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Gene information
General information
TC013717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5422186..5424948
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013717
    iBeetle screen iB_05482

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013717 RA
      MMLKAYDGQ KDKSDNEDS YYIHNNSYY TSSPPSYKE 
      VNGNISESS PIKNLAKEQ LLNSINANT NGTNTTTII 
      AEIEGEVKK KTKPIDKTY YIAKEILMT ELTYKKDLD 
      VINVWFRDE VGKEEPDEC SILLTLIAP LAQAHGHLV 
      KDLEQRLQG WDGRGGPRA TTSQKIADV LLTHLPPLL 
      PIYEEYLDG HILVLERLD AAFKQNSHF EQLYRDFET 
      QKVCYLPLT TFVLKPLHR LLQYQSLLQ RLLRYYGPS 
      HPDRADCLT ASDTLKDLM QPVCETLEH SENLATLCE 
      LQRDLVGFD NLIQPGRKF IRHGCLLKH SKKGYQQRM 
      FFLFSDILL YTSRSQATL QFKVHGHMP LRGVLIEEP 
      EGELATYGL VIYGGNRAL TVAASSQEE KERWKQDLQ 
      NAIQEARDK TDTKITYLS LKSCSSSDE IMDQCGNDV 
      GTQTKPQPQ RSNLKVHVC WHRNTSISM KDELVAVEN 
      QLSGYLLRK FKSSNGWQK LWVVLTSFC LFFYKGCMD 
      DFPLASLPL LGYTVGAPA PEDAIGKDY VFKLQYKNH 
      VYFFRAESE YTYNRWMEV IGSCTQSRK KKQIIVNEN 
      SIECSPEK
    CDS sequence
    • >TC013717 RA
      atgatgctc aaagcatat gatggtcag aaggacaag 
      tcggataac gaagactct tattacatc cacaacaac 
      tcctactac accagctcc cctccgagt tacaaggaa 
      gtaaacggc aacatttcg gaatccagc ccgataaaa 
      aatctcgca aaagagcag cttctgaac agtattaac 
      gccaacacg aacggcacc aacaccaca acgataatc 
      gccgagatt gaaggcgaa gtgaagaag aaaactaaa 
      ccgatcgac aaaacctac tacatcgcc aaagaaatc 
      ctcatgact gaactcaca tacaagaaa gacttagat 
      gtgattaat gtgtggttt cgggacgaa gtcggtaaa 
      gaagaaccg gacgaatgc tcaattttg ctcacctta 
      atcgcgcct ttggcacaa gctcacgga cacctagtc 
      aaggatttg gagcagcgg ttgcaaggg tgggacggc 
      aggggcggc ccgagggcc accaccagt cagaaaatc 
      gccgatgtt ttgctcaca cacttaccc cctttgctt 
      ccaatttat gaagagtac ttggacggc cacattttg 
      gtgttggag cgcttggac gccgcattt aagcaaaac 
      agtcatttc gagcagctg taccgtgat tttgaaacg 
      cagaaagtg tgctactta ccgctcaca actttcgta 
      ttgaagcca ctacatcgc ttgttgcaa taccagtcg 
      ttgttgcaa agactttta cgttattat gggccttca 
      caccccgat cgtgccgat tgtttaact gctagtgac 
      actttgaaa gatttaatg caaccagtt tgcgaaact 
      ttggaacat tcggaaaat ttagccact ttgtgtgaa 
      cttcaacgc gatctagtg ggcttcgat aatttgata 
      cagcccggg cggaaattt atcagacac gggtgttta 
      ctcaaacat tccaagaag ggttaccaa caacgcatg 
      ttctttttg ttctctgac attctttta tacacttct 
      cggtctcag gccactcta cagttcaaa gtccacggt 
      catatgcca ctaagaggt gtcctgatt gaggaaccg 
      gaaggagaa ttggcgact tatggctta gtgatttat 
      ggaggtaat cgtgcgttg actgtggcg gcgagttcg 
      caggaagag aaggaaagg tggaagcaa gacttgcaa 
      aatgccatt caggaagct agggataag acggacacg 
      aaaataact tatttgagc ctaaaaagt tgcagctct 
      tcggatgaa attatggac cagtgtggt aacgacgtt 
      ggcacgcaa acaaagcca caaccacag aggagtaat 
      ttgaaagtg cacgtgtgt tggcataga aatactagt 
      attagtatg aaagacgaa ttggtggca gttgagaat 
      caattatcg ggttatttg ttgcgcaaa tttaaaagt 
      agcaacggt tggcagaag ttatgggtt gttttgacg 
      tctttttgt ttattcttt tataaagga tgtatggat 
      gattttcca ttggcgtca ctgcctttg ctaggatat 
      acagttggc gctcctgcg cctgaggac gccatcgga 
      aaagactac gttttcaag ctacaatac aaaaatcat 
      gtgtatttt ttcagagcc gagtctgaa tatacttac 
      aatagatgg atggaagtg atcgggagt tgtactcag 
      agcaggaag aagaaacaa ataattgtg aacgaaaac 
      tcaatcgaa tgttcgccg gaaaagtag
    mRNA sequence
    • >TC013717 RA
      agttggaac aaatgttaa cgtttttcg tcatacttt 
      gttttgttg agatatttg ttgattatt atgatgctc 
      aaagcatat gatggtcag aaggacaag tcggataac 
      gaagactct tattacatc cacaacaac tcctactac 
      accagctcc cctccgagt tacaaggaa gtaaacggc 
      aacatttcg gaatccagc ccgataaaa aatctcgca 
      aaagagcag cttctgaac agtattaac gccaacacg 
      aacggcacc aacaccaca acgataatc gccgagatt 
      gaaggcgaa gtgaagaag aaaactaaa ccgatcgac 
      aaaacctac tacatcgcc aaagaaatc ctcatgact 
      gaactcaca tacaagaaa gacttagat gtgattaat 
      gtgtggttt cgggacgaa gtcggtaaa gaagaaccg 
      gacgaatgc tcaattttg ctcacctta atcgcgcct 
      ttggcacaa gctcacgga cacctagtc aaggatttg 
      gagcagcgg ttgcaaggg tgggacggc aggggcggc 
      ccgagggcc accaccagt cagaaaatc gccgatgtt 
      ttgctcaca cacttaccc cctttgctt ccaatttat 
      gaagagtac ttggacggc cacattttg gtgttggag 
      cgcttggac gccgcattt aagcaaaac agtcatttc 
      gagcagctg taccgtgat tttgaaacg cagaaagtg 
      tgctactta ccgctcaca actttcgta ttgaagcca 
      ctacatcgc ttgttgcaa taccagtcg ttgttgcaa 
      agactttta cgttattat gggccttca caccccgat 
      cgtgccgat tgtttaact gctagtgac actttgaaa 
      gatttaatg caaccagtt tgcgaaact ttggaacat 
      tcggaaaat ttagccact ttgtgtgaa cttcaacgc 
      gatctagtg ggcttcgat aatttgata cagcccggg 
      cggaaattt atcagacac gggtgttta ctcaaacat 
      tccaagaag ggttaccaa caacgcatg ttctttttg 
      ttctctgac attctttta tacacttct cggtctcag 
      gccactcta cagttcaaa gtccacggt catatgcca 
      ctaagaggt gtcctgatt gaggaaccg gaaggagaa 
      ttggcgact tatggctta gtgatttat ggaggtaat 
      cgtgcgttg actgtggcg gcgagttcg caggaagag 
      aaggaaagg tggaagcaa gacttgcaa aatgccatt 
      caggaagct agggataag acggacacg aaaataact 
      tatttgagc ctaaaaagt tgcagctct tcggatgaa 
      attatggac cagtgtggt aacgacgtt ggcacgcaa 
      acaaagcca caaccacag aggagtaat ttgaaagtg 
      cacgtgtgt tggcataga aatactagt attagtatg 
      aaagacgaa ttggtggca gttgagaat caattatcg 
      ggttatttg ttgcgcaaa tttaaaagt agcaacggt 
      tggcagaag ttatgggtt gttttgacg tctttttgt 
      ttattcttt tataaagga tgtatggat gattttcca 
      ttggcgtca ctgcctttg ctaggatat acagttggc 
      gctcctgcg cctgaggac gccatcgga aaagactac 
      gttttcaag ctacaatac aaaaatcat gtgtatttt 
      ttcagagcc gagtctgaa tatacttac aatagatgg 
      atggaagtg atcgggagt tgtactcag agcaggaag 
      aagaaacaa ataattgtg aacgaaaac tcaatcgaa 
      tgttcgccg gaaaagtag ttttattta tgtttcaag 
      gaatttcta tttttaggt tttaacggt tttgttagc 
      aactaatta tgtaaatat ggttaagta ttattgaaa 
      tttaaagga ctggataag tcattccta ggtaattgt 
      agtatttcc gtactcata tttgtatat atttgtaca 
      ttttttatc gattttact cctttttac atattttga 
      tgtcgctgt ctcagcagc tagtttaat tctagtgcc 
      tctaacaaa ctcattttg ttattaatt aggaagttt 
      atgttgtac attttcatt ttatgaaac atcactcgt 
      agttaattt tctggatga aatatattt gatgaaata 
      aattatttt tacaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY