Status
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Gene information
General information
TC013751 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4823999..4833552
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013751
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05490

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    low validity due to technical reasons - should be repeated: pupal phenotype not scorable,
    5 of six animals were alreada adults.
    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    low validity due to technical reasons - should be repeated: pupal phenotype not scorable,
    5 of six animals were alreada adults.
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013751 RA
      MESTEKTID GCKKKTHPH KKANIISKL FFCWPLPLF 
      VKGFRKDLT EDDLYGTLK AHKSNLLGD KLEKKWIKE 
      TNNHRNPSL WRVLFQVYG LETILYGVV LLIQELIVK 
      MAHPMLIGG LMAYYDPNQ VEITKQMAY LYSAGIIGI 
      SFLNIMIMH SYFFGLQQL GMKIRVSCC SLLYRKALR 
      LSKSSLIDT TIGQTVNLM SNDVNRFDF LIMHIHHLV 
      IAPLEALVV IYLLYTTVH PAALAGAGL MVIFVPLQL 
      YLGKRTSFY RYRTAIKTD QRVRLMNEI ITGIQVIKM 
      YTWEKPFAK LVEMARKLE IHQIRASSY LKAINVSFI 
      IFLNRTSIY LCILTYVLT GNSLNAGYV YVVTSYYGI 
      LRQSLTMFL PRGITLLAE TNVSVKRIQ KFLSYDEIK 
      PQVNHPDPE KPIGVYMED ISVRWSPTT PDFTLSGVN 
      FSVGPQHLV GVVGPVGSG KTTLLHVIL KEIALAKGN 
      LEISGRISY AAQEPWLFA ASIRQNILF GEKMDREKY 
      QQVVKVCAL ERDFSMFPY GDHTIVGER GVMLSGGQK 
      ARINLARAV YKDADIYLL DDPLSAVDT HVGKRLFED 
      CISGYLREK CTVLVTHQL QYLRNVDRI YLLEGGAIT 
      ASGTFSELQ NSDSEFVKL LEKLVTDED KHDKQEETS 
      QKLKAFKSF DKEKPSEVK EHRSVGTLS KRIYFCYLK 
      AAGNYCLSV CILLLFVFA QMAASGTDV FVTFWVNLE 
      QDRSSNVTN VVSAFFTPD NCIYIHSSF IVFLIVVTI 
      TRSLSFFKV CMRASRNLH NNMFLSIVH ATMRFFNTN 
      PSGRILNRF SKDMGSIDE TLPQSITDT LQVGLNVFS 
      ITIVLSTIN PWIIIPTVI IFAVFYMFK VIFLATSRN 
      LKRMEGTTR SPVFSHLSQ SLQGLSTIR AFNAQETLR 
      LEFDNHQDL HSSTYHMFI ATSRTFAFW LDINCILYI 
      SIVILSFLF IGVESYGGN VGLAITQSI TLTGMLQWG 
      MRQWSELEN QMTSVERVI EYTELEQES DEKRKNVPE 
      TWPTSGRIE FQSVFMQYS PDDPFVLKN LTFVINSKE 
      KIGIVGRTG AGKSSIISA LFRLVPTEG NIIIDGVDI 
      NEISLHCLR SNISIIPQE PILFSGTLR KNLDPFDEY 
      SDEQLWKAL DEVKLKALV SEQPSGLAS NVSEGGSNF 
      SVGQRQLLC LARAVIRNN VILVLDEAT ANVDPQTDE 
      LIQNTIRRK FKECTVLTI AHRLHTVMD SDKILVMSG 
      GRAVEFDEP YSLLQKTDG VFYGLVQQT GKGMAENLM 
      NIANKNREY TTETDS
    CDS sequence
    • >TC013751 RA
      atggaaagc accgaaaaa accatcgat gggtgcaag 
      aagaagaca cacccgcac aaaaaagcc aacattatt 
      tcaaaacta ttcttctgc tggcccttg ccattgttc 
      gtcaaaggc ttcaggaaa gacctcacc gaggacgac 
      ctatacggc actttgaaa gcacacaag tccaacctt 
      ttgggcgac aaactcgaa aagaaatgg atcaaggag 
      acgaataat cacagaaac ccgtccttg tggcgagtt 
      ttattccaa gtgtatggg cttgaaacc atactctat 
      ggagttgtt ttactcatc caggaatta atcgtaaaa 
      atggctcat ccgatgcta ataggcggg cttatggcc 
      tattacgac cccaaccaa gtggagatc accaaacag 
      atggcctat ttgtactct gctgggatc attggaata 
      tcattcttg aacataatg atcatgcat tcgtatttc 
      tttggcttg caacagctc gggatgaaa atccgggtc 
      tcttgctgt tccctcctt taccggaag gctctcaga 
      ctgagtaaa agcagtctg attgataca acaatagga 
      cagactgtt aatctcatg tccaatgat gtgaaccgg 
      ttcgatttc ctgataatg cacatacac cacttggtg 
      atagcacca ctggaagcc ctggttgtg atttacctg 
      ctctacacc actgtccat ccggcagcg cttgcagga 
      gcgggcctt atggtcatt tttgtgcct ttgcaactg 
      tatttgggc aaacgcacg tccttctac cggtacaga 
      acggcgata aaaaccgac caaagagtg agacttatg 
      aacgaaatc atcaccgga atacaagtc atcaaaatg 
      tacacgtgg gaaaaaccg ttcgccaaa ctggtcgaa 
      atggcccga aaattggaa atccaccaa atccgggcc 
      agctcctac ctcaaagcg atcaacgtc tcatttatc 
      attttcctg aaccggact tccatctac ctctgcatt 
      ctaacatac gttctgacc ggtaactcg ctaaatgcc 
      ggttacgtc tacgttgtt acgtcctac tacggcatt 
      ttgcgacaa tccctgacc atgtttctg ccccgaggg 
      atcacactt ctggccgaa accaacgtt tcggtgaaa 
      cgaatccag aaattcctg tcgtacgat gagatcaag 
      ccccaggtg aatcaccca gaccccgag aaacccatc 
      ggcgtttac atggaggac atcagcgtc agatggtca 
      ccaaccaca ccggacttc accctcagc ggagtcaac 
      ttcagtgtg ggtccccag catctggtg ggtgtcgtg 
      gggcctgtg gggagtggc aaaaccacc ctcttgcac 
      gttatcctg aaggaaatc gctttggcg aagggcaat 
      ctggagata agtggtcgc atctcgtat gctgcgcag 
      gaaccgtgg cttttcgca gccagtatc cggcagaac 
      atccttttc ggcgagaaa atggaccgg gagaagtac 
      caacaagtg gtgaaagtg tgcgcgctg gagcgggac 
      ttttccatg tttccttac ggggaccac acgattgtg 
      ggggagcgt ggtgttatg ttaagtggc ggccagaag 
      gcgcggatc aatttggcg agggcggtt tacaaagac 
      gctgatatt tacctgctt gatgatccg ctctcggcg 
      gtggacacg cacgtgggg aagaggctc ttcgaggat 
      tgcatctcg ggttatcta agggagaag tgcacggtt 
      ttggtcaca caccagctg cagtacctc cgcaatgtg 
      gacaggatc tacttgctt gaaggcggt gccatcaca 
      gcgagtggc accttctca gaattgcaa aactccgac 
      tcagagttc gtcaagttg ttggagaag ctggtcact 
      gatgaagac aagcacgac aagcaggag gaaaccagc 
      cagaagctc aaagctttc aaaagcttc gataaggag 
      aagccgagc gaagttaag gagcaccgc agtgttggc 
      accctctcg aagcgaatc tacttttgt tacttgaaa 
      gcagcgggg aattactgc ttgtcggtg tgcattttg 
      ttgttgttc gttttcgcg caaatggcg gcaagtggg 
      acggatgtg ttcgtcacg ttttgggtc aatctggag 
      caggacagg tcgagcaac gtgacgaat gtggtttct 
      gcgtttttt acacccgat aactgcatt tatatacac 
      tcttcgttt atagttttt ctcattgtt gtaactata 
      acgcgctct ttaagcttc ttcaaagtg tgcatgagg 
      gcttccaga aatttgcat aataacatg tttttgagc 
      attgtgcat gcgacgatg aggtttttc aatactaat 
      ccctcggga aggattttg aacaggttt tccaaggat 
      atggggtcc attgacgag acgcttcca cagtcaatt 
      accgacact ttacaggtc ggtttgaac gtgttttcg 
      atcacaatc gtcttgtcg acaatcaat ccatggatt 
      atcatcccg actgtcatc attttcgcg gtgttttac 
      atgtttaaa gtcatattt ttggccaca agcaggaac 
      ttaaagcga atggaagga acaactcga agtccggtt 
      ttctctcac ctatctcaa tcactacaa ggcctgtca 
      acaatccga gctttcaac gcccaagaa acccttcga 
      ttagaattc gacaatcac caagacctt cacagctcc 
      acatatcac atgtttatc gccaccagt cgaacattt 
      gcattttgg ttggacatc aactgcatt ttgtacatc 
      agtatcgta atccttagt tttctcttc atcggtgtt 
      gagtcatac ggtggaaac gtcggtttg gcaataacc 
      caatcaatc acactgacc ggaatgttg caatggggc 
      atgagacag tggagcgag ctcgaaaac caaatgact 
      tcggtggaa cgagtgatc gaatacacc gaacttgag 
      caagaatcg gacgaaaaa cgcaaaaac gtgcccgaa 
      acctggccc acaagtgga agaattgag ttccaatca 
      gtcttcatg cagtactct cctgacgat ccctttgtt 
      ttgaaaaac ctgactttt gtgatcaat tcgaaggaa 
      aaaatcggg attgttggg cgaacagga gctggaaaa 
      tcgtccatc atttcggcc cttttccgc ctggtgccc 
      accgaaggc aatataata atcgacggt gtggacatt 
      aacgaaatc tccttgcac tgcttgagg tccaacatc 
      tcgatcatc ccgcaggaa ccgatcctt ttttcgggg 
      actttgcgc aagaatctg gaccccttc gacgaatac 
      agtgacgaa cagctttgg aaagctcta gacgaggtt 
      aagttgaaa gccctcgtt tcggaacaa ccctcagga 
      ttggcgagc aatgtgtcg gaaggcggg tcgaatttc 
      agtgtgggc cagagacag ttgctttgc cttgccaga 
      gcggttatt cggaataac gtcattctg gtgctggac 
      gaagccacg gcgaacgtc gacccacaa accgacgaa 
      ctgatccag aacacgatc aggaggaag ttcaaggaa 
      tgcaccgtc ttgaccatc gcacacaga cttcatacg 
      gtgatggat tcggacaag attttggtc atgagtgga 
      ggacgagct gtagaattt gatgagccg tactctctt 
      ttgcagaaa acggacgga gtgttttac ggattggtg 
      cagcaaacg gggaaagga atggccgag aatttgatg 
      aatattgca aacaagaat agagaatac accacagaa 
      acggattca taa
    mRNA sequence
    • >TC013751 RA
      tgtgattgt gcaagctga cataaatat tcaattttc 
      aaatttaaa tcaccgcgc aaaatggaa agcaccgaa 
      aaaaccatc gatgggtgc aagaagaag acacacccg 
      cacaaaaaa gccaacatt atttcaaaa ctattcttc 
      tgctggccc ttgccattg ttcgtcaaa ggcttcagg 
      aaagacctc accgaggac gacctatac ggcactttg 
      aaagcacac aagtccaac cttttgggc gacaaactc 
      gaaaagaaa tggatcaag gagacgaat aatcacaga 
      aacccgtcc ttgtggcga gttttattc caagtgtat 
      gggcttgaa accatactc tatggagtt gttttactc 
      atccaggaa ttaatcgta aaaatggct catccgatg 
      ctaataggc gggcttatg gcctattac gaccccaac 
      caagtggag atcaccaaa cagatggcc tatttgtac 
      tctgctggg atcattgga atatcattc ttgaacata 
      atgatcatg cattcgtat ttctttggc ttgcaacag 
      ctcgggatg aaaatccgg gtctcttgc tgttccctc 
      ctttaccgg aaggctctc agactgagt aaaagcagt 
      ctgattgat acaacaata ggacagact gttaatctc 
      atgtccaat gatgtgaac cggttcgat ttcctgata 
      atgcacata caccacttg gtgatagca ccactggaa 
      gccctggtt gtgatttac ctgctctac accactgtc 
      catccggca gcgcttgca ggagcgggc cttatggtc 
      atttttgtg cctttgcaa ctgtatttg ggcaaacgc 
      acgtccttc taccggtac agaacggcg ataaaaacc 
      gaccaaaga gtgagactt atgaacgaa atcatcacc 
      ggaatacaa gtcatcaaa atgtacacg tgggaaaaa 
      ccgttcgcc aaactggtc gaaatggcc cgaaaattg 
      gaaatccac caaatccgg gccagctcc tacctcaaa 
      gcgatcaac gtctcattt atcattttc ctgaaccgg 
      acttccatc tacctctgc attctaaca tacgttctg 
      accggtaac tcgctaaat gccggttac gtctacgtt 
      gttacgtcc tactacggc attttgcga caatccctg 
      accatgttt ctgccccga gggatcaca cttctggcc 
      gaaaccaac gtttcggtg aaacgaatc cagaaattc 
      ctgtcgtac gatgagatc aagccccag gtgaatcac 
      ccagacccc gagaaaccc atcggcgtt tacatggag 
      gacatcagc gtcagatgg tcaccaacc acaccggac 
      ttcaccctc agcggagtc aacttcagt gtgggtccc 
      cagcatctg gtgggtgtc gtggggcct gtggggagt 
      ggcaaaacc accctcttg cacgttatc ctgaaggaa 
      atcgctttg gcgaagggc aatctggag ataagtggt 
      cgcatctcg tatgctgcg caggaaccg tggcttttc 
      gcagccagt atccggcag aacatcctt ttcggcgag 
      aaaatggac cgggagaag taccaacaa gtggtgaaa 
      gtgtgcgcg ctggagcgg gacttttcc atgtttcct 
      tacggggac cacacgatt gtgggggag cgtggtgtt 
      atgttaagt ggcggccag aaggcgcgg atcaatttg 
      gcgagggcg gtttacaaa gacgctgat atttacctg 
      cttgatgat ccgctctcg gcggtggac acgcacgtg 
      gggaagagg ctcttcgag gattgcatc tcgggttat 
      ctaagggag aagtgcacg gttttggtc acacaccag 
      ctgcagtac ctccgcaat gtggacagg atctacttg 
      cttgaaggc ggtgccatc acagcgagt ggcaccttc 
      tcagaattg caaaactcc gactcagag ttcgtcaag 
      ttgttggag aagctggtc actgatgaa gacaagcac 
      gacaagcag gaggaaacc agccagaag ctcaaagct 
      ttcaaaagc ttcgataag gagaagccg agcgaagtt 
      aaggagcac cgcagtgtt ggcaccctc tcgaagcga 
      atctacttt tgttacttg aaagcagcg gggaattac 
      tgcttgtcg gtgtgcatt ttgttgttg ttcgttttc 
      gcgcaaatg gcggcaagt gggacggat gtgttcgtc 
      acgttttgg gtcaatctg gagcaggac aggtcgagc 
      aacgtgacg aatgtggtt tctgcgttt tttacaccc 
      gataactgc atttatata cactcttcg tttatagtt 
      tttctcatt gttgtaact ataacgcgc tctttaagc 
      ttcttcaaa gtgtgcatg agggcttcc agaaatttg 
      cataataac atgtttttg agcattgtg catgcgacg 
      atgaggttt ttcaatact aatccctcg ggaaggatt 
      ttgaacagg ttttccaag gatatgggg tccattgac 
      gagacgctt ccacagtca attaccgac actttacag 
      gtcggtttg aacgtgttt tcgatcaca atcgtcttg 
      tcgacaatc aatccatgg attatcatc ccgactgtc 
      atcattttc gcggtgttt tacatgttt aaagtcata 
      tttttggcc acaagcagg aacttaaag cgaatggaa 
      ggaacaact cgaagtccg gttttctct cacctatct 
      caatcacta caaggcctg tcaacaatc cgagctttc 
      aacgcccaa gaaaccctt cgattagaa ttcgacaat 
      caccaagac cttcacagc tccacatat cacatgttt 
      atcgccacc agtcgaaca tttgcattt tggttggac 
      atcaactgc attttgtac atcagtatc gtaatcctt 
      agttttctc ttcatcggt gttgagtca tacggtgga 
      aacgtcggt ttggcaata acccaatca atcacactg 
      accggaatg ttgcaatgg ggcatgaga cagtggagc 
      gagctcgaa aaccaaatg acttcggtg gaacgagtg 
      atcgaatac accgaactt gagcaagaa tcggacgaa 
      aaacgcaaa aacgtgccc gaaacctgg cccacaagt 
      ggaagaatt gagttccaa tcagtcttc atgcagtac 
      tctcctgac gatcccttt gttttgaaa aacctgact 
      tttgtgatc aattcgaag gaaaaaatc gggattgtt 
      gggcgaaca ggagctgga aaatcgtcc atcatttcg 
      gcccttttc cgcctggtg cccaccgaa ggcaatata 
      ataatcgac ggtgtggac attaacgaa atctccttg 
      cactgcttg aggtccaac atctcgatc atcccgcag 
      gaaccgatc cttttttcg gggactttg cgcaagaat 
      ctggacccc ttcgacgaa tacagtgac gaacagctt 
      tggaaagct ctagacgag gttaagttg aaagccctc 
      gtttcggaa caaccctca ggattggcg agcaatgtg 
      tcggaaggc gggtcgaat ttcagtgtg ggccagaga 
      cagttgctt tgccttgcc agagcggtt attcggaat 
      aacgtcatt ctggtgctg gacgaagcc acggcgaac 
      gtcgaccca caaaccgac gaactgatc cagaacacg 
      atcaggagg aagttcaag gaatgcacc gtcttgacc 
      atcgcacac agacttcat acggtgatg gattcggac 
      aagattttg gtcatgagt ggaggacga gctgtagaa 
      tttgatgag ccgtactct cttttgcag aaaacggac 
      ggagtgttt tacggattg gtgcagcaa acggggaaa 
      ggaatggcc gagaatttg atgaatatt gcaaacaag 
      aatagagaa tacaccaca gaaacggat tcataaatc 
      aattaaacc caattggac aaacttgtt tttgttaat 
      aaattattg tgatcgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY