Status
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Gene information
General information
TC033050
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013772,TC013771
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4471825..4481058
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033050
    iBeetle screen iB_05494

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen musculature posterior decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature posterior decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva posterior decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033050 RA
      MVTIFVICN TLLIIGGII LNLIPLSDD LHVYFALRF 
      IHEYFPNHK TCLIILLKA SIFPVIPHM LVVHAYQIL 
      YYTQHSNFQ IQLFNKVIA EVDFWETPL RETELFYSK 
      PYQKGIEKK LKFCIQRLQ VLINAYIVK TKEIGTLIA 
      LFAICGVLM GIGFSLYLF SGKFTPEYY LRLTFMTLV 
      AVTTFSSII WGGQSTETI ITEMITALC QVRWYNFSQ 
      TNKKLYLIL LTNMMKDRK IKFTENYSI NYQLGLAIV 
      RGIYSIIYM LTEFYSVSD RQFAMFIFA ALFFLIIFY 
      LYHQNVKLN RILCTFPEP PKKFLLGHV LDITSTTDV 
      LNVFSKYIN NYGPTIKLR AATLFAGLG TTDYKLCEL 
      LLSNNRILS KSLNYQYAR NWLGNGLLI SDGDYWRRH 
      RKILTPAFH FEILKQFVE TFESVGKVF VEKLDISQG 
      PSVDLPPLV TLCTLDVIC ETAMGTQIN AQKGQNAKY 
      VQSVREMCR ILIDRGLSP LKILNNTYW MTKDYYIEK 
      KSLKVLHSF TSSVIEKRK AERNERNCT KLAFLDLLL 
      KFSNEGELL TDQELREEV DTFMFEGHD TTASSISFV 
      LFCLANHPE VQEKVLREQ NELFGDDKD PSVTYHELQ 
      KMKYLEQVI KETLRLYPA VPIIGRCTS EDITFGEHF 
      IPKDTNIAI YIYGIHRNP EHFPEPETF NPDRFKNSN 
      SLPPYAYIP FSAGPRNCI GQKFAMLEI KSIVSRVVR 
      CFELRPAEP YHSLVLSAE TVLKSANGI KIGIRKRI
    • >TC033050 RB
      MVTIFVICN TLLIIGGII LNLIPLSDD LHVYFALRF 
      IHEYFPNHK TCLIILLKA SIFPVIPHM LVVHAYQIL 
      YYTQHSNFQ IQLFNKVIA EVDFWETPL RETELFYSK 
      PYQKGIEKK LKFCIQRLQ VLINAYIVK TKEIGTLIA 
      LFAICGVLM GIGFSLYLF SGKFTPEYY LRLTFMTLV 
      AVTTFSSII WGGQSTETI ITEMITALC QVRWYNFSQ 
      TNKKLYLIL LTNMMKDRK IKFTENYSI NYQLGLAVL 
      SDRQFAMFI FAALFFLII FYLYHQNVK LNRILCTFP 
      EPPKKFLLG HVLDITSTT DVLNVFSKY INNYGPTIK 
      LRAATLFAG LGTTDYKLC ELLLSNNRI LSKSLNYQY 
      ARNWLGNGL LISDGDYWR RHRKILTPA FHFEILKQF 
      VETFESVGK VFVEKLDIS QGPSVDLPP LVTLCTLDV 
      ICETAMGTQ INAQKGQNA KYVQSVREM CRILIDRGL 
      SPLKILNNT YWMTKDYYI EKKSLKVLH SFTSSVIEK 
      RKAERNERN CTKLAFLDL LLKFSNEGE LLTDQELRE 
      EVDTFMFEG HDTTASSIS FVLFCLANH PEVQEKVLR 
      EQNELFGDD KDPSVTYHE LQKMKYLEQ VIKETLRLY 
      PAVPIIGRC TSEDITFGE HFIPKDTNI AIYIYGIHR 
      NPEHFPEPE TFNPDRFKN SNSLPPYAY IPFSAGPRN 
      CIGQKFAML EIKSIVSRV VRCFELRPA EPYHSLVLS 
      AETVLKSAN GIKIGIRKR I
    CDS sequence
    • >TC033050 RA
      atggtgaca atttttgtt atttgcaat actctactc 
      attattggt ggtattata ctcaaccta atacctctg 
      tcggacgat cttcatgtt tatttcgct ttgcgtttc 
      atccacgaa tattttccc aatcataaa acatgtctt 
      ataattctt ctgaaagct tcaatcttt cctgtcatc 
      cctcacatg ctagtagtg cacgcttac caaattctc 
      tactatact cagcactca aatttccaa attcagtta 
      tttaacaaa gttatcgca gaggttgat ttttgggaa 
      acgccttta cgtgaaact gagctgttt tacagtaaa 
      ccctatcaa aaaggaatc gaaaagaaa ctaaagttt 
      tgcattcaa agacttcag gtgttaatc aatgcctac 
      attgttaaa actaaagaa attggtacg ttaattgca 
      ctgtttgca atttgtggc gtccttatg ggaattgga 
      ttttcttta tatttattt tctggaaaa tttactcct 
      gagtattat ttacgactg actttcatg acgcttgtt 
      gctgttacg actttttcg tccattatc tggggaggt 
      caatcgact gaaactatc ataactgaa atgattacg 
      gcattatgt caagttcgg tggtacaat tttagtcaa 
      accaataaa aaactctat ttaattctt ttaacaaac 
      atgatgaaa gatcggaaa atcaaattt acggagaat 
      tattccatc aactaccag ctaggactg gccatcgtc 
      agaggaatt tactcgatc atttacatg ttgactgaa 
      ttttattca gtttctgat cgacagttc gcaatgttt 
      atatttgca gcgcttttt ttcctaata attttctat 
      ctataccac caaaacgtg aaactgaat cgtatcttg 
      tgcactttt ccggaacca ccgaaaaaa tttctgttg 
      ggacatgtg ctcgatata acgtcaaca acggacgtt 
      ctcaacgtt ttttccaag tatataaac aattatgga 
      cccactatt aaattgcga gcggctacg cttttcgca 
      ggacttgga acaacggac tataaactg tgcgaactt 
      ttactgagt aataaccga attttgtcg aaatctcta 
      aattaccaa tacgctcga aactggctc ggaaacggc 
      ctcctgatc agtgatggt gattattgg aggcgacat 
      cgcaaaatt ctcacgccg gctttccac ttcgaaatc 
      ctcaaacaa tttgtcgag acttttgag tcggtgggt 
      aaagtgttc gttgaaaaa ttggacatt tctcaaggg 
      cccagtgtt gacctgcca cctctggtc actttgtgc 
      actttggac gtgatttgt gaaactgca atgggcacg 
      caaatcaac gctcagaaa ggccaaaat gcgaaatac 
      gtccaaagt gtgagggaa atgtgccga attttgata 
      gatcgagga ctatcgcct ttgaaaatt ctcaacaac 
      acgtattgg atgactaaa gattattac attgagaaa 
      aagtcgctc aaggttttg cacagcttc acttcaagt 
      gtgatcgag aaacgcaaa gcggagcga aacgagaga 
      aattgcacc aaattggct tttttggac ttgttattg 
      aaattttcg aacgaaggc gagctactg actgaccag 
      gaattgcgc gaggaagtt gatactttc atgtttgag 
      ggtcatgac accacggca tcttcaatt tctttcgtc 
      ttgttttgt ttagcaaat catccggaa gtgcaagag 
      aaagtttta agagaacaa aacgaattg ttcggtgat 
      gacaaagac ccaagtgtg acttatcac gagttgcaa 
      aaaatgaag tatttggag caagtgatt aaagaaact 
      ttgagattg tatcctgct gttccaata attggccgg 
      tgcacttcg gaggacata acttttggg gaacatttc 
      attccaaag gacacgaac atagcaatt tacatttac 
      ggcattcac agaaatccg gaacatttt ccggaaccg 
      gaaacgttc aaccctgac agattcaaa aattcgaac 
      agtttgcct ccgtatgct tacattccg ttcagtgcc 
      gggcccagg aattgcatc ggccaaaaa tttgcaatg 
      ttggaaatt aagagtatt gtttctcga gttgtgagg 
      tgttttgag ttaagaccg gctgagcct tatcacagt 
      ttggtgttg tcggctgaa acggtcctg aagtcggca 
      aatgggatc aaaattggg atcaggaaa cgaatctag
    • >TC033050 RB
      atggtgaca atttttgtt atttgcaat actctactc 
      attattggt ggtattata ctcaaccta atacctctg 
      tcggacgat cttcatgtt tatttcgct ttgcgtttc 
      atccacgaa tattttccc aatcataaa acatgtctt 
      ataattctt ctgaaagct tcaatcttt cctgtcatc 
      cctcacatg ctagtagtg cacgcttac caaattctc 
      tactatact cagcactca aatttccaa attcagtta 
      tttaacaaa gttatcgca gaggttgat ttttgggaa 
      acgccttta cgtgaaact gagctgttt tacagtaaa 
      ccctatcaa aaaggaatc gaaaagaaa ctaaagttt 
      tgcattcaa agacttcag gtgttaatc aatgcctac 
      attgttaaa actaaagaa attggtacg ttaattgca 
      ctgtttgca atttgtggc gtccttatg ggaattgga 
      ttttcttta tatttattt tctggaaaa tttactcct 
      gagtattat ttacgactg actttcatg acgcttgtt 
      gctgttacg actttttcg tccattatc tggggaggt 
      caatcgact gaaactatc ataactgaa atgattacg 
      gcattatgt caagttcgg tggtacaat tttagtcaa 
      accaataaa aaactctat ttaattctt ttaacaaac 
      atgatgaaa gatcggaaa atcaaattt acggagaat 
      tattccatc aactaccag ctaggactg gccgtactt 
      tctgatcga cagttcgca atgtttata tttgcagcg 
      ctttttttc ctaataatt ttctatcta taccaccaa 
      aacgtgaaa ctgaatcgt atcttgtgc acttttccg 
      gaaccaccg aaaaaattt ctgttggga catgtgctc 
      gatataacg tcaacaacg gacgttctc aacgttttt 
      tccaagtat ataaacaat tatggaccc actattaaa 
      ttgcgagcg gctacgctt ttcgcagga cttggaaca 
      acggactat aaactgtgc gaactttta ctgagtaat 
      aaccgaatt ttgtcgaaa tctctaaat taccaatac 
      gctcgaaac tggctcgga aacggcctc ctgatcagt 
      gatggtgat tattggagg cgacatcgc aaaattctc 
      acgccggct ttccacttc gaaatcctc aaacaattt 
      gtcgagact tttgagtcg gtgggtaaa gtgttcgtt 
      gaaaaattg gacatttct caagggccc agtgttgac 
      ctgccacct ctggtcact ttgtgcact ttggacgtg 
      atttgtgaa actgcaatg ggcacgcaa atcaacgct 
      cagaaaggc caaaatgcg aaatacgtc caaagtgtg 
      agggaaatg tgccgaatt ttgatagat cgaggacta 
      tcgcctttg aaaattctc aacaacacg tattggatg 
      actaaagat tattacatt gagaaaaag tcgctcaag 
      gttttgcac agcttcact tcaagtgtg atcgagaaa 
      cgcaaagcg gagcgaaac gagagaaat tgcaccaaa 
      ttggctttt ttggacttg ttattgaaa ttttcgaac 
      gaaggcgag ctactgact gaccaggaa ttgcgcgag 
      gaagttgat actttcatg tttgagggt catgacacc 
      acggcatct tcaatttct ttcgtcttg ttttgttta 
      gcaaatcat ccggaagtg caagagaaa gttttaaga 
      gaacaaaac gaattgttc ggtgatgac aaagaccca 
      agtgtgact tatcacgag ttgcaaaaa atgaagtat 
      ttggagcaa gtgattaaa gaaactttg agattgtat 
      cctgctgtt ccaataatt ggccggtgc acttcggag 
      gacataact tttggggaa catttcatt ccaaaggac 
      acgaacata gcaatttac atttacggc attcacaga 
      aatccggaa cattttccg gaaccggaa acgttcaac 
      cctgacaga ttcaaaaat tcgaacagt ttgcctccg 
      tatgcttac attccgttc agtgccggg cccaggaat 
      tgcatcggc caaaaattt gcaatgttg gaaattaag 
      agtattgtt tctcgagtt gtgaggtgt tttgagtta 
      agaccggct gagccttat cacagtttg gtgttgtcg 
      gctgaaacg gtcctgaag tcggcaaat gggatcaaa 
      attgggatc aggaaacga atctag
    mRNA sequence
    • >TC033050 RA
      tggtgagaa aatccactc cgaaattag gtttaaagc 
      caggatggt gacaatttt tgttatttg caatactct 
      actcattat tggtggtat tatactcaa cctaatacc 
      tctgtcgga cgatcttca tgtttattt cgctttgcg 
      tttcatcca cgaatattt tcccaatca taaaacatg 
      tcttataat tcttctgaa agcttcaat ctttcctgt 
      catccctca catgctagt agtgcacgc ttaccaaat 
      tctctacta tactcagca ctcaaattt ccaaattca 
      gttatttaa caaagttat cgcagaggt tgatttttg 
      ggaaacgcc tttacgtga aactgagct gttttacag 
      taaacccta tcaaaaagg aatcgaaaa gaaactaaa 
      gttttgcat tcaaagact tcaggtgtt aatcaatgc 
      ctacattgt taaaactaa agaaattgg tacgttaat 
      tgcactgtt tgcaatttg tggcgtcct tatgggaat 
      tggattttc tttatattt attttctgg aaaatttac 
      tcctgagta ttatttacg actgacttt catgacgct 
      tgttgctgt tacgacttt ttcgtccat tatctgggg 
      aggtcaatc gactgaaac tatcataac tgaaatgat 
      tacggcatt atgtcaagt tcggtggta caattttag 
      tcaaaccaa taaaaaact ctatttaat tcttttaac 
      aaacatgat gaaagatcg gaaaatcaa atttacgga 
      gaattattc catcaacta ccagctagg actggccat 
      cgtcagagg aatttactc gatcattta catgttgac 
      tgaatttta ttcagtttc tgatcgaca gttcgcaat 
      gtttatatt tgcagcgct ttttttcct aataatttt 
      ctatctata ccaccaaaa cgtgaaact gaatcgtat 
      cttgtgcac ttttccgga accaccgaa aaaatttct 
      gttgggaca tgtgctcga tataacgtc aacaacgga 
      cgttctcaa cgttttttc caagtatat aaacaatta 
      tggacccac tattaaatt gcgagcggc tacgctttt 
      cgcaggact tggaacaac ggactataa actgtgcga 
      acttttact gagtaataa ccgaatttt gtcgaaatc 
      tctaaatta ccaatacgc tcgaaactg gctcggaaa 
      cggcctcct gatcagtga tggtgatta ttggaggcg 
      acatcgcaa aattctcac gccggcttt ccacttcga 
      aatcctcaa acaatttgt cgagacttt tgagtcggt 
      gggtaaagt gttcgttga aaaattgga catttctca 
      agggcccag tgttgacct gccacctct ggtcacttt 
      gtgcacttt ggacgtgat ttgtgaaac tgcaatggg 
      cacgcaaat caacgctca gaaaggcca aaatgcgaa 
      atacgtcca aagtgtgag ggaaatgtg ccgaatttt 
      gatagatcg aggactatc gcctttgaa aattctcaa 
      caacacgta ttggatgac taaagatta ttacattga 
      gaaaaagtc gctcaaggt tttgcacag cttcacttc 
      aagtgtgat cgagaaacg caaagcgga gcgaaacga 
      gagaaattg caccaaatt ggctttttt ggacttgtt 
      attgaaatt ttcgaacga aggcgagct actgactga 
      ccaggaatt gcgcgagga agttgatac tttcatgtt 
      tgagggtca tgacaccac ggcatcttc aatttcttt 
      cgtcttgtt ttgtttagc aaatcatcc ggaagtgca 
      agagaaagt tttaagaga acaaaacga attgttcgg 
      tgatgacaa agacccaag tgtgactta tcacgagtt 
      gcaaaaaat gaagtattt ggagcaagt gattaaaga 
      aactttgag attgtatcc tgctgttcc aataattgg 
      ccggtgcac ttcggagga cataacttt tggggaaca 
      tttcattcc aaaggacac gaacatagc aatttacat 
      ttacggcat tcacagaaa tccggaaca ttttccgga 
      accggaaac gttcaaccc tgacagatt caaaaattc 
      gaacagttt gcctccgta tgcttacat tccgttcag 
      tgccgggcc caggaattg catcggcca aaaatttgc 
      aatgttgga aattaagag tattgtttc tcgagttgt 
      gaggtgttt tgagttaag accggctga gccttatca 
      cagtttggt gttgtcggc tgaaacggt cctgaagtc 
      ggcaaatgg gatcaaaat tgggatcag gaaacgaat 
      ctagcaaga actgaatat tttgtaaaa ctgtaaata 
      aattaaata aataaaa
    • >TC033050 RB
      tggtgagaa aatccactc cgaaattag gtttaaagc 
      caggatggt gacaatttt tgttatttg caatactct 
      actcattat tggtggtat tatactcaa cctaatacc 
      tctgtcgga cgatcttca tgtttattt cgctttgcg 
      tttcatcca cgaatattt tcccaatca taaaacatg 
      tcttataat tcttctgaa agcttcaat ctttcctgt 
      catccctca catgctagt agtgcacgc ttaccaaat 
      tctctacta tactcagca ctcaaattt ccaaattca 
      gttatttaa caaagttat cgcagaggt tgatttttg 
      ggaaacgcc tttacgtga aactgagct gttttacag 
      taaacccta tcaaaaagg aatcgaaaa gaaactaaa 
      gttttgcat tcaaagact tcaggtgtt aatcaatgc 
      ctacattgt taaaactaa agaaattgg tacgttaat 
      tgcactgtt tgcaatttg tggcgtcct tatgggaat 
      tggattttc tttatattt attttctgg aaaatttac 
      tcctgagta ttatttacg actgacttt catgacgct 
      tgttgctgt tacgacttt ttcgtccat tatctgggg 
      aggtcaatc gactgaaac tatcataac tgaaatgat 
      tacggcatt atgtcaagt tcggtggta caattttag 
      tcaaaccaa taaaaaact ctatttaat tcttttaac 
      aaacatgat gaaagatcg gaaaatcaa atttacgga 
      gaattattc catcaacta ccagctagg actggccgt 
      actttctga tcgacagtt cgcaatgtt tatatttgc 
      agcgctttt tttcctaat aattttcta tctatacca 
      ccaaaacgt gaaactgaa tcgtatctt gtgcacttt 
      tccggaacc accgaaaaa atttctgtt gggacatgt 
      gctcgatat aacgtcaac aacggacgt tctcaacgt 
      tttttccaa gtatataaa caattatgg acccactat 
      taaattgcg agcggctac gcttttcgc aggacttgg 
      aacaacgga ctataaact gtgcgaact tttactgag 
      taataaccg aattttgtc gaaatctct aaattacca 
      atacgctcg aaactggct cggaaacgg cctcctgat 
      cagtgatgg tgattattg gaggcgaca tcgcaaaat 
      tctcacgcc ggctttcca cttcgaaat cctcaaaca 
      atttgtcga gacttttga gtcggtggg taaagtgtt 
      cgttgaaaa attggacat ttctcaagg gcccagtgt 
      tgacctgcc acctctggt cactttgtg cactttgga 
      cgtgatttg tgaaactgc aatgggcac gcaaatcaa 
      cgctcagaa aggccaaaa tgcgaaata cgtccaaag 
      tgtgaggga aatgtgccg aattttgat agatcgagg 
      actatcgcc tttgaaaat tctcaacaa cacgtattg 
      gatgactaa agattatta cattgagaa aaagtcgct 
      caaggtttt gcacagctt cacttcaag tgtgatcga 
      gaaacgcaa agcggagcg aaacgagag aaattgcac 
      caaattggc ttttttgga cttgttatt gaaattttc 
      gaacgaagg cgagctact gactgacca ggaattgcg 
      cgaggaagt tgatacttt catgtttga gggtcatga 
      caccacggc atcttcaat ttctttcgt cttgttttg 
      tttagcaaa tcatccgga agtgcaaga gaaagtttt 
      aagagaaca aaacgaatt gttcggtga tgacaaaga 
      cccaagtgt gacttatca cgagttgca aaaaatgaa 
      gtatttgga gcaagtgat taaagaaac tttgagatt 
      gtatcctgc tgttccaat aattggccg gtgcacttc 
      ggaggacat aacttttgg ggaacattt cattccaaa 
      ggacacgaa catagcaat ttacattta cggcattca 
      cagaaatcc ggaacattt tccggaacc ggaaacgtt 
      caaccctga cagattcaa aaattcgaa cagtttgcc 
      tccgtatgc ttacattcc gttcagtgc cgggcccag 
      gaattgcat cggccaaaa atttgcaat gttggaaat 
      taagagtat tgtttctcg agttgtgag gtgttttga 
      gttaagacc ggctgagcc ttatcacag tttggtgtt 
      gtcggctga aacggtcct gaagtcggc aaatgggat 
      caaaattgg gatcaggaa acgaatcta gcaagaact 
      gaatatttt gtaaaactg taaataaat taaataaat 
      aaaa
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