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Gene information
General information
TC013699 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5738157..5740593
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013699
    iBeetle screen iB_05478

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 7

    wings not closed - Number of animals showing the phenotype: 7
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 3


    hindwing irregular - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013699 RA
      MSRKRDISR EEIAKQFLL PERQSKIDT LLKQDGQAY 
      CICRSSDSS RFMIACDAC EEWYHGDCM NISEKEAKM 
      IKQYICIRC SEEDSTLQT RWRTKRDEI ASANTNTEE 
      RKARKRKER GDTKNDKKM KKCGDCMGC YRTEDCGRC 
      DVCTRRSRH GSPRNKERC KQRICINYG GGRRKRRDS 
      SSDREGPPN AHLMTDYPR QCYGPTCTN SARYGSKYC 
      SDQCGLKLA TNRILQVLP QRIQEWALS PCSAEESNI 
      KALDQVRKQ QFEVKQILQ ELDSRHREL DAIIERAKS 
      ATIDPNADF DNDEESEVS TMYCITCGL EIHSKTAIK 
      HMEKCFNKY ESQASFGSI FKTRIEGNN MFCDFYNPA 
      NRTYCKRLR VLCPEHCKD PKIGEYEVC GCPLVTNVF 
      DLTGEFCRA PKKSCVKHH VWEKLRRAE IDLERVRQW 
      LKMDELLEK ERQIRNTMA SRAGVLSLM LHSTYNHEV 
      MEQITKASQ QDQNAMQRE LAKRFRTST KPRFT
    CDS sequence
    • >TC013699 RA
      atgtctcga aaacgcgat atttcgcgc gaagagatc 
      gctaaacag tttctgtta ccggagcgt caatccaaa 
      atcgacacc ctcctcaag caagatggt caagcttat 
      tgcatttgt cgctcctcg gacagctcc cgtttcatg 
      attgcgtgt gacgcctgt gaagaatgg taccacggc 
      gattgcatg aacatctcc gaaaaggag gccaaaatg 
      atcaaacaa tacatttgt attcgctgt tccgaggag 
      gactcaact ctgcagact cgttggcgg accaaacgc 
      gacgaaatc gccagtgcc aacaccaac accgaggag 
      cgcaaagcc cgcaaacgc aaagaacga ggtgacacc 
      aaaaacgac aaaaaaatg aaaaaatgc ggggactgt 
      atggggtgt taccgcacc gaggactgc gggcggtgc 
      gacgtgtgc acgaggcgg agccgtcac ggctccccg 
      cgcaacaag gagcggtgc aagcagcgg atttgcata 
      aattacggc ggggggagg cgcaagagg cgggattca 
      agctcggac agggaaggc ccgcccaat gcccacctc 
      atgaccgac tatccgcgc caatgctac gggcccacg 
      tgtaccaat agcgcccgc tacggctcg aaatactgc 
      tcggatcag tgtggctta aagttggca accaatcgg 
      attttgcaa gtgttgcca cagcgtatc caagagtgg 
      gccctgagt ccctgctct gccgaagag tccaatatc 
      aaagctctc gaccaagtc aggaagcaa cagttcgaa 
      gtcaaacag attttgcaa gagttggac agccggcat 
      cgcgaattg gacgcgatt atcgagcgc gccaagagc 
      gccaccatt gatccgaac gccgatttc gacaacgac 
      gaagagtcg gaagtgtcg accatgtat tgcataaca 
      tgcggactc gaaatacat tcgaaaacg gccatcaaa 
      cacatggaa aagtgtttc aacaagtac gaatctcaa 
      gcgagtttc gggagtatt tttaaaacc agaattgag 
      gggaataac atgttttgc gatttttat aatccagct 
      aatcggact tattgtaaa agattgagg gttttgtgc 
      ccagagcat tgcaaggat ccgaaaatc ggcgaatat 
      gaagtctgt ggctgtcca ttggtcacc aacgttttc 
      gatctgaca ggggagttt tgtcgagcg ccgaaaaaa 
      tcatgcgtt aagcatcac gtttgggag aaattgaga 
      cgggctgag atcgacttg gaacgggtg cgccagtgg 
      ttgaaaatg gacgaactt ttagaaaaa gagcgccaa 
      attcgcaac acaatggcc tcgagagcc ggagtgctc 
      tctctgatg ctgcactcg acatacaat cacgaagtc 
      atggaacaa ataacaaaa gcgagtcag caggaccaa 
      aacgcgatg caaagagag ttggctaag agattccgc 
      acgtctacc aaaccacgt ttcacgtaa
    mRNA sequence
    • >TC013699 RA
      agctaggaa aagtggcta aaagaagca aggttaagt 
      taattgaag atgtctcga aaacgcgat atttcgcgc 
      gaagagatc gctaaacag tttctgtta ccggagcgt 
      caatccaaa atcgacacc ctcctcaag caagatggt 
      caagcttat tgcatttgt cgctcctcg gacagctcc 
      cgtttcatg attgcgtgt gacgcctgt gaagaatgg 
      taccacggc gattgcatg aacatctcc gaaaaggag 
      gccaaaatg atcaaacaa tacatttgt attcgctgt 
      tccgaggag gactcaact ctgcagact cgttggcgg 
      accaaacgc gacgaaatc gccagtgcc aacaccaac 
      accgaggag cgcaaagcc cgcaaacgc aaagaacga 
      ggtgacacc aaaaacgac aaaaaaatg aaaaaatgc 
      ggggactgt atggggtgt taccgcacc gaggactgc 
      gggcggtgc gacgtgtgc acgaggcgg agccgtcac 
      ggctccccg cgcaacaag gagcggtgc aagcagcgg 
      atttgcata aattacggc ggggggagg cgcaagagg 
      cgggattca agctcggac agggaaggc ccgcccaat 
      gcccacctc atgaccgac tatccgcgc caatgctac 
      gggcccacg tgtaccaat agcgcccgc tacggctcg 
      aaatactgc tcggatcag tgtggctta aagttggca 
      accaatcgg attttgcaa gtgttgcca cagcgtatc 
      caagagtgg gccctgagt ccctgctct gccgaagag 
      tccaatatc aaagctctc gaccaagtc aggaagcaa 
      cagttcgaa gtcaaacag attttgcaa gagttggac 
      agccggcat cgcgaattg gacgcgatt atcgagcgc 
      gccaagagc gccaccatt gatccgaac gccgatttc 
      gacaacgac gaagagtcg gaagtgtcg accatgtat 
      tgcataaca tgcggactc gaaatacat tcgaaaacg 
      gccatcaaa cacatggaa aagtgtttc aacaagtac 
      gaatctcaa gcgagtttc gggagtatt tttaaaacc 
      agaattgag gggaataac atgttttgc gatttttat 
      aatccagct aatcggact tattgtaaa agattgagg 
      gttttgtgc ccagagcat tgcaaggat ccgaaaatc 
      ggcgaatat gaagtctgt ggctgtcca ttggtcacc 
      aacgttttc gatctgaca ggggagttt tgtcgagcg 
      ccgaaaaaa tcatgcgtt aagcatcac gtttgggag 
      aaattgaga cgggctgag atcgacttg gaacgggtg 
      cgccagtgg ttgaaaatg gacgaactt ttagaaaaa 
      gagcgccaa attcgcaac acaatggcc tcgagagcc 
      ggagtgctc tctctgatg ctgcactcg acatacaat 
      cacgaagtc atggaacaa ataacaaaa gcgagtcag 
      caggaccaa aacgcgatg caaagagag ttggctaag 
      agattccgc acgtctacc aaaccacgt ttcacgtaa 
      aaactttat ttaagaaaa tatgtacaa catt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY