Status
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Gene information
General information
TC013745 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4886183..4889386
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013745
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013745 RA
      MVLASKALV SLTMAFAYL PVFYKLQIT SSYEYLNLR 
      FNSTVRLLG SILFLTKMM LYIPIVIYV PALAFSQVT 
      GINLHLVTP VVCIVCIFY TTLGGLKAV VWTDTIQTI 
      MMFAALIGV VALGVASVG GFAEVWRRN EEGDRIDFF 
      NFDWDPTIR HTFWTVAIG NYFSWLASC SINQAMVQR 
      CLAMPTLKS ARITVAILV VGIMALVSM CCFMGLIIY 
      AYYYKCDPI TRGLVHKSD QLLPFFVMH IASSMPGLP 
      GLFVSGVFS AALSSMSTG LNSMTGVIF EDLIKPRIK 
      KPLSEARAS FLMKVIVVI IGSICVALV FVVEKMGAL 
      IQATGSLSA ITAGPLLGI FSLGMFFPN ANPQGALIG 
      GLVSGSLIG WISVGTQMN MAQGVIRFP QKYVSVEGC 
      NADWVAEYL SLTLSKDTK EIPVEAPFV LYRLSYMYY 
      TLVGAVTTI VVGFIVSVL TGGAQHVNR DLLSPVIYP 
      FLKRHKVEK QQNDVIKEI SL
    • >TC013745 RB
      MSMAAIYIS GISILGIPA EIYTYGTQF WMVLASKAL 
      VSLTMAFAY LPVFYKLQI TSSYEYLNL RFNSTVRLL 
      GSILFLTKM MLYIPIVIY VPALAFSQV TGINLHLVT 
      PVVCIVCIF YTTLGGLKA VVWTDTIQT IMMFAALIG 
      VVALGVASV GGFAEVWRR NEEGDRIDF FNFDWDPTI 
      RHTFWTVAI GNYFSWLAS CSINQAMVQ RCLAMPTLK 
      SARITVAIL VVGIMALVS MCCFMGLII YAYYYKCDP 
      ITRGLVHKS DQLLPFFVM HIASSMPGL PGLFVSGVF 
      SAALSSMST GLNSMTGVI FEDLIKPRI KKPLSEARA 
      SFLMKVIVV IIGSICVAL VFVVEKMGA LIQATGSLS 
      AITAGPLLG IFSLGMFFP NANPQGALI GGLVSGSLI 
      GWISVGTQM NMAQGVIRF PQKYVSVEG CNADWVAEY 
      LSLTLSKDT KEIPVEAPF VLYRLSYMY YTLVGAVTT 
      IVVGFIVSV LTGGAQHVN RDLLSPVIY PFLKRHKVE 
      KQQNDVIKE ISL
    CDS sequence
    • >TC013745 RA
      atggttttg gcgtccaaa gcgctagtt tccctaacg 
      atggctttt gcctatttg cctgtcttt tacaaattg 
      caaataact tcaagctac gagtattta aatctccgc 
      ttcaactca accgtgcgc ttactcggc tccattctc 
      ttccttaca aaaatgatg ctctacatc cctatagtg 
      atctacgtc ccagcctta gctttcagc caagtcact 
      ggaattaat ctacatttg gtgacgccg gttgtgtgt 
      attgtttgc attttttac acaactttg gggggtttg 
      aaagccgtg gtttggact gataccatc cagactata 
      atgatgttc gctgctttg ataggtgtt gtagcactt 
      ggagtcgca agtgttgga ggctttgca gaagtatgg 
      cggcggaac gaggaaggg gacaggatc gattttttc 
      aattttgac tgggacccc acaatcaga cacacattt 
      tggacagtt gcgatcgga aattatttc tcatggttg 
      gcttcttgt tccataaac caggcaatg gttcaaagg 
      tgtctggca atgcccaca ttgaaatct gctcgaata 
      actgtggcc atactggtg gtcggcata atggcccta 
      gtttcaatg tgctgtttc atgggcttg ataatttac 
      gcttattac tacaaatgc gatcccata actagaggc 
      ctagttcac aagtccgac caattactg ccatttttt 
      gtaatgcat attgcgtca tcgatgccc ggacttccc 
      ggacttttc gttagcggc gttttcagt gctgcctta 
      agctccatg tccactggg ctaaactcg atgacgggc 
      gtgatattc gaagactta atcaagccc cggatcaag 
      aagccgctg tctgaagcc cgggctagt tttctgatg 
      aaagtcatt gttgtgata attgggagt atttgcgtg 
      gctcttgtc ttcgttgtg gaaaaaatg ggggccctt 
      atccaggcc actggcagc ctcagtgcc atcactgcc 
      ggccccctt ttggggatt ttctccctg gggatgttc 
      ttccccaac gccaacccc cagggggcg cttattggg 
      ggtttggtc agcggaagc ctgatcggg tggatttcc 
      gtcggtact cagatgaat atggctcag ggggttatc 
      agatttccg cagaagtat gtctcggtt gagggctgc 
      aacgccgat tgggtcgcg gagtatttg agtttgacg 
      ctgtccaaa gacacgaag gaaattcca gtggaagcc 
      ccctttgtg ctatatagg ctgtcctac atgtattac 
      actctagtg ggggctgtt actacgatc gtggtgggt 
      tttatcgtg agtgtgtta acaggaggt gcccaacat 
      gttaaccga gatttgcta tcgccggtg atttacccg 
      tttttgaaa cgacacaaa gtggaaaag caacaaaac 
      gatgtaatt aaagaaatt tcgctataa
    • >TC013745 RB
      atgagtatg gcagcaatc tacatttct ggtataagc 
      atactggga atccccgcc gagatctac acttatggg 
      acgcaattt tggatggtt ttggcgtcc aaagcgcta 
      gtttcccta acgatggct tttgcctat ttgcctgtc 
      ttttacaaa ttgcaaata acttcaagc tacgagtat 
      ttaaatctc cgcttcaac tcaaccgtg cgcttactc 
      ggctccatt ctcttcctt acaaaaatg atgctctac 
      atccctata gtgatctac gtcccagcc ttagctttc 
      agccaagtc actggaatt aatctacat ttggtgacg 
      ccggttgtg tgtattgtt tgcattttt tacacaact 
      ttggggggt ttgaaagcc gtggtttgg actgatacc 
      atccagact ataatgatg ttcgctgct ttgataggt 
      gttgtagca cttggagtc gcaagtgtt ggaggcttt 
      gcagaagta tggcggcgg aacgaggaa ggggacagg 
      atcgatttt ttcaatttt gactgggac cccacaatc 
      agacacaca ttttggaca gttgcgatc ggaaattat 
      ttctcatgg ttggcttct tgttccata aaccaggca 
      atggttcaa aggtgtctg gcaatgccc acattgaaa 
      tctgctcga ataactgtg gccatactg gtggtcggc 
      ataatggcc ctagtttca atgtgctgt ttcatgggc 
      ttgataatt tacgcttat tactacaaa tgcgatccc 
      ataactaga ggcctagtt cacaagtcc gaccaatta 
      ctgccattt tttgtaatg catattgcg tcatcgatg 
      cccggactt cccggactt ttcgttagc ggcgttttc 
      agtgctgcc ttaagctcc atgtccact gggctaaac 
      tcgatgacg ggcgtgata ttcgaagac ttaatcaag 
      ccccggatc aagaagccg ctgtctgaa gcccgggct 
      agttttctg atgaaagtc attgttgtg ataattggg 
      agtatttgc gtggctctt gtcttcgtt gtggaaaaa 
      atgggggcc cttatccag gccactggc agcctcagt 
      gccatcact gccggcccc cttttgggg attttctcc 
      ctggggatg ttcttcccc aacgccaac ccccagggg 
      gcgcttatt gggggtttg gtcagcgga agcctgatc 
      gggtggatt tccgtcggt actcagatg aatatggct 
      cagggggtt atcagattt ccgcagaag tatgtctcg 
      gttgagggc tgcaacgcc gattgggtc gcggagtat 
      ttgagtttg acgctgtcc aaagacacg aaggaaatt 
      ccagtggaa gcccccttt gtgctatat aggctgtcc 
      tacatgtat tacactcta gtgggggct gttactacg 
      atcgtggtg ggttttatc gtgagtgtg ttaacagga 
      ggtgcccaa catgttaac cgagatttg ctatcgccg 
      gtgatttac ccgtttttg aaacgacac aaagtggaa 
      aagcaacaa aacgatgta attaaagaa atttcgcta 
      taa
    mRNA sequence
    • >TC013745 RA
      acaaaattc aaactgcaa gacgattta tttgtaatt 
      cggctattt taaggagtt ctgagaagt ctttgggca 
      aatatgtaa atatgtatg caacaatga cttgtttgt 
      ctttcatgt cctgaaact tgggattgg ttactcacc 
      ctaaaaact ggactaact gtaccaaga aaaagccga 
      caagaaatg aaaaatata gttcaaaat tccattcgt 
      tttgtataa ctcaacaca cacacttct aacacacta 
      cagcctttg ggtatctgt cgttacatt gttggtacc 
      actggcaaa atttcttaa tatgactac atttctggt 
      ataagcata ctgggaatc cccgccgag atctacact 
      tatgggacg caattttgg atggttttg gcgtccaaa 
      gcgctagtt tccctaacg atggctttt gcctatttg 
      cctgtcttt tacaaattg caaataact tcaagctac 
      gagtattta aatctccgc ttcaactca accgtgcgc 
      ttactcggc tccattctc ttccttaca aaaatgatg 
      ctctacatc cctatagtg atctacgtc ccagcctta 
      gctttcagc caagtcact ggaattaat ctacatttg 
      gtgacgccg gttgtgtgt attgtttgc attttttac 
      acaactttg gggggtttg aaagccgtg gtttggact 
      gataccatc cagactata atgatgttc gctgctttg 
      ataggtgtt gtagcactt ggagtcgca agtgttgga 
      ggctttgca gaagtatgg cggcggaac gaggaaggg 
      gacaggatc gattttttc aattttgac tgggacccc 
      acaatcaga cacacattt tggacagtt gcgatcgga 
      aattatttc tcatggttg gcttcttgt tccataaac 
      caggcaatg gttcaaagg tgtctggca atgcccaca 
      ttgaaatct gctcgaata actgtggcc atactggtg 
      gtcggcata atggcccta gtttcaatg tgctgtttc 
      atgggcttg ataatttac gcttattac tacaaatgc 
      gatcccata actagaggc ctagttcac aagtccgac 
      caattactg ccatttttt gtaatgcat attgcgtca 
      tcgatgccc ggacttccc ggacttttc gttagcggc 
      gttttcagt gctgcctta agctccatg tccactggg 
      ctaaactcg atgacgggc gtgatattc gaagactta 
      atcaagccc cggatcaag aagccgctg tctgaagcc 
      cgggctagt tttctgatg aaagtcatt gttgtgata 
      attgggagt atttgcgtg gctcttgtc ttcgttgtg 
      gaaaaaatg ggggccctt atccaggcc actggcagc 
      ctcagtgcc atcactgcc ggccccctt ttggggatt 
      ttctccctg gggatgttc ttccccaac gccaacccc 
      cagggggcg cttattggg ggtttggtc agcggaagc 
      ctgatcggg tggatttcc gtcggtact cagatgaat 
      atggctcag ggggttatc agatttccg cagaagtat 
      gtctcggtt gagggctgc aacgccgat tgggtcgcg 
      gagtatttg agtttgacg ctgtccaaa gacacgaag 
      gaaattcca gtggaagcc ccctttgtg ctatatagg 
      ctgtcctac atgtattac actctagtg ggggctgtt 
      actacgatc gtggtgggt tttatcgtg agtgtgtta 
      acaggaggt gcccaacat gttaaccga gatttgcta 
      tcgccggtg atttacccg tttttgaaa cgacacaaa 
      gtggaaaag caacaaaac gatgtaatt aaagaaatt 
      tcgctataa atgtatgta taatataat caaaaataa 
      atagtagcc acacttact ctacctaat acacttaac 
      atagcaat
    • >TC013745 RB
      acaaaattc aaactgcaa gacgattta tttgtaatt 
      cggctattt taaggagtt ctgagaagt ctttgggca 
      aatatgtaa atatgtatg caacaatga cttgtttgt 
      ctttcatgt cctgaaact tgggattgg ttactcacc 
      ctaaaaact ggactaact gtaccaaga aaaagccga 
      caagaaatg aaaaatata gttcaaaat tccattcgt 
      tttgtataa ctcaacaca cacacttct aacacacta 
      cagcctttg ggtatctgt cgttacatt gttggtacc 
      actggcaaa atttcttaa tatgagtat ggcagcaat 
      ctacatttc tggtataag catactggg aatccccgc 
      cgagatcta cacttatgg gacgcaatt ttggatggt 
      tttggcgtc caaagcgct agtttccct aacgatggc 
      ttttgccta tttgcctgt cttttacaa attgcaaat 
      aacttcaag ctacgagta tttaaatct ccgcttcaa 
      ctcaaccgt gcgcttact cggctccat tctcttcct 
      tacaaaaat gatgctcta catccctat agtgatcta 
      cgtcccagc cttagcttt cagccaagt cactggaat 
      taatctaca tttggtgac gccggttgt gtgtattgt 
      ttgcatttt ttacacaac tttgggggg tttgaaagc 
      cgtggtttg gactgatac catccagac tataatgat 
      gttcgctgc tttgatagg tgttgtagc acttggagt 
      cgcaagtgt tggaggctt tgcagaagt atggcggcg 
      gaacgagga aggggacag gatcgattt tttcaattt 
      tgactggga ccccacaat cagacacac attttggac 
      agttgcgat cggaaatta tttctcatg gttggcttc 
      ttgttccat aaaccaggc aatggttca aaggtgtct 
      ggcaatgcc cacattgaa atctgctcg aataactgt 
      ggccatact ggtggtcgg cataatggc cctagtttc 
      aatgtgctg tttcatggg cttgataat ttacgctta 
      ttactacaa atgcgatcc cataactag aggcctagt 
      tcacaagtc cgaccaatt actgccatt ttttgtaat 
      gcatattgc gtcatcgat gcccggact tcccggact 
      tttcgttag cggcgtttt cagtgctgc cttaagctc 
      catgtccac tgggctaaa ctcgatgac gggcgtgat 
      attcgaaga cttaatcaa gccccggat caagaagcc 
      gctgtctga agcccgggc tagttttct gatgaaagt 
      cattgttgt gataattgg gagtatttg cgtggctct 
      tgtcttcgt tgtggaaaa aatgggggc ccttatcca 
      ggccactgg cagcctcag tgccatcac tgccggccc 
      ccttttggg gattttctc cctggggat gttcttccc 
      caacgccaa cccccaggg ggcgcttat tgggggttt 
      ggtcagcgg aagcctgat cgggtggat ttccgtcgg 
      tactcagat gaatatggc tcagggggt tatcagatt 
      tccgcagaa gtatgtctc ggttgaggg ctgcaacgc 
      cgattgggt cgcggagta tttgagttt gacgctgtc 
      caaagacac gaaggaaat tccagtgga agccccctt 
      tgtgctata taggctgtc ctacatgta ttacactct 
      agtgggggc tgttactac gatcgtggt gggttttat 
      cgtgagtgt gttaacagg aggtgccca acatgttaa 
      ccgagattt gctatcgcc ggtgattta cccgttttt 
      gaaacgaca caaagtgga aaagcaaca aaacgatgt 
      aattaaaga aatttcgct ataaatgta tgtataata 
      taatcaaaa ataaatagt agccacact tactctacc 
      taatacact taacatagc aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY