Status
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Gene information
General information
TC013741 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4959963..4966526
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013741
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05486

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013741 RA
      MGLKKFIVV FVALATIST SHAFIHLTP HDKEVVYTW 
      KALVKAGTD LPTSHASQF VLEGKIHLQ SNPNATHVR 
      ITDLTYKLY NGILDHVRE HEAHSVPVQ IHDLEHAFR 
      VVYDTNGLA IGIATNGQE KEFSRNIKK ALATILQMD 
      GSVVKNIGH HHQHPHSFV TREHSIYGR FDVDYTVRS 
      LENGRVEVH KLHDMHDSR HLYQHLFSN NEAGHCDAH 
      YENPIMHDS RKDYLIVIQ DGHHIVKHV QAVGALVYH 
      PFKAQSDHQ FILVNQSLH LHHVGPIHQ QLHVESEHF 
      EDHLTYHPF EDQQDGRLH DLTNGRHVV NYDKVIPDV 
      QHMLEDIHQ YLHEDHIHT REPDSKRGQ LINRVQRLL 
      QHFNSAKLE ELHKKLTGE AARIFHHIL PLVGTRASV 
      LYIKHLVVD KVVHEDVAV DLLQHFPEH VVEPTIELL 
      TQLETLIHL DENVHWDVR RAALLSFAH LVHHSHIHQ 
      HTHDDAHTH DNQHIHDNR HTHDDEQPY EKYVVEYIN 
      KLRSAHDYR TQMVYAFGL SNMKLHSII KHMLPAVNG 
      EWYNDHHFR LLSLWAMGS AVVRDHDPS TVIETLWPI 
      FTNVDETTE MRCLAFFHI MESRPSDPR IANMYSYLI 
      NEPNEEVYR FVHSYMHTV SHSHDPCKE DFWVRMAHL 
      VNFLPPPDH GTSSVHFHD YRDINFGFS GGVQEYFIN 
      SNESQIYGV ATTTEHFNI HHEGYSFYL KIHRIRHRG 
      APIPLLFIE TFMKKLRDI NNNDLHIEL VVFRHGRVI 
      NTHFFDEQN WHELDAILD FFTKDSKSL MKDLLHVSY 
      DEYHRALIP TDLGIPAVW EYLMPVVHK NNISVTKET 
      AKKVINIHV DNRYVNWIH YRHGLSFYN PIVDVWQGI 
      NRFHSYDAV WPLHFDISI NTQQQSLQL SWKRHNNAQ 
      LDVAGLRSH VKQMVFIKD DHHRNVLGQ SSPQCKEFV 
      IVGHGKQFR HDYPLLHDD DLSTGLQWH VAVYDSDRY 
      VTNGSFQHL LHLFTQSHH RHSETPLSH VLTSWANWH 
      QYVLLLPRS GTYGFITRI EPSHQHPVS NIDLSLRLH 
      SEPASEETH YYAPRIKWS SRVTYAVKN HDTSLKTWD 
      VNAIFDINP GHTVNNLKM IVTRIVPGQ NDYKICVDG 
      VKKWTDKGV TGHLNVAMS QTPDGKCVK DDTVIDVTM 
      TGEKSHEQQ EGHHKYGTC EYISPYSVP HWHTLECLV 
      DHTTVRRYV YNVKTTNVP SEFKKVFAH WWDHLKGYY 
      VSYYHLEDE HNDHIGDHN LRLDIHYPM EEETVNVHV 
      TTPQHVYSL TGVPTHHWR WVGLSPDSV EYSKSFQYL 
      HDSGWVDHC VVHVDHHRL NQHDVHETV PNDWTLFVG 
      NGAQNPTMG VYIKRVAND EIAVRVVDN GHTLEIAPH 
      GITHQITVD GRVVPVQEY HHEDWAYWH VFNQDYHHP 
      VAVLLHHSG LHLEYHHHE VVVVVPKLD HLPHGHCYR 
      HH
    CDS sequence
    • >TC013741 RA
      atgggactg aagaagttc attgtggtg tttgtcgct 
      ttggcgaca atttccaca tcacacgcc ttcatccac 
      ttgacgcct cacgacaaa gaggtggtt tacacctgg 
      aaggcactc gtcaaagct ggcaccgac ttacccacc 
      tcacacgca tctcagttc gttctcgaa ggaaaaatt 
      cacctgcag tccaatcct aacgcgacc catgtccga 
      atcacagac ctgacgtac aaactctac aatggtatt 
      ctggaccat gtgcgcgag cacgaggcc catagcgtt 
      ccagtgcaa atacatgac ctggagcac gccttcaga 
      gtggtttat gacaccaat ggtttggcg attggaatc 
      gcaaccaat gggcaagag aaggaattc tcgcgcaat 
      atcaagaaa gcgttagct accatttta cagatggat 
      gggtccgtg gtgaaaaac attggacac caccaccaa 
      cacccgcat tcgtttgta acaagggaa cattcgatt 
      tatggccgg tttgatgtt gattacacg gtcagaagc 
      ctcgagaat gggagggtg gaggttcac aaactgcac 
      gatatgcat gattcgagg catttgtac caacacttg 
      tttagtaat aatgaagct gggcattgc gatgcccat 
      tacgaaaac cccatcatg catgatagc cgcaaagac 
      tatttgata gttatacaa gatgggcac catatcgtc 
      aaacatgtc caggctgtt ggtgctttg gtttatcac 
      ccatttaaa gctcagtcc gaccaccag tttattctg 
      gtcaatcaa agccttcac ctccatcac gttggaccc 
      attcatcag caacttcac gttgaaagt gaacacttt 
      gaagaccat ttgacttat cacccgttc gaggatcaa 
      caagatggc cgcttgcat gacttgacc aatggccgc 
      catgttgtt aactatgac aaggtcatt cctgacgtc 
      caacacatg ttggaggac atccaccag tacctccac 
      gaagaccac atacacacc cgggagccg gactccaaa 
      cgtggtcaa ttgataaac cgagtccaa agactcctc 
      caacacttt aactcggcc aaactcgaa gaactccac 
      aagaaattg accggtgaa gctgcacgc atcttccac 
      cacattcta ccactagta gggacacga gcgtccgtt 
      ctgtacatc aagcacttg gtagttgat aaagtggtc 
      catgaggac gtcgcggtt gacctcctc caacacttc 
      ccagaacac gtggtggaa cccacaatt gaactactg 
      acacaactt gaaaccctg atacatctg gacgaaaac 
      gtccattgg gacgtccgc agggccgct cttctgagc 
      ttcgcccac ctcgtccac cactcccac atccaccag 
      cacacccac gatgacgcg catacccat gataaccaa 
      cacatccat gacaataga cacacccat gacgatgaa 
      caaccctac gagaagtac gtggtggag tacataaac 
      aagctgagg tcggcacac gactaccgc acccaaatg 
      gtttacgcc tttggcctc tccaacatg aagctccac 
      agcatcatc aagcacatg ctgcctgca gtcaacggc 
      gagtggtac aacgaccac cacttccgc ctacttagt 
      ctctgggcc atgggttcg gccgtggta cgtgaccac 
      gacccgagc accgtcatc gaaaccctc tggcccatt 
      ttcaccaac gttgacgaa accaccgaa atgagatgt 
      ctggccttt ttccacatc atggaaagt agacccagc 
      gacccaaga atcgcaaac atgtactca tatttgatc 
      aatgaaccc aacgaagaa gtctaccgt ttcgtgcac 
      tcgtacatg cacaccgtc agccactcg catgaccct 
      tgcaaggag gacttctgg gtcaggatg gcccacctt 
      gtcaacttc ctaccaccg cccgaccac ggaacgtcc 
      tccgtccat ttccacgac tacagagac atcaatttc 
      gggttcagc ggcggcgtc caggagtat ttcattaat 
      tcgaacgaa tcgcaaatt tacggcgtt gccacgacc 
      accgaacat ttcaatatt caccacgaa ggctacagt 
      ttctacttg aaaattcac aggatacga catcgcgga 
      gccccgatt cctcttctc ttcatcgaa acgtttatg 
      aaaaaattg agagatatc aacaacaat gacctccac 
      atcgaattg gttgtgttc aggcacgga cgggtcatc 
      aacacccac ttctttgac gaacaaaac tggcacgag 
      ttggacgcg attttggac tttttcacc aaagactcg 
      aaatcgctg atgaaagac ctgctccat gtgagttac 
      gatgagtac cacagagcg ttgattccc actgatctg 
      ggcattccg gccgtttgg gaatacttg atgcccgtc 
      gtccacaaa aacaacatc agtgtcacg aaggagaca 
      gccaagaaa gttataaat atccatgtt gataacagg 
      tacgtcaac tggattcac tatcgccac gggttgtct 
      ttctacaac ccgattgtc gatgtgtgg caaggcatc 
      aaccgattc cactcgtac gatgccgtc tggcccctg 
      catttcgac atttcgatc aacacgcag cagcaaagt 
      ttgcagttg tcgtggaag aggcataac aatgcccaa 
      ctggacgtg gcgggactc agaagccat gtcaaacaa 
      atggtgttt attaaagac gaccaccac cgcaatgtt 
      ttagggcag tccagtccg cagtgtaaa gagttcgtc 
      atcgtgggc catggcaag caattcaga catgattac 
      ccgctactc cacgacgat gatttgagc actggcctc 
      caatggcat gtcgcagtc tacgacagt gatcgctac 
      gtcacgaac ggttccttc caacactta ctccacctc 
      ttcacacaa tcccaccac cgccactct gaaaccccc 
      ctaagccat gttctaaca agttgggcc aattggcac 
      caatacgtc ctgttgttg ccccgatct ggcacctat 
      ggttttatc acaagaatt gagccaagt caccaacat 
      ccagtcagt aacattgac ctgtccctt cgcctacat 
      tccgagcca gccagtgaa gaaacgcat tattacgcc 
      ccgagaatc aaatggagc tctcgagtg acctacgcc 
      gtgaaaaac cacgacact agtctcaaa acctgggac 
      gtgaacgca atcttcgac ataaaccca ggccacaca 
      gtcaataat ctcaaaatg atcgtcact aggatcgtc 
      ccggggcaa aatgactac aaaatttgc gtggatggc 
      gtcaagaaa tggaccgat aagggcgtg accgggcat 
      ttgaacgtg gccatgagc caaaccccc gatggcaaa 
      tgcgtgaaa gacgacacc gtgattgac gtcaccatg 
      accggggaa aagtcacac gaacagcag gaaggccac 
      cacaaatat ggcacttgc gagtacatc agcccctac 
      tccgtccca cactggcac actttggag tgcctggtc 
      gaccatacc accgtcaga cgctacgtt tacaacgtc 
      aaaaccact aatgtccca tccgagttc aagaaagtg 
      ttcgcccat tggtgggac cacctcaaa ggctactac 
      gtgtcgtac taccacctc gaagacgag cacaatgac 
      cacatcggc gaccataac ctccgcctg gacatccac 
      taccccatg gaggaggaa acagtcaat gtgcacgtc 
      acgacccca caacacgtc tatagcctg actggggtg 
      cccacccac cactggcga tgggtgggc ttgagccca 
      gattcggtc gaatactcc aaaagcttc caatacttg 
      cacgattcc ggctgggtt gaccattgc gtggttcat 
      gtcgaccac caccgactc aaccaacac gacgtccat 
      gaaacggta ccaaatgac tggactttg ttcgtcgga 
      aacggtgcc caaaaccca acaatggga gtctacatc 
      aaacgtgtt gccaatgat gaaattgcg gttcgtgtt 
      gtcgacaat gggcacaca cttgaaatt gctcctcat 
      ggaatcacc catcagatt actgtagat gggcgcgtg 
      gtcccggtc caggagtac caccacgag gattgggct 
      tattggcac gtgtttaac caagactac catcatcct 
      gtcgccgtt ttgctgcac cacagtgga cttcacctg 
      gagtaccac caccatgaa gtggttgtg gtggtgccc 
      aaactggac catctgccg cacggccac tgctataga 
      catcactag
    mRNA sequence
    • >TC013741 RA
      tggttgctt cgaccatgg gactgaaga agttcattg 
      tggtgtttg tcgctttgg cgacaattt ccacatcac 
      acgccttca tccacttga cgcctcacg acaaagagg 
      tggtttaca cctggaagg cactcgtca aagctggca 
      ccgacttac ccacctcac acgcatctc agttcgttc 
      tcgaaggaa aaattcacc tgcagtcca atcctaacg 
      cgacccatg tccgaatca cagacctga cgtacaaac 
      tctacaatg gtattctgg accatgtgc gcgagcacg 
      aggcccata gcgttccag tgcaaatac atgacctgg 
      agcacgcct tcagagtgg tttatgaca ccaatggtt 
      tggcgattg gaatcgcaa ccaatgggc aagagaagg 
      aattctcgc gcaatatca agaaagcgt tagctacca 
      ttttacaga tggatgggt ccgtggtga aaaacattg 
      gacaccacc accaacacc cgcattcgt ttgtaacaa 
      gggaacatt cgatttatg gccggtttg atgttgatt 
      acacggtca gaagcctcg agaatggga gggtggagg 
      ttcacaaac tgcacgata tgcatgatt cgaggcatt 
      tgtaccaac acttgttta gtaataatg aagctgggc 
      attgcgatg cccattacg aaaacccca tcatgcatg 
      atagccgca aagactatt tgatagtta tacaagatg 
      ggcaccata tcgtcaaac atgtccagg ctgttggtg 
      ctttggttt atcacccat ttaaagctc agtccgacc 
      accagttta ttctggtca atcaaagcc ttcacctcc 
      atcacgttg gacccattc atcagcaac ttcacgttg 
      aaagtgaac actttgaag accatttga cttatcacc 
      cgttcgagg atcaacaag atggccgct tgcatgact 
      tgaccaatg gccgccatg ttgttaact atgacaagg 
      tcattcctg acgtccaac acatgttgg aggacatcc 
      accagtacc tccacgaag accacatac acacccggg 
      agccggact ccaaacgtg gtcaattga taaaccgag 
      tccaaagac tcctccaac actttaact cggccaaac 
      tcgaagaac tccacaaga aattgaccg gtgaagctg 
      cacgcatct tccaccaca ttctaccac tagtaggga 
      cacgagcgt ccgttctgt acatcaagc acttggtag 
      ttgataaag tggtccatg aggacgtcg cggttgacc 
      tcctccaac acttcccag aacacgtgg tggaaccca 
      caattgaac tactgacac aacttgaaa ccctgatac 
      atctggacg aaaacgtcc attgggacg tccgcaggg 
      ccgctcttc tgagcttcg cccacctcg tccaccact 
      cccacatcc accagcaca cccacgatg acgcgcata 
      cccatgata accaacaca tccatgaca atagacaca 
      cccatgacg atgaacaac cctacgaga agtacgtgg 
      tggagtaca taaacaagc tgaggtcgg cacacgact 
      accgcaccc aaatggttt acgcctttg gcctctcca 
      acatgaagc tccacagca tcatcaagc acatgctgc 
      ctgcagtca acggcgagt ggtacaacg accaccact 
      tccgcctac ttagtctct gggccatgg gttcggccg 
      tggtacgtg accacgacc cgagcaccg tcatcgaaa 
      ccctctggc ccattttca ccaacgttg acgaaacca 
      ccgaaatga gatgtctgg cctttttcc acatcatgg 
      aaagtagac ccagcgacc caagaatcg caaacatgt 
      actcatatt tgatcaatg aacccaacg aagaagtct 
      accgtttcg tgcactcgt acatgcaca ccgtcagcc 
      actcgcatg acccttgca aggaggact tctgggtca 
      ggatggccc accttgtca acttcctac caccgcccg 
      accacggaa cgtcctccg tccatttcc acgactaca 
      gagacatca atttcgggt tcagcggcg gcgtccagg 
      agtatttca ttaattcga acgaatcgc aaatttacg 
      gcgttgcca cgaccaccg aacatttca atattcacc 
      acgaaggct acagtttct acttgaaaa ttcacagga 
      tacgacatc gcggagccc cgattcctc ttctcttca 
      tcgaaacgt ttatgaaaa aattgagag atatcaaca 
      acaatgacc tccacatcg aattggttg tgttcaggc 
      acggacggg tcatcaaca cccacttct ttgacgaac 
      aaaactggc acgagttgg acgcgattt tggactttt 
      tcaccaaag actcgaaat cgctgatga aagacctgc 
      tccatgtga gttacgatg agtaccaca gagcgttga 
      ttcccactg atctgggca ttccggccg tttgggaat 
      acttgatgc ccgtcgtcc acaaaaaca acatcagtg 
      tcacgaagg agacagcca agaaagtta taaatatcc 
      atgttgata acaggtacg tcaactgga ttcactatc 
      gccacgggt tgtctttct acaacccga ttgtcgatg 
      tgtggcaag gcatcaacc gattccact cgtacgatg 
      ccgtctggc ccctgcatt tcgacattt cgatcaaca 
      cgcagcagc aaagtttgc agttgtcgt ggaagaggc 
      ataacaatg cccaactgg acgtggcgg gactcagaa 
      gccatgtca aacaaatgg tgtttatta aagacgacc 
      accaccgca atgttttag ggcagtcca gtccgcagt 
      gtaaagagt tcgtcatcg tgggccatg gcaagcaat 
      tcagacatg attacccgc tactccacg acgatgatt 
      tgagcactg gcctccaat ggcatgtcg cagtctacg 
      acagtgatc gctacgtca cgaacggtt ccttccaac 
      acttactcc acctcttca cacaatccc accaccgcc 
      actctgaaa cccccctaa gccatgttc taacaagtt 
      gggccaatt ggcaccaat acgtcctgt tgttgcccc 
      gatctggca cctatggtt ttatcacaa gaattgagc 
      caagtcacc aacatccag tcagtaaca ttgacctgt 
      cccttcgcc tacattccg agccagcca gtgaagaaa 
      cgcattatt acgccccga gaatcaaat ggagctctc 
      gagtgacct acgccgtga aaaaccacg acactagtc 
      tcaaaacct gggacgtga acgcaatct tcgacataa 
      acccaggcc acacagtca ataatctca aaatgatcg 
      tcactagga tcgtcccgg ggcaaaatg actacaaaa 
      tttgcgtgg atggcgtca agaaatgga ccgataagg 
      gcgtgaccg ggcatttga acgtggcca tgagccaaa 
      cccccgatg gcaaatgcg tgaaagacg acaccgtga 
      ttgacgtca ccatgaccg gggaaaagt cacacgaac 
      agcaggaag gccaccaca aatatggca cttgcgagt 
      acatcagcc cctactccg tcccacact ggcacactt 
      tggagtgcc tggtcgacc ataccaccg tcagacgct 
      acgtttaca acgtcaaaa ccactaatg tcccatccg 
      agttcaaga aagtgttcg cccattggt gggaccacc 
      tcaaaggct actacgtgt cgtactacc acctcgaag 
      acgagcaca atgaccaca tcggcgacc ataacctcc 
      gcctggaca tccactacc ccatggagg aggaaacag 
      tcaatgtgc acgtcacga ccccacaac acgtctata 
      gcctgactg gggtgccca cccaccact ggcgatggg 
      tgggcttga gcccagatt cggtcgaat actccaaaa 
      gcttccaat acttgcacg attccggct gggttgacc 
      attgcgtgg ttcatgtcg accaccacc gactcaacc 
      aacacgacg tccatgaaa cggtaccaa atgactgga 
      ctttgttcg tcggaaacg gtgcccaaa acccaacaa 
      tgggagtct acatcaaac gtgttgcca atgatgaaa 
      ttgcggttc gtgttgtcg acaatgggc acacacttg 
      aaattgctc ctcatggaa tcacccatc agattactg 
      tagatgggc gcgtggtcc cggtccagg agtaccacc 
      acgaggatt gggcttatt ggcacgtgt ttaaccaag 
      actaccatc atcctgtcg ccgttttgc tgcaccaca 
      gtggacttc acctggagt accaccacc atgaagtgg 
      ttgtggtgg tgcccaaac tggaccatc tgccgcacg 
      gccactgct atagacatc actaggttg ctgtatacg 
      aatttggca cgtattatg gtattgtag tcttataca 
      tataataaa gcaaggttt ttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY