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Gene information
General information
TC013779 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4295766..4340350
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013779
    iBeetle screen iB_05495

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013779 RA
      MYVTQLGPS ILVIFWCFC LPFALSEDS WKCLNYTKT 
      FPKTHVYYL AQHTNIIIQ TSERTLHKL STEILKIYL 
      EEVSGFAKV DILVDDDNF EVDTAVKKL SSRYLNVTD 
      LFPPAMIDL EVWIPPGHD LLNSEDTHL VKDLGDVGP 
      LGRFGWFIP GQLDGPVAR YYGKWDTKF REVHWSFLR 
      DPKIANLLD VSEHMSFIY ANARMNDTS YVCSEKFCK 
      RGIYTPKHC EHTRCALLL TSKPSITKF VVEHINELK 
      LYVRVAWLG KNLRRAIQT LTKFYQNTQ KSFLVLYYT 
      PSDVILRER EFISVVFPQ CDYLDLNGS LGCKYELQR 
      IVKMSWNKV EELGLLRPV RDFKFSEQD YDDLLTLYE 
      RTWNQSNLR QIACEWLKD HESWIIDHG KVEIYIGGI 
      FPLNESTYN GRGIVHGAK LAQDIINAD PSILPNYDL 
      KLLVENGRC RPDSVTKIF IQYVTEYHY NRLLGVLGP 
      ACSDTVEPV THVSKHYRT LVVSYSAEG TSFSDRNKY 
      PYFFRTIGE NKDYQYVYL LLFKKLGWK RVAALTEDG 
      QKYTEYISH MQETLGEND IAFIANTKF PRDRDPYQM 
      SRYLEDLKH KNARIIILD VIDSIARTV MCEAYQMKM 
      TSADGYVWF LPTWLNETW YDTDYFNKF KGESVNCTT 
      AEMVQAATG YFSMAHSFY APNDTLVQG NVTVAQWMN 
      MYGSKVPIS NYAGYAYDA MWTYALALD KLAKEYPAA 
      VSDLHSETT AEKLVQLIQ ETDFMGVSG RIKFRGGPS 
      RFSPINLME WYGNKSHIV GHFYPNISD DKPEILGGV 
      LDLKTESLR WFTPNKHQP DDGTLVPPK CALQSLAKA 
      LNVNCEMAI VILNIILIC VLLLGVFAA VFFMKRRYE 
      KKVKYTEQY MRHFGIDLR LNPSQITDL DKWEIPRNS 
      VVINRKLGE GAFGTVYGG EANFPDKGW VAVAVKTLK 
      VGSSTEEKL DFLSEAEVM KRFDHKNII KLLGVCTKE 
      EPIYTIMEF MLYGDLKTF LLARRHLVN DKTIEESDE 
      ISSKKLTMM ALDVARGLS YLAQLKYVH RDVASRNCL 
      INAQRIVKL ADFGMTRPM FENDYYKFT RKGMLPVRW 
      MSPESLALG VFTPSSDVW SYGVLLYEI ITFGSFPYQ 
      GMTNGEVLE YVKNGHTVT IPQGVKTQL RGLLKSCWH 
      QDPKMRTRA SEIVEFLAN NPRLIVPCL EVPLASVQL 
      EDTNQLEIN LPDQFRKCS TSDIKVSPT TIPNGVAAP 
      PPQYLDTAP TDNCCPREP LLGTSKSNS SLLNFGKYV 
      AIHRDDESK KNEEEGDYL THNATPGNG YVVSNV
    CDS sequence
    • >TC013779 RA
      atgtatgtg acacaactt ggaccgagt attcttgtc 
      atcttttgg tgcttttgc ttgcctttc gcgttatct 
      gaagactca tggaaatgc ctcaattat acaaagaca 
      tttcccaaa acgcacgtc tattacctg gcccaacac 
      accaacata ataatccaa acctccgag cgcactctc 
      cacaaactc agcaccgaa atcctcaaa atctacctg 
      gaggaggtc tccggcttc gccaaagtg gacatcctc 
      gtcgacgac gacaacttc gaggtggac accgccgtc 
      aagaagctc tcatcgcgc tacctcaac gtcaccgac 
      ctgttcccc ccggctatg atcgacctg gaggtgtgg 
      atacccccc ggccacgac ctcctcaac tccgaggac 
      acccacctg gtcaaggac ctgggggac gtgggcccc 
      ttgggccgc ttcggctgg ttcatcccg ggccagctc 
      gacggcccc gtggcccgc tactacggc aagtgggac 
      accaagttc cgggaggtc cactggtcg ttcctccgc 
      gaccccaaa atcgcaaac ctgctggac gtctccgag 
      cacatgtcc ttcatctac gccaacgcg agaatgaac 
      gacacctcc tacgtgtgt tcggagaag ttctgcaaa 
      agggggatc tacactccg aagcattgt gagcacacc 
      aggtgcgcc ctccttctg acctccaag ccctccatc 
      accaagttc gtggtggag cacatcaac gagctcaag 
      ctctacgtc cgggtggcc tggttgggc aagaacctg 
      agacgcgcc atccagact ttgaccaag ttttaccaa 
      aacactcag aagagtttc ctcgtgttg tactacact 
      cccagtgac gtcatcctg cgggagagg gagttcatc 
      agtgtggtt ttcccccag tgcgattac ctggacctg 
      aacggctcg ctcgggtgc aagtacgag ctccagagg 
      attgtgaag atgtcctgg aacaaggtg gaggagttg 
      gggctgttg cgcccagtc cgcgacttc aagttcagc 
      gagcaggac tatgacgac ctcctgacc ctctacgag 
      aggacctgg aaccagtcc aacctgcgc cagattgcc 
      tgcgagtgg ctcaaggac cacgagtcg tggataatc 
      gaccacggc aaggtggag atctacatt gggggcatt 
      ttccccctg aacgagtcc acctacaac ggcaggggc 
      atcgtccac ggggccaag ctggcccaa gatatcatc 
      aacgcggac ccgtcgatc ctgcccaac tacgacctc 
      aagctcctg gtggagaac gggcgctgt cggcccgac 
      agcgtgacg aagattttc atccaatac gtgacggag 
      taccactac aacaggctg ttgggggtg ctggggccg 
      gcgtgttcg gacacggtg gagccggtg acacacgtc 
      tccaagcat taccgcact ctggtggtg tcgtacagt 
      gccgaaggg acgagtttc tccgatcgc aacaaatat 
      ccgtacttt tttcgaacg attggggag aataaggat 
      taccagtac gtttatttg cttctgttc aagaagctg 
      ggctggaag cgggtcgct gcgttgacg gaggacggg 
      caaaagtac accgagtat atctcgcac atgcaggag 
      actttgggg gagaacgat atcgcgttc attgccaat 
      acgaagttt ccgagggat agggacccc tatcaaatg 
      tccagatat ttggaagac ttgaagcac aaaaacgcg 
      cgcatcata atcctcgac gtgatcgac tccattgcc 
      cgaacggtg atgtgcgag gcttatcaa atgaagatg 
      acttcggcc gatggctac gtttggttc cttccaact 
      tggctcaat gaaacctgg tacgatacc gactacttc 
      aacaagttc aaaggtgaa tcggtgaat tgtaccact 
      gctgaaatg gtccaagct gccactggg tacttctca 
      atggctcat tctttctat gccccaaac gatacccta 
      gtccaagga aatgtgaca gtggcacag tggatgaat 
      atgtatgga agcaaagtt ccgatatcg aactacgcg 
      ggatacgct tatgatgct atgtggact tatgctctc 
      gctttggat aagctggcc aaggagtat ccggcggct 
      gtttcggat ttgcattcg gaaacgaca gcagaaaaa 
      ctggtgcag ctaatccag gagacggac ttcatgggc 
      gtctccggg cgcataaaa ttccgcggc ggtccctcc 
      cgtttctcg cccataaac ctgatggaa tggtacggg 
      aacaaatcg cacatcgtg ggccacttc tacccgaac 
      atctccgac gacaaaccg gagatcctc ggcggcgtt 
      ctcgacctc aaaaccgag tctttgcgc tggttcact 
      cccaacaag caccaacca gacgacggc accctggtc 
      cccccgaaa tgcgccctc cagagcctc gccaaggcc 
      ctcaacgtc aactgcgag atggccatc gtcatcctc 
      aacatcatc ctcatctgc gtcctcctc ctgggcgtc 
      ttcgccgca gtgttcttc atgaagcga cgatacgag 
      aagaaggtc aagtacacg gagcagtac atgcgccac 
      ttcggcatc gacctgcgc ctgaacccc tcgcaaatc 
      accgacctg gacaagtgg gagattccc cggaactcg 
      gtcgtgatc aaccgcaag ctgggggag ggcgccttc 
      gggacggtg tacggcggc gaggccaac ttcccggac 
      aagggctgg gtggccgtg gccgtgaag acgctcaag 
      gtcggctcc agcacggag gagaagctg gacttcttg 
      tcggaggcg gaggtgatg aagcggttc gaccacaag 
      aacattatc aaactgctg ggcgtttgc accaaagag 
      gagccgatt tacaccatc atggagttc atgctttac 
      ggcgatttg aagacgttt ctgctcgcc aggaggcac 
      ctagtcaat gataagacc atagaggag tcggacgaa 
      atctccagt aagaagctg acgatgatg gcgttggat 
      gtggcgaga gggctgagt tatttggcg cagttgaag 
      tacgtccat cgagatgtt gcgtcgagg aattgcctg 
      atcaatgct caaaggatt gtgaagttg gcggatttc 
      gggatgacg aggcccatg ttcgagaat gattattac 
      aagtttacg aggaagggg atgttgccg gtgcggtgg 
      atgtcgccg gagagtttg gccttgggg gtgtttacg 
      ccctcgagt gacgtctgg agttatggg gtgctgttg 
      tatgagatt atcaccttt gggagcttc ccctaccag 
      gggatgacc aatggagag gtgttggag tatgtcaag 
      aatggacac actgtgacg attccgcaa ggagttaag 
      acgcaactg aggggcctg ctgaagtcc tgctggcac 
      caggacccg aaaatgcgc acccgcgcg tcggaaatc 
      gtcgaattc ctcgccaac aacccgcgc ctcatcgtc 
      ccctgcctg gaagtgccc ctggcctcg gtccagctc 
      gaggacacc aaccagctc gaaatcaac ctcccggac 
      cagttccgc aaatgctcc accagcgac attaaagtc 
      tccccgacg acgatcccc aacggcgtc gccgcccct 
      ccgccccag tacctcgac accgccccc acggacaac 
      tgctgcccc cgcgagccc ctgctgggc acctccaag 
      tccaactcg agcctcctc aacttcggc aagtacgtc 
      gccatccat cgcgacgac gagagcaag aaaaacgag 
      gaagagggc gattatcta acgcataat gcaacacct 
      gggaatggc tacgttgtc tccaatgtc taa
    mRNA sequence
    • >TC013779 RA
      ctaaaatat taattaaaa ttactgtgt aaacacgga 
      cgaattttt ctatttcag gttggatgc aagtgagga 
      gtgcagcta tcttccgat gaggcaaag atcgttcgt 
      ttgattgca ccgttagcc aagtgaagt aaaatgtat 
      gtgacacaa cttggaccg agtattctt gtcatcttt 
      tggtgcttt tgcttgcct ttcgcgtta tctgaagac 
      tcatggaaa tgcctcaat tatacaaag acatttccc 
      aaaacgcac gtctattac ctggcccaa cacaccaac 
      ataataatc caaacctcc gagcgcact ctccacaaa 
      ctcagcacc gaaatcctc aaaatctac ctggaggag 
      gtctccggc ttcgccaaa gtggacatc ctcgtcgac 
      gacgacaac ttcgaggtg gacaccgcc gtcaagaag 
      ctctcatcg cgctacctc aacgtcacc gacctgttc 
      cccccggct atgatcgac ctggaggtg tggataccc 
      cccggccac gacctcctc aactccgag gacacccac 
      ctggtcaag gacctgggg gacgtgggc cccttgggc 
      cgcttcggc tggttcatc ccgggccag ctcgacggc 
      cccgtggcc cgctactac ggcaagtgg gacaccaag 
      ttccgggag gtccactgg tcgttcctc cgcgacccc 
      aaaatcgca aacctgctg gacgtctcc gagcacatg 
      tccttcatc tacgccaac gcgagaatg aacgacacc 
      tcctacgtg tgttcggag aagttctgc aaaaggggg 
      atctacact ccgaagcat tgtgagcac accaggtgc 
      gccctcctt ctgacctcc aagccctcc atcaccaag 
      ttcgtggtg gagcacatc aacgagctc aagctctac 
      gtccgggtg gcctggttg ggcaagaac ctgagacgc 
      gccatccag actttgacc aagttttac caaaacact 
      cagaagagt ttcctcgtg ttgtactac actcccagt 
      gacgtcatc ctgcgggag agggagttc atcagtgtg 
      gttttcccc cagtgcgat tacctggac ctgaacggc 
      tcgctcggg tgcaagtac gagctccag aggattgtg 
      aagatgtcc tggaacaag gtggaggag ttggggctg 
      ttgcgccca gtccgcgac ttcaagttc agcgagcag 
      gactatgac gacctcctg accctctac gagaggacc 
      tggaaccag tccaacctg cgccagatt gcctgcgag 
      tggctcaag gaccacgag tcgtggata atcgaccac 
      ggcaaggtg gagatctac attgggggc attttcccc 
      ctgaacgag tccacctac aacggcagg ggcatcgtc 
      cacggggcc aagctggcc caagatatc atcaacgcg 
      gacccgtcg atcctgccc aactacgac ctcaagctc 
      ctggtggag aacgggcgc tgtcggccc gacagcgtg 
      acgaagatt ttcatccaa tacgtgacg gagtaccac 
      tacaacagg ctgttgggg gtgctgggg ccggcgtgt 
      tcggacacg gtggagccg gtgacacac gtctccaag 
      cattaccgc actctggtg gtgtcgtac agtgccgaa 
      gggacgagt ttctccgat cgcaacaaa tatccgtac 
      ttttttcga acgattggg gagaataag gattaccag 
      tacgtttat ttgcttctg ttcaagaag ctgggctgg 
      aagcgggtc gctgcgttg acggaggac gggcaaaag 
      tacaccgag tatatctcg cacatgcag gagactttg 
      ggggagaac gatatcgcg ttcattgcc aatacgaag 
      tttccgagg gatagggac ccctatcaa atgtccaga 
      tatttggaa gacttgaag cacaaaaac gcgcgcatc 
      ataatcctc gacgtgatc gactccatt gcccgaacg 
      gtgatgtgc gaggcttat caaatgaag atgacttcg 
      gccgatggc tacgtttgg ttccttcca acttggctc 
      aatgaaacc tggtacgat accgactac ttcaacaag 
      ttcaaaggt gaatcggtg aattgtacc actgctgaa 
      atggtccaa gctgccact gggtacttc tcaatggct 
      cattctttc tatgcccca aacgatacc ctagtccaa 
      ggaaatgtg acagtggca cagtggatg aatatgtat 
      ggaagcaaa gttccgata tcgaactac gcgggatac 
      gcttatgat gctatgtgg acttatgct ctcgctttg 
      gataagctg gccaaggag tatccggcg gctgtttcg 
      gatttgcat tcggaaacg acagcagaa aaactggtg 
      cagctaatc caggagacg gacttcatg ggcgtctcc 
      gggcgcata aaattccgc ggcggtccc tcccgtttc 
      tcgcccata aacctgatg gaatggtac gggaacaaa 
      tcgcacatc gtgggccac ttctacccg aacatctcc 
      gacgacaaa ccggagatc ctcggcggc gttctcgac 
      ctcaaaacc gagtctttg cgctggttc actcccaac 
      aagcaccaa ccagacgac ggcaccctg gtccccccg 
      aaatgcgcc ctccagagc ctcgccaag gccctcaac 
      gtcaactgc gagatggcc atcgtcatc ctcaacatc 
      atcctcatc tgcgtcctc ctcctgggc gtcttcgcc 
      gcagtgttc ttcatgaag cgacgatac gagaagaag 
      gtcaagtac acggagcag tacatgcgc cacttcggc 
      atcgacctg cgcctgaac ccctcgcaa atcaccgac 
      ctggacaag tgggagatt ccccggaac tcggtcgtg 
      atcaaccgc aagctgggg gagggcgcc ttcgggacg 
      gtgtacggc ggcgaggcc aacttcccg gacaagggc 
      tgggtggcc gtggccgtg aagacgctc aaggtcggc 
      tccagcacg gaggagaag ctggacttc ttgtcggag 
      gcggaggtg atgaagcgg ttcgaccac aagaacatt 
      atcaaactg ctgggcgtt tgcaccaaa gaggagccg 
      atttacacc atcatggag ttcatgctt tacggcgat 
      ttgaagacg tttctgctc gccaggagg cacctagtc 
      aatgataag accatagag gagtcggac gaaatctcc 
      agtaagaag ctgacgatg atggcgttg gatgtggcg 
      agagggctg agttatttg gcgcagttg aagtacgtc 
      catcgagat gttgcgtcg aggaattgc ctgatcaat 
      gctcaaagg attgtgaag ttggcggat ttcgggatg 
      acgaggccc atgttcgag aatgattat tacaagttt 
      acgaggaag gggatgttg ccggtgcgg tggatgtcg 
      ccggagagt ttggccttg ggggtgttt acgccctcg 
      agtgacgtc tggagttat ggggtgctg ttgtatgag 
      attatcacc tttgggagc ttcccctac caggggatg 
      accaatgga gaggtgttg gagtatgtc aagaatgga 
      cacactgtg acgattccg caaggagtt aagacgcaa 
      ctgaggggc ctgctgaag tcctgctgg caccaggac 
      ccgaaaatg cgcacccgc gcgtcggaa atcgtcgaa 
      ttcctcgcc aacaacccg cgcctcatc gtcccctgc 
      ctggaagtg cccctggcc tcggtccag ctcgaggac 
      accaaccag ctcgaaatc aacctcccg gaccagttc 
      cgcaaatgc tccaccagc gacattaaa gtctccccg 
      acgacgatc cccaacggc gtcgccgcc cctccgccc 
      cagtacctc gacaccgcc cccacggac aactgctgc 
      ccccgcgag cccctgctg ggcacctcc aagtccaac 
      tcgagcctc ctcaacttc ggcaagtac gtcgccatc 
      catcgcgac gacgagagc aagaaaaac gaggaagag 
      ggcgattat ctaacgcat aatgcaaca cctgggaat 
      ggctacgtt gtctccaat gtctaatct gactttcaa 
      tacgtaatt tttcgttga gagcctttt ttgcatttc 
      cacgataca gttgttggt tctagtcat ttgatatgt 
      cgcttaatc gaaatcatt cgtccaata gtattaacg 
      tgtattaat tttgttcta tgagcggaa ggcctgtgg 
      caagtccat gactattct tcgagttcc ttagcacat 
      gaaataaga ctttaaggg gacactccc gaaacaggc 
      ggtttttat ttttaaaac gatcatatt ttattagtt 
      aagaatatt ttaaacttg tgtcgcact gtatattgg 
      ataccaaac gattatatc aaatattta tttttaagg 
      agtgagcgc ttgatgagt gggtgaatg acgcgtgtt 
      taaaacaat tacattttg tacctgacg ttacttttt 
      taagtaata tttaatatc taagattat taataaata 
      ctttcaata ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY