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Gene information
General information
TC013749 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4840965..4845392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013749
    iBeetle screen iB_05489

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013749 RA
      MEDNERKNL KALTKVHPK EKANFLSTL FFCWALPTF 
      VKGLKKDLD DDDLCLLKA HESKALADR MGLVWQKEK 
      NKHEIPSLG KVIIKAFYR EIIFYACFL IIQELVLKM 
      AQPLLVGKL LEYYAPNQL NVTKDMAYV YAFTLILFI 
      FSNILIQHW CYLGLNHLA MKMQIACRS LIYRKALTL 
      NKNALMKST VGQMVNLMS SDVHTFGYI CLHMHQMIL 
      APIQAVIVL YLLYSTVNG AAMVGVGLL MAIVPIQLY 
      MGKLMSFYR RRTAQKTDN RIRLMNEII CGIKIIKMF 
      TWEKPFSKL VEMARRLEL HEIKSIAYL RAVFRSVNA 
      CLTPLSIFL CVLTYILSG NTLQAQYVF VVTSFYGTI 
      RQTLTLHFP RCIAFLAEI DVSLGRIQN FLLAEETQK 
      MSSELKTDD VRVVLSEAS FKWTDFSDN GLSDVSFSV 
      NGGELVAVI GRVGSGKST LLQSILREI DLSKGELVV 
      SGSVSYAAQ EPWIFSSSI RQNILFGEK MNFERYKEV 
      VKVCALEKD FNLFPYGDR TIVGEKGVM LSGGQKARV 
      SLARAIYKD ADIYLLDDP LSAVDTHVG KQLFDQCIL 
      GFLKDKARV LVTHQIQYL GKVDEIYLL DRGQVTLRG 
      TYDELKNHK NFAKLLAEV EQTPHEDCV QEKHSVAIA 
      DTSELPTEV KEQRSSGTV SKKVYSHYF LAGNSRIFP 
      SFVLLTFVV TQIASSCVD YFLTFWVNL EQKRLEDTN 
      TEFYTNNTL LYTYVFLIL SFTFMVLVN SVCFVKFSI 
      NTCKKLHEK MFAQILNAT MRFFNTNPS GRVLNRFSK 
      DTSLVDESV PSCLTDTIN IALNVVAIT LVISSVNTW 
      IIIPTVVIF GLFYGYKII YLATSRNLK RIEGTARSP 
      MFSHLTASL QGLATIRAF NAENVLQQE FDNIQNHHS 
      AALYMYIAC SRTFSFWLD VNCIIYVAI VILSFLFIG 
      TEKYGGNVG LAITQSIAL TGMLQRGIR QWSDLENQM 
      TSVERIYEY TQLPSEPDH GTKIAPKGW PSAGNIDFN 
      DVSMKYSLD GPYVLKNLN CRIASSEKI GIVGQTGAG 
      KSSLISALF RLAFAEGKI TIDGVETSE IPLNQLRSA 
      ISIIPQEAV LFYGTLRKN LDPFDKFSD EELWNALDQ 
      VELKPTILK LAAGLSSAV SEEGSNFSV GEKQLLCMA 
      RAILHRNRI LILDEATAN VDLQTDELI QKTIRRKFR 
      DCTVLTIAH RLFTVIDSD KILVLDNGS IVEMDHPHL 
      LLQNTDGVF YNLVKQTGR AMAENLTKM AHENYMKRN 
      SLKKIML
    CDS sequence
    • >TC013749 RA
      atggaggac aacgaacgc aaaaatttg aaagccctc 
      acaaaagtg cacccaaaa gaaaaagca aactttctt 
      tctactctg ttcttctgt tgggctctc ccgaccttc 
      gtcaagggt ttgaaaaag gacctcgac gatgatgac 
      ctttgcctc ctcaaagcg cacgagtcc aaagcctta 
      gccgacaga atgggcctc gtttggcaa aaggaaaaa 
      aacaaacac gaaatccct tcattggga aaagtgata 
      atcaaagcg ttttatcga gaaattatc ttctacgcc 
      tgtttcctg ataatacag gagctagtc ctcaagatg 
      gcgcaacct ttactcgtt ggaaaactc ctggaatat 
      tacgccccc aatcagcta aacgttacg aaggacatg 
      gcttacgtg tacgctttt acgctgatt ttgttcatc 
      ttctccaat attcttatc caacattgg tgctatttg 
      ggactgaat cacctcgcc atgaagatg caaattgct 
      tgccgatcg ctcatttat cggaaagct ttgacgctg 
      aacaaaaac gctttgatg aaaagcact gtgggccag 
      atggttaat ctaatgtcc agtgacgtg catactttt 
      ggttacatc tgtttacac atgcaccaa atgattcta 
      gcaccgatt caagctgtg attgttctg tatctgctc 
      tactcgacc gtcaacgga gcagcgatg gtcggtgtt 
      ggactgctc atggctatt gtccccatc caactttat 
      atgggcaaa ctgatgtcg ttttatcgc cgcagaacg 
      gcccaaaag accgataac cgaattcgc ctaatgaac 
      gagataatc tgcggcata aaaataata aagatgttc 
      acttgggaa aaaccgttt tcgaaactg gttgaaatg 
      gctcgaaga ttggagctt cacgaaatt aaatcaatc 
      gcttatctc cgagcggtt tttcgctct gttaatgcg 
      tgtctcaca cctctgtca attttttta tgcgttttg 
      acctacatt ttgagcggt aacaccctc caagcgcag 
      tatgtgttt gttgtaact tcgttttat ggaacgata 
      cgccagact ttaacgtta catttcccc cggtgtatt 
      gctttcttg gcggaaatc gacgtttcc ctcggacga 
      atacaaaac tttctatta gctgaagag acacaaaaa 
      atgagtagt gaattgaag acagatgat gtgagggtt 
      gttttatct gaagctagt tttaaatgg acagacttt 
      tcggataat ggtttgtcc gatgtatca ttttctgta 
      aatggtggc gaattggtg gctgtaatt ggtcgtgtc 
      ggaagtggc aaaagcacc cttttgcaa tcgattctt 
      cgagaaatt gatttatct aaaggagag ttggttgtg 
      agcggatcg gtttcttac gctgcgcaa gaaccgtgg 
      attttctcg tccagtatc aggcaaaac atactgttt 
      ggcgaaaaa atgaatttt gaaaggtac aaggaagtg 
      gtcaaggtt tgcgctttg gaaaaagac ttcaacttg 
      tttccttac ggagacagg actattgta ggtgaaaag 
      ggggtgatg ttgagcggg ggtcagaaa gctcgagta 
      agtctagcg cgggctatc tacaaagac gctgatatc 
      tatttgtta gatgatcct ctatcggca gtcgacaca 
      cacgtggga aagcaactc ttcgatcag tgtattttg 
      ggttttttg aaagacaaa gcccgggtt ttggtaaca 
      caccaaatc caatacctt ggtaaagtt gatgaaatt 
      tatttgctt gaccgggga caagtcact ctaaggggt 
      acttatgac gagcttaag aatcacaaa aactttgct 
      aaattgctc gccgaagtc gagcaaaca ccgcacgaa 
      gattgcgtc caagaaaaa cactcagtt gcgatcgca 
      gatacgtcc gaattaccc actgaggta aaggaacag 
      cgcagttcg ggcacagtt tcgaaaaaa gtttattca 
      cattatttt ctcgcagga aacagtcgc attttcccg 
      tcttttgta ttactcacg tttgttgta acacagatt 
      gcatcgagc tgtgttgat tatttcctc actttttgg 
      gtgaatctt gagcaaaaa cggctcgag gacaccaat 
      actgagttt tatacaaac aacaccctt ctgtacacg 
      tatgtgttc ctaattctt tctttcacg ttcatggtt 
      ttagtgaac tcagtctgt ttcgttaaa ttctcaata 
      aacacttgc aagaaattg cacgagaaa atgtttgcc 
      caaatatta aatgccacg atgaggttt ttcaatacg 
      aatccttca ggacgtgtc cttaatcga ttttcaaaa 
      gacacgagt ctggttgat gaaagtgta ccatcgtgt 
      ctgacagac actataaat attgcttta aacgtagtt 
      gctataact cttgtgatt tcatcggta aatacatgg 
      attataata ccaacagtt gttattttc ggactattc 
      tacggttac aaaatcata tatctggcc actagccga 
      aatttgaaa cgaatcgaa gggactgcc cgaagtccc 
      atgttttca cacctgacg gcttcacta caaggacta 
      gcaacaatt cgagctttt aacgctgaa aacgtgtta 
      caacaagag ttcgacaac atccagaac caccacagc 
      gcggctttg tacatgtac atagcttgt agccgaact 
      ttttccttt tggttggac gtaaactgt attatttat 
      gtcgcaatc gtaattttg agtttccta tttattgga 
      acagaaaaa tacggtgga aatgttggt cttgccatc 
      acacagtca atcgctttg actggaatg ttgcaaaga 
      gggatccga cagtggagc gacttagaa aaccagatg 
      acttcagtc gaacgcatc tacgagtac actcaattg 
      ccctctgag ccagatcac ggcacaaaa atcgcgcct 
      aaaggctgg ccctcggcc ggaaacatc gattttaac 
      gacgtttca atgaaatat tctctcgat ggtccttac 
      gttctcaaa aatttgaac tgtcggatc gcttcaagc 
      gaaaaaatt gggattgta ggtcaaaca ggcgccggg 
      aagtcgtcc ctaatctcg gcgttgttt cgcctagcc 
      ttcgccgaa ggcaaaatc acaatcgac ggtgtcgaa 
      accagcgaa atccctcta aatcaattg cgatcagca 
      atctctata atcccccaa gaagctgtg ctgttttac 
      ggcactttg cgtaaaaat ctagacccg tttgataaa 
      ttcagcgac gaagaactg tggaatgct ttggatcaa 
      gttgaacta aaaccgact attttgaaa ttagctgcc 
      gggctttcg agcgctgtt tcggaagaa gggtcaaat 
      tttagcgtc ggagagaaa cagctcttg tgtatggcc 
      agagcgatt ctccaccga aacagaatt ttgatttta 
      gacgaagct acggccaat gttgatttg caaaccgat 
      gagttgatc cagaaaact ataaggagg aagtttcgg 
      gattgtacg gttttgacg atcgctcat cggttgttt 
      actgtcatt gattcggac aaaattctt gttttggat 
      aatgggagt attgtagag atggaccat ccccattta 
      cttttgcaa aataccgac ggagttttt tacaatctg 
      gtcaaacag acgggaagg gctatggcg gaaaatttg 
      accaaaatg gcccatgaa aactacatg aaacgcaat 
      tctttaaag aaaattatg ctataa
    mRNA sequence
    • >TC013749 RA
      tgcgaactg ttgcaaata ataatattt agtagtgat 
      tgaaatgga ggacaacga acgcaaaaa tttgaaagc 
      cctcacaaa agtgcaccc aaaagaaaa agcaaactt 
      tctttctac tctgttctt ctgttgggc tctcccgac 
      cttcgtcaa gggtttgaa aaaggacct cgacgatga 
      tgacctttg cctcctcaa agcgcacga gtccaaagc 
      cttagccga cagaatggg cctcgtttg gcaaaagga 
      aaaaaacaa acacgaaat cccttcatt gggaaaagt 
      gataatcaa agcgtttta tcgagaaat tatcttcta 
      cgcctgttt cctgataat acaggagct agtcctcaa 
      gatggcgca acctttact cgttggaaa actcctgga 
      atattacgc ccccaatca gctaaacgt tacgaagga 
      catggctta cgtgtacgc ttttacgct gattttgtt 
      catcttctc caatattct tatccaaca ttggtgcta 
      tttgggact gaatcacct cgccatgaa gatgcaaat 
      tgcttgccg atcgctcat ttatcggaa agctttgac 
      gctgaacaa aaacgcttt gatgaaaag cactgtggg 
      ccagatggt taatctaat gtccagtga cgtgcatac 
      ttttggtta catctgttt acacatgca ccaaatgat 
      tctagcacc gattcaagc tgtgattgt tctgtatct 
      gctctactc gaccgtcaa cggagcagc gatggtcgg 
      tgttggact gctcatggc tattgtccc catccaact 
      ttatatggg caaactgat gtcgtttta tcgccgcag 
      aacggccca aaagaccga taaccgaat tcgcctaat 
      gaacgagat aatctgcgg cataaaaat aataaagat 
      gttcacttg ggaaaaacc gttttcgaa actggttga 
      aatggctcg aagattgga gcttcacga aattaaatc 
      aatcgctta tctccgagc ggtttttcg ctctgttaa 
      tgcgtgtct cacacctct gtcaatttt tttatgcgt 
      tttgaccta cattttgag cggtaacac cctccaagc 
      gcagtatgt gtttgttgt aacttcgtt ttatggaac 
      gatacgcca gactttaac gttacattt cccccggtg 
      tattgcttt cttggcgga aatcgacgt ttccctcgg 
      acgaataca aaactttct attagctga agagacaca 
      aaaaatgag tagtgaatt gaagacaga tgatgtgag 
      ggttgtttt atctgaagc tagttttaa atggacaga 
      cttttcgga taatggttt gtccgatgt atcattttc 
      tgtaaatgg tggcgaatt ggtggctgt aattggtcg 
      tgtcggaag tggcaaaag caccctttt gcaatcgat 
      tcttcgaga aattgattt atctaaagg agagttggt 
      tgtgagcgg atcggtttc ttacgctgc gcaagaacc 
      gtggatttt ctcgtccag tatcaggca aaacatact 
      gtttggcga aaaaatgaa ttttgaaag gtacaagga 
      agtggtcaa ggtttgcgc tttggaaaa agacttcaa 
      cttgtttcc ttacggaga caggactat tgtaggtga 
      aaagggggt gatgttgag cgggggtca gaaagctcg 
      agtaagtct agcgcgggc tatctacaa agacgctga 
      tatctattt gttagatga tcctctatc ggcagtcga 
      cacacacgt gggaaagca actcttcga tcagtgtat 
      tttgggttt tttgaaaga caaagcccg ggttttggt 
      aacacacca aatccaata ccttggtaa agttgatga 
      aatttattt gcttgaccg gggacaagt cactctaag 
      gggtactta tgacgagct taagaatca caaaaactt 
      tgctaaatt gctcgccga agtcgagca aacaccgca 
      cgaagattg cgtccaaga aaaacactc agttgcgat 
      cgcagatac gtccgaatt acccactga ggtaaagga 
      acagcgcag ttcgggcac agtttcgaa aaaagttta 
      ttcacatta ttttctcgc aggaaacag tcgcatttt 
      cccgtcttt tgtattact cacgtttgt tgtaacaca 
      gattgcatc gagctgtgt tgattattt cctcacttt 
      ttgggtgaa tcttgagca aaaacggct cgaggacac 
      caatactga gttttatac aaacaacac ccttctgta 
      cacgtatgt gttcctaat tctttcttt cacgttcat 
      ggttttagt gaactcagt ctgtttcgt taaattctc 
      aataaacac ttgcaagaa attgcacga gaaaatgtt 
      tgcccaaat attaaatgc cacgatgag gtttttcaa 
      tacgaatcc ttcaggacg tgtccttaa tcgattttc 
      aaaagacac gagtctggt tgatgaaag tgtaccatc 
      gtgtctgac agacactat aaatattgc tttaaacgt 
      agttgctat aactcttgt gatttcatc ggtaaatac 
      atggattat aataccaac agttgttat tttcggact 
      attctacgg ttacaaaat catatatct ggccactag 
      ccgaaattt gaaacgaat cgaagggac tgcccgaag 
      tcccatgtt ttcacacct gacggcttc actacaagg 
      actagcaac aattcgagc ttttaacgc tgaaaacgt 
      gttacaaca agagttcga caacatcca gaaccacca 
      cagcgcggc tttgtacat gtacatagc ttgtagccg 
      aactttttc cttttggtt ggacgtaaa ctgtattat 
      ttatgtcgc aatcgtaat tttgagttt cctatttat 
      tggaacaga aaaatacgg tggaaatgt tggtcttgc 
      catcacaca gtcaatcgc tttgactgg aatgttgca 
      aagagggat ccgacagtg gagcgactt agaaaacca 
      gatgacttc agtcgaacg catctacga gtacactca 
      attgccctc tgagccaga tcacggcac aaaaatcgc 
      gcctaaagg ctggccctc ggccggaaa catcgattt 
      taacgacgt ttcaatgaa atattctct cgatggtcc 
      ttacgttct caaaaattt gaactgtcg gatcgcttc 
      aagcgaaaa aattgggat tgtaggtca aacaggcgc 
      cgggaagtc gtccctaat ctcggcgtt gtttcgcct 
      agccttcgc cgaaggcaa aatcacaat cgacggtgt 
      cgaaaccag cgaaatccc tctaaatca attgcgatc 
      agcaatctc tataatccc ccaagaagc tgtgctgtt 
      ttacggcac tttgcgtaa aaatctaga cccgtttga 
      taaattcag cgacgaaga actgtggaa tgctttgga 
      tcaagttga actaaaacc gactatttt gaaattagc 
      tgccgggct ttcgagcgc tgtttcgga agaagggtc 
      aaattttag cgtcggaga gaaacagct cttgtgtat 
      ggccagagc gattctcca ccgaaacag aattttgat 
      tttagacga agctacggc caatgttga tttgcaaac 
      cgatgagtt gatccagaa aactataag gaggaagtt 
      tcgggattg tacggtttt gacgatcgc tcatcggtt 
      gtttactgt cattgattc ggacaaaat tcttgtttt 
      ggataatgg gagtattgt agagatgga ccatcccca 
      tttactttt gcaaaatac cgacggagt tttttacaa 
      tctggtcaa acagacggg aagggctat ggcggaaaa 
      tttgaccaa aatggccca tgaaaacta catgaaacg 
      caattcttt aaagaaaat tatgctata attaggtgc 
      aacaatatt tagttcaat agagcaaaa ttctatgca 
      ttctgtcat tttttatca aagatttag gtaaacaag 
      atgcagcac acatattat gtttgaagt gtgagtact 
      tgcgtgggt actgatatt attgttatt gtaacattt 
      gaacaatat tgaataaat ggtaatgaa aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY