Status
Please wait ...
Gene information
General information
TC013682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6074714..6080721
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013682
    iBeetle screen iB_05476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013682 RA
      MPLLRFRHS LYYIHVYAS TWCPEVFDN FLCWPATPP 
GQNVSLPCP ERINGVIIG AKAYRRCLE DGRWETRGD 
EKDKDKGYT NYNNCLLPQ LLDLLKMCD EIKECAQIT 
RTTRTIEMV GLSFSLISL IVSLIIFFQ YRVLKNNRT 
KIHKNLFIA TLLQVVFRL IKYVDQELK AGDRIIENT 
PILDEACIT LLEYSKTAM FTWMFIEGL YLHNVITVT 
VFQEYSYIK IYFYVGWTA PAVITAVWV WTMKMVRSN 
WGFYYFLPY YWILEGPRS TIIVVNLLF LINIIRVLI 
VKLRESHTS EIEQVRKAV RAAIFLLPL MGIANILFW 
MGYRFTQGW KLALWSYTS YFLNTFQGF FVAILYCFL 
NGEVQTAVK NSFYLHMSL RNHDYTPCR NLTLISTAP 
DPEQHIEKE STNWIRYCL KQNKKSPEE KEIRESVNT 
LPKLSDETD TTIIEDKSS ETKV
    • >TC013682 RB
      MPLLRFRHS LYYIHVYAS TWCPEVFDN FLCWPATPP 
GQNVSLPCP ERINGVIIG AKAYRRCLE DGRWETRGD 
EKDKDKGYT NYNNCLLPQ LLDLLKMCD EIKECAQIT 
RTTRTIEMV GLSFSLISL IVSLIIFFQ YRVLKNNRT 
KIHKNLFIA TLLQVVFRL IKYVDQELK AGDRIIENT 
PILDEACIT LLEYSKTAM FTWMFIEGL YLHNVITVT 
VFQEYSYIK IYFYVGWTA PAVITAVWV WTMKMVRSN 
WGFYYFLPY YWILEGPRS TIIVVNLLF LINIIRVLI 
VKLRESHTS EIEQVRKAV RAAIFLLPL MGIANILFW 
MGYRFTQGW KLALWSYTS YFLNTFQGF FVAILYCFL 
NGEVQTAVK NSFYLHMSL RNHDYTPCR NLTLISTAP 
DPEQHIEKE STNWIRYCL KQNKKSPEE KEIR
    CDS sequence
    • >TC013682 RA
      atgcccctt ttgcggttc cgacacagc ttgtattac 
atccatgtt tatgcatcc acctggtgt cctgaagtt 
tttgacaac tttttatgt tggccagcc acacctccg 
ggccaaaat gtctccctc ccttgtcca gagagaata 
aatggcgtt attatagga gctaaagcc taccggcgt 
tgtcttgag gatggcagg tgggaaacg agaggcgat 
gaaaaagat aaagacaaa gggtatact aattacaat 
aattgttta ctgccacaa cttctcgac cttttaaag 
atgtgcgat gaaataaag gaatgtgct cagattaca 
cgaacaacg agaactatc gaaatggtg ggactttcg 
ttctctttg atttcgttg attgtgtcc ctaattata 
ttttttcaa tacagggtc cttaaaaat aacaggaca 
aaaatccac aagaatcta ttcattgcc acccttttg 
caggtagtt tttcgtctt attaagtac gtcgatcaa 
gagctcaaa gcaggggat aggataatc gaaaatacg 
cccattctc gacgaagcc tgcataaca ctgctggaa 
tactcaaaa accgcaatg ttcacgtgg atgttcatc 
gaaggtctt tacctccat aacgtgata accgtcaca 
gtgtttcaa gaatattcc tatataaag atatacttt 
tacgtagga tggacagcc cctgcagtg atcaccgcc 
gtttgggtg tggacgatg aagatggtc agaagtaat 
tgggggttt tattatttt ttaccgtac tattggatt 
ttggaagga ccacgttcc accattatt gtggttaat 
cttctattc ttgataaac atcatccgt gttttaata 
gtaaaactg agggaaagc cacacaagc gaaatagaa 
caagtcaga aaagcggtc cgagctgca atattcctg 
ttgccttta atgggcatc gccaatatt ttgttctgg 
atggggtat cgatttact caaggatgg aaactggct 
ttatggtcg tacaccagt tactttctc aacactttt 
caaggattt ttcgtagcg atattatat tgcttctta 
aatggggaa gttcaaacc gctgtgaag aacagtttc 
tatttacac atgtctttg agaaaccac gactacact 
ccttgcagg aacttaacg ctgatttcc acggccccc 
gacccagaa cagcacatt gaaaaagag agcacaaac 
tggattcgc tactgcttg aagcagaac aaaaaatcg 
ccggaagaa aaggaaatc agagaatca gtcaatacg 
ttgccaaag ctatccgac gaaacagat acgacgata 
atagaagac aaaagttca gagacaaaa gtgtga
    • >TC013682 RB
      atgcccctt ttgcggttc cgacacagc ttgtattac 
atccatgtt tatgcatcc acctggtgt cctgaagtt 
tttgacaac tttttatgt tggccagcc acacctccg 
ggccaaaat gtctccctc ccttgtcca gagagaata 
aatggcgtt attatagga gctaaagcc taccggcgt 
tgtcttgag gatggcagg tgggaaacg agaggcgat 
gaaaaagat aaagacaaa gggtatact aattacaat 
aattgttta ctgccacaa cttctcgac cttttaaag 
atgtgcgat gaaataaag gaatgtgct cagattaca 
cgaacaacg agaactatc gaaatggtg ggactttcg 
ttctctttg atttcgttg attgtgtcc ctaattata 
ttttttcaa tacagggtc cttaaaaat aacaggaca 
aaaatccac aagaatcta ttcattgcc acccttttg 
caggtagtt tttcgtctt attaagtac gtcgatcaa 
gagctcaaa gcaggggat aggataatc gaaaatacg 
cccattctc gacgaagcc tgcataaca ctgctggaa 
tactcaaaa accgcaatg ttcacgtgg atgttcatc 
gaaggtctt tacctccat aacgtgata accgtcaca 
gtgtttcaa gaatattcc tatataaag atatacttt 
tacgtagga tggacagcc cctgcagtg atcaccgcc 
gtttgggtg tggacgatg aagatggtc agaagtaat 
tgggggttt tattatttt ttaccgtac tattggatt 
ttggaagga ccacgttcc accattatt gtggttaat 
cttctattc ttgataaac atcatccgt gttttaata 
gtaaaactg agggaaagc cacacaagc gaaatagaa 
caagtcaga aaagcggtc cgagctgca atattcctg 
ttgccttta atgggcatc gccaatatt ttgttctgg 
atggggtat cgatttact caaggatgg aaactggct 
ttatggtcg tacaccagt tactttctc aacactttt 
caaggattt ttcgtagcg atattatat tgcttctta 
aatggggaa gttcaaacc gctgtgaag aacagtttc 
tatttacac atgtctttg agaaaccac gactacact 
ccttgcagg aacttaacg ctgatttcc acggccccc 
gacccagaa cagcacatt gaaaaagag agcacaaac 
tggattcgc tactgcttg aagcagaac aaaaaatcg 
ccggaagaa aaggaaatc aggtaa
    mRNA sequence
    • >TC013682 RA
      aaatgtttg ttgaaaaaa aaaaaaaaa ctgacctct 
caattatcc ttaattgtt gtctgtgat aatcacatt 
tcatgaaca atctaataa accaagcct tgcttccta 
cagctttag taaacaatg ccccttttg cggttccga 
cacagcttg tattacatc catgtttat gcatccacc 
tggtgtcct gaagttttt gacaacttt ttatgttgg 
ccagccaca cctccgggc caaaatgtc tccctccct 
tgtccagag agaataaat ggcgttatt ataggagct 
aaagcctac cggcgttgt cttgaggat ggcaggtgg 
gaaacgaga ggcgatgaa aaagataaa gacaaaggg 
tatactaat tacaataat tgtttactg ccacaactt 
ctcgacctt ttaaagatg tgcgatgaa ataaaggaa 
tgtgctcag attacacga acaacgaga actatcgaa 
atggtggga ctttcgttc tctttgatt tcgttgatt 
gtgtcccta attatattt tttcaatac agggtcctt 
aaaaataac aggacaaaa atccacaag aatctattc 
attgccacc cttttgcag gtagttttt cgtcttatt 
aagtacgtc gatcaagag ctcaaagca ggggatagg 
ataatcgaa aatacgccc attctcgac gaagcctgc 
ataacactg ctggaatac tcaaaaacc gcaatgttc 
acgtggatg ttcatcgaa ggtctttac ctccataac 
gtgataacc gtcacagtg tttcaagaa tattcctat 
ataaagata tacttttac gtaggatgg acagcccct 
gcagtgatc accgccgtt tgggtgtgg acgatgaag 
atggtcaga agtaattgg gggttttat tatttttta 
ccgtactat tggattttg gaaggacca cgttccacc 
attattgtg gttaatctt ctattcttg ataaacatc 
atccgtgtt ttaatagta aaactgagg gaaagccac 
acaagcgaa atagaacaa gtcagaaaa gcggtccga 
gctgcaata ttcctgttg cctttaatg ggcatcgcc 
aatattttg ttctggatg gggtatcga tttactcaa 
ggatggaaa ctggcttta tggtcgtac accagttac 
tttctcaac acttttcaa ggatttttc gtagcgata 
ttatattgc ttcttaaat ggggaagtt caaaccgct 
gtgaagaac agtttctat ttacacatg tctttgaga 
aaccacgac tacactcct tgcaggaac ttaacgctg 
atttccacg gcccccgac ccagaacag cacattgaa 
aaagagagc acaaactgg attcgctac tgcttgaag 
cagaacaaa aaatcgccg gaagaaaag gaaatcaga 
gaatcagtc aatacgttg ccaaagcta tccgacgaa 
acagatacg acgataata gaagacaaa agttcagag 
acaaaagtg tgacaaaaa agcggaaca agtttggaa 
ccagctaat gaaaacagt gcgtggtca attcgttat 
tgaagctga gtgaagaaa aaccattcc gaagtggag 
aaccaaacg gttcatgtc gagaagtgg agccaacat 
tcgtcggtt ttttaagct gtacaaagt tgttacata 
atgtttttg taaagaaat actctactg taacttaaa 
gtattcttt gttatattg ttgtacgcc aaactgtaa 
atactacat tttctctgt gactgtctg taaaagaat 
gttaatata atatatatt tttcaagat t
    • >TC013682 RB
      aaatgtttg ttgaaaaaa aaaaaaaaa ctgacctct 
caattatcc ttaattgtt gtctgtgat aatcacatt 
tcatgaaca atctaataa accaagcct tgcttccta 
cagctttag taaacaatg ccccttttg cggttccga 
cacagcttg tattacatc catgtttat gcatccacc 
tggtgtcct gaagttttt gacaacttt ttatgttgg 
ccagccaca cctccgggc caaaatgtc tccctccct 
tgtccagag agaataaat ggcgttatt ataggagct 
aaagcctac cggcgttgt cttgaggat ggcaggtgg 
gaaacgaga ggcgatgaa aaagataaa gacaaaggg 
tatactaat tacaataat tgtttactg ccacaactt 
ctcgacctt ttaaagatg tgcgatgaa ataaaggaa 
tgtgctcag attacacga acaacgaga actatcgaa 
atggtggga ctttcgttc tctttgatt tcgttgatt 
gtgtcccta attatattt tttcaatac agggtcctt 
aaaaataac aggacaaaa atccacaag aatctattc 
attgccacc cttttgcag gtagttttt cgtcttatt 
aagtacgtc gatcaagag ctcaaagca ggggatagg 
ataatcgaa aatacgccc attctcgac gaagcctgc 
ataacactg ctggaatac tcaaaaacc gcaatgttc 
acgtggatg ttcatcgaa ggtctttac ctccataac 
gtgataacc gtcacagtg tttcaagaa tattcctat 
ataaagata tacttttac gtaggatgg acagcccct 
gcagtgatc accgccgtt tgggtgtgg acgatgaag 
atggtcaga agtaattgg gggttttat tatttttta 
ccgtactat tggattttg gaaggacca cgttccacc 
attattgtg gttaatctt ctattcttg ataaacatc 
atccgtgtt ttaatagta aaactgagg gaaagccac 
acaagcgaa atagaacaa gtcagaaaa gcggtccga 
gctgcaata ttcctgttg cctttaatg ggcatcgcc 
aatattttg ttctggatg gggtatcga tttactcaa 
ggatggaaa ctggcttta tggtcgtac accagttac 
tttctcaac acttttcaa ggatttttc gtagcgata 
ttatattgc ttcttaaat ggggaagtt caaaccgct 
gtgaagaac agtttctat ttacacatg tctttgaga 
aaccacgac tacactcct tgcaggaac ttaacgctg 
atttccacg gcccccgac ccagaacag cacattgaa 
aaagagagc acaaactgg attcgctac tgcttgaag 
cagaacaaa aaatcgccg gaagaaaag gaaatcagg 
taagagctc caagcccaa taattctgg ttagttagt 
ttgatgtca ctaatgttt ttgttgtct caagcgtat 
gtgatttga atgatcgtc gcaattccg ccattgtta 
ccgctttaa cgatggtgg aaacgaagc cacttacaa 
ggttcgttg ttgccacag tctgcttta tgttaatat 
tcttaacca aattttggt cgattgtcg cgttttatt 
tttcttaca gagaatcag tcaatacgt tgccaaagc 
tatccgacg aaacagata cgacgataa tagaagaca 
aaagttcag agtaagtaa gacattctt tcagtctga 
aactgatat taggaatac aggacaaaa gtgtgacaa 
aaaagcgga acaagtttg gaaccagct aatgaaaac 
agtgcgtgg tcaattcgt tattgaagc tgagtgaag 
aaaaaccat tccgaagtg gagaaccaa acggttcat 
gtcgagaag tggagccaa cattcgtcg gttttttaa 
gctgtacaa agttgttac ataatgttt ttgtaaaga 
aatactcta ctgtaactt aaagtattc tttgttata 
ttgttgtac gccaaactg taaatacta cattttctc 
tgtgactgt ctgtaaaag aatgttaat ataatatat 
atttttcaa gatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY