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Gene information
General information
TC013655 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6810070..6817578
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013655
    iBeetle screen iB_05474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013655 RA
      MTAPAKTTD EPKTFIFVA DCAEGGSEH NSPYWEQSF 
      SPPYFDNTT RREVTTTVG QTAHLHCRV RNLGDRAVS 
      WIRKRDLHI LTVGILTYT NDQRFQSLH TDGSDEWTL 
      KVTSPQTRD SGIYECQVS TEPKISQAF KLNVVVSKA 
      KIVGNPELY IKSGSDINL TCIVLQTPD PPSFIYWYR 
      GGNVINYSQ RGGISVVTE KQTRTSRLL ISRALPQDS 
      GNYTCSPSS SDAASVLVH VLNGEQPAA MQHGNNSST 
      AICWTIDFV LVIFLQCFV LER
    CDS sequence
    • >TC013655 RA
      atgactgca cccgctaaa accactgac gaacctaaa 
      acatttatt tttgttgca gattgcgcg gaaggcggt 
      tcggagcac aacagtcca tactgggag caatcgttt 
      tctccgccg tattttgac aatacgact cgaagggag 
      gtcacaact acagtgggc caaacggct catttgcat 
      tgtagagtc agaaacctg ggagacaga gctgtatcg 
      tggatccgc aagcgggat ttgcacatt ctgacagtg 
      gggatcctg acttacacg aacgatcaa cgatttcag 
      tccttgcac acagatggt tcagacgag tggacgcta 
      aaggtaacg tcccctcaa actagagat tccggcatc 
      tacgagtgt caagtcagt accgaacct aaaattagc 
      caagcgttc aaactgaat gttgtagtt tcaaaggcc 
      aaaatagtg ggtaacccc gagttgtac atcaaaagt 
      ggaagtgat ataaactta acgtgcata gttctccag 
      acgccagac ccaccaagt ttcatctac tggtaccgg 
      ggaggaaac gtgattaac tattcacag cgtggtggc 
      ataagcgta gtcaccgag aagcaaaca cgaacaagt 
      cgtttgttg atttcacga gccttgccc caggacagc 
      ggcaactac acatgttcg ccctccagc agcgacgcc 
      gccagcgtc cttgtgcat gtccttaac ggggagcaa 
      ccggcagcc atgcagcac ggcaacaac agcagcacc 
      gccatttgc tggacgata gatttcgta ctcgttata 
      ttcctccag tgctttgtg ctcgaaagg tga
    mRNA sequence
    • >TC013655 RA
      atctgagaa gtaaactag atgcatgct tgttgatta 
      cgtcccgac atgactgca cccgctaaa accactgac 
      gaacctaaa acatttatt tttgttgca gattgcgcg 
      gaaggcggt tcggagcac aacagtcca tactgggag 
      caatcgttt tctccgccg tattttgac aatacgact 
      cgaagggag gtcacaact acagtgggc caaacggct 
      catttgcat tgtagagtc agaaacctg ggagacaga 
      gctgtatcg tggatccgc aagcgggat ttgcacatt 
      ctgacagtg gggatcctg acttacacg aacgatcaa 
      cgatttcag tccttgcac acagatggt tcagacgag 
      tggacgcta aaggtaacg tcccctcaa actagagat 
      tccggcatc tacgagtgt caagtcagt accgaacct 
      aaaattagc caagcgttc aaactgaat gttgtagtt 
      tcaaaggcc aaaatagtg ggtaacccc gagttgtac 
      atcaaaagt ggaagtgat ataaactta acgtgcata 
      gttctccag acgccagac ccaccaagt ttcatctac 
      tggtaccgg ggaggaaac gtgattaac tattcacag 
      cgtggtggc ataagcgta gtcaccgag aagcaaaca 
      cgaacaagt cgtttgttg atttcacga gccttgccc 
      caggacagc ggcaactac acatgttcg ccctccagc 
      agcgacgcc gccagcgtc cttgtgcat gtccttaac 
      ggggagcaa ccggcagcc atgcagcac ggcaacaac 
      agcagcacc gccatttgc tggacgata gatttcgta 
      ctcgttata ttcctccag tgctttgtg ctcgaaagg 
      tgaatgcca acggtcctc taatgcagt ctattatat 
      atcagcgat aaatgttta tcaatttga taatggtaa 
      cactgtggc cgtgtaaat atcagcgaa tgaccagtt 
      attgttgta tgtccaaca cgtatatca taatttgtt 
      tgtataatt ctgatgtac atagttgtt gctgttaaa 
      ttgttgtca tagatgaaa attacagct ggtctatat 
      catatgtgg ctgtctgat ttattgcca ttatttatc 
      acaccgata ccatatttc aatcagctc catgcatac 
      agggtgagc ccttcggcc cgacattac aaactttct 
      aactgccga cgtagacac ggcttaatt tcgcgcgga 
      tttaagtgt ggattacaa ttcagaatg gcccaaaat 
      tttaaatcc gttaaatgt aggccggaa aaaatcacc 
      ctgcatatg cttgataat cgggacttg ttctaaaga 
      acgaaaaat caaattttg ataggctga gcctgtcgg 
      tcgacaacg atcgattat tttacataa ccatgagca 
      aagtacatt tttgactaa aaaattaga acgttaact 
      gcactgaca aacttcacg caatttcta aacacattc 
      acgttttta tcgtttgat tgaaagaat cgtgccagt 
      atatagtac aatcacaaa ctttccaca ctcacgagg 
      taaacccgg tcaggggag aacgtttaa aatattcag 
      ttgtgattg agtgttttt caaccatcc tgaatgcgg 
      tgtaggcga ccatacaat tcgatatcg tgtaaaagg 
      atttgtgaa tttgagatt ataaaactg gtacatgtt 
      cctgtgttt attaatctt ccattcatc tcatttcat 
      acattttca gtcccagct atccgagct aattgggac 
      gacacctac ctctgatag cgcaaactc ttgcagttt 
      atttatata tttctattt tttgaagct cgcataata 
      cagataatc gggactcgg agaggtggg actctactg 
      taacttata atagtaata cacgttgaa tactgactg 
      tatatactt attttagta taatatcaa atttgtgtt 
      tcaaaaatg ataactagc gttagggtc gacacttga 
      agtcgcaag attcgtgtt aaattaatt taacaaatc 
      actatgcaa agttaatgt gaattaata atcaactca 
      gcccctgct tcgactaca gcctcatca acaatattg 
      catatcaca tgaccgcca cggtttaaa atctgtagc 
      taccagagt cagagtcca gaaatgcat tacttaaac 
      aatcggaaa atgccgctc aatgtgtga ttgtgattg 
      cgtgataat gttgttttc cgactgact gacgttgtc 
      acatacaaa ataattgca taaatgatg cactaaatc 
      taaattaag gcattgtag tttcaacgc actacacaa 
      taattagct aaattaagt gcatcatag cttttatct 
      tccaatgtc attcgacgt taattaata atgaaagtg 
      gtgtttaag tgatgaatt tgaggattt ccaatttcc 
      agtgttatt ttaagtcac ggcctaaca cagaacaca 
      tttattatg acgtttatt tgtacttta tgtaacata 
      catagctta ttaaatggc attccagcg atgtcgtga 
      cttgattta aactttgat gtacaattt cactgtaaa 
      atttttcta aaattgtag aaaccatta tagttttcg 
      ttgtgttag tttttatat gcgtaaaaa tatatgttt 
      aaagtttat ttatctaaa tgtagacca tttgaattt 
      gttatgtaa atattagca ataaatagg aataatgtt 
      aatataagc ttcaaaaat ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY