Status
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Gene information
General information
TC013650 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7069125..7075142
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013650
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05473

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    Pupal morphology
    wings orientation irregular - Number of animals showing the phenotype: 3

    legs partiallynecrotic - Number of animals showing the phenotype: 3

    Comment: Especially metathoracic legs are affected.
    Adult morphology
    metathoracic leg partially distalnecrotic - Number of animals showing the phenotype: 1


    metathoracic leg tibia not present - Number of animals showing the phenotype: 1


    metathoracic leg tarsus not present - Number of animals showing the phenotype: 1

    metathoracic leg pretarsus not present - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013650 RA
      MHTVMANNT CEGMDEVGL LRYVIINLV FFIGVAGNL 
AALWILYKS AKTRNKKHV LLLRCLAVN DLVAEVGMF 
SIITLQKYK LIPVYWICV GFVLLRAFG IGSGCVAFV 
MALERWLAL TRPFYYQQL VTYRILKIF VFSLWSGGA 
LLTYLPLLG FGLYYNGKC CRYRDATEL RDQIYAYMF 
LVFGTALCL CIAFCNLTV IWELSKIRP HGRVLMRRV 
SRSTMNSRV MAAKYQTPE EIAFAKLMA IICIIFVVC 
WAPQMISIP LAQFWKGST FDGIFAKMA NFLMCTYFT 
LDPYVYVLQ RYIEKKKIA LGCCWPRRS TGSITTSSI 
TAGTPTSVL LNPPSFTSP SIM
    • >TC013650 RB
      MHTVMANNT CEGMDEVGL LRYVIINLV FFIGVAGNL 
AALWILYKS AKTRNKKHV LLLRCLAVN DLVAEVGMF 
SIITLQKYK LIPVYWICV GFVLLRAFG IGSGCVAFV 
MALERWLAL TRPFYYQQL VTYRILKIF VFSLWSGGA 
LLTYLPLLG FGLYYNGKC CRYRDATEL RDQIYAYMF 
LVFGTALCL CIAFCNLTV IWELSKIRP HGRVLMRRV 
SRSTMNSRV MAAKYQTPE EIAFAKLMA IICIIFVVC 
WAPQMISIP LAQFWKGST FDGIFAKMA NFLMCTYFT 
LDPYVYVLQ RYIEKKKIA LGCCWPRRS TEENVTSLP 
AVLQSEQDE KNLYVDRYL
    CDS sequence
    • >TC013650 RA
      atgcacaca gtcatggct aataatact tgtgaaggc 
atggatgaa gtcggactg ctccggtac gtcataatc 
aacctagtc ttcttcatc ggggttgcc ggcaacctg 
gccgccctg tggatcctt tacaaatcg gccaagacc 
agaaacaaa aagcacgtt ttattgtta aggtgccta 
gcagtcaac gatttagtg gctgaagtt ggtatgttt 
tccattata actctacaa aaatacaag ttgattccc 
gtgtattgg atttgtgtt ggttttgtg ttacttcgt 
gcttttgga atcggatcg ggatgtgtt gcttttgtg 
atggccctc gagcgatgg ttggccctt acaagacct 
ttctattat caacagcta gtcacttac cgaatttta 
aaaatattt gtattctct ttgtggtcc ggaggagcc 
ttattaact taccttccc cttcttggt ttcggattg 
tactacaac ggtaaatgt tgcagatac agagacgca 
acagaactt cgagatcaa atatatgct tacatgttc 
ttggttttc ggtactgcg ttatgcttg tgcatagca 
ttttgcaat ttaactgtt atatgggag ttatcaaaa 
atccgtcca catggccgt gttttaatg aggagggtt 
agcaggtcg acaatgaac agccgtgtt atggctgca 
aaatatcaa acccccgaa gaaatagct tttgcaaaa 
ttgatggca attatttgt atcatattt gtagtttgc 
tgggctcct caaatgatc tcaattcca ttagcccag 
ttttggaaa ggttcaact tttgatggg atatttgca 
aaaatggca aattttctc atgtgtact tactttact 
ttggacccg tacgtttac gtgttgcag cgttacatc 
gaaaaaaag aaaatagcg ctgggttgt tgctggccg 
cgccgatcc acgggtagt atcaccact tcttcaata 
acagcagga acgcccact tccgtactt ctgaatcct 
ccttccttt actagtcct tccattatg taa
    • >TC013650 RB
      atgcacaca gtcatggct aataatact tgtgaaggc 
atggatgaa gtcggactg ctccggtac gtcataatc 
aacctagtc ttcttcatc ggggttgcc ggcaacctg 
gccgccctg tggatcctt tacaaatcg gccaagacc 
agaaacaaa aagcacgtt ttattgtta aggtgccta 
gcagtcaac gatttagtg gctgaagtt ggtatgttt 
tccattata actctacaa aaatacaag ttgattccc 
gtgtattgg atttgtgtt ggttttgtg ttacttcgt 
gcttttgga atcggatcg ggatgtgtt gcttttgtg 
atggccctc gagcgatgg ttggccctt acaagacct 
ttctattat caacagcta gtcacttac cgaatttta 
aaaatattt gtattctct ttgtggtcc ggaggagcc 
ttattaact taccttccc cttcttggt ttcggattg 
tactacaac ggtaaatgt tgcagatac agagacgca 
acagaactt cgagatcaa atatatgct tacatgttc 
ttggttttc ggtactgcg ttatgcttg tgcatagca 
ttttgcaat ttaactgtt atatgggag ttatcaaaa 
atccgtcca catggccgt gttttaatg aggagggtt 
agcaggtcg acaatgaac agccgtgtt atggctgca 
aaatatcaa acccccgaa gaaatagct tttgcaaaa 
ttgatggca attatttgt atcatattt gtagtttgc 
tgggctcct caaatgatc tcaattcca ttagcccag 
ttttggaaa ggttcaact tttgatggg atatttgca 
aaaatggca aattttctc atgtgtact tactttact 
ttggacccg tacgtttac gtgttgcag cgttacatc 
gaaaaaaag aaaatagcg ctgggttgt tgctggccg 
cgccgatcc acggaagaa aacgtcaca tcgcttccg 
gccgttttg caaagcgaa caagacgaa aagaatctg 
tatgtagat agatactta taa
    mRNA sequence
    • >TC013650 RA
      ggagacctt aattatttg gggttttct caacgaaat 
tcatcaagt ggcctggca gtttccttt cgcccacca 
ccctttaaa gacgtctgg gcccaaagc agaagcagt 
aagaggtca gctgtgttg acaaacatt ttgtcataa 
tgcacacag tcatggcta ataatactt gtgaaggca 
tggatgaag tcggactgc tccggtacg tcataatca 
acctagtct tcttcatcg gggttgccg gcaacctgg 
ccgccctgt ggatccttt acaaatcgg ccaagacca 
gaaacaaaa agcacgttt tattgttaa ggtgcctag 
cagtcaacg atttagtgg ctgaagttg gtatgtttt 
ccattataa ctctacaaa aatacaagt tgattcccg 
tgtattgga tttgtgttg gttttgtgt tacttcgtg 
cttttggaa tcggatcgg gatgtgttg cttttgtga 
tggccctcg agcgatggt tggccctta caagacctt 
tctattatc aacagctag tcacttacc gaattttaa 
aaatatttg tattctctt tgtggtccg gaggagcct 
tattaactt accttcccc ttcttggtt tcggattgt 
actacaacg gtaaatgtt gcagataca gagacgcaa 
cagaacttc gagatcaaa tatatgctt acatgttct 
tggttttcg gtactgcgt tatgcttgt gcatagcat 
tttgcaatt taactgtta tatgggagt tatcaaaaa 
tccgtccac atggccgtg ttttaatga ggagggtta 
gcaggtcga caatgaaca gccgtgtta tggctgcaa 
aatatcaaa cccccgaag aaatagctt ttgcaaaat 
tgatggcaa ttatttgta tcatatttg tagtttgct 
gggctcctc aaatgatct caattccat tagcccagt 
tttggaaag gttcaactt ttgatggga tatttgcaa 
aaatggcaa attttctca tgtgtactt actttactt 
tggacccgt acgtttacg tgttgcagc gttacatcg 
aaaaaaaga aaatagcgc tgggttgtt gctggccgc 
gccgatcca cgggtagta tcaccactt cttcaataa 
cagcaggaa cgcccactt ccgtacttc tgaatcctc 
cttccttta ctagtcctt ccattatgt aaattttaa 
cctttttac aagtattta ttggtttct tcaagatat 
ttcttaatt ataccactt atctacgtt gtttagtac 
gtatatagg agtctttgt ttagaaaat tacttgacc 
caaatctcg cttgatgaa tgactcaga taagacagc 
attgcatgt tgattcaaa atttatcta ccagtaggg 
cagatggta ttacgaata cactttata aagcgaaca 
cttcaagga atatcgagt ggttttcca ggttgagtt 
aacaaaagt tgaaagcac aattttttg tgttcagaa 
tatatgaca aatgtgata gctaggagt cgtaacata 
agcagcgtt aaaacctgc tttgagatt ttaacatgg 
cttgacttg tgtattaaa acttctagt gtaaaataa 
aacgaaatt aacggagat tgttcttgg aattttctg 
atcatttca cttcataaa atatttgtt ttatcagta 
ccgacttct ccctttagt ccaaagcgt tttacttac 
actgcatgt attgtttaa ctaatttac atagttcgt 
gcgtttaaa gcgtttaca tatattcgt tatcaaagt 
taagcgaaa atgtatatc tgttgtttt ttttataga 
agaaaacgt cacatcgct tccggccgt tttgcaaag 
cgaacaaga cgaaaagaa tctgtatgt agatagata 
cttataaag tatagtatg tacacatac gctagtata 
atgtatact tattaacca aagactctt gtgcatgac 
taatttcaa tggatattt tatatgttc tttgtcgtc 
atatctatt tataataag aaacattgc aatattttc 
acagtgtct ggagaatat ttaaaaata ctgtatttt 
gtgtgtaag aacgttctt ataattatt gtagttttg 
ttgtcttta ttttgttaa tctacacgt ctgtacttt 
agccattct aacttctat gcatttggc tctgatagg 
tggccacct ctgtgtagg tataaagac gtaactgtt 
tgtaaatat atggtaaac tggtgctaa cagattgtt 
agaggacta gttgactgt gtatgtaaa attgttttt 
ggagacaag aacacggta ttgtatttt tgttat
    • >TC013650 RB
      ggagacctt aattatttg gggttttct caacgaaat 
tcatcaagt ggcctggca gtttccttt cgcccacca 
ccctttaaa gacgtctgg gcccaaagc agaagcagt 
aagaggtca gctgtgttg acaaacatt ttgtcataa 
tgcacacag tcatggcta ataatactt gtgaaggca 
tggatgaag tcggactgc tccggtacg tcataatca 
acctagtct tcttcatcg gggttgccg gcaacctgg 
ccgccctgt ggatccttt acaaatcgg ccaagacca 
gaaacaaaa agcacgttt tattgttaa ggtgcctag 
cagtcaacg atttagtgg ctgaagttg gtatgtttt 
ccattataa ctctacaaa aatacaagt tgattcccg 
tgtattgga tttgtgttg gttttgtgt tacttcgtg 
cttttggaa tcggatcgg gatgtgttg cttttgtga 
tggccctcg agcgatggt tggccctta caagacctt 
tctattatc aacagctag tcacttacc gaattttaa 
aaatatttg tattctctt tgtggtccg gaggagcct 
tattaactt accttcccc ttcttggtt tcggattgt 
actacaacg gtaaatgtt gcagataca gagacgcaa 
cagaacttc gagatcaaa tatatgctt acatgttct 
tggttttcg gtactgcgt tatgcttgt gcatagcat 
tttgcaatt taactgtta tatgggagt tatcaaaaa 
tccgtccac atggccgtg ttttaatga ggagggtta 
gcaggtcga caatgaaca gccgtgtta tggctgcaa 
aatatcaaa cccccgaag aaatagctt ttgcaaaat 
tgatggcaa ttatttgta tcatatttg tagtttgct 
gggctcctc aaatgatct caattccat tagcccagt 
tttggaaag gttcaactt ttgatggga tatttgcaa 
aaatggcaa attttctca tgtgtactt actttactt 
tggacccgt acgtttacg tgttgcagc gttacatcg 
aaaaaaaga aaatagcgc tgggttgtt gctggccgc 
gccgatcca cggaagaaa acgtcacat cgcttccgg 
ccgttttgc aaagcgaac aagacgaaa agaatctgt 
atgtagata gatacttat aaagtatag tatgtacac 
atacgctag tataatgta tacttatta accaaagac 
tcttgtgca tgactaatt tcaatggat attttatat 
gttctttgt cgtcatatc tatttataa taagaaaca 
ttgcaatat tttcacagt gtctggaga atatttaaa 
aatactgta ttttgtgtg taagaacgt tcttataat 
tattgtagt tttgttgtc tttattttg ttaatctac 
acgtctgta ctttagcca ttctaactt ctatgcatt 
tggctctga taggtggcc acctctgtg taggtataa 
agacgtaac tgtttgtaa atatatggt aaactggtg 
ctaacagat tgttagagg actagttga ctgtgtatg 
taaaattgt ttttggaga caagaacac ggtattgta 
tttttgtta t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY