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Gene information
General information
TC013794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3932108..3937289
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013794
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05499

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013794 RA
      MPEHTGLQH NEERPHILI VGTADFNNS SPLNNKNFT 
      YNIPSSTWW PWCDYWKDP QNILFQLAN MCFVVAYSS 
      TCSKKGVLF MHCWLILGL MLFSAWAWN IICAPDVFT 
      WNFAFMLMN MAQVFHILY QLRPVKFDP ELEEAYHAL 
      FEPFKLSRL QFKRMVSAE FAQIMSLHA GEAYAVQNL 
      TRTDRLGLL LSGKVNVLS DNQFLHPIL PCEFLDSPE 
      FESSRNTVD DKFKVSIVA ASTCRYLYW QRSALEYLF 
      VKDTYVATV LTTLIARDI ATKLYAMNN KIVTAKGSH 
      LDIRLPSIT SSLTSSGEY KSPIRTKKT EGMCPLSPG 
      TFSNDSRYR SYEKFKENG TLNQNYLSE MEPLTELPS 
      NDDLTSNDV ESWLETSSK YHSCEIVDM D
    CDS sequence
    • >TC013794 RA
      atgcctgaa cacacaggc ctgcaacat aacgaagaa 
      cgcccccat atcctaata gtcggcaca gcagacttt 
      aacaactct tcacctctc aataacaaa aatttcact 
      tataacatc ccttcaagc acttggtgg ccatggtgc 
      gactactgg aaggacccc cagaacatc ctgttccag 
      ctcgccaac atgtgtttc gttgtggct tattcctcc 
      acctgctcc aagaaagga gttctgttc atgcactgt 
      tggctcatc ctgggcttg atgctgttt tcggcttgg 
      gcttggaac ataatttgc gcccctgat gtgttcaca 
      tggaatttc gctttcatg ctaatgaac atggcgcaa 
      gtgttccac attttgtac caactgaga cctgttaag 
      ttcgatccg gagctggag gaggcctat catgcactg 
      tttgaaccg tttaaactt tccaggctg cagtttaag 
      cgaatggtg agcgccgaa tttgctcaa attatgtcg 
      ctgcatgct ggggaggcc tacgcagtg cagaatttg 
      acgagaacg gatcgcttg gggttgctg ctttcagga 
      aaagtgaat gtcttgtcc gataaccag tttttacac 
      ccgattttg ccgtgcgag tttttggat tcgccagaa 
      ttcgaatcg agcaggaac acagtcgat gacaagttc 
      aaagtgtcg atcgtggct gcgagcact tgtcggtac 
      ctatactgg caaaggtct gcccttgag taccttttc 
      gtcaaagac acctacgtg gccactgtt ttgacgact 
      ctaattgct cgcgatatt gccacaaaa ctgtacgct 
      atgaataat aaaatcgtc acagctaag ggttcacac 
      ctcgatata agactgcca agcattacg tcgtctttg 
      acgtcgagt ggagagtac aagtcgcca attcgtacc 
      aaaaagact gaagggatg tgtcctttg tcacctggt 
      actttttcg aatgatagc agataccgt tcgtatgaa 
      aagtttaaa gagaacgga actttgaat caaaattat 
      ctgagcgaa atggaacca ctgactgag cttccttcg 
      aacgatgat ctcacgtcg aatgatgtg gaaagctgg 
      ctggagacc agctccaag tatcacagc tgtgaaatt 
      gtagatatg gactga
    mRNA sequence
    • >TC013794 RA
      catgcctga acacacagg cctgcaaca taacgaaga 
      acgccccca tatcctaat agtcggcac agcagactt 
      taacaactc ttcacctct caataacaa aaatttcac 
      ttataacat cccttcaag cacttggtg gccatggtg 
      cgactactg gaaggaccc ccagaacat cctgttcca 
      gctcgccaa catgtgttt cgttgtggc ttattcctc 
      cacctgctc caagaaagg agttctgtt catgcactg 
      ttggctcat cctgggctt gatgctgtt ttcggcttg 
      ggcttggaa cataatttg cgcccctga tgtgttcac 
      atggaattt cgctttcat gctaatgaa catggcgca 
      agtgttcca cattttgta ccaactgag acctgttaa 
      gttcgatcc ggagctgga ggaggccta tcatgcact 
      gtttgaacc gtttaaact ttccaggct gcagtttaa 
      gcgaatggt gagcgccga atttgctca aattatgtc 
      gctgcatgc tggggaggc ctacgcagt gcagaattt 
      gacgagaac ggatcgctt ggggttgct gctttcagg 
      aaaagtgaa tgtcttgtc cgataacca gtttttaca 
      cccgatttt gccgtgcga gtttttgga ttcgccaga 
      attcgaatc gagcaggaa cacagtcga tgacaagtt 
      caaagtgtc gatcgtggc tgcgagcac ttgtcggta 
      cctatactg gcaaaggtc tgcccttga gtacctttt 
      cgtcaaaga cacctacgt ggccactgt tttgacgac 
      tctaattgc tcgcgatat tgccacaaa actgtacgc 
      tatgaataa taaaatcgt cacagctaa gggttcaca 
      cctcgatat aagactgcc aagcattac gtcgtcttt 
      gacgtcgag tggagagta caagtcgcc aattcgtac 
      caaaaagac tgaagggat gtgtccttt gtcacctgg 
      tactttttc gaatgatag cagataccg ttcgtatga 
      aaagtttaa agagaacgg aactttgaa tcaaaatta 
      tctgagcga aatggaacc actgactga gcttccttc 
      gaacgatga tctcacgtc gaatgatgt ggaaagctg 
      gctggagac cagctccaa gtatcacag ctgtgaaat 
      tgtagatat ggactgagt cttcaaata aaacaatta 
      aggaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY