| General information | |||
| TC013682 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 6074714..6080721 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013682 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05476 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC013682 RA
MPLLRFRHS LYYIHVYAS TWCPEVFDN FLCWPATPP GQNVSLPCP ERINGVIIG AKAYRRCLE DGRWETRGD EKDKDKGYT NYNNCLLPQ LLDLLKMCD EIKECAQIT RTTRTIEMV GLSFSLISL IVSLIIFFQ YRVLKNNRT KIHKNLFIA TLLQVVFRL IKYVDQELK AGDRIIENT PILDEACIT LLEYSKTAM FTWMFIEGL YLHNVITVT VFQEYSYIK IYFYVGWTA PAVITAVWV WTMKMVRSN WGFYYFLPY YWILEGPRS TIIVVNLLF LINIIRVLI VKLRESHTS EIEQVRKAV RAAIFLLPL MGIANILFW MGYRFTQGW KLALWSYTS YFLNTFQGF FVAILYCFL NGEVQTAVK NSFYLHMSL RNHDYTPCR NLTLISTAP DPEQHIEKE STNWIRYCL KQNKKSPEE KEIRESVNT LPKLSDETD TTIIEDKSS ETKV
- >TC013682 RB
MPLLRFRHS LYYIHVYAS TWCPEVFDN FLCWPATPP GQNVSLPCP ERINGVIIG AKAYRRCLE DGRWETRGD EKDKDKGYT NYNNCLLPQ LLDLLKMCD EIKECAQIT RTTRTIEMV GLSFSLISL IVSLIIFFQ YRVLKNNRT KIHKNLFIA TLLQVVFRL IKYVDQELK AGDRIIENT PILDEACIT LLEYSKTAM FTWMFIEGL YLHNVITVT VFQEYSYIK IYFYVGWTA PAVITAVWV WTMKMVRSN WGFYYFLPY YWILEGPRS TIIVVNLLF LINIIRVLI VKLRESHTS EIEQVRKAV RAAIFLLPL MGIANILFW MGYRFTQGW KLALWSYTS YFLNTFQGF FVAILYCFL NGEVQTAVK NSFYLHMSL RNHDYTPCR NLTLISTAP DPEQHIEKE STNWIRYCL KQNKKSPEE KEIR
- >TC013682 RA
atgcccctt ttgcggttc cgacacagc ttgtattac atccatgtt tatgcatcc acctggtgt cctgaagtt tttgacaac tttttatgt tggccagcc acacctccg ggccaaaat gtctccctc ccttgtcca gagagaata aatggcgtt attatagga gctaaagcc taccggcgt tgtcttgag gatggcagg tgggaaacg agaggcgat gaaaaagat aaagacaaa gggtatact aattacaat aattgttta ctgccacaa cttctcgac cttttaaag atgtgcgat gaaataaag gaatgtgct cagattaca cgaacaacg agaactatc gaaatggtg ggactttcg ttctctttg atttcgttg attgtgtcc ctaattata ttttttcaa tacagggtc cttaaaaat aacaggaca aaaatccac aagaatcta ttcattgcc acccttttg caggtagtt tttcgtctt attaagtac gtcgatcaa gagctcaaa gcaggggat aggataatc gaaaatacg cccattctc gacgaagcc tgcataaca ctgctggaa tactcaaaa accgcaatg ttcacgtgg atgttcatc gaaggtctt tacctccat aacgtgata accgtcaca gtgtttcaa gaatattcc tatataaag atatacttt tacgtagga tggacagcc cctgcagtg atcaccgcc gtttgggtg tggacgatg aagatggtc agaagtaat tgggggttt tattatttt ttaccgtac tattggatt ttggaagga ccacgttcc accattatt gtggttaat cttctattc ttgataaac atcatccgt gttttaata gtaaaactg agggaaagc cacacaagc gaaatagaa caagtcaga aaagcggtc cgagctgca atattcctg ttgccttta atgggcatc gccaatatt ttgttctgg atggggtat cgatttact caaggatgg aaactggct ttatggtcg tacaccagt tactttctc aacactttt caaggattt ttcgtagcg atattatat tgcttctta aatggggaa gttcaaacc gctgtgaag aacagtttc tatttacac atgtctttg agaaaccac gactacact ccttgcagg aacttaacg ctgatttcc acggccccc gacccagaa cagcacatt gaaaaagag agcacaaac tggattcgc tactgcttg aagcagaac aaaaaatcg ccggaagaa aaggaaatc agagaatca gtcaatacg ttgccaaag ctatccgac gaaacagat acgacgata atagaagac aaaagttca gagacaaaa gtgtga
- >TC013682 RB
atgcccctt ttgcggttc cgacacagc ttgtattac atccatgtt tatgcatcc acctggtgt cctgaagtt tttgacaac tttttatgt tggccagcc acacctccg ggccaaaat gtctccctc ccttgtcca gagagaata aatggcgtt attatagga gctaaagcc taccggcgt tgtcttgag gatggcagg tgggaaacg agaggcgat gaaaaagat aaagacaaa gggtatact aattacaat aattgttta ctgccacaa cttctcgac cttttaaag atgtgcgat gaaataaag gaatgtgct cagattaca cgaacaacg agaactatc gaaatggtg ggactttcg ttctctttg atttcgttg attgtgtcc ctaattata ttttttcaa tacagggtc cttaaaaat aacaggaca aaaatccac aagaatcta ttcattgcc acccttttg caggtagtt tttcgtctt attaagtac gtcgatcaa gagctcaaa gcaggggat aggataatc gaaaatacg cccattctc gacgaagcc tgcataaca ctgctggaa tactcaaaa accgcaatg ttcacgtgg atgttcatc gaaggtctt tacctccat aacgtgata accgtcaca gtgtttcaa gaatattcc tatataaag atatacttt tacgtagga tggacagcc cctgcagtg atcaccgcc gtttgggtg tggacgatg aagatggtc agaagtaat tgggggttt tattatttt ttaccgtac tattggatt ttggaagga ccacgttcc accattatt gtggttaat cttctattc ttgataaac atcatccgt gttttaata gtaaaactg agggaaagc cacacaagc gaaatagaa caagtcaga aaagcggtc cgagctgca atattcctg ttgccttta atgggcatc gccaatatt ttgttctgg atggggtat cgatttact caaggatgg aaactggct ttatggtcg tacaccagt tactttctc aacactttt caaggattt ttcgtagcg atattatat tgcttctta aatggggaa gttcaaacc gctgtgaag aacagtttc tatttacac atgtctttg agaaaccac gactacact ccttgcagg aacttaacg ctgatttcc acggccccc gacccagaa cagcacatt gaaaaagag agcacaaac tggattcgc tactgcttg aagcagaac aaaaaatcg ccggaagaa aaggaaatc aggtaa
- >TC013682 RA
aaatgtttg ttgaaaaaa aaaaaaaaa ctgacctct caattatcc ttaattgtt gtctgtgat aatcacatt tcatgaaca atctaataa accaagcct tgcttccta cagctttag taaacaatg ccccttttg cggttccga cacagcttg tattacatc catgtttat gcatccacc tggtgtcct gaagttttt gacaacttt ttatgttgg ccagccaca cctccgggc caaaatgtc tccctccct tgtccagag agaataaat ggcgttatt ataggagct aaagcctac cggcgttgt cttgaggat ggcaggtgg gaaacgaga ggcgatgaa aaagataaa gacaaaggg tatactaat tacaataat tgtttactg ccacaactt ctcgacctt ttaaagatg tgcgatgaa ataaaggaa tgtgctcag attacacga acaacgaga actatcgaa atggtggga ctttcgttc tctttgatt tcgttgatt gtgtcccta attatattt tttcaatac agggtcctt aaaaataac aggacaaaa atccacaag aatctattc attgccacc cttttgcag gtagttttt cgtcttatt aagtacgtc gatcaagag ctcaaagca ggggatagg ataatcgaa aatacgccc attctcgac gaagcctgc ataacactg ctggaatac tcaaaaacc gcaatgttc acgtggatg ttcatcgaa ggtctttac ctccataac gtgataacc gtcacagtg tttcaagaa tattcctat ataaagata tacttttac gtaggatgg acagcccct gcagtgatc accgccgtt tgggtgtgg acgatgaag atggtcaga agtaattgg gggttttat tatttttta ccgtactat tggattttg gaaggacca cgttccacc attattgtg gttaatctt ctattcttg ataaacatc atccgtgtt ttaatagta aaactgagg gaaagccac acaagcgaa atagaacaa gtcagaaaa gcggtccga gctgcaata ttcctgttg cctttaatg ggcatcgcc aatattttg ttctggatg gggtatcga tttactcaa ggatggaaa ctggcttta tggtcgtac accagttac tttctcaac acttttcaa ggatttttc gtagcgata ttatattgc ttcttaaat ggggaagtt caaaccgct gtgaagaac agtttctat ttacacatg tctttgaga aaccacgac tacactcct tgcaggaac ttaacgctg atttccacg gcccccgac ccagaacag cacattgaa aaagagagc acaaactgg attcgctac tgcttgaag cagaacaaa aaatcgccg gaagaaaag gaaatcaga gaatcagtc aatacgttg ccaaagcta tccgacgaa acagatacg acgataata gaagacaaa agttcagag acaaaagtg tgacaaaaa agcggaaca agtttggaa ccagctaat gaaaacagt gcgtggtca attcgttat tgaagctga gtgaagaaa aaccattcc gaagtggag aaccaaacg gttcatgtc gagaagtgg agccaacat tcgtcggtt ttttaagct gtacaaagt tgttacata atgtttttg taaagaaat actctactg taacttaaa gtattcttt gttatattg ttgtacgcc aaactgtaa atactacat tttctctgt gactgtctg taaaagaat gttaatata atatatatt tttcaagat t
- >TC013682 RB
aaatgtttg ttgaaaaaa aaaaaaaaa ctgacctct caattatcc ttaattgtt gtctgtgat aatcacatt tcatgaaca atctaataa accaagcct tgcttccta cagctttag taaacaatg ccccttttg cggttccga cacagcttg tattacatc catgtttat gcatccacc tggtgtcct gaagttttt gacaacttt ttatgttgg ccagccaca cctccgggc caaaatgtc tccctccct tgtccagag agaataaat ggcgttatt ataggagct aaagcctac cggcgttgt cttgaggat ggcaggtgg gaaacgaga ggcgatgaa aaagataaa gacaaaggg tatactaat tacaataat tgtttactg ccacaactt ctcgacctt ttaaagatg tgcgatgaa ataaaggaa tgtgctcag attacacga acaacgaga actatcgaa atggtggga ctttcgttc tctttgatt tcgttgatt gtgtcccta attatattt tttcaatac agggtcctt aaaaataac aggacaaaa atccacaag aatctattc attgccacc cttttgcag gtagttttt cgtcttatt aagtacgtc gatcaagag ctcaaagca ggggatagg ataatcgaa aatacgccc attctcgac gaagcctgc ataacactg ctggaatac tcaaaaacc gcaatgttc acgtggatg ttcatcgaa ggtctttac ctccataac gtgataacc gtcacagtg tttcaagaa tattcctat ataaagata tacttttac gtaggatgg acagcccct gcagtgatc accgccgtt tgggtgtgg acgatgaag atggtcaga agtaattgg gggttttat tatttttta ccgtactat tggattttg gaaggacca cgttccacc attattgtg gttaatctt ctattcttg ataaacatc atccgtgtt ttaatagta aaactgagg gaaagccac acaagcgaa atagaacaa gtcagaaaa gcggtccga gctgcaata ttcctgttg cctttaatg ggcatcgcc aatattttg ttctggatg gggtatcga tttactcaa ggatggaaa ctggcttta tggtcgtac accagttac tttctcaac acttttcaa ggatttttc gtagcgata ttatattgc ttcttaaat ggggaagtt caaaccgct gtgaagaac agtttctat ttacacatg tctttgaga aaccacgac tacactcct tgcaggaac ttaacgctg atttccacg gcccccgac ccagaacag cacattgaa aaagagagc acaaactgg attcgctac tgcttgaag cagaacaaa aaatcgccg gaagaaaag gaaatcagg taagagctc caagcccaa taattctgg ttagttagt ttgatgtca ctaatgttt ttgttgtct caagcgtat gtgatttga atgatcgtc gcaattccg ccattgtta ccgctttaa cgatggtgg aaacgaagc cacttacaa ggttcgttg ttgccacag tctgcttta tgttaatat tcttaacca aattttggt cgattgtcg cgttttatt tttcttaca gagaatcag tcaatacgt tgccaaagc tatccgacg aaacagata cgacgataa tagaagaca aaagttcag agtaagtaa gacattctt tcagtctga aactgatat taggaatac aggacaaaa gtgtgacaa aaaagcgga acaagtttg gaaccagct aatgaaaac agtgcgtgg tcaattcgt tattgaagc tgagtgaag aaaaaccat tccgaagtg gagaaccaa acggttcat gtcgagaag tggagccaa cattcgtcg gttttttaa gctgtacaa agttgttac ataatgttt ttgtaaaga aatactcta ctgtaactt aaagtattc tttgttata ttgttgtac gccaaactg taaatacta cattttctc tgtgactgt ctgtaaaag aatgttaat ataatatat atttttcaa gatt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
