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Gene information
General information
TC013682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6074714..6080721
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013682
    iBeetle screen iB_05476

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013682 RA
      MPLLRFRHS LYYIHVYAS TWCPEVFDN FLCWPATPP 
      GQNVSLPCP ERINGVIIG AKAYRRCLE DGRWETRGD 
      EKDKDKGYT NYNNCLLPQ LLDLLKMCD EIKECAQIT 
      RTTRTIEMV GLSFSLISL IVSLIIFFQ YRVLKNNRT 
      KIHKNLFIA TLLQVVFRL IKYVDQELK AGDRIIENT 
      PILDEACIT LLEYSKTAM FTWMFIEGL YLHNVITVT 
      VFQEYSYIK IYFYVGWTA PAVITAVWV WTMKMVRSN 
      WGFYYFLPY YWILEGPRS TIIVVNLLF LINIIRVLI 
      VKLRESHTS EIEQVRKAV RAAIFLLPL MGIANILFW 
      MGYRFTQGW KLALWSYTS YFLNTFQGF FVAILYCFL 
      NGEVQTAVK NSFYLHMSL RNHDYTPCR NLTLISTAP 
      DPEQHIEKE STNWIRYCL KQNKKSPEE KEIRESVNT 
      LPKLSDETD TTIIEDKSS ETKV
    • >TC013682 RB
      MPLLRFRHS LYYIHVYAS TWCPEVFDN FLCWPATPP 
      GQNVSLPCP ERINGVIIG AKAYRRCLE DGRWETRGD 
      EKDKDKGYT NYNNCLLPQ LLDLLKMCD EIKECAQIT 
      RTTRTIEMV GLSFSLISL IVSLIIFFQ YRVLKNNRT 
      KIHKNLFIA TLLQVVFRL IKYVDQELK AGDRIIENT 
      PILDEACIT LLEYSKTAM FTWMFIEGL YLHNVITVT 
      VFQEYSYIK IYFYVGWTA PAVITAVWV WTMKMVRSN 
      WGFYYFLPY YWILEGPRS TIIVVNLLF LINIIRVLI 
      VKLRESHTS EIEQVRKAV RAAIFLLPL MGIANILFW 
      MGYRFTQGW KLALWSYTS YFLNTFQGF FVAILYCFL 
      NGEVQTAVK NSFYLHMSL RNHDYTPCR NLTLISTAP 
      DPEQHIEKE STNWIRYCL KQNKKSPEE KEIR
    CDS sequence
    • >TC013682 RA
      atgcccctt ttgcggttc cgacacagc ttgtattac 
      atccatgtt tatgcatcc acctggtgt cctgaagtt 
      tttgacaac tttttatgt tggccagcc acacctccg 
      ggccaaaat gtctccctc ccttgtcca gagagaata 
      aatggcgtt attatagga gctaaagcc taccggcgt 
      tgtcttgag gatggcagg tgggaaacg agaggcgat 
      gaaaaagat aaagacaaa gggtatact aattacaat 
      aattgttta ctgccacaa cttctcgac cttttaaag 
      atgtgcgat gaaataaag gaatgtgct cagattaca 
      cgaacaacg agaactatc gaaatggtg ggactttcg 
      ttctctttg atttcgttg attgtgtcc ctaattata 
      ttttttcaa tacagggtc cttaaaaat aacaggaca 
      aaaatccac aagaatcta ttcattgcc acccttttg 
      caggtagtt tttcgtctt attaagtac gtcgatcaa 
      gagctcaaa gcaggggat aggataatc gaaaatacg 
      cccattctc gacgaagcc tgcataaca ctgctggaa 
      tactcaaaa accgcaatg ttcacgtgg atgttcatc 
      gaaggtctt tacctccat aacgtgata accgtcaca 
      gtgtttcaa gaatattcc tatataaag atatacttt 
      tacgtagga tggacagcc cctgcagtg atcaccgcc 
      gtttgggtg tggacgatg aagatggtc agaagtaat 
      tgggggttt tattatttt ttaccgtac tattggatt 
      ttggaagga ccacgttcc accattatt gtggttaat 
      cttctattc ttgataaac atcatccgt gttttaata 
      gtaaaactg agggaaagc cacacaagc gaaatagaa 
      caagtcaga aaagcggtc cgagctgca atattcctg 
      ttgccttta atgggcatc gccaatatt ttgttctgg 
      atggggtat cgatttact caaggatgg aaactggct 
      ttatggtcg tacaccagt tactttctc aacactttt 
      caaggattt ttcgtagcg atattatat tgcttctta 
      aatggggaa gttcaaacc gctgtgaag aacagtttc 
      tatttacac atgtctttg agaaaccac gactacact 
      ccttgcagg aacttaacg ctgatttcc acggccccc 
      gacccagaa cagcacatt gaaaaagag agcacaaac 
      tggattcgc tactgcttg aagcagaac aaaaaatcg 
      ccggaagaa aaggaaatc agagaatca gtcaatacg 
      ttgccaaag ctatccgac gaaacagat acgacgata 
      atagaagac aaaagttca gagacaaaa gtgtga
    • >TC013682 RB
      atgcccctt ttgcggttc cgacacagc ttgtattac 
      atccatgtt tatgcatcc acctggtgt cctgaagtt 
      tttgacaac tttttatgt tggccagcc acacctccg 
      ggccaaaat gtctccctc ccttgtcca gagagaata 
      aatggcgtt attatagga gctaaagcc taccggcgt 
      tgtcttgag gatggcagg tgggaaacg agaggcgat 
      gaaaaagat aaagacaaa gggtatact aattacaat 
      aattgttta ctgccacaa cttctcgac cttttaaag 
      atgtgcgat gaaataaag gaatgtgct cagattaca 
      cgaacaacg agaactatc gaaatggtg ggactttcg 
      ttctctttg atttcgttg attgtgtcc ctaattata 
      ttttttcaa tacagggtc cttaaaaat aacaggaca 
      aaaatccac aagaatcta ttcattgcc acccttttg 
      caggtagtt tttcgtctt attaagtac gtcgatcaa 
      gagctcaaa gcaggggat aggataatc gaaaatacg 
      cccattctc gacgaagcc tgcataaca ctgctggaa 
      tactcaaaa accgcaatg ttcacgtgg atgttcatc 
      gaaggtctt tacctccat aacgtgata accgtcaca 
      gtgtttcaa gaatattcc tatataaag atatacttt 
      tacgtagga tggacagcc cctgcagtg atcaccgcc 
      gtttgggtg tggacgatg aagatggtc agaagtaat 
      tgggggttt tattatttt ttaccgtac tattggatt 
      ttggaagga ccacgttcc accattatt gtggttaat 
      cttctattc ttgataaac atcatccgt gttttaata 
      gtaaaactg agggaaagc cacacaagc gaaatagaa 
      caagtcaga aaagcggtc cgagctgca atattcctg 
      ttgccttta atgggcatc gccaatatt ttgttctgg 
      atggggtat cgatttact caaggatgg aaactggct 
      ttatggtcg tacaccagt tactttctc aacactttt 
      caaggattt ttcgtagcg atattatat tgcttctta 
      aatggggaa gttcaaacc gctgtgaag aacagtttc 
      tatttacac atgtctttg agaaaccac gactacact 
      ccttgcagg aacttaacg ctgatttcc acggccccc 
      gacccagaa cagcacatt gaaaaagag agcacaaac 
      tggattcgc tactgcttg aagcagaac aaaaaatcg 
      ccggaagaa aaggaaatc aggtaa
    mRNA sequence
    • >TC013682 RA
      aaatgtttg ttgaaaaaa aaaaaaaaa ctgacctct 
      caattatcc ttaattgtt gtctgtgat aatcacatt 
      tcatgaaca atctaataa accaagcct tgcttccta 
      cagctttag taaacaatg ccccttttg cggttccga 
      cacagcttg tattacatc catgtttat gcatccacc 
      tggtgtcct gaagttttt gacaacttt ttatgttgg 
      ccagccaca cctccgggc caaaatgtc tccctccct 
      tgtccagag agaataaat ggcgttatt ataggagct 
      aaagcctac cggcgttgt cttgaggat ggcaggtgg 
      gaaacgaga ggcgatgaa aaagataaa gacaaaggg 
      tatactaat tacaataat tgtttactg ccacaactt 
      ctcgacctt ttaaagatg tgcgatgaa ataaaggaa 
      tgtgctcag attacacga acaacgaga actatcgaa 
      atggtggga ctttcgttc tctttgatt tcgttgatt 
      gtgtcccta attatattt tttcaatac agggtcctt 
      aaaaataac aggacaaaa atccacaag aatctattc 
      attgccacc cttttgcag gtagttttt cgtcttatt 
      aagtacgtc gatcaagag ctcaaagca ggggatagg 
      ataatcgaa aatacgccc attctcgac gaagcctgc 
      ataacactg ctggaatac tcaaaaacc gcaatgttc 
      acgtggatg ttcatcgaa ggtctttac ctccataac 
      gtgataacc gtcacagtg tttcaagaa tattcctat 
      ataaagata tacttttac gtaggatgg acagcccct 
      gcagtgatc accgccgtt tgggtgtgg acgatgaag 
      atggtcaga agtaattgg gggttttat tatttttta 
      ccgtactat tggattttg gaaggacca cgttccacc 
      attattgtg gttaatctt ctattcttg ataaacatc 
      atccgtgtt ttaatagta aaactgagg gaaagccac 
      acaagcgaa atagaacaa gtcagaaaa gcggtccga 
      gctgcaata ttcctgttg cctttaatg ggcatcgcc 
      aatattttg ttctggatg gggtatcga tttactcaa 
      ggatggaaa ctggcttta tggtcgtac accagttac 
      tttctcaac acttttcaa ggatttttc gtagcgata 
      ttatattgc ttcttaaat ggggaagtt caaaccgct 
      gtgaagaac agtttctat ttacacatg tctttgaga 
      aaccacgac tacactcct tgcaggaac ttaacgctg 
      atttccacg gcccccgac ccagaacag cacattgaa 
      aaagagagc acaaactgg attcgctac tgcttgaag 
      cagaacaaa aaatcgccg gaagaaaag gaaatcaga 
      gaatcagtc aatacgttg ccaaagcta tccgacgaa 
      acagatacg acgataata gaagacaaa agttcagag 
      acaaaagtg tgacaaaaa agcggaaca agtttggaa 
      ccagctaat gaaaacagt gcgtggtca attcgttat 
      tgaagctga gtgaagaaa aaccattcc gaagtggag 
      aaccaaacg gttcatgtc gagaagtgg agccaacat 
      tcgtcggtt ttttaagct gtacaaagt tgttacata 
      atgtttttg taaagaaat actctactg taacttaaa 
      gtattcttt gttatattg ttgtacgcc aaactgtaa 
      atactacat tttctctgt gactgtctg taaaagaat 
      gttaatata atatatatt tttcaagat t
    • >TC013682 RB
      aaatgtttg ttgaaaaaa aaaaaaaaa ctgacctct 
      caattatcc ttaattgtt gtctgtgat aatcacatt 
      tcatgaaca atctaataa accaagcct tgcttccta 
      cagctttag taaacaatg ccccttttg cggttccga 
      cacagcttg tattacatc catgtttat gcatccacc 
      tggtgtcct gaagttttt gacaacttt ttatgttgg 
      ccagccaca cctccgggc caaaatgtc tccctccct 
      tgtccagag agaataaat ggcgttatt ataggagct 
      aaagcctac cggcgttgt cttgaggat ggcaggtgg 
      gaaacgaga ggcgatgaa aaagataaa gacaaaggg 
      tatactaat tacaataat tgtttactg ccacaactt 
      ctcgacctt ttaaagatg tgcgatgaa ataaaggaa 
      tgtgctcag attacacga acaacgaga actatcgaa 
      atggtggga ctttcgttc tctttgatt tcgttgatt 
      gtgtcccta attatattt tttcaatac agggtcctt 
      aaaaataac aggacaaaa atccacaag aatctattc 
      attgccacc cttttgcag gtagttttt cgtcttatt 
      aagtacgtc gatcaagag ctcaaagca ggggatagg 
      ataatcgaa aatacgccc attctcgac gaagcctgc 
      ataacactg ctggaatac tcaaaaacc gcaatgttc 
      acgtggatg ttcatcgaa ggtctttac ctccataac 
      gtgataacc gtcacagtg tttcaagaa tattcctat 
      ataaagata tacttttac gtaggatgg acagcccct 
      gcagtgatc accgccgtt tgggtgtgg acgatgaag 
      atggtcaga agtaattgg gggttttat tatttttta 
      ccgtactat tggattttg gaaggacca cgttccacc 
      attattgtg gttaatctt ctattcttg ataaacatc 
      atccgtgtt ttaatagta aaactgagg gaaagccac 
      acaagcgaa atagaacaa gtcagaaaa gcggtccga 
      gctgcaata ttcctgttg cctttaatg ggcatcgcc 
      aatattttg ttctggatg gggtatcga tttactcaa 
      ggatggaaa ctggcttta tggtcgtac accagttac 
      tttctcaac acttttcaa ggatttttc gtagcgata 
      ttatattgc ttcttaaat ggggaagtt caaaccgct 
      gtgaagaac agtttctat ttacacatg tctttgaga 
      aaccacgac tacactcct tgcaggaac ttaacgctg 
      atttccacg gcccccgac ccagaacag cacattgaa 
      aaagagagc acaaactgg attcgctac tgcttgaag 
      cagaacaaa aaatcgccg gaagaaaag gaaatcagg 
      taagagctc caagcccaa taattctgg ttagttagt 
      ttgatgtca ctaatgttt ttgttgtct caagcgtat 
      gtgatttga atgatcgtc gcaattccg ccattgtta 
      ccgctttaa cgatggtgg aaacgaagc cacttacaa 
      ggttcgttg ttgccacag tctgcttta tgttaatat 
      tcttaacca aattttggt cgattgtcg cgttttatt 
      tttcttaca gagaatcag tcaatacgt tgccaaagc 
      tatccgacg aaacagata cgacgataa tagaagaca 
      aaagttcag agtaagtaa gacattctt tcagtctga 
      aactgatat taggaatac aggacaaaa gtgtgacaa 
      aaaagcgga acaagtttg gaaccagct aatgaaaac 
      agtgcgtgg tcaattcgt tattgaagc tgagtgaag 
      aaaaaccat tccgaagtg gagaaccaa acggttcat 
      gtcgagaag tggagccaa cattcgtcg gttttttaa 
      gctgtacaa agttgttac ataatgttt ttgtaaaga 
      aatactcta ctgtaactt aaagtattc tttgttata 
      ttgttgtac gccaaactg taaatacta cattttctc 
      tgtgactgt ctgtaaaag aatgttaat ataatatat 
      atttttcaa gatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY