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Gene information
General information
TC013752 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4811604..4820776
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013752
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05491

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013752 RA
      MEKYSESQL PSKLQPSEN ASFLSKLFF CWAPKFFYD 
      GYTKHPTEE YYATVKSHS SCYLAGKLE KSWERASPS 
      LWKALWKTF HFEFVALCL LFIISEFTF KISQPWLVA 
      KLMEEIINS KNEYYLYGF LVILVNFLS VIVGHFYHL 
      KVQHLGMKI RISCCSLIY QKALKISKQ VESESKMGK 
      IVNLLSNDV NRFDLAPMH LINLIVAPF ETLFVIFSL 
      YATVGTTAV SGIVFLAVF MPLQMYMGK LTTTYRLKS 
      ATNTDRRIK LMNEIITGI KVIKMFVWE KLFVDIIEK 
      ARRLEVRQI RKISNIRAL NVSFMLFIN RTGIFLSVM 
      TYFLVRKQV DAKYVFVLS SFYAILRQT LTVYLPFAI 
      QNFSETRVS VERIRLFLT CDEQIAVTY VASDKEQLV 
      KRDITIQEF GNIEGTKVG IRLKNASVK WLLAENYSL 
      NSVNFEVFG EFVIVSGPV GGGKSTLLY TILKELPLD 
      QGELSVKGI LSYMSQEPW LFSGTVQQN ILFGSKFDK 
      NKYNTIVQI CQLEADLST FPYGDHTLV GENGALLSG 
      GQKTRINLA RALYSDADI YLLDDPFAS VDTIIGKKI 
      FQDCILTYL RNKCVVLVT NQQGFFAAA DRVYTLDKG 
      ILTNNVNIY KKEYTFEEQ QSREHIISG EKITELKLE 
      PKKGRQGNV TRQVYAKYA KSAGNCSSH CLLFFLFLA 
      SQIAASGAD YFVAFWVNL SQSSTRNSF TDDICLQIY 
      LALIIATIT LSIARSITF FRLCIKASV KLHNCMFAK 
      VIKAPITFF ETNPSGEIL NRFSKDIGM VDETIPSIL 
      MDTFQIAFI ILGSVVLII FLNPWMLIP TLVIFPLFY 
      FFKIWFLKR SRNLKRLEA SARTPIYTH VRETLKGLT 
      VIRTYNAEI TTKRQFSCY QDLHSSAFY TFMTCNRAF 
      GFWLDLICM FYTIGVIAS IMLMETQAG SVGLSITQS 
      INLIGVLQW GIKQWSELE NQMINVERV SEYTEISPE 
      QSLTKNLGE IWPSEGQIK FQSVSMRYP QSGRLTLDN 
      ISFTVEPRE KIGIIGRTG AGKSSLVST LFRLYNFEG 
      KILVDGVNT CEIPLDTLR SKISIIPQE PILFTGTLR 
      ENLDPFGEF PDAVLWNVL EQVKLKSVV ANFSDGLSV 
      QVLEGGSNF SVGQKQLIC LGRAILRKN KILILDEAT 
      ANIDFQTDR FIQETVQSE FKNCTVLTV AHRINTVMD 
      SDKIMVLDD GKLVEFDSP SSLLRNSDG LFRQVVDEC 
      KESLSKAK
    CDS sequence
    • >TC013752 RA
      atggaaaaa tatagtgaa tcgcaactg ccttcaaaa 
      ctccaacct agtgaaaat gcgtccttt ctatcgaaa 
      ctatttttt tgttgggca ccaaaattt ttttacgac 
      ggctacacc aaacatccc actgaagaa tactacgcc 
      actgttaaa agccacagc tcgtgttac ttagccgga 
      aaattggaa aaatcatgg gaaagagcg agcccgtcc 
      ctgtggaaa gcactctgg aaaacgttt cactttgaa 
      ttcgtagcc ttatgtctc ttatttatt atctcagag 
      tttacgttc aagataagc caaccatgg ctcgttgca 
      aaactcatg gaggagatc atcaattcg aaaaacgag 
      tactatttg tatggtttt ttagtcatt ctagtgaac 
      tttttaagt gtgatagtg ggccatttc taccacctc 
      aaggtccaa catttaggg atgaaaatc cgcattagt 
      tgttgttct ctgatttac cagaaagct ctaaagatt 
      agtaagcaa gtggagtcg gagtcgaaa atgggaaaa 
      atcgttaac ttgttatca aatgatgtt aaccgcttt 
      gatttggcg ccgatgcac ttgatcaac ctcatcgta 
      gcaccgttt gagacactt tttgtgata tttagcttg 
      tatgcaacg gttggcacc acagcggtc agcggaatc 
      gttttttta gcagtattt atgccactt caaatgtac 
      atggggaaa ttaacgacg acttataga ctgaaaagt 
      gccacaaac accgaccga aggatcaaa ctaatgaac 
      gaaattatc acaggcatc aaagtgata aaaatgttc 
      gtttgggaa aaactattt gtggacatc atcgaaaaa 
      gccagaagg ttggaagtt cgccaaata cgcaaaata 
      tcaaacatt cgagcgttg aacgtctcc ttcatgctt 
      ttcataaac cggactggg attttctta tccgtcatg 
      acgtacttt ctggtccgg aagcaagtc gatgccaaa 
      tacgtgttt gtcctgtcg tcgttttac gcaattttg 
      cgacaaact ctaacagtt tacctgccc tttgctatc 
      cagaatttc agcgaaact agggtttct gtcgagaga 
      attcgcctc ttcctaacg tgcgatgaa caaattgca 
      gtgacttat gtagcctca gataaggaa caactggtg 
      aaacgcgat atcaccatt caggaattt gggaatatt 
      gagggaacg aaagttggg attcggttg aaaaacgcc 
      tcagtgaag tggctactg gccgaaaat tacagcttg 
      aacagcgtg aattttgag gtttttggg gagtttgtg 
      atagttagt gggccagtg ggtggtgga aaatccact 
      cttctgtac acgatttta aaagaactg ccccttgat 
      caaggagag ttgagtgtg aaaggcatt ctctcgtat 
      atgtctcaa gagccgtgg ctgttttcg ggcacagtt 
      caacaaaac atactgttt gggtcaaaa tttgacaaa 
      aataagtac aacacaatc gtgcaaatt tgccagttg 
      gaagcggac ctctccact tttccgtac ggtgaccac 
      actttagtt ggggaaaac ggggctctg ttaagtgga 
      ggccagaaa acgcggatc aatttagct cgggccctt 
      tacagtgac gccgatatt tatctactg gacgatcct 
      tttgcgtcc gttgacacg atcatcggg aagaaaatt 
      ttccaagat tgcattctc acgtactta aggaacaaa 
      tgtgtggtt ctagtcaca aatcagcaa ggttttttc 
      gccgcagct gatagagtt tacacttta gataagggt 
      atcctcacc aataatgta aatatttac aagaaagag 
      tacactttt gaggagcaa caaagtagg gagcatata 
      ataagtggg gaaaaaatc acagaactg aaactggaa 
      cccaaaaaa gggaggcaa gggaatgtc acgagacaa 
      gtgtatgca aaatatgcc aaatcggcc gggaattgc 
      tcgagtcat tgtctcctc ttttttctg tttctggcg 
      tcgcaaatt gcggccagt ggggccgac tattttgtg 
      gcattttgg gttaatttg agccaatct tccactcgc 
      aattctttc accgacgat atttgtctc caaatttat 
      ctcgctctt attattgca accataact ttatcaatc 
      gcgagatct atcacattt ttccgattg tgcatcaaa 
      gcttcagta aaattgcac aattgcatg tttgccaaa 
      gtaatcaaa gcccccata actttcttc gaaacgaat 
      ccttcgggg gaaatactg aaccgtttc tcgaaagac 
      atcggcatg gtcgatgag accattcct tccattctc 
      atggatacg tttcaaatc gctttcatt atcctagga 
