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Gene information
General information
TC013781 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4219998..4221639
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013781
    iBeetle screen iB_05496

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013781 RA
      MYAPKKPKL RFLLKLGIL IAALLVVYF TFFHYNSDG 
      SANDATFLL PFKKPKEYN VWLIFTKVS QRSPLRYKF 
      HNLLENILN ISSVPLKFH IIVDNSSKQ LAVGEISDV 
      VSHSNKAIF YKFYDVEAC ARAISDIVD VMTPHFSSK 
      PGTYYSDAL FYISLGLYR IAPPSQRTA LLLDCDLYF 
      KKDIRLVFE EFDKFKPTA LFGLAPELT PVYRHVLQT 
      YKVKKNSTF GEYYHSKAI SSNMQHPRG FQGYNSGVV 
      LINLSAIRK SKEYSQIIR NETVQYLTS KYKFRGHLG 
      DQDFYTLIG FEYPDLIQT INCGFNRQL CVWWREHGY 
      RDVFDFYFK CQHPAVILH GNCNTKIPK
    CDS sequence
    • >TC013781 RA
      atgtacgcc cccaaaaaa cctaaacta cgatttttg 
      ttaaaactt ggaatttta attgctgcg ttattagtt 
      gtttatttt acgtttttt cattacaat tccgatggt 
      tctgctaac gatgcaaca tttttgttg ccattcaaa 
      aagcctaaa gaatacaat gtctggttg atatttact 
      aaagtctct caaaggtcg ccgttaaga tataaattc 
      cacaacttg ttggagaat attttaaac ataagttct 
      gttccttta aagttccat attattgtt gataatagc 
      tcaaagcaa ttagcagta ggtgaaata tcagatgtg 
      gtgtcgcat tcgaataaa gcaatattt tataaattt 
      tatgatgtt gaagcgtgt gcaagagcg attagtgat 
      attgtcgac gttatgacc cctcatttt agttcaaaa 
      ccgggtact tactacagc gacgcactt ttctatatt 
      tctttgggg ctgtatcgg atagcccca ccttcccag 
      cgaactgcc cttctacta gactgtgat ttatacttt 
      aagaaggat attcgatta gtgtttgag gagtttgac 
      aagtttaag ccaactgcg ctttttggt ttggccccc 
      gagctgact ccagtctac cgtcacgtc ctccaaacc 
      tataaagtg aaaaagaac tcaacattc ggagaatat 
      tatcattcc aaggcgata tcgagcaat atgcagcat 
      cctcgtggc ttccagggc tataattcc ggtgttgtt 
      ttgattaat ttaagtgca ataagaaaa tcaaaggaa 
      tattcccaa attatcagg aatgaaact gtgcaatat 
      ctcacgtcc aaatacaaa tttcgaggt catttgggt 
      gaccaggat ttttatact ttgataggg tttgaatat 
      cctgatttg attcaaacc ataaattgt ggtttcaat 
      cgccaattg tgcgtctgg tggcgagag catggctac 
      cgtgatgtt tttgatttt tatttcaag tgtcaacac 
      cccgctgtt attttacat ggaaattgc aacacgaaa 
      attccaaaa taa
    mRNA sequence
    • >TC013781 RA
      aaagcactg tttaaagac caattttcc attaagcag 
      ctttcgcat ttttagttc aatcagtga taaaggcac 
      atttaatta aattaatca aaattcgta atgtggtct 
      tcaaacaaa aagagaggt tatgttgac agttgtttg 
      tgttgtgtt attgtgtaa aggaaaaat agaaatgta 
      acacttaaa aaatgttca attcctgcg acaaacaga 
      gaaatgatt aacgatgta cgcccccaa aaaacctaa 
      actacgatt tttgttaaa acttggaat tttaattgc 
      tgcgttatt agttgttta ttttacgtt ttttcatta 
      caattccga tggttctgc taacgatgc aacattttt 
      gttgccatt caaaaagcc taaagaata caatgtctg 
      gttgatatt tactaaagt ctctcaaag gtcgccgtt 
      aagatataa attccacaa cttgttgga gaatatttt 
      aaacataag ttctgttcc tttaaagtt ccatattat 
      tgttgataa tagctcaaa gcaattagc agtaggtga 
      aatatcaga tgtggtgtc gcattcgaa taaagcaat 
      attttataa attttatga tgttgaagc gtgtgcaag 
      agcgattag tgatattgt cgacgttat gacccctca 
      ttttagttc aaaaccggg tacttacta cagcgacgc 
      acttttcta tatttcttt ggggctgta tcggatagc 
      cccaccttc ccagcgaac tgcccttct actagactg 
      tgatttata ctttaagaa ggatattcg attagtgtt 
      tgaggagtt tgacaagtt taagccaac tgcgctttt 
      tggtttggc ccccgagct gactccagt ctaccgtca 
      cgtcctcca aacctataa agtgaaaaa gaactcaac 
      attcggaga atattatca ttccaaggc gatatcgag 
      caatatgca gcatcctcg tggcttcca gggctataa 
      ttccggtgt tgttttgat taatttaag tgcaataag 
      aaaatcaaa ggaatattc ccaaattat caggaatga 
      aactgtgca atatctcac gtccaaata caaatttcg 
      aggtcattt gggtgacca ggattttta tactttgat 
      agggtttga atatcctga tttgattca aaccataaa 
      ttgtggttt caatcgcca attgtgcgt ctggtggcg 
      agagcatgg ctaccgtga tgtttttga tttttattt 
      caagtgtca acaccccgc tgttatttt acatggaaa 
      ttgcaacac gaaaattcc aaaataaat taaagactg 
      gtcatctat tcgtggaat ctctacacg tccactagt 
      agttttggg aaaatttga gttggcagc attggttat 
      tgccaatca aacaatcat gtggtatga cccggtata 
      ttaggtacg agtgcatta ctgggttgt gaaagtact 
      catttattt tcttgtttg taaaacagt atttttcaa 
      aaataaaca gttttttat ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY