Status
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Gene information
General information
TC013650 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7069125..7075142
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013650
    iBeetle screen iB_05473

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    wings orientation irregular - Number of animals showing the phenotype: 3

    legs partiallynecrotic - Number of animals showing the phenotype: 3

    Comment: Especially metathoracic legs are affected.
    Adult morphology
    metathoracic leg partially distalnecrotic - Number of animals showing the phenotype: 1


    metathoracic leg tibia not present - Number of animals showing the phenotype: 1


    metathoracic leg tarsus not present - Number of animals showing the phenotype: 1

    metathoracic leg pretarsus not present - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013650 RA
      MHTVMANNT CEGMDEVGL LRYVIINLV FFIGVAGNL 
      AALWILYKS AKTRNKKHV LLLRCLAVN DLVAEVGMF 
      SIITLQKYK LIPVYWICV GFVLLRAFG IGSGCVAFV 
      MALERWLAL TRPFYYQQL VTYRILKIF VFSLWSGGA 
      LLTYLPLLG FGLYYNGKC CRYRDATEL RDQIYAYMF 
      LVFGTALCL CIAFCNLTV IWELSKIRP HGRVLMRRV 
      SRSTMNSRV MAAKYQTPE EIAFAKLMA IICIIFVVC 
      WAPQMISIP LAQFWKGST FDGIFAKMA NFLMCTYFT 
      LDPYVYVLQ RYIEKKKIA LGCCWPRRS TGSITTSSI 
      TAGTPTSVL LNPPSFTSP SIM
    • >TC013650 RB
      MHTVMANNT CEGMDEVGL LRYVIINLV FFIGVAGNL 
      AALWILYKS AKTRNKKHV LLLRCLAVN DLVAEVGMF 
      SIITLQKYK LIPVYWICV GFVLLRAFG IGSGCVAFV 
      MALERWLAL TRPFYYQQL VTYRILKIF VFSLWSGGA 
      LLTYLPLLG FGLYYNGKC CRYRDATEL RDQIYAYMF 
      LVFGTALCL CIAFCNLTV IWELSKIRP HGRVLMRRV 
      SRSTMNSRV MAAKYQTPE EIAFAKLMA IICIIFVVC 
      WAPQMISIP LAQFWKGST FDGIFAKMA NFLMCTYFT 
      LDPYVYVLQ RYIEKKKIA LGCCWPRRS TEENVTSLP 
      AVLQSEQDE KNLYVDRYL
    CDS sequence
    • >TC013650 RA
      atgcacaca gtcatggct aataatact tgtgaaggc 
      atggatgaa gtcggactg ctccggtac gtcataatc 
      aacctagtc ttcttcatc ggggttgcc ggcaacctg 
      gccgccctg tggatcctt tacaaatcg gccaagacc 
      agaaacaaa aagcacgtt ttattgtta aggtgccta 
      gcagtcaac gatttagtg gctgaagtt ggtatgttt 
      tccattata actctacaa aaatacaag ttgattccc 
      gtgtattgg atttgtgtt ggttttgtg ttacttcgt 
      gcttttgga atcggatcg ggatgtgtt gcttttgtg 
      atggccctc gagcgatgg ttggccctt acaagacct 
      ttctattat caacagcta gtcacttac cgaatttta 
      aaaatattt gtattctct ttgtggtcc ggaggagcc 
      ttattaact taccttccc cttcttggt ttcggattg 
      tactacaac ggtaaatgt tgcagatac agagacgca 
      acagaactt cgagatcaa atatatgct tacatgttc 
      ttggttttc ggtactgcg ttatgcttg tgcatagca 
      ttttgcaat ttaactgtt atatgggag ttatcaaaa 
      atccgtcca catggccgt gttttaatg aggagggtt 
      agcaggtcg acaatgaac agccgtgtt atggctgca 
      aaatatcaa acccccgaa gaaatagct tttgcaaaa 
      ttgatggca attatttgt atcatattt gtagtttgc 
      tgggctcct caaatgatc tcaattcca ttagcccag 
      ttttggaaa ggttcaact tttgatggg atatttgca 
      aaaatggca aattttctc atgtgtact tactttact 
      ttggacccg tacgtttac gtgttgcag cgttacatc 
      gaaaaaaag aaaatagcg ctgggttgt tgctggccg 
      cgccgatcc acgggtagt atcaccact tcttcaata 
      acagcagga acgcccact tccgtactt ctgaatcct 
      ccttccttt actagtcct tccattatg taa
    • >TC013650 RB
      atgcacaca gtcatggct aataatact tgtgaaggc 
      atggatgaa gtcggactg ctccggtac gtcataatc 
      aacctagtc ttcttcatc ggggttgcc ggcaacctg 
      gccgccctg tggatcctt tacaaatcg gccaagacc 
      agaaacaaa aagcacgtt ttattgtta aggtgccta 
      gcagtcaac gatttagtg gctgaagtt ggtatgttt 
      tccattata actctacaa aaatacaag ttgattccc 
      gtgtattgg atttgtgtt ggttttgtg ttacttcgt 
      gcttttgga atcggatcg ggatgtgtt gcttttgtg 
      atggccctc gagcgatgg ttggccctt acaagacct 
      ttctattat caacagcta gtcacttac cgaatttta 
      aaaatattt gtattctct ttgtggtcc ggaggagcc 
      ttattaact taccttccc cttcttggt ttcggattg 
      tactacaac ggtaaatgt tgcagatac agagacgca 
      acagaactt cgagatcaa atatatgct tacatgttc 
      ttggttttc ggtactgcg ttatgcttg tgcatagca 
      ttttgcaat ttaactgtt atatgggag ttatcaaaa 
      atccgtcca catggccgt gttttaatg aggagggtt 
      agcaggtcg acaatgaac agccgtgtt atggctgca 
      aaatatcaa acccccgaa gaaatagct tttgcaaaa 
      ttgatggca attatttgt atcatattt gtagtttgc 
      tgggctcct caaatgatc tcaattcca ttagcccag 
      ttttggaaa ggttcaact tttgatggg atatttgca 
      aaaatggca aattttctc atgtgtact tactttact 
      ttggacccg tacgtttac gtgttgcag cgttacatc 
      gaaaaaaag aaaatagcg ctgggttgt tgctggccg 
      cgccgatcc acggaagaa aacgtcaca tcgcttccg 
      gccgttttg caaagcgaa caagacgaa aagaatctg 
      tatgtagat agatactta taa
    mRNA sequence
    • >TC013650 RA
      ggagacctt aattatttg gggttttct caacgaaat 
      tcatcaagt ggcctggca gtttccttt cgcccacca 
      ccctttaaa gacgtctgg gcccaaagc agaagcagt 
      aagaggtca gctgtgttg acaaacatt ttgtcataa 
      tgcacacag tcatggcta ataatactt gtgaaggca 
      tggatgaag tcggactgc tccggtacg tcataatca 
      acctagtct tcttcatcg gggttgccg gcaacctgg 
      ccgccctgt ggatccttt acaaatcgg ccaagacca 
      gaaacaaaa agcacgttt tattgttaa ggtgcctag 
      cagtcaacg atttagtgg ctgaagttg gtatgtttt 
      ccattataa ctctacaaa aatacaagt tgattcccg 
      tgtattgga tttgtgttg gttttgtgt tacttcgtg 
      cttttggaa tcggatcgg gatgtgttg cttttgtga 
      tggccctcg agcgatggt tggccctta caagacctt 
      tctattatc aacagctag tcacttacc gaattttaa 
      aaatatttg tattctctt tgtggtccg gaggagcct 
      tattaactt accttcccc ttcttggtt tcggattgt 
      actacaacg gtaaatgtt gcagataca gagacgcaa 
      cagaacttc gagatcaaa tatatgctt acatgttct 
      tggttttcg gtactgcgt tatgcttgt gcatagcat 
      tttgcaatt taactgtta tatgggagt tatcaaaaa 
      tccgtccac atggccgtg ttttaatga ggagggtta 
      gcaggtcga caatgaaca gccgtgtta tggctgcaa 
      aatatcaaa cccccgaag aaatagctt ttgcaaaat 
      tgatggcaa ttatttgta tcatatttg tagtttgct 
      gggctcctc aaatgatct caattccat tagcccagt 
      tttggaaag gttcaactt ttgatggga tatttgcaa 
      aaatggcaa attttctca tgtgtactt actttactt 
      tggacccgt acgtttacg tgttgcagc gttacatcg 
      aaaaaaaga aaatagcgc tgggttgtt gctggccgc 
      gccgatcca cgggtagta tcaccactt cttcaataa 
      cagcaggaa cgcccactt ccgtacttc tgaatcctc 
      cttccttta ctagtcctt ccattatgt aaattttaa 
      cctttttac aagtattta ttggtttct tcaagatat 
      ttcttaatt ataccactt atctacgtt gtttagtac 
      gtatatagg agtctttgt ttagaaaat tacttgacc 
      caaatctcg cttgatgaa tgactcaga taagacagc 
      attgcatgt tgattcaaa atttatcta ccagtaggg 
      cagatggta ttacgaata cactttata aagcgaaca 
      cttcaagga atatcgagt ggttttcca ggttgagtt 
      aacaaaagt tgaaagcac aattttttg tgttcagaa 
      tatatgaca aatgtgata gctaggagt cgtaacata 
      agcagcgtt aaaacctgc tttgagatt ttaacatgg 
      cttgacttg tgtattaaa acttctagt gtaaaataa 
      aacgaaatt aacggagat tgttcttgg aattttctg 
      atcatttca cttcataaa atatttgtt ttatcagta 
      ccgacttct ccctttagt ccaaagcgt tttacttac 
      actgcatgt attgtttaa ctaatttac atagttcgt 
      gcgtttaaa gcgtttaca tatattcgt tatcaaagt 
      taagcgaaa atgtatatc tgttgtttt ttttataga 
      agaaaacgt cacatcgct tccggccgt tttgcaaag 
      cgaacaaga cgaaaagaa tctgtatgt agatagata 
      cttataaag tatagtatg tacacatac gctagtata 
      atgtatact tattaacca aagactctt gtgcatgac 
      taatttcaa tggatattt tatatgttc tttgtcgtc 
      atatctatt tataataag aaacattgc aatattttc 
      acagtgtct ggagaatat ttaaaaata ctgtatttt 
      gtgtgtaag aacgttctt ataattatt gtagttttg 
      ttgtcttta ttttgttaa tctacacgt ctgtacttt 
      agccattct aacttctat gcatttggc tctgatagg 
      tggccacct ctgtgtagg tataaagac gtaactgtt 
      tgtaaatat atggtaaac tggtgctaa cagattgtt 
      agaggacta gttgactgt gtatgtaaa attgttttt 
      ggagacaag aacacggta ttgtatttt tgttat
    • >TC013650 RB
      ggagacctt aattatttg gggttttct caacgaaat 
      tcatcaagt ggcctggca gtttccttt cgcccacca 
      ccctttaaa gacgtctgg gcccaaagc agaagcagt 
      aagaggtca gctgtgttg acaaacatt ttgtcataa 
      tgcacacag tcatggcta ataatactt gtgaaggca 
      tggatgaag tcggactgc tccggtacg tcataatca 
      acctagtct tcttcatcg gggttgccg gcaacctgg 
      ccgccctgt ggatccttt acaaatcgg ccaagacca 
      gaaacaaaa agcacgttt tattgttaa ggtgcctag 
      cagtcaacg atttagtgg ctgaagttg gtatgtttt 
      ccattataa ctctacaaa aatacaagt tgattcccg 
      tgtattgga tttgtgttg gttttgtgt tacttcgtg 
      cttttggaa tcggatcgg gatgtgttg cttttgtga 
      tggccctcg agcgatggt tggccctta caagacctt 
      tctattatc aacagctag tcacttacc gaattttaa 
      aaatatttg tattctctt tgtggtccg gaggagcct 
      tattaactt accttcccc ttcttggtt tcggattgt 
      actacaacg gtaaatgtt gcagataca gagacgcaa 
      cagaacttc gagatcaaa tatatgctt acatgttct 
      tggttttcg gtactgcgt tatgcttgt gcatagcat 
      tttgcaatt taactgtta tatgggagt tatcaaaaa 
      tccgtccac atggccgtg ttttaatga ggagggtta 
      gcaggtcga caatgaaca gccgtgtta tggctgcaa 
      aatatcaaa cccccgaag aaatagctt ttgcaaaat 
      tgatggcaa ttatttgta tcatatttg tagtttgct 
      gggctcctc aaatgatct caattccat tagcccagt 
      tttggaaag gttcaactt ttgatggga tatttgcaa 
      aaatggcaa attttctca tgtgtactt actttactt 
      tggacccgt acgtttacg tgttgcagc gttacatcg 
      aaaaaaaga aaatagcgc tgggttgtt gctggccgc 
      gccgatcca cggaagaaa acgtcacat cgcttccgg 
      ccgttttgc aaagcgaac aagacgaaa agaatctgt 
      atgtagata gatacttat aaagtatag tatgtacac 
      atacgctag tataatgta tacttatta accaaagac 
      tcttgtgca tgactaatt tcaatggat attttatat 
      gttctttgt cgtcatatc tatttataa taagaaaca 
      ttgcaatat tttcacagt gtctggaga atatttaaa 
      aatactgta ttttgtgtg taagaacgt tcttataat 
      tattgtagt tttgttgtc tttattttg ttaatctac 
      acgtctgta ctttagcca ttctaactt ctatgcatt 
      tggctctga taggtggcc acctctgtg taggtataa 
      agacgtaac tgtttgtaa atatatggt aaactggtg 
      ctaacagat tgttagagg actagttga ctgtgtatg 
      taaaattgt ttttggaga caagaacac ggtattgta 
      tttttgtta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY