| General information | |||
| TC013741 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 4959963..4966526 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013741 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05486 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC013741 RA
MGLKKFIVV FVALATIST SHAFIHLTP HDKEVVYTW KALVKAGTD LPTSHASQF VLEGKIHLQ SNPNATHVR ITDLTYKLY NGILDHVRE HEAHSVPVQ IHDLEHAFR VVYDTNGLA IGIATNGQE KEFSRNIKK ALATILQMD GSVVKNIGH HHQHPHSFV TREHSIYGR FDVDYTVRS LENGRVEVH KLHDMHDSR HLYQHLFSN NEAGHCDAH YENPIMHDS RKDYLIVIQ DGHHIVKHV QAVGALVYH PFKAQSDHQ FILVNQSLH LHHVGPIHQ QLHVESEHF EDHLTYHPF EDQQDGRLH DLTNGRHVV NYDKVIPDV QHMLEDIHQ YLHEDHIHT REPDSKRGQ LINRVQRLL QHFNSAKLE ELHKKLTGE AARIFHHIL PLVGTRASV LYIKHLVVD KVVHEDVAV DLLQHFPEH VVEPTIELL TQLETLIHL DENVHWDVR RAALLSFAH LVHHSHIHQ HTHDDAHTH DNQHIHDNR HTHDDEQPY EKYVVEYIN KLRSAHDYR TQMVYAFGL SNMKLHSII KHMLPAVNG EWYNDHHFR LLSLWAMGS AVVRDHDPS TVIETLWPI FTNVDETTE MRCLAFFHI MESRPSDPR IANMYSYLI NEPNEEVYR FVHSYMHTV SHSHDPCKE DFWVRMAHL VNFLPPPDH GTSSVHFHD YRDINFGFS GGVQEYFIN SNESQIYGV ATTTEHFNI HHEGYSFYL KIHRIRHRG APIPLLFIE TFMKKLRDI NNNDLHIEL VVFRHGRVI NTHFFDEQN WHELDAILD FFTKDSKSL MKDLLHVSY DEYHRALIP TDLGIPAVW EYLMPVVHK NNISVTKET AKKVINIHV DNRYVNWIH YRHGLSFYN PIVDVWQGI NRFHSYDAV WPLHFDISI NTQQQSLQL SWKRHNNAQ LDVAGLRSH VKQMVFIKD DHHRNVLGQ SSPQCKEFV IVGHGKQFR HDYPLLHDD DLSTGLQWH VAVYDSDRY VTNGSFQHL LHLFTQSHH RHSETPLSH VLTSWANWH QYVLLLPRS GTYGFITRI EPSHQHPVS NIDLSLRLH SEPASEETH YYAPRIKWS SRVTYAVKN HDTSLKTWD VNAIFDINP GHTVNNLKM IVTRIVPGQ NDYKICVDG VKKWTDKGV TGHLNVAMS QTPDGKCVK DDTVIDVTM TGEKSHEQQ EGHHKYGTC EYISPYSVP HWHTLECLV DHTTVRRYV YNVKTTNVP SEFKKVFAH WWDHLKGYY VSYYHLEDE HNDHIGDHN LRLDIHYPM EEETVNVHV TTPQHVYSL TGVPTHHWR WVGLSPDSV EYSKSFQYL HDSGWVDHC VVHVDHHRL NQHDVHETV PNDWTLFVG NGAQNPTMG VYIKRVAND EIAVRVVDN GHTLEIAPH GITHQITVD GRVVPVQEY HHEDWAYWH VFNQDYHHP VAVLLHHSG LHLEYHHHE VVVVVPKLD HLPHGHCYR HH
- >TC013741 RA
atgggactg aagaagttc attgtggtg tttgtcgct ttggcgaca atttccaca tcacacgcc ttcatccac ttgacgcct cacgacaaa gaggtggtt tacacctgg aaggcactc gtcaaagct ggcaccgac ttacccacc tcacacgca tctcagttc gttctcgaa ggaaaaatt cacctgcag tccaatcct aacgcgacc catgtccga atcacagac ctgacgtac aaactctac aatggtatt ctggaccat gtgcgcgag cacgaggcc catagcgtt ccagtgcaa atacatgac ctggagcac gccttcaga gtggtttat gacaccaat ggtttggcg attggaatc gcaaccaat gggcaagag aaggaattc tcgcgcaat atcaagaaa gcgttagct accatttta cagatggat gggtccgtg gtgaaaaac attggacac caccaccaa cacccgcat tcgtttgta acaagggaa cattcgatt tatggccgg tttgatgtt gattacacg gtcagaagc ctcgagaat gggagggtg gaggttcac aaactgcac gatatgcat gattcgagg catttgtac caacacttg tttagtaat aatgaagct gggcattgc gatgcccat tacgaaaac cccatcatg catgatagc cgcaaagac tatttgata gttatacaa gatgggcac catatcgtc aaacatgtc caggctgtt ggtgctttg gtttatcac ccatttaaa gctcagtcc gaccaccag tttattctg gtcaatcaa agccttcac ctccatcac gttggaccc attcatcag caacttcac gttgaaagt gaacacttt gaagaccat ttgacttat cacccgttc gaggatcaa caagatggc cgcttgcat gacttgacc aatggccgc catgttgtt aactatgac aaggtcatt cctgacgtc caacacatg ttggaggac atccaccag tacctccac gaagaccac atacacacc cgggagccg gactccaaa cgtggtcaa ttgataaac cgagtccaa agactcctc caacacttt aactcggcc aaactcgaa gaactccac aagaaattg accggtgaa gctgcacgc atcttccac cacattcta ccactagta gggacacga gcgtccgtt ctgtacatc aagcacttg gtagttgat aaagtggtc catgaggac gtcgcggtt gacctcctc caacacttc ccagaacac gtggtggaa cccacaatt gaactactg acacaactt gaaaccctg atacatctg gacgaaaac gtccattgg gacgtccgc agggccgct cttctgagc ttcgcccac ctcgtccac cactcccac atccaccag cacacccac gatgacgcg catacccat gataaccaa cacatccat gacaataga cacacccat gacgatgaa caaccctac gagaagtac gtggtggag tacataaac aagctgagg tcggcacac gactaccgc acccaaatg gtttacgcc tttggcctc tccaacatg aagctccac agcatcatc aagcacatg ctgcctgca gtcaacggc gagtggtac aacgaccac cacttccgc ctacttagt ctctgggcc atgggttcg gccgtggta cgtgaccac gacccgagc accgtcatc gaaaccctc tggcccatt ttcaccaac gttgacgaa accaccgaa atgagatgt ctggccttt ttccacatc atggaaagt agacccagc gacccaaga atcgcaaac atgtactca tatttgatc aatgaaccc aacgaagaa gtctaccgt ttcgtgcac tcgtacatg cacaccgtc agccactcg catgaccct tgcaaggag gacttctgg gtcaggatg gcccacctt gtcaacttc ctaccaccg cccgaccac ggaacgtcc tccgtccat ttccacgac tacagagac atcaatttc gggttcagc ggcggcgtc caggagtat ttcattaat tcgaacgaa tcgcaaatt tacggcgtt gccacgacc accgaacat ttcaatatt caccacgaa ggctacagt ttctacttg aaaattcac aggatacga catcgcgga gccccgatt cctcttctc ttcatcgaa acgtttatg aaaaaattg agagatatc aacaacaat gacctccac atcgaattg gttgtgttc aggcacgga cgggtcatc aacacccac ttctttgac gaacaaaac tggcacgag ttggacgcg attttggac tttttcacc aaagactcg aaatcgctg atgaaagac ctgctccat gtgagttac gatgagtac cacagagcg ttgattccc actgatctg ggcattccg gccgtttgg gaatacttg atgcccgtc gtccacaaa aacaacatc agtgtcacg aaggagaca gccaagaaa gttataaat atccatgtt gataacagg tacgtcaac tggattcac tatcgccac gggttgtct ttctacaac ccgattgtc gatgtgtgg caaggcatc aaccgattc cactcgtac gatgccgtc tggcccctg catttcgac atttcgatc aacacgcag cagcaaagt ttgcagttg tcgtggaag aggcataac aatgcccaa ctggacgtg gcgggactc agaagccat gtcaaacaa atggtgttt attaaagac gaccaccac cgcaatgtt ttagggcag tccagtccg cagtgtaaa gagttcgtc atcgtgggc catggcaag caattcaga catgattac ccgctactc cacgacgat gatttgagc actggcctc caatggcat gtcgcagtc tacgacagt gatcgctac gtcacgaac ggttccttc caacactta ctccacctc ttcacacaa tcccaccac cgccactct gaaaccccc ctaagccat gttctaaca agttgggcc aattggcac caatacgtc ctgttgttg ccccgatct ggcacctat ggttttatc acaagaatt gagccaagt caccaacat ccagtcagt aacattgac ctgtccctt cgcctacat tccgagcca gccagtgaa gaaacgcat tattacgcc ccgagaatc aaatggagc tctcgagtg acctacgcc gtgaaaaac cacgacact agtctcaaa acctgggac gtgaacgca atcttcgac ataaaccca ggccacaca gtcaataat ctcaaaatg atcgtcact aggatcgtc ccggggcaa aatgactac aaaatttgc gtggatggc gtcaagaaa tggaccgat aagggcgtg accgggcat ttgaacgtg gccatgagc caaaccccc gatggcaaa tgcgtgaaa gacgacacc gtgattgac gtcaccatg accggggaa aagtcacac gaacagcag gaaggccac cacaaatat ggcacttgc gagtacatc agcccctac tccgtccca cactggcac actttggag tgcctggtc gaccatacc accgtcaga cgctacgtt tacaacgtc aaaaccact aatgtccca tccgagttc aagaaagtg ttcgcccat tggtgggac cacctcaaa ggctactac gtgtcgtac taccacctc gaagacgag cacaatgac cacatcggc gaccataac ctccgcctg gacatccac taccccatg gaggaggaa acagtcaat gtgcacgtc acgacccca caacacgtc tatagcctg actggggtg cccacccac cactggcga tgggtgggc ttgagccca gattcggtc gaatactcc aaaagcttc caatacttg cacgattcc ggctgggtt gaccattgc gtggttcat gtcgaccac caccgactc aaccaacac gacgtccat gaaacggta ccaaatgac tggactttg ttcgtcgga aacggtgcc caaaaccca acaatggga gtctacatc aaacgtgtt gccaatgat gaaattgcg gttcgtgtt gtcgacaat gggcacaca cttgaaatt gctcctcat ggaatcacc catcagatt actgtagat gggcgcgtg gtcccggtc caggagtac caccacgag gattgggct tattggcac gtgtttaac caagactac catcatcct gtcgccgtt ttgctgcac cacagtgga cttcacctg gagtaccac caccatgaa gtggttgtg gtggtgccc aaactggac catctgccg cacggccac tgctataga catcactag
- >TC013741 RA
tggttgctt cgaccatgg gactgaaga agttcattg tggtgtttg tcgctttgg cgacaattt ccacatcac acgccttca tccacttga cgcctcacg acaaagagg tggtttaca cctggaagg cactcgtca aagctggca ccgacttac ccacctcac acgcatctc agttcgttc tcgaaggaa aaattcacc tgcagtcca atcctaacg cgacccatg tccgaatca cagacctga cgtacaaac tctacaatg gtattctgg accatgtgc gcgagcacg aggcccata gcgttccag tgcaaatac atgacctgg agcacgcct tcagagtgg tttatgaca ccaatggtt tggcgattg gaatcgcaa ccaatgggc aagagaagg aattctcgc gcaatatca agaaagcgt tagctacca ttttacaga tggatgggt ccgtggtga aaaacattg gacaccacc accaacacc cgcattcgt ttgtaacaa gggaacatt cgatttatg gccggtttg atgttgatt acacggtca gaagcctcg agaatggga gggtggagg ttcacaaac tgcacgata tgcatgatt cgaggcatt tgtaccaac acttgttta gtaataatg aagctgggc attgcgatg cccattacg aaaacccca tcatgcatg atagccgca aagactatt tgatagtta tacaagatg ggcaccata tcgtcaaac atgtccagg ctgttggtg ctttggttt atcacccat ttaaagctc agtccgacc accagttta ttctggtca atcaaagcc ttcacctcc atcacgttg gacccattc atcagcaac ttcacgttg aaagtgaac actttgaag accatttga cttatcacc cgttcgagg atcaacaag atggccgct tgcatgact tgaccaatg gccgccatg ttgttaact atgacaagg tcattcctg acgtccaac acatgttgg aggacatcc accagtacc tccacgaag accacatac acacccggg agccggact ccaaacgtg gtcaattga taaaccgag tccaaagac tcctccaac actttaact cggccaaac tcgaagaac tccacaaga aattgaccg gtgaagctg cacgcatct tccaccaca ttctaccac tagtaggga cacgagcgt ccgttctgt acatcaagc acttggtag ttgataaag tggtccatg aggacgtcg cggttgacc tcctccaac acttcccag aacacgtgg tggaaccca caattgaac tactgacac aacttgaaa ccctgatac atctggacg aaaacgtcc attgggacg tccgcaggg ccgctcttc tgagcttcg cccacctcg tccaccact cccacatcc accagcaca cccacgatg acgcgcata cccatgata accaacaca tccatgaca atagacaca cccatgacg atgaacaac cctacgaga agtacgtgg tggagtaca taaacaagc tgaggtcgg cacacgact accgcaccc aaatggttt acgcctttg gcctctcca acatgaagc tccacagca tcatcaagc acatgctgc ctgcagtca acggcgagt ggtacaacg accaccact tccgcctac ttagtctct gggccatgg gttcggccg tggtacgtg accacgacc cgagcaccg tcatcgaaa ccctctggc ccattttca ccaacgttg acgaaacca ccgaaatga gatgtctgg cctttttcc acatcatgg aaagtagac ccagcgacc caagaatcg caaacatgt actcatatt tgatcaatg aacccaacg aagaagtct accgtttcg tgcactcgt acatgcaca ccgtcagcc actcgcatg acccttgca aggaggact tctgggtca ggatggccc accttgtca acttcctac caccgcccg accacggaa cgtcctccg tccatttcc acgactaca gagacatca atttcgggt tcagcggcg gcgtccagg agtatttca ttaattcga acgaatcgc aaatttacg gcgttgcca cgaccaccg aacatttca atattcacc acgaaggct acagtttct acttgaaaa ttcacagga tacgacatc gcggagccc cgattcctc ttctcttca tcgaaacgt ttatgaaaa aattgagag atatcaaca acaatgacc tccacatcg aattggttg tgttcaggc acggacggg tcatcaaca cccacttct ttgacgaac aaaactggc acgagttgg acgcgattt tggactttt tcaccaaag actcgaaat cgctgatga aagacctgc tccatgtga gttacgatg agtaccaca gagcgttga ttcccactg atctgggca ttccggccg tttgggaat acttgatgc ccgtcgtcc acaaaaaca acatcagtg tcacgaagg agacagcca agaaagtta taaatatcc atgttgata acaggtacg tcaactgga ttcactatc gccacgggt tgtctttct acaacccga ttgtcgatg tgtggcaag gcatcaacc gattccact cgtacgatg ccgtctggc ccctgcatt tcgacattt cgatcaaca cgcagcagc aaagtttgc agttgtcgt ggaagaggc ataacaatg cccaactgg acgtggcgg gactcagaa gccatgtca aacaaatgg tgtttatta aagacgacc accaccgca atgttttag ggcagtcca gtccgcagt gtaaagagt tcgtcatcg tgggccatg gcaagcaat tcagacatg attacccgc tactccacg acgatgatt tgagcactg gcctccaat ggcatgtcg cagtctacg acagtgatc gctacgtca cgaacggtt ccttccaac acttactcc acctcttca cacaatccc accaccgcc actctgaaa cccccctaa gccatgttc taacaagtt gggccaatt ggcaccaat acgtcctgt tgttgcccc gatctggca cctatggtt ttatcacaa gaattgagc caagtcacc aacatccag tcagtaaca ttgacctgt cccttcgcc tacattccg agccagcca gtgaagaaa cgcattatt acgccccga gaatcaaat ggagctctc gagtgacct acgccgtga aaaaccacg acactagtc tcaaaacct gggacgtga acgcaatct tcgacataa acccaggcc acacagtca ataatctca aaatgatcg tcactagga tcgtcccgg ggcaaaatg actacaaaa tttgcgtgg atggcgtca agaaatgga ccgataagg gcgtgaccg ggcatttga acgtggcca tgagccaaa cccccgatg gcaaatgcg tgaaagacg acaccgtga ttgacgtca ccatgaccg gggaaaagt cacacgaac agcaggaag gccaccaca aatatggca cttgcgagt acatcagcc cctactccg tcccacact ggcacactt tggagtgcc tggtcgacc ataccaccg tcagacgct acgtttaca acgtcaaaa ccactaatg tcccatccg agttcaaga aagtgttcg cccattggt gggaccacc tcaaaggct actacgtgt cgtactacc acctcgaag acgagcaca atgaccaca tcggcgacc ataacctcc gcctggaca tccactacc ccatggagg aggaaacag tcaatgtgc acgtcacga ccccacaac acgtctata gcctgactg gggtgccca cccaccact ggcgatggg tgggcttga gcccagatt cggtcgaat actccaaaa gcttccaat acttgcacg attccggct gggttgacc attgcgtgg ttcatgtcg accaccacc gactcaacc aacacgacg tccatgaaa cggtaccaa atgactgga ctttgttcg tcggaaacg gtgcccaaa acccaacaa tgggagtct acatcaaac gtgttgcca atgatgaaa ttgcggttc gtgttgtcg acaatgggc acacacttg aaattgctc ctcatggaa tcacccatc agattactg tagatgggc gcgtggtcc cggtccagg agtaccacc acgaggatt gggcttatt ggcacgtgt ttaaccaag actaccatc atcctgtcg ccgttttgc tgcaccaca gtggacttc acctggagt accaccacc atgaagtgg ttgtggtgg tgcccaaac tggaccatc tgccgcacg gccactgct atagacatc actaggttg ctgtatacg aatttggca cgtattatg gtattgtag tcttataca tataataaa gcaaggttt ttaaa
