Status
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Gene information
General information
TC013660 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6758881..6761338
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013660
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05475

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013660 RA
      MLLYKAAFL FVICSTATS KRFPSYINK CYRNNNTTK 
      TCFTEAVYT LRAHFKRGI PEFSLPPMN PLILPQVLL 
      ENEDNFNAE FKNVEIFHL DQLVIQDIG FNLSRNEFE 
      TKLIFPKLR IKSNYHVKG RILFFELDG NGPADGNFT 
      DVQVETKLQ GRRYQKGSR EYIKFCKME VKESVSKAH 
      FRFDGLFKG NMELTRQMN QIVNENVDE FLTELQPAI 
      HVAIEQTVL ALVGRIFDK FSIQELFLD
    CDS sequence
    • >TC013660 RA
      atgttatta tacaaagct gcatttctt tttgtgatt 
      tgttccact gcaacttca aagcgcttt ccatcgtat 
      ataaataag tgctaccgg aacaataac accaccaaa 
      acgtgcttc actgaagct gtttacaca ctaagagct 
      catttcaaa cgcggaata ccggaattc agtttacct 
      cccatgaat cccttaatc ctcccgcaa gtattactc 
      gaaaatgaa gacaatttt aacgctgag ttcaaaaat 
      gttgaaatt tttcacctc gatcagttg gtcatccaa 
      gacatcggg ttcaattta agcagaaat gaatttgaa 
      acgaaatta attttccca aaactgaga atcaagtcg 
      aattatcac gtcaaagga cgcattttg tttttcgaa 
      cttgacgga aacggacca gctgacgga aactttact 
      gatgttcaa gttgaaacg aaattgcaa ggacgacgg 
      taccagaaa ggcagcaga gaatatatc aagttttgt 
      aaaatggaa gttaaggaa tccgtctct aaagcacat 
      ttcaggttt gatgggctt tttaaaggc aatatggag 
      cttacaaga caaatgaat caaatcgtt aatgagaat 
      gttgacgag tttttgact gaactccag ccagctatt 
      catgtagcg attgagcaa acggtgctg gccttggtt 
      ggccgaatt ttcgacaaa ttttcaata caagagctg 
      ttcttagac tag
    mRNA sequence
    • >TC013660 RA
      tcttttcgt atttttcgt aaagatgtt attatacaa 
      agctgcatt tctttttgt gatttgttc cactgcaac 
      ttcaaagcg ctttccatc gtatataaa taagtgcta 
      ccggaacaa taacaccac caaaacgtg cttcactga 
      agctgttta cacactaag agctcattt caaacgcgg 
      aataccgga attcagttt acctcccat gaatccctt 
      aatcctccc gcaagtatt actcgaaaa tgaagacaa 
      ttttaacgc tgagttcaa aaatgttga aatttttca 
      cctcgatca gttggtcat ccaagacat cgggttcaa 
      tttaagcag aaatgaatt tgaaacgaa attaatttt 
      cccaaaact gagaatcaa gtcgaatta tcacgtcaa 
      aggacgcat tttgttttt cgaacttga cggaaacgg 
      accagctga cggaaactt tactgatgt tcaagttga 
      aacgaaatt gcaaggacg acggtacca gaaaggcag 
      cagagaata tatcaagtt ttgtaaaat ggaagttaa 
      ggaatccgt ctctaaagc acatttcag gtttgatgg 
      gctttttaa aggcaatat ggagcttac aagacaaat 
      gaatcaaat cgttaatga gaatgttga cgagttttt 
      gactgaact ccagccagc tattcatgt agcgattga 
      gcaaacggt gctggcctt ggttggccg aattttcga 
      caaattttc aatacaaga gctgttctt agactagct 
      gacttgttg atcataaga caagtaatt attgtataa 
      taaagtaat atttctttt ggtaattaa ccattgctt 
      gtgtcccaa catttcgtt aagccacaa ttaacacca 
      aaccaacgg catgtaaga atgtattct tcattagat 
      atgtttgta taacaaaat aaagctgtt taaaataat 
      aacaaatag acatactga tgtcttagt aaaaaaaac 
      aggcaatga tttttgttt caatataaa atggaaata 
      ctagcatga agaatattt tttcagtga ctttatcca 
      gctttggga cttgggaag gaaataata ataattttt 
      aaggattta attatttta tatatttca tttgaggcg 
      ttacagctt atgctgaaa gttgaaaat tattcatta 
      taatgtgca ttttacgtt attttaaat gggtttcct 
      taggatgct tcatttaaa aaaaatcag gccagactt 
      atttttaca gtgggtaat aaaacgcag ctaaatttt 
      cacattatg gtttattaa ctctactat attcagaaa 
      atggtaggc ttaaaagcc acctcatat gatctaccg 
      atcatcatc acatcatta gtttattct tatatttaa 
      ttaataata aacaatagg taatatatc aataattag 
      ttatcataa tatgtctac aagacataa cgtgaacat 
      ggaaatttg acaaaatgt ttgaccacc tttaaaaat 
      aaggaaaac agaatgacg aatgggttc atggtgata 
      ataaaatgt attaaacgg gttacgtat tcatcagcg 
      cgtatggca caatttgac aaaacaaac ctcaagtaa 
      ataaaggca aggacaatc ggcatattt cttatataa 
      taataatgt aatgtaatc acacacttt atcaaatgt 
      attagttca cccacttca gggacaggc atgcagaaa 
      agtatctcc aagcggtaa aatggcccc caacacaat 
      gatattttc atataagtt atataagaa taaataatt 
      tttaacatc aacactcat tgaggcaaa aaccaaaga 
      catgtcatg tggccctag tagaaccaa acaacttcc 
      tcctacatg cctgtcaaa cattcaata ataaggaga 
      aatcataaa tccaatatc gtaattatg gttgtttaa 
      atatcatgc atacaccca tatttactt attttacca 
      tgtacgttt attgatcat tagatagaa taatatttc 
      cacgaatct ttattagtt atatttttc aaaaaataa 
      gtgtgacat ttctgcact gtttctcct tatttctga 
      ataacctcg cctctccat aggatactg caaataaat 
      tcctaaaat taactcacc acgaccttc tcgttcact 
      aacatgtag tactcatta aaatatagt cgaagattc 
      aatagcact cagggaacg agatttcgt ggaaatgct 
      taacagagc tgccaaaaa acaaatgca gaaggcctg 
      ggcctgatg tgcgtttgt ttttcaaca gacggtgca 
      aacaactag ttgtctgcg ttatgatgt gacttaaat 
      aacaatcgt tactaaaat atcacaaaa ttgttgttg 
      ataaaatga aaaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY