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Gene information
General information
TC013702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5705561..5710180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013702
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05480

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition number 2nd lateral oviduct: 2 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013702 RA
      MKVTVTTLT DFIFVLDVS EDLELENFK AFCEVETGF 
      PATEIVIAY NGMPLMDNK KSLKEHGIR DGDAVCLQH 
      MLQHGASQS SFDLQGVPR LDFSGIQVP NSTRGNQVS 
      PGRPSEDDP VLIRDMFLA NPDQLALLK QNNPRLADA 
      LLTGNIDTF ASVLREQVQ ARQEREQQR LRMLNADPF 
      DTEAQRLIA EEIRQKNIE ANMEAAMEY NPESFGTVV 
      MLYINCRVN GYPVKAFID SGAQTTIMS SSCAERCNI 
      MRLVDTRWA GIAKGVGVQ KIIGRIHMV QIQIESDYL 
      TTSFSVLEN QPMDMLLGL DMLKRHQCC IDLRANVLR 
      IGTTGTETK FLAESDLPE CARLSTISE EDAISRSIK 
      ESEDRQLQE ALRNSQLSN PTSGSSVQG AKVTPAPSN 
      TQLLPTDKF TESDVKELT GLGFSREQV ISELRAFNG 
      DKTQATAAL FAKSLKF
    CDS sequence
    • >TC013702 RA
      atgaaagtg acagtgacc acattgact gactttatt 
      ttcgtcctc gacgtctcc gaagacttg gagttggaa 
      aatttcaaa gcattttgt gaagtggaa acagggttt 
      ccggccacg gaaatcgtc atcgcctac aatggaatg 
      cctttaatg gacaacaaa aagtcgctg aaggagcac 
      gggatccgc gacggtgat gccgtctgc ctccaacac 
      atgctgcag catggggct tcacagagt tcctttgat 
      ttgcaagga gttcctaga ctcgatttc agcgggatc 
      caagttccc aactctacc agaggtaat caggtgtct 
      ccaggacgc ccttctgaa gatgaccct gtgctaatc 
      agggacatg tttttggca aatcccgac cagttagcg 
      ttgctgaag caaaacaat ccgcggctg gctgacgcc 
      ttgctgact ggtaacata gacactttt gcttctgtt 
      ctgcgggag caagtgcaa gcgcgccaa gaacgtgag 
      caacaaaga ctaagaatg ttgaatgcc gatccgttc 
      gatacggag gctcagcgc ctcatcgct gaagaaatc 
      cgtcaaaaa aacatcgaa gcaaacatg gaggctgcc 
      atggagtac aaccccgaa tcattcggg acagtcgtt 
      atgctttac attaactgt agagttaac ggctacccc 
      gttaaagcc tttattgac tcaggtgct caaactacc 
      ataatgtcc agcagttgt gcagaaaga tgtaacata 
      atgcggctg gtggacacg agatgggca ggaatcgcg 
      aaaggcgtc ggggtgcag aaaatcatc gggagaatt 
      cacatggtg caaatccaa atcgagagc gattatttg 
      acgaccagt ttctccgta cttgaaaac caacccatg 
      gacatgctg ctggggttg gatatgctg aaaagacac 
      caatgttgc atcgatttg cgcgcaaac gtcttaaga 
      attgggaca acagggact gaaacgaaa tttcttgcc 
      gagtcggat ttgcccgag tgcgccaga ctgagtaca 
      attagtgaa gaagatgcc atttctagg tcgatcaag 
      gagtcggaa gaccggcag ctgcaggaa gctctgagg 
      aatagtcag ttatcaaat cccacttct gggagttca 
      gttcagggt gccaaagtc actccagcg ccaagcaat 
      actcagctt ttaccaaca gacaaattt accgaaagt 
      gacgttaaa gaactgaca ggtttgggt ttctcgcgg 
      gaacaagtc atatctgag ttgcgtgcg tttaacggt 
      gataaaaca caagcgact gccgcattg ttcgcaaaa 
      tctttaaaa ttttaa
    mRNA sequence
    • >TC013702 RA
      gcatgattc tgtcatatt tgtctttcc aaccgttgt 
      tattttagc gttttccat tataaatca gcaaaatga 
      aagtgacag tgaccacat tgactgact ttattttcg 
      tcctcgacg tctccgaag acttggagt tggaaaatt 
      tcaaagcat tttgtgaag tggaaacag ggtttccgg 
      ccacggaaa tcgtcatcg cctacaatg gaatgcctt 
      taatggaca acaaaaagt cgctgaagg agcacggga 
      tccgcgacg gtgatgccg tctgcctcc aacacatgc 
      tgcagcatg gggcttcac agagttcct ttgatttgc 
      aaggagttc ctagactcg atttcagcg ggatccaag 
      ttcccaact ctaccagag gtaatcagg tgtctccag 
      gacgccctt ctgaagatg accctgtgc taatcaggg 
      acatgtttt tggcaaatc ccgaccagt tagcgttgc 
      tgaagcaaa acaatccgc ggctggctg acgccttgc 
      tgactggta acatagaca cttttgctt ctgttctgc 
      gggagcaag tgcaagcgc gccaagaac gtgagcaac 
      aaagactaa gaatgttga atgccgatc cgttcgata 
      cggaggctc agcgcctca tcgctgaag aaatccgtc 
      aaaaaaaca tcgaagcaa acatggagg ctgccatgg 
      agtacaacc ccgaatcat tcgggacag tcgttatgc 
      tttacatta actgtagag ttaacggct accccgtta 
      aagccttta ttgactcag gtgctcaaa ctaccataa 
      tgtccagca gttgtgcag aaagatgta acataatgc 
      ggctggtgg acacgagat gggcaggaa tcgcgaaag 
      gcgtcgggg tgcagaaaa tcatcggga gaattcaca 
      tggtgcaaa tccaaatcg agagcgatt atttgacga 
      ccagtttct ccgtacttg aaaaccaac ccatggaca 
      tgctgctgg ggttggata tgctgaaaa gacaccaat 
      gttgcatcg atttgcgcg caaacgtct taagaattg 
      ggacaacag ggactgaaa cgaaatttc ttgccgagt 
      cggatttgc ccgagtgcg ccagactga gtacaatta 
      gtgaagaag atgccattt ctaggtcga tcaaggagt 
      cggaagacc ggcagctgc aggaagctc tgaggaata 
      gtcagttat caaatccca cttctggga gttcagttc 
      agggtgcca aagtcactc cagcgccaa gcaatactc 
      agcttttac caacagaca aatttaccg aaagtgacg 
      ttaaagaac tgacaggtt tgggtttct cgcgggaac 
      aagtcatat ctgagttgc gtgcgttta acggtgata 
      aaacacaag cgactgccg cattgttcg caaaatctt 
      taaaatttt aagtgcatt taaactggt cacacgtta 
      gcttttcta gactaggtt ttgatattg tggtcgata 
      ataactctt aaattgaca tatctacac aaaatttgg 
      gtttttatt attattggc tggcccatt gaacgtcgt 
      tttacgttt ccttataat agtgcatct taagactgt 
      atattttgc tctcaaata ttatattaa ctcaagcag 
      aaaattgca ccactccac agccttttt ggtggacag 
      ggaactgta gaagccata gctcccaag ttacgaatt 
      tttgaactt taacctaat ccgcaaatt tgtttaaaa 
      ggttttacg aattaaatg attcgtgta aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY