      LSIGYFFLT LWGGVEVCA PDILLWNSL LVVLNLTHT 
      VLLAWKFLP PTLTLELTD LYLKVFKPL RVSRKHFKE 
      LVREATVMT LERGDIYAV EDVSPADER LSILLKGKL 
      RVTCDDTHL HFINVHQFV DSPEWESNH EQSDDVFQV 
      TITAEEDSV YLCWPRMKL ERVLRHRPI LKVVLDSII 
      GKDITQKLY ALNEHLTGL SDERQRGKR DEMWAKAHN 
      RSMSMDAVN TGTTGLVRS QAYRSTHRK NSLTSNVGS 
      LGKLQNQCW VPLVANQFP ANSPFTANK TTSLKIPEV 
      HSRSLRRKE KDKEVKFEN API
    CDS sequence
    • >TC013795 RA
      atggaggtt cccttgatc cagtcgagg acgcacgtg 
      tccaaccga gtcatcaaa acaaacttc aaaaaaacg 
      atgaaaaat ttcacctat ctaccggag aacagcaca 
      acctggggc gaactttac aacctgacc aacacttcc 
      gactacgat tacgacttc cagactttg aacaacaca 
      ggcccggaa tcgacttca tgggccctt tgcaaggag 
      tggactcct gcacaacat cctctgttt cagacagcg 
      aattttttc ttcgctgct gcgtttcta gtgcctgga 
      agtttcaag cagagcgtc ctcattgtc agggccctg 
      ctctcaatc ggttatttt ttcctgacc ctttggggc 
      ggtgtggaa gtgtgtgcc cctgacatc cttctctgg 
      aactccctt cttgtggtc ctcaacctc acgcatact 
      gtcctactc gcctggaag tttctccct ccaactctc 
      acactcgaa ctcacagat ttgtacctc aaagtcttc 
      aaaccgttg cgagtgagt cgcaaacac ttcaaggaa 
      cttgtgagg gaagccacg gtgatgacg ctcgaacgt 
      ggcgacatt tacgccgtt gaggatgtc agtccggct 
      gacgaaaga ctgtcaatt ttgctcaag ggaaagttg 
      cgtgtgact tgcgatgac actcattta cattttata 
      aatgtgcat cagttcgtc gactccccg gagtgggaa 
      tcgaaccac gaacaatcc gacgatgtt ttccaagtg 
      accataacg gccgaggag gatagtgtt tacttgtgt 
      tggccaagg atgaagcta gaacgggtt ttaaggcac 
      agaccgatc cttaaagtt gtgctagac tcaattatt 
      gggaaagat ataacacag aaactttac gctttgaat 
      gaacatctc accggattg agtgatgaa agacagagg 
      ggaaaaaga gacgaaatg tgggcgaaa gcccacaat 
      agaagtatg tcaatggat gctgtcaat accggaacc 
      accggatta gtccggtcg caagcatat cgtagcaca 
      caccggaaa aattcacta acgtcaaac gtgggttct 
      ctgggcaag ctccagaac cagtgctgg gttccgctc 
      gtagcgaac caattcccg gctaattct cccttcact 
      gcgaacaaa accacttcg ctgaaaatt ccagaggtt 
      cacagtcgc tcgttgcgt cgcaaagaa aaggacaag 
      gaagtcaaa ttcgaaaac gcaccgatt taa
    mRNA sequence
    • >TC013795 RA
      acattagag tgtgttctt taccaaaat catgcaaaa 
      ctttagtaa atcgtgttt aacgtcgct ttccaccgc 
      ttaaaacct atttccggg ccaagccat cgcgttttt 
      atcatttga aattgggat taaaacgca atttcctac 
      cttgtgcaa aatctgggt caaagcagc cgggatcga 
      atttttatt ctcaatcag cgcgtttta tgtgacaca 
      ttaaaattt attgcacat ttcgagccg taattcttg 
      ggcgaaaat tccgtaggt gtagtaaaa gggaatttg 
      tagtgtgtg ttttttccg gcatgtccg tcgacgtaa 
      gcgtctgct atcgattgg cggactaca atgagccag 
      tcgtccaat tcgggcgct tttcctcta atctggaga 
      aaatccccg tgtgttgtt ctgggggac agtagaatg 
      gggcgtgtc gacgagcac accgctcca aatctgtga 
      acacttcac tacaaaaca cgatgaagg cgcccaatt 
      caccaccgg gaaaaacca ccaggaaaa cttgatact 
      atgaagaaa acatggagg ttcccttga tccagtcga 
      ggacgcacg tgtccaacc gagtcatca aaacaaact 
      tcaaaaaaa cgatgaaaa atttcacct atctaccgg 
      agaacagca caacctggg gcgaacttt acaacctga 
      ccaacactt ccgactacg attacgact tccagactt 
      tgaacaaca caggcccgg aatcgactt catgggccc 
      tttgcaagg agtggactc ctgcacaac atcctctgt 
      ttcagacag cgaattttt tcttcgctg ctgcgtttc 
      tagtgcctg gaagtttca agcagagcg tcctcattg 
      tcagggccc tgctctcaa tcggttatt ttttcctga 
      ccctttggg gcggtgtgg aagtgtgtg cccctgaca 
      tccttctct ggaactccc ttcttgtgg tcctcaacc 
      tcacgcata ctgtcctac tcgcctgga agtttctcc 
      ctccaactc tcacactcg aactcacag atttgtacc 
      tcaaagtct tcaaaccgt tgcgagtga gtcgcaaac 
      acttcaagg aacttgtga gggaagcca cggtgatga 
      cgctcgaac gtggcgaca tttacgccg ttgaggatg 
      tcagtccgg ctgacgaaa gactgtcaa ttttgctca 
      agggaaagt tgcgtgtga cttgcgatg acactcatt 
      tacatttta taaatgtgc atcagttcg tcgactccc 
      cggagtggg aatcgaacc acgaacaat ccgacgatg 
      ttttccaag tgaccataa cggccgagg aggatagtg 
      tttacttgt gttggccaa ggatgaagc tagaacggg 
      ttttaaggc acagaccga tccttaaag ttgtgctag 
      actcaatta ttgggaaag atataacac agaaacttt 
      acgctttga atgaacatc tcaccggat tgagtgatg 
      aaagacaga ggggaaaaa gagacgaaa tgtgggcga 
      aagcccaca atagaagta tgtcaatgg atgctgtca 
      ataccggaa ccaccggat tagtccggt cgcaagcat 
      atcgtagca cacaccgga aaaattcac taacgtcaa 
      acgtgggtt ctctgggca agctccaga accagtgct 
      gggttccgc tcgtagcga accaattcc cggctaatt 
      ctcccttca ctgcgaaca aaaccactt cgctgaaaa 
      ttccagagg ttcacagtc gctcgttgc gtcgcaaag 
      aaaaggaca aggaagtca aattcgaaa acgcaccga 
      tttaaaatt gttataccc atgtttggt ttatcattt 
      gcacgtttt tgcttgttc aaaatcatc aaaaaatgt 
      gaccacaaa attgtgcct ttggtgcgc atttaagga 
      agaaacgtt ccaacggtt tttatttcg agcacgcat 
      tcaaccaaa ttatcagtg aattccact atttgcctt 
      ccaatcaaa aaatgagtc aaaaattgt aacgtagcg 
      ttttaacgg agatttaac taccgatag ggtttgcta 
      cgaatgttt tataaaccg ctgtaatat actatgaat 
      gttagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY