| General information | |||
| TC013762 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 4658797..4673495 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013762 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05492 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
ovary without detectable defects - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
- >TC013762 RA
MGKKKGKGK AQEGSGEPS QPAPGASAP STSQPPPQQ QQQRQRGGP QGEGPGPRQ QHPQQRPQQ QRQQQQPQP HPQQQWQQQ PQPHPQQQW QQQQPQPHP QQRQQQQPQ QQWQQQQPQ PHPQQWHQQ PQPHPQQQW QQQQPQPHP QQRQQQQPQ QQWQQQQPQ PHPQQWHQQ QPQQQRGPR QQQGRPRQQ FPPGIQQQR SPSPQQQYG APPPHWGPP GIVPQQPMG QQVQGPPPQ QPPRGPPPG MGAPQQQQP QRGPLPQQQ QQQQQRRPQ QQQPPVKSP VPHPEPSSP PRQEPAPPL SGGGDCLSG ALVVTPGTK GRRIQIESN HLSLNLGKL TEAYHYDVA ITPDTPKCL LRDVMNLFG RKHYPQNHP AFDGRKNLY SPKKLPFPN DTKSDTIEV EGENRKKEF KVEVKLART VDLTPLHDI MRTTQSPQD ALQCLDIVL RNAPSNACI IAGRCFFTP PRDGQIIPL GDGMELYYG FYQSAIRGW KALLNVDVA HKAFPKASN VLDIVCEIG SDFRTTMTR ANLSQPLRE FVQRDFEKF IKQLKVKYE IPNQSSSKR IHRVNGLGE PPSQAKFKL DDGRMTTVE RYYQEVKRC KLQYPHLPT LWVGSRERE SKILLPLEF CTVVGGQAI NRKMNENQT SAMIRKAAT STDVRKDKI MQTLRTANY NNDPCIREF GFSVSNNFE KLDARVLNP PSLLYADNA QIKPSKGVW RADRNRFLV GATINKWTI ASGTRYPSR DADKLADMI FRMASSNGM QITSKATPS THIGGRQGL RDFIDYFKG KQDYDLIIV VVPNSGPQY SFVKQAAEL NVGCLTQCI KERTIGRLN PQTVGNILL KINSKMNGT NHRLSPNSR PLIMKRPCM IMGADVTHP SPDARDIPS VAAVTASHD PNAFQYNIC WRLQPPKVE IIEDLCNIT VEQLKFFYQ KTGFKPESI VFFRDGVSE GQFKQVQRA EIAAIQKAC KMLQKDDYE PKITFLVVQ KRHHTRLFP TNPRDSEDK NNNVPAGTC VDTHITNPR MQDFYLVSH ASIQGVAKP TKYCTLWDD NNMNNDDIE ELTYHLCHM FTRCNRSVS YPAPTYYAH LAAARAKVY IENDKLDMS QLKRHQEKC QIQEKIVKG KPMFFV
- >TC013762 RA
atgggaaag aaaaaagga aagggaaaa gcccaggag ggttctggc gagccatct cagcctgca cctggagct tccgcccca tcaacatct caaccaccc ccacagcaa caacaacag aggcaacgt ggtggccct caaggggaa ggcccaggg ccacgtcag caacatccc caacagagg ccacaacaa cagaggcaa caacaacag cctcagcca catcctcaa caacaatgg caacaacag cctcagcca catcctcaa caacaatgg caacaacaa cagcctcag ccacatccc caacagagg caacagcag cagccccaa caacagtgg caacaacaa cagcctcaa ccacatccc caacagtgg catcaacag cctcagcca catcctcaa caacaatgg caacaacaa cagcctcag ccacatccc caacagagg caacagcag cagccccaa caacagtgg caacaacaa cagcctcaa ccacatccc caacagtgg catcaacaa cagcctcaa cagcaaaga ggcccacgt cagcagcag gggcgtcct agacagcag tttccgcca ggtattcaa cagcagagg agccccagc ccacaacag caatatggg gctcctccg ccacactgg gggcctccc ggtattgtt ccacaacaa cctatggga caacaggtt caaggtcca cctccgcaa cagccccca cgtggacct ccacctgga atgggcgcc ccccaacag cagcaacct caacgaggt ccccttcca cagcagcaa caacaacaa caacaacgt cgtccccaa caacagcaa ccgcccgtt aaatctccc gttcctcat ccagaacct tcgtctcct ccaagacaa gaaccagca cctcctctt agtggcgga ggcgactgt ctttcggga gctcttgtt gtcacacca ggcaccaaa ggcagacgt atacaaatc gaaagcaac catttgtcc ctaaatctg ggcaaactc accgaggcg taccattac gacgttgcg ataacacct gacactccc aaatgttta cttcgcgat gtaatgaat ctcttcggt cgcaaacac tatccgcaa aaccatcca gctttcgac ggcagaaaa aacctctac tctccgaag aaattaccg tttcctaat gacaccaaa agcgatacg attgaagtc gaaggagag aatcgcaaa aaggagttc aaagtcgag gttaaattg gcgagaacg gtcgatttg acgcctctt catgacatc atgaggacg acgcaatcg ccgcaggac gccctccag tgcctggat atcgtcctc cggaatgca ccctccaac gcttgtatc atcgccgga aggtgcttt ttcacccca ccacgtgac ggtcaaata atacctctt ggtgatggt atggagctg tattatggg ttctaccag tcggcaatc aggggctgg aaagcgctc ctcaacgtc gatgtcgct cataaagcg tttccgaag gcttcaaac gtcctagac atcgtgtgc gagatcgga agcgatttt aggactacc atgacacga gcaaacctt agtcaaccc ctcagagaa ttcgtccaa agagacttc gaaaaattc atcaaacag ttgaaagtc aagtacgaa atacccaac caaagtagc tccaaacgc atccatcgc gtcaacgga ctcggcgag cctcccagt caggccaaa tttaagctc gacgatggt cgaatgacc acagtcgag agatactac caagaggtg aaacgttgc aaactccaa taccctcac ctcccgact ttatgggtg ggttcgagg gaacgtgag tcgaaaatc cttctccca ttggaattt tgcactgtg gtgggtggg caagccatc aatcgcaaa atgaacgaa aatcaaacg agtgcaatg atcagaaaa gcggccact tcgaccgat gtccgcaaa gataagatc atgcagact ttacgcact gccaattat aacaatgat ccctgtatt cgcgagttt gggttttcg gtgagtaac aatttcgag aaattggat gctcgtgtt ttgaatccg ccgagtctg ttgtacgcc gataatgca caaattaag ccgagtaaa ggtgtctgg agggcggac aggaatagg tttctggtt ggggctact attaacaag tggacgatt gcgtccggg acgagatat ccgtcgaga gatgcggac aaattagct gatatgatt ttcagaatg gctagttcc aacggcatg caaatcacc tcgaaggcc actccttct acgcacatc ggcggacgg caaggcctt cgcgacttc attgactat tttaagggc aaacaagac tacgacctc atcattgtc gtggttcct aactccggc cctcagtat tctttcgtg aaacaagca gcagaactg aacgtcggc tgtctcacc caatgcatc aaggagcga accatcggc agactcaac cctcagaca gtcggaaat atcctcctc aaaatcaac agcaaaatg aacggcacg aaccaccga ctttcgccg aattcgcgt ccgctcatc atgaagcgt ccctgcatg atcatgggc gccgacgtc actcatcct agtcccgac gctcgggac atccccagc gtcgcggcg gtgacggcg tcgcacgac ccaaatgcc ttccaatac aacatctgc tggcggctg cagccaccc aaagtcgag attatcgaa gatctctgc aatatcacg gttgaacag ttgaaattc ttctatcag aagactggt tttaagccg gaaagcatc gtgtttttc cgagacggc gtctccgag ggccagttc aagcaagtc caacgagcg gaaatagcc gccatacaa aaggcgtgt aaaatgctg cagaaggac gattacgag ccgaaaatt acgttcctg gtggtgcag aaaagacac cacacgagg ctgttcccg acgaatccc cgggactcc gaagacaaa aataataat gttccagcg ggaacgtgc gtcgacacc catataacc aatccaagg atgcaggac ttttacttg gtctcacat gcgagtata caaggagtg gcaaagcct acgaagtat tgcactctt tgggatgac aataatatg aataatgac gatattgag gagctgaca taccatcta tgtcacatg tttacgagg tgcaacagg tcggtcagt tatccagca ccgacttac tatgcgcat ttggcggcg gcaagggct aaggtttat atcgaaaat gataaactg gacatgtct cagttgaaa agacaccaa gaaaaatgc caaatccag gagaaaatc gtgaaagga aaaccgatg ttttttgtt taa
- >TC013762 RA
caagttgat aagtttgtt taataataa gctcaaaat tgcttattt attgttgta tttgagttt ttatacctt tatttgtgt aaactttgt gatttaatt tgtaaaaaa aagtatcct tagactttg tagtgtagt cgtttctta ctccatttt ttatttaca ttaccgtaa tcttgaaat atcgtgaac tggaaccaa ttgtttagt gctcagtta tgggaaaga aaaaaggaa agggaaaag cccaggagg gttctggcg agccatctc agcctgcac ctggagctt ccgccccat caacatctc aaccacccc cacagcaac aacaacaga ggcaacgtg gtggccctc aaggggaag gcccagggc cacgtcagc aacatcccc aacagaggc cacaacaac agaggcaac aacaacagc ctcagccac atcctcaac aacaatggc aacaacagc ctcagccac atcctcaac aacaatggc aacaacaac agcctcagc cacatcccc aacagaggc aacagcagc agccccaac aacagtggc aacaacaac agcctcaac cacatcccc aacagtggc atcaacagc ctcagccac atcctcaac aacaatggc aacaacaac agcctcagc cacatcccc aacagaggc aacagcagc agccccaac aacagtggc aacaacaac agcctcaac cacatcccc aacagtggc atcaacaac agcctcaac agcaaagag gcccacgtc agcagcagg ggcgtccta gacagcagt ttccgccag gtattcaac agcagagga gccccagcc cacaacagc aatatgggg ctcctccgc cacactggg ggcctcccg gtattgttc cacaacaac ctatgggac aacaggttc aaggtccac ctccgcaac agcccccac gtggacctc cacctggaa tgggcgccc cccaacagc agcaacctc aacgaggtc cccttccac agcagcaac aacaacaac aacaacgtc gtccccaac aacagcaac cgcccgtta aatctcccg ttcctcatc cagaacctt cgtctcctc caagacaag aaccagcac ctcctctta gtggcggag gcgactgtc tttcgggag ctcttgttg tcacaccag gcaccaaag gcagacgta tacaaatcg aaagcaacc atttgtccc taaatctgg gcaaactca ccgaggcgt accattacg acgttgcga taacacctg acactccca aatgtttac ttcgcgatg taatgaatc tcttcggtc gcaaacact atccgcaaa accatccag ctttcgacg gcagaaaaa acctctact ctccgaaga aattaccgt ttcctaatg acaccaaaa gcgatacga ttgaagtcg aaggagaga atcgcaaaa aggagttca aagtcgagg ttaaattgg cgagaacgg tcgatttga cgcctcttc atgacatca tgaggacga cgcaatcgc cgcaggacg ccctccagt gcctggata tcgtcctcc ggaatgcac cctccaacg cttgtatca tcgccggaa ggtgctttt tcaccccac cacgtgacg gtcaaataa tacctcttg gtgatggta tggagctgt attatgggt tctaccagt cggcaatca ggggctgga aagcgctcc tcaacgtcg atgtcgctc ataaagcgt ttccgaagg cttcaaacg tcctagaca tcgtgtgcg agatcggaa gcgatttta ggactacca tgacacgag caaacctta gtcaacccc tcagagaat tcgtccaaa gagacttcg aaaaattca tcaaacagt tgaaagtca agtacgaaa tacccaacc aaagtagct ccaaacgca tccatcgcg tcaacggac tcggcgagc ctcccagtc aggccaaat ttaagctcg acgatggtc gaatgacca cagtcgaga gatactacc aagaggtga aacgttgca aactccaat accctcacc tcccgactt tatgggtgg gttcgaggg aacgtgagt cgaaaatcc ttctcccat tggaatttt gcactgtgg tgggtgggc aagccatca atcgcaaaa tgaacgaaa atcaaacga gtgcaatga tcagaaaag cggccactt cgaccgatg tccgcaaag ataagatca tgcagactt tacgcactg ccaattata acaatgatc cctgtattc gcgagtttg ggttttcgg tgagtaaca atttcgaga aattggatg ctcgtgttt tgaatccgc cgagtctgt tgtacgccg ataatgcac aaattaagc cgagtaaag gtgtctgga gggcggaca ggaataggt ttctggttg gggctacta ttaacaagt ggacgattg cgtccggga cgagatatc cgtcgagag atgcggaca aattagctg atatgattt tcagaatgg ctagttcca acggcatgc aaatcacct cgaaggcca ctccttcta cgcacatcg gcggacggc aaggccttc gcgacttca ttgactatt ttaagggca aacaagact acgacctca tcattgtcg tggttccta actccggcc ctcagtatt ctttcgtga aacaagcag cagaactga acgtcggct gtctcaccc aatgcatca aggagcgaa ccatcggca gactcaacc ctcagacag tcggaaata tcctcctca aaatcaaca gcaaaatga acggcacga accaccgac tttcgccga attcgcgtc cgctcatca tgaagcgtc cctgcatga tcatgggcg ccgacgtca ctcatccta gtcccgacg ctcgggaca tccccagcg tcgcggcgg tgacggcgt cgcacgacc caaatgcct tccaataca acatctgct ggcggctgc agccaccca aagtcgaga ttatcgaag atctctgca atatcacgg ttgaacagt tgaaattct tctatcaga agactggtt ttaagccgg aaagcatcg tgtttttcc gagacggcg tctccgagg gccagttca agcaagtcc aacgagcgg aaatagccg ccatacaaa aggcgtgta aaatgctgc agaaggacg attacgagc cgaaaatta cgttcctgg tggtgcaga aaagacacc acacgaggc tgttcccga cgaatcccc gggactccg aagacaaaa ataataatg ttccagcgg gaacgtgcg tcgacaccc atataacca atccaagga tgcaggact tttacttgg tctcacatg cgagtatac aaggagtgg caaagccta cgaagtatt gcactcttt gggatgaca ataatatga ataatgacg atattgagg agctgacat accatctat gtcacatgt ttacgaggt gcaacaggt cggtcagtt atccagcac cgacttact atgcgcatt tggcggcgg caagggcta aggtttata tcgaaaatg ataaactgg acatgtctc agttgaaaa gacaccaag aaaaatgcc aaatccagg agaaaatcg tgaaaggaa aaccgatgt tttttgttt aaaggaact ggagtactg tttatcatg ttggtaaaa ttttctatg taaggcttt atatctatt caatgtttg tttattaat tattatccc aaaaaaatt ataaaaatt atggtaaaa taaaaaatg cgttgcgcg