      agtgtggtt ttgataatt ttcctaaat ccttggatg 
      ctgatacca acactagtg atttttccc ctgttctat 
      ttttttaaa atttggttt ctaaaaaga agtcgcaat 
      ctaaaacgg ctggaagct tcagcacgc actccaatc 
      tacacgcac gttcgtgaa accctcaaa ggtttaacg 
      gtgatcagg acatacaat gcggaaatc acaaccaaa 
      cgccaattc agctgctac caagacttg cacagctcg 
      gctttttac actttcatg acttgcaat cgagctttt 
      ggcttctgg ttggactta atctgcatg ttttacacc 
      attggggtg atagcgagc atcatgttg atggagaca 
      caagccggc agtgttggc ttgagtata acgcagtca 
      ataaacctg atcggggtg ctccagtgg ggcatcaaa 
      cagtggagc gagctggaa aaccagatg atcaacgtt 
      gaacgtgtc tctgaatac acggagatt tcccccgag 
      caaagcctg acgaaaaat ttgggcgaa atttggcca 
      agtgaaggc caaatcaag tttcaatcc gtctcaatg 
      cgctacccc cagagcggt cgtctaacc ctggacaac 
      ataagcttc acggtcgaa cctagagag aaaatcgga 
      ataattggg cgaaccggc gctggaaaa tcatcccta 
      gtttcaacc ctcttccgt ttgtacaac ttcgagggg 
      aaaattctc gtcgacgga gtcaacact tgcgaaatt 
      ccactcgac accctccgg tcaaaaatc tcaatcatc 
      ccccaggaa ccgatcctc ttcacaggg acgctgcgg 
      gagaatctg gaccccttt ggcgagttt cccgacgcc 
      gttttgtgg aacgttctg gagcaagtg aaactgaaa 
      agtgtggta gcgaacttt tcagacggc ctatcggtt 
      caagtcctg gaaggaggg tcgaatttc agcgtggga 
      cagaagcag ctgatttgt ctaggaaga gcgatcttg 
      aggaagaac aagattctt attttggat gaagcgacc 
      gcaaatatc gatttccaa acggatcgg ttcattcaa 
      gaaacggtg cagagtgaa ttcaagaac tgcaccgtt 
      ttaacggtg gcccatagg atcaatacg gttatggat 
      tcggacaag attatggtc ctggatgat gggaaattg 
      gtggagttt gatagtccg agcagtttg ttgcggaat 
      tcggacggg ttgtttcgg caagtggtg gatgagtgt 
      aaagaaagt ttgtccaaa gcgaaataa
    mRNA sequence
    • >TC013752 RA
      ctacttagt taaatgttt taaatgatt tttagtgtt 
      ttggaacga attataaca tacaatata aattataaa 
      taattacct attgttgta attattaaa taggtaaat 
      atttgagta caatcaaca atttatata aaaacaatt 
      acaaaaaaa tatgtgata cataatttt aaacaagta 
      atttccaat caacttact ggttactgt tgtaactaa 
      acctgcaga tcaaaatca tcaaatctt gccacatta 
      cgattctaa tattacttt aactcttca agaagtttc 
      agtttttag tgaaactgg tgaaatggt gaaacttgc 
      actgaaact tttcaagct cacaatact tatttcccc 
      gttccgtgg ctaaataaa ttctgacga cgccactgg 
      gtagataat taaaacact cacctactt gcctaaaac 
      gcaattttc acctacttt cgccaaata aatttcaat 
      aaaatacta agctacaat aaactgtta tcagtgaga 
      gacaacctt gaaagttgg aatgttact taacgaaac 
      aatggactt atagtagag atccctgtt tatgtagta 
      ttgtactta aaaatcagc tttaatctc aatgaagaa 
      tgtatactt ggttcaagt cagttcggt tttagtgaa 
      aatacagtt catagttac agaccactc gatcactac 
      tattttttg aaccaaaat aacgttaat ggaaaaata 
      tagtgaatc gcaactgcc ttcaaaact ccaacctag 
      tgaaaatgc gtcctttct atcgaaact atttttttg 
      ttgggcacc aaaattttt ttacgacgg ctacaccaa 
      acatcccac tgaagaata ctacgccac tgttaaaag 
      ccacagctc gtgttactt agccggaaa attggaaaa 
      atcatggga aagagcgag cccgtccct gtggaaagc 
      actctggaa aacgtttca ctttgaatt cgtagcctt 
      atgtctctt atttattat ctcagagtt tacgttcaa 
      gataagcca accatggct cgttgcaaa actcatgga 
      ggagatcat caattcgaa aaacgagta ctatttgta 
      tggtttttt agtcattct agtgaactt tttaagtgt 
      gatagtggg ccatttcta ccacctcaa ggtccaaca 
      tttagggat gaaaatccg cattagttg ttgttctct 
      gatttacca gaaagctct aaagattag taagcaagt 
      ggagtcgga gtcgaaaat gggaaaaat cgttaactt 
      gttatcaaa tgatgttaa ccgctttga tttggcgcc 
      gatgcactt gatcaacct catcgtagc accgtttga 
      gacactttt tgtgatatt tagcttgta tgcaacggt 
      tggcaccac agcggtcag cggaatcgt ttttttagc 
      agtatttat gccacttca aatgtacat ggggaaatt 
      aacgacgac ttatagact gaaaagtgc cacaaacac 
      cgaccgaag gatcaaact aatgaacga aattatcac 
      aggcatcaa agtgataaa aatgttcgt ttgggaaaa 
      actatttgt ggacatcat cgaaaaagc cagaaggtt 
      ggaagttcg ccaaatacg caaaatatc aaacattcg 
      agcgttgaa cgtctcctt catgctttt cataaaccg 
      gactgggat tttcttatc cgtcatgac gtactttct 
      ggtccggaa gcaagtcga tgccaaata cgtgtttgt 
      cctgtcgtc gttttacgc aattttgcg acaaactct 
      aacagttta cctgccctt tgctatcca gaatttcag 
      cgaaactag ggtttctgt cgagagaat tcgcctctt 
      cctaacgtg cgatgaaca aattgcagt gacttatgt 
      agcctcaga taaggaaca actggtgaa acgcgatat 
      caccattca ggaatttgg gaatattga gggaacgaa 
      agttgggat tcggttgaa aaacgcctc agtgaagtg 
      gctactggc cgaaaatta cagcttgaa cagcgtgaa 
      ttttgaggt ttttgggga gtttgtgat agttagtgg 
      gccagtggg tggtggaaa atccactct tctgtacac 
      gattttaaa agaactgcc ccttgatca aggagagtt 
      gagtgtgaa aggcattct ctcgtatat gtctcaaga 
      gccgtggct gttttcggg cacagttca acaaaacat 
      actgtttgg gtcaaaatt tgacaaaaa taagtacaa 
      cacaatcgt gcaaatttg ccagttgga agcggacct 
      ctccacttt tccgtacgg tgaccacac tttagttgg 
      ggaaaacgg ggctctgtt aagtggagg ccagaaaac 
      gcggatcaa tttagctcg ggcccttta cagtgacgc 
      cgatattta tctactgga cgatccttt tgcgtccgt 
      tgacacgat catcgggaa gaaaatttt ccaagattg 
      cattctcac gtacttaag gaacaaatg tgtggttct 
      agtcacaaa tcagcaagg ttttttcgc cgcagctga 
      tagagttta cactttaga taagggtat cctcaccaa 
      taatgtaaa tatttacaa gaaagagta cacttttga 
      ggagcaaca aagtaggga gcatataat aagtgggga 
      aaaaatcac agaactgaa actggaacc caaaaaagg 
      gaggcaagg gaatgtcac gagacaagt gtatgcaaa 
      atatgccaa atcggccgg gaattgctc gagtcattg 
      tctcctctt ttttctgtt tctggcgtc gcaaattgc 
      ggccagtgg ggccgacta ttttgtggc attttgggt 
      taatttgag ccaatcttc cactcgcaa ttctttcac 
      cgacgatat ttgtctcca aatttatct cgctcttat 
      tattgcaac cataacttt atcaatcgc gagatctat 
      cacattttt ccgattgtg catcaaagc ttcagtaaa 
      attgcacaa ttgcatgtt tgccaaagt aatcaaagc 
      ccccataac tttcttcga aacgaatcc ttcggggga 
      aatactgaa ccgtttctc gaaagacat cggcatggt 
      cgatgagac cattccttc cattctcat ggatacgtt 
      tcaaatcgc tttcattat cctaggaag tgtggtttt 
      gataatttt cctaaatcc ttggatgct gataccaac 
      actagtgat ttttcccct gttctattt ttttaaaat 
      ttggtttct aaaaagaag tcgcaatct aaaacggct 
      ggaagcttc agcacgcac tccaatcta cacgcacgt 
      tcgtgaaac cctcaaagg tttaacggt gatcaggac 
      atacaatgc ggaaatcac aaccaaacg ccaattcag 
      ctgctacca agacttgca cagctcggc tttttacac 
      tttcatgac ttgcaatcg agcttttgg cttctggtt 
      ggacttaat ctgcatgtt ttacaccat tggggtgat 
      agcgagcat catgttgat ggagacaca agccggcag 
      tgttggctt gagtataac gcagtcaat aaacctgat 
      cggggtgct ccagtgggg catcaaaca gtggagcga 
      gctggaaaa ccagatgat caacgttga acgtgtctc 
      tgaatacac ggagatttc ccccgagca aagcctgac 
      gaaaaattt gggcgaaat ttggccaag tgaaggcca 
      aatcaagtt tcaatccgt ctcaatgcg ctaccccca 
      gagcggtcg tctaaccct ggacaacat aagcttcac 
      ggtcgaacc tagagagaa aatcggaat aattgggcg 
      aaccggcgc tggaaaatc atccctagt ttcaaccct 
      cttccgttt gtacaactt cgaggggaa aattctcgt 
      cgacggagt caacacttg cgaaattcc actcgacac 
      cctccggtc aaaaatctc aatcatccc ccaggaacc 
      gatcctctt cacagggac gctgcggga gaatctgga 
      cccctttgg cgagtttcc cgacgccgt tttgtggaa 
      cgttctgga gcaagtgaa actgaaaag tgtggtagc 
      gaacttttc agacggcct atcggttca agtcctgga 
      aggagggtc gaatttcag cgtgggaca gaagcagct 
      gatttgtct aggaagagc gatcttgag gaagaacaa 
      gattcttat tttggatga agcgaccgc aaatatcga 
      tttccaaac ggatcggtt cattcaaga aacggtgca 
      gagtgaatt caagaactg caccgtttt aacggtggc 
      ccataggat caatacggt tatggattc ggacaagat 
      tatggtcct ggatgatgg gaaattggt ggagtttga 
      tagtccgag cagtttgtt gcggaattc ggacgggtt 
      gtttcggca agtggtgga tgagtgtaa agaaagttt 
      gtccaaagc gaaataaaa gagtaaatt tctatgttc 
      acaaaaacc tttttttta attttatta acaaacgta 
      aaggcctca acacgagcc ctcctacca tcttggcaa 
      taaaataaa atctgagta tttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY