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Gene information
General information
TC013787 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4041042..4074939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013787
    iBeetle screen iB_05497

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013787 RA
      MEAVIREGA LRLLTSEAS CDVILSCQG HRLMAHKLI 
      LSIASPMLH DLLLQDMTE PSVLIFPDI NSNTMSLIL 
      DYIYTGSVI IYSNTINDF LTAANLLKL HIDVEFLQK 
      ANTLREYKL FEKKEEKPA NDVFSKRNQ RKLPSLVPI 
      SSFMPSKKE RKLVSCVIP SPWMPREMP VLSDPRTCL 
      SSTERANVH QIRDRLEES DPNNNNSQQ HQKNKTNSI 
      LSPSSNSSS ELDLSTSLN KLAFEQKLV PQLNDSLVV 
      NKENDKCVN DSTTAFTCP KKHTNRTLC SRKEEGTEE 
      KNSCHDNEK QKPFKCEDC KKTFSQLRN YKYHRSVHE 
      GTKEFAAKC PECGKNFND RGYLSSHMK IHRDRKEYA 
      CPHCPKRFN QRVAYNMHL RIHTGIKPH ECPTCGKSF 
      SRKMLLKQH QRVHTGERP YSCPECGKT FADRSNMSL 
      HARLHTGVK PYSCNLCSK SFTKKHHLK THMNFHTGL 
      KPYTCQKCG LAFSQSSNM RTHYKKCVL KSNGGPSSA 
      EADSN
    CDS sequence
    • >TC013787 RA
      atggaagca gtgattcgt gaaggcgca cttcgtttg 
      ttgacatct gaggcatcc tgtgatgtt atcctgagc 
      tgccaagga caccggcta atggcccac aagctcatt 
      ttgtcaatt gctagtcct atgctgcat gacttgctg 
      ctgcaagac atgaccgag ccatccgtt ctgattttc 
      cccgacatc aacagcaac accatgtcc ctaatcctg 
      gactacatc tacacagga agcgtcatc atttactca 
      aacacaatc aacgacttc ctcacggct gcaaatctg 
      ttgaaacta cacattgac gtcgagttt ttgcaaaag 
      gcaaacacg ctcagagaa tacaaattg ttcgagaaa 
      aaggaggaa aaaccagcg aacgacgtg ttcagcaaa 
      cgaaaccaa aggaagttg cccagttta gtgcccatt 
      tccagcttc atgcccagc aaaaaggag cggaagttg 
      gtgagctgt gtgataccg agcccatgg atgcccagg 
      gaaatgcct gttctgtcc gacccaaga acctgcctc 
      tcaagcact gaacgagcg aatgttcat caaatacgt 
      gatagactg gaggagagc gatccgaat aacaacaat 
      tcgcaacag caccagaaa aacaaaact aatagcata 
      ctatcacca agctcgaat tcttcctca gaattggac 
      ttatctacc tcattaaat aaattagcc tttgaacaa 
      aaactcgta cctcaatta aatgattcg ttagtggta 
      aataaagaa aatgacaag tgtgttaac gacagtacg 
      actgcgttt acatgtccg aaaaaacac acaaataga 
      acgctttgt agtcggaaa gaagaagga acagaagag 
      aaaaattcg tgtcacgac aacgaaaaa cagaaacct 
      ttcaagtgt gaggattgt aagaaaacg ttcagtcag 
      ttgcgcaat tacaaatac catcgatca gtacacgaa 
      ggaacgaaa gaattcgct gctaaatgt cccgaatgt 
      ggcaaaaac ttcaacgat cggggatat ttaagcagc 
      cacatgaag atacatcgc gaccggaaa gaatatgct 
      tgtccccac tgtccaaaa cggttcaat caaagagtg 
      gcttacaat atgcacctt cgaattcac acaggaata 
      aagccgcac gaatgtccc acatgtggt aaaagcttt 
      tcgagaaaa atgctatta aagcagcac caacgtgtt 
      catacagga gagagaccg tattcttgt ccggaatgt 
      ggaaaaact ttcgcagat cgatctaat atgagctta 
      catgccagg ctacataca ggcgtgaaa ccctattcg 
      tgcaatctg tgctcgaag agctttacg aagaaacat 
      cacttaaaa acacatatg aattttcac acaggactg 
      aaaccatac acttgtcag aaatgcgga ttagcgttc 
      tcgcaaagt agcaatatg aggactcac tacaaaaag 
      tgcgtttta aagagcaac ggaggccct tcttcagcc 
      gaagccgac agcaattga
    mRNA sequence
    • >TC013787 RA
      tgacctgtg ctggtatag gaggcccgg tctcacttg 
      atacagttt tgctttgaa aatgtgctc tagggacta 
      gtagttgag agtaaacag cagtgatta ttcgctcgg 
      agatttcga gttttttga aaaatggaa gcagtgatt 
      cgtgaaggc gcacttcgt ttgttgaca tctgaggca 
      tcctgtgat gttatcctg agctgccaa ggacaccgg 
      ctaatggcc cacaagctc attttgtca attgctagt 
      cctatgctg catgacttg ctgctgcaa gacatgacc 
      gagccatcc gttctgatt ttccccgac atcaacagc 
      aacaccatg tccctaatc ctggactac atctacaca 
      ggaagcgtc atcatttac tcaaacaca atcaacgac 
      ttcctcacg gctgcaaat ctgttgaaa ctacacatt 
      gacgtcgag tttttgcaa aaggcaaac acgctcaga 
      gaatacaaa ttgttcgag aaaaaggag gaaaaacca 
      gcgaacgac gtgttcagc aaacgaaac caaaggaag 
      ttgcccagt ttagtgccc atttccagc ttcatgccc 
      agcaaaaag gagcggaag ttggtgagc tgtgtgata 
      ccgagccca tggatgccc agggaaatg cctgttctg 
      tccgaccca agaacctgc ctctcaagc actgaacga 
      gcgaatgtt catcaaata cgtgataga ctggaggag 
      agcgatccg aataacaac aattcgcaa cagcaccag 
      aaaaacaaa actaatagc atactatca ccaagctcg 
      aattcttcc tcagaattg gacttatct acctcatta 
      aataaatta gcctttgaa caaaaactc gtacctcaa 
      ttaaatgat tcgttagtg gtaaataaa gaaaatgac 
      aagtgtgtt aacgacagt acgactgcg tttacatgt 
      ccgaaaaaa cacacaaat agaacgctt tgtagtcgg 
      aaagaagaa ggaacagaa gagaaaaat tcgtgtcac 
      gacaacgaa aaacagaaa cctttcaag tgtgaggat 
      tgtaagaaa acgttcagt cagttgcgc aattacaaa 
      taccatcga tcagtacac gaaggaacg aaagaattc 
      gctgctaaa tgtcccgaa tgtggcaaa aacttcaac 
      gatcgggga tatttaagc agccacatg aagatacat 
      cgcgaccgg aaagaatat gcttgtccc cactgtcca 
      aaacggttc aatcaaaga gtggcttac aatatgcac 
      cttcgaatt cacacagga ataaagccg cacgaatgt 
      cccacatgt ggtaaaagc ttttcgaga aaaatgcta 
      ttaaagcag caccaacgt gttcataca ggagagaga 
      ccgtattct tgtccggaa tgtggaaaa actttcgca 
      gatcgatct aatatgagc ttacatgcc aggctacat 
      acaggcgtg aaaccctat tcgtgcaat ctgtgctcg 
      aagagcttt acgaagaaa catcactta aaaacacat 
      atgaatttt cacacagga ctgaaacca tacacttgt 
      cagaaatgc ggattagcg ttctcgcaa agtagcaat 
      atgaggact cactacaaa aagtgcgtt ttaaagagc 
      aacggaggc ccttcttca gccgaagcc gacagcaat 
      tgatgcaaa ttatttctt ttaaatttt tagtataat 
      tcttaaagc tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY