Status
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Gene information
General information
TC013689 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5954955..5980251
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013689
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05477

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    head slightlyorientation irregular - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013689 RA
      MRMHTVSPS PANVATTTT IPNGDASND NQDRYCAEC 
      DIRFSSQKT YRAHKMHYC NSRHIVKSS AKTTSSCTS 
      GSSPTSPVD TTCRTPPSP GSQIPQQPF LALPTNPII 
      IVPYSLFRS ASVLPGILP SAAGLPNPD TPCFLLPNG 
      TLQPMTQAI NHNPPPQPP EVLKSVNKP KENNREATS 
      APLDLSIRK SPELKNLVI DLGDDHEKE NVKNRSPTP 
      EKIECVPSI HGSPVSQTG VSPVPSVSP KRKHEETSR 
      SNSPRLSRL TPKSNSTTE RSRTSPETN PPIFDIPPG 
      LHPLLMRAG SLSLFSPEV QMRLAELPA LPPVAPQVL 
      VKQGVSKCK ECNIVFCKH ENYVIHKKH YCSARSQED 
      DGSKTSGSP PVSPRSAGT TSPAGQYQQ LICLACGIK 
      FTSLDNLNA HQAYYCLKR GELDVRRCG KCRGIAEPG 
      HQCIPAGAL SGWKCPCCD VVSPTASAA QRHMESHTG 
      VKAYRCTIC RYKGNTLRG MRTHIRMHF DKRSPDLQE 
      EKYITYILE DDGANVMEV NVSPATSAP GVTDDRAAS 
      PSSEGRVEA LHHCTQCMY SSPYKANVL RHAKIAHSP 
      REPEEVAVN GSSEKATTR SMPSLDDDD IVIKKEAIE 
      PEVIIAPID DSPKEKNSD AELKKKEQE EEILQEASK 
      AGPKYCKSC DISFNYYST FIAHKKFYC SSHAGEITA 
      ATANNNNNS TRAAETSVL
    CDS sequence
    • >TC013689 RA
      atgcggatg catacggtg tcccctagt cctgccaat 
      gtggccaca accacaacc atacccaac ggtgacgct 
      tcaaacgat aatcaggac cgttactgc gccgaatgc 
      gacattcgt ttcagttcg caaaaaacc tacagggct 
      cataaaatg cactactgc aattcgagg catattgtc 
      aagtcctca gcgaaaaca acctcgagt tgcacctca 
      ggatcgtca ccaacgtca ccagttgac acaacctgt 
      cgaacacct ccatccccc ggcagtcaa atcccccaa 
      caacccttc ctagcatta ccaactaac cccatcata 
      attgtgcca tactcccta ttccgcagc gctagcgtt 
      cttccaggt attcttcca tcagctgcc ggcttaccc 
      aaccccgac accccctgc ttcctccta cccaacgga 
      accttgcaa cctatgacc caagccatc aaccacaac 
      ccgcccccg caacccccc gaagtctta aaatcagtc 
      aacaagccc aaggaaaat aacagagaa gcaacttca 
      gcaccgcta gatctcagt atacgaaaa tctcccgaa 
      ctcaagaac ctcgtcatc gacctaggt gacgaccac 
      gagaaggaa aacgtgaaa aaccgatcg ccaaccccc 
      gaaaaaatc gaatgtgtg ccatcaatc cacggatca 
      ccagtatcc caaaccggg gtctccccc gtgccaagc 
      gtctccccc aagcgcaaa cacgaggaa acctccagg 
      agcaacagt ccgcgctta tcccgtctg accccaaaa 
      tccaactcg acaaccgag cgatctcga actagtcca 
      gaaaccaac cccccaatc ttcgacatt cccccaggc 
      ctgcacccc ctcctgatg cgagccggt tcgctctcc 
      ctcttctcc cccgaagtc caaatgcga cttgctgag 
      ctcccagcc ttgcccccc gtcgccccc caagtgctg 
      gtcaagcaa ggagtctcg aaatgcaag gaatgtaac 
      atagtattc tgcaagcac gagaactat gtcatccat 
      aaaaagcac tactgctcg gcgcgaagt caggaagac 
      gatgggtcg aaaacatca ggaagtcct cctgtaagt 
      ccccggagt gcgggtact acgagtcct gcgggacag 
      taccaacag ctgatttgc ttggcttgt ggaatcaag 
      ttcacgtct ttggataat ctcaacgcg catcaagcg 
      tactattgt ttgaaacgg ggggagttg gatgtgagg 
      aggtgcggg aagtgtaga gggattgca gagcctgga 
      catcagtgc atccctgcg ggggcgctg tcgggatgg 
      aagtgtccg tgctgtgat gtggtcagt ccgacggcc 
      agtgccgcc caaaggcat atggagtcc cacacgggg 
      gtcaaggcg tacagatgc accatttgc agatacaaa 
      ggcaacact ttgagggga atgcgcaca catattagg 
      atgcatttc gataaacgc tcgccagat ttacaggag 
      gaaaagtac ataacgtac atcttggaa gacgacggc 
      gcaaatgtg atggaggtg aacgtgtct ccggcgacg 
      agcgccccc ggcgtgacc gacgatcgg gcggcgtct 
      cccagctcg gaggggcgc gtggaggca ttgcaccac 
      tgcacccaa tgcatgtac agctcccct tacaaagcg 
      aacgttttg cggcatgcg aagatcgcg cacagcccc 
      cgcgagccc gaggaggtg gccgtgaac ggctcctcc 
      gagaaggcc accacccgg tcgatgccg tcgctggac 
      gacgatgac atcgtcatc aaaaaggag gccatagag 
      cccgaagtc atcatcgca ccgattgac gattcgccg 
      aaagagaaa aactcggac gctgagttg aagaaaaag 
      gagcaagag gaggaaatt ctgcaagag gcgtcgaaa 
      gccggcccg aagtactgc aagtcttgt gatatatct 
      tttaattac tattcgacg ttcatcgcc cataagaag 
      ttctactgt tcgagccat gcgggcgaa ataaccgcc 
      gccactgcg aataacaat aataattcg acacgagcg 
      gcagaaact tcggtattg taa
    mRNA sequence
    • >TC013689 RA
      ttagtagta aagttaata tttccatac tagtttcgg 
      ctatgagta gagtttcca tcaaacgca gcttggatg 
      tgtcctgca tgcctcatc accgcgaaa ttctaaatc 
      ggaggcgga taattgcga cttgaagat gttagacac 
      gtgccagtg gcaggccgg ctatggtcc taccaaact 
      tctaatgaa ccggtactt gaagtccgg gcattacaa 
      gttcgctag gatcaggaa taagagtgc gacgacact 
      cgtccgtta acatttgca ttttgtttc aggggagga 
      agaatgggt gaataacac cgacgaaaa cactactgc 
      taccaccga aaaagacaa acccccatc cgccacaac 
      tccggccag aagcaaaac gcccgaaaa atccgacga 
      tgatgaccc caaacccac cacaatcaa aacggaggc 
      tgattcgtc accgcctcg aatcagact caaagcgaa 
      tcttgcgac cgaccccgc gcttcaatc acacgtcac 
      cattcctag tctcaaaac cgaaatcga gacgagtca 
      gtctcccag cgaatatct cgctgcgct tccttccgc 
      cattcaaaa cgcacttgc ctcccgagg ctttttctt 
      gcccaattt gagcgaaac tcgcccaag cgaaacctc 
      ggaagtcgc aagacagcc aagcgttcc cctcttcat 
      ttgtccacc gtgcgggat ccgcttttc gtcgttgag 
      tactttgga agcgcacca aacgtacta ttgctctca 
      tagagtgaa caaacccac ggaggatga tgcaaaatc 
      agtgactgg agtcgaacc aagtgggag tcagtccga 
      ccaagaccc aaacgaacc cacaaccaa aaacattcg 
      caccgggaa acagtacac ctgtacgca ttgcaccta 
      cagtgctga taagaaagt gagtctcaa tagacacat 
      gcggatgca tacggtgtc ccctagtcc tgccaatgt 
      ggccacaac cacaaccat acccaacgg tgacgcttc 
      aaacgataa tcaggaccg ttactgcgc cgaatgcga 
      cattcgttt cagttcgca aaaaaccta cagggctca 
      taaaatgca ctactgcaa ttcgaggca tattgtcaa 
      gtcctcagc gaaaacaac ctcgagttg cacctcagg 
      atcgtcacc aacgtcacc agttgacac aacctgtcg 
      aacacctcc atcccccgg cagtcaaat cccccaaca 
      acccttcct agcattacc aactaaccc catcataat 
      tgtgccata ctccctatt ccgcagcgc tagcgttct 
      tccaggtat tcttccatc agctgccgg cttacccaa 
      ccccgacac cccctgctt cctcctacc caacggaac 
      cttgcaacc tatgaccca agccatcaa ccacaaccc 
      gcccccgca accccccga agtcttaaa atcagtcaa 
      caagcccaa ggaaaataa cagagaagc aacttcagc 
      accgctaga tctcagtat acgaaaatc tcccgaact 
      caagaacct cgtcatcga cctaggtga cgaccacga 
      gaaggaaaa cgtgaaaaa ccgatcgcc aacccccga 
      aaaaatcga atgtgtgcc atcaatcca cggatcacc 
      agtatccca aaccggggt ctcccccgt gccaagcgt 
      ctcccccaa gcgcaaaca cgaggaaac ctccaggag 
      caacagtcc gcgcttatc ccgtctgac cccaaaatc 
      caactcgac aaccgagcg atctcgaac tagtccaga 
      aaccaaccc cccaatctt cgacattcc cccaggcct 
      gcaccccct cctgatgcg agccggttc gctctccct 
      cttctcccc cgaagtcca aatgcgact tgctgagct 
      cccagcctt gccccccgt cgcccccca agtgctggt 
      caagcaagg agtctcgaa atgcaagga atgtaacat 
      agtattctg caagcacga gaactatgt catccataa 
      aaagcacta ctgctcggc gcgaagtca ggaagacga 
      tgggtcgaa aacatcagg aagtcctcc tgtaagtcc 
      ccggagtgc gggtactac gagtcctgc gggacagta 
      ccaacagct gatttgctt ggcttgtgg aatcaagtt 
      cacgtcttt ggataatct caacgcgca tcaagcgta 
      ctattgttt gaaacgggg ggagttgga tgtgaggag 
      gtgcgggaa gtgtagagg gattgcaga gcctggaca 
      tcagtgcat ccctgcggg ggcgctgtc gggatggaa 
      gtgtccgtg ctgtgatgt ggtcagtcc gacggccag 
      tgccgccca aaggcatat ggagtccca cacgggggt 
      caaggcgta cagatgcac catttgcag atacaaagg 
      caacacttt gaggggaat gcgcacaca tattaggat 
      gcatttcga taaacgctc gccagattt acaggagga 
      aaagtacat aacgtacat cttggaaga cgacggcgc 
      aaatgtgat ggaggtgaa cgtgtctcc ggcgacgag 
      cgcccccgg cgtgaccga cgatcgggc ggcgtctcc 
      cagctcgga ggggcgcgt ggaggcatt gcaccactg 
      cacccaatg catgtacag ctcccctta caaagcgaa 
      cgttttgcg gcatgcgaa gatcgcgca cagcccccg 
      cgagcccga ggaggtggc cgtgaacgg ctcctccga 
      gaaggccac cacccggtc gatgccgtc gctggacga 
      cgatgacat cgtcatcaa aaaggaggc catagagcc 
      cgaagtcat catcgcacc gattgacga ttcgccgaa 
      agagaaaaa ctcggacgc tgagttgaa gaaaaagga 
      gcaagagga ggaaattct gcaagaggc gtcgaaagc 
      cggcccgaa gtactgcaa gtcttgtga tatatcttt 
      taattacta ttcgacgtt catcgccca taagaagtt 
      ctactgttc gagccatgc gggcgaaat aaccgccgc 
      cactgcgaa taacaataa taattcgac acgagcggc 
      agaaacttc ggtattgta aaatgtagt gttctagtg 
      aggaaacag gtgattttt aagtgttga atctgggcc 
      agtccttct gtatacgcg tacttgtta tcatgtatc 
      ttgtagatt ttcgcgacg cataatcgc atacgttac 
      attaactat gagtgtata aataagttt tcaaacttt 
      gttttgtat aaagtaaag atttatgga tatttttat 
      aggtttaag ttgaacata cctaatatg aaataataa 
      tgttatgcc aaagcagta gtgtacgat cgatttttc 
      ttttaacgg aactttgta ggtacataa gcatataag 
      cctctttca tcatatcgt ttaagggac tacgtcttc 
      agttgcaat tcaaatagc atatgtaca tgtaaattc 
      atgttatta ttatatagt tgtttggtg ttcaaaaca 
      cccaccgaa ggttatata catataaat atacagtga 
      ttgcccttc ttggagaca ataaattat ttatttgta 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY