General information | |||
TC013650 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 7069125..7075142 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC013650 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05473 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
legs partiallynecrotic - Number of animals showing the phenotype: 3
Comment: Especially metathoracic legs are affected. |
metathoracic leg tibia not present - Number of animals showing the phenotype: 1
metathoracic leg tarsus not present - Number of animals showing the phenotype: 1
metathoracic leg pretarsus not present - Number of animals showing the phenotype: 1
- >TC013650 RA
MHTVMANNT CEGMDEVGL LRYVIINLV FFIGVAGNL AALWILYKS AKTRNKKHV LLLRCLAVN DLVAEVGMF SIITLQKYK LIPVYWICV GFVLLRAFG IGSGCVAFV MALERWLAL TRPFYYQQL VTYRILKIF VFSLWSGGA LLTYLPLLG FGLYYNGKC CRYRDATEL RDQIYAYMF LVFGTALCL CIAFCNLTV IWELSKIRP HGRVLMRRV SRSTMNSRV MAAKYQTPE EIAFAKLMA IICIIFVVC WAPQMISIP LAQFWKGST FDGIFAKMA NFLMCTYFT LDPYVYVLQ RYIEKKKIA LGCCWPRRS TGSITTSSI TAGTPTSVL LNPPSFTSP SIM
- >TC013650 RB
MHTVMANNT CEGMDEVGL LRYVIINLV FFIGVAGNL AALWILYKS AKTRNKKHV LLLRCLAVN DLVAEVGMF SIITLQKYK LIPVYWICV GFVLLRAFG IGSGCVAFV MALERWLAL TRPFYYQQL VTYRILKIF VFSLWSGGA LLTYLPLLG FGLYYNGKC CRYRDATEL RDQIYAYMF LVFGTALCL CIAFCNLTV IWELSKIRP HGRVLMRRV SRSTMNSRV MAAKYQTPE EIAFAKLMA IICIIFVVC WAPQMISIP LAQFWKGST FDGIFAKMA NFLMCTYFT LDPYVYVLQ RYIEKKKIA LGCCWPRRS TEENVTSLP AVLQSEQDE KNLYVDRYL
- >TC013650 RA
atgcacaca gtcatggct aataatact tgtgaaggc atggatgaa gtcggactg ctccggtac gtcataatc aacctagtc ttcttcatc ggggttgcc ggcaacctg gccgccctg tggatcctt tacaaatcg gccaagacc agaaacaaa aagcacgtt ttattgtta aggtgccta gcagtcaac gatttagtg gctgaagtt ggtatgttt tccattata actctacaa aaatacaag ttgattccc gtgtattgg atttgtgtt ggttttgtg ttacttcgt gcttttgga atcggatcg ggatgtgtt gcttttgtg atggccctc gagcgatgg ttggccctt acaagacct ttctattat caacagcta gtcacttac cgaatttta aaaatattt gtattctct ttgtggtcc ggaggagcc ttattaact taccttccc cttcttggt ttcggattg tactacaac ggtaaatgt tgcagatac agagacgca acagaactt cgagatcaa atatatgct tacatgttc ttggttttc ggtactgcg ttatgcttg tgcatagca ttttgcaat ttaactgtt atatgggag ttatcaaaa atccgtcca catggccgt gttttaatg aggagggtt agcaggtcg acaatgaac agccgtgtt atggctgca aaatatcaa acccccgaa gaaatagct tttgcaaaa ttgatggca attatttgt atcatattt gtagtttgc tgggctcct caaatgatc tcaattcca ttagcccag ttttggaaa ggttcaact tttgatggg atatttgca aaaatggca aattttctc atgtgtact tactttact ttggacccg tacgtttac gtgttgcag cgttacatc gaaaaaaag aaaatagcg ctgggttgt tgctggccg cgccgatcc acgggtagt atcaccact tcttcaata acagcagga acgcccact tccgtactt ctgaatcct ccttccttt actagtcct tccattatg taa
- >TC013650 RB
atgcacaca gtcatggct aataatact tgtgaaggc atggatgaa gtcggactg ctccggtac gtcataatc aacctagtc ttcttcatc ggggttgcc ggcaacctg gccgccctg tggatcctt tacaaatcg gccaagacc agaaacaaa aagcacgtt ttattgtta aggtgccta gcagtcaac gatttagtg gctgaagtt ggtatgttt tccattata actctacaa aaatacaag ttgattccc gtgtattgg atttgtgtt ggttttgtg ttacttcgt gcttttgga atcggatcg ggatgtgtt gcttttgtg atggccctc gagcgatgg ttggccctt acaagacct ttctattat caacagcta gtcacttac cgaatttta aaaatattt gtattctct ttgtggtcc ggaggagcc ttattaact taccttccc cttcttggt ttcggattg tactacaac ggtaaatgt tgcagatac agagacgca acagaactt cgagatcaa atatatgct tacatgttc ttggttttc ggtactgcg ttatgcttg tgcatagca ttttgcaat ttaactgtt atatgggag ttatcaaaa atccgtcca catggccgt gttttaatg aggagggtt agcaggtcg acaatgaac agccgtgtt atggctgca aaatatcaa acccccgaa gaaatagct tttgcaaaa ttgatggca attatttgt atcatattt gtagtttgc tgggctcct caaatgatc tcaattcca ttagcccag ttttggaaa ggttcaact tttgatggg atatttgca aaaatggca aattttctc atgtgtact tactttact ttggacccg tacgtttac gtgttgcag cgttacatc gaaaaaaag aaaatagcg ctgggttgt tgctggccg cgccgatcc acggaagaa aacgtcaca tcgcttccg gccgttttg caaagcgaa caagacgaa aagaatctg tatgtagat agatactta taa
- >TC013650 RA
ggagacctt aattatttg gggttttct caacgaaat tcatcaagt ggcctggca gtttccttt cgcccacca ccctttaaa gacgtctgg gcccaaagc agaagcagt aagaggtca gctgtgttg acaaacatt ttgtcataa tgcacacag tcatggcta ataatactt gtgaaggca tggatgaag tcggactgc tccggtacg tcataatca acctagtct tcttcatcg gggttgccg gcaacctgg ccgccctgt ggatccttt acaaatcgg ccaagacca gaaacaaaa agcacgttt tattgttaa ggtgcctag cagtcaacg atttagtgg ctgaagttg gtatgtttt ccattataa ctctacaaa aatacaagt tgattcccg tgtattgga tttgtgttg gttttgtgt tacttcgtg cttttggaa tcggatcgg gatgtgttg cttttgtga tggccctcg agcgatggt tggccctta caagacctt tctattatc aacagctag tcacttacc gaattttaa aaatatttg tattctctt tgtggtccg gaggagcct tattaactt accttcccc ttcttggtt tcggattgt actacaacg gtaaatgtt gcagataca gagacgcaa cagaacttc gagatcaaa tatatgctt acatgttct tggttttcg gtactgcgt tatgcttgt gcatagcat tttgcaatt taactgtta tatgggagt tatcaaaaa tccgtccac atggccgtg ttttaatga ggagggtta gcaggtcga caatgaaca gccgtgtta tggctgcaa aatatcaaa cccccgaag aaatagctt ttgcaaaat tgatggcaa ttatttgta tcatatttg tagtttgct gggctcctc aaatgatct caattccat tagcccagt tttggaaag gttcaactt ttgatggga tatttgcaa aaatggcaa attttctca tgtgtactt actttactt tggacccgt acgtttacg tgttgcagc gttacatcg aaaaaaaga aaatagcgc tgggttgtt gctggccgc gccgatcca cgggtagta tcaccactt cttcaataa cagcaggaa cgcccactt ccgtacttc tgaatcctc cttccttta ctagtcctt ccattatgt aaattttaa cctttttac aagtattta ttggtttct tcaagatat ttcttaatt ataccactt atctacgtt gtttagtac gtatatagg agtctttgt ttagaaaat tacttgacc caaatctcg cttgatgaa tgactcaga taagacagc attgcatgt tgattcaaa atttatcta ccagtaggg cagatggta ttacgaata cactttata aagcgaaca cttcaagga atatcgagt ggttttcca ggttgagtt aacaaaagt tgaaagcac aattttttg tgttcagaa tatatgaca aatgtgata gctaggagt cgtaacata agcagcgtt aaaacctgc tttgagatt ttaacatgg cttgacttg tgtattaaa acttctagt gtaaaataa aacgaaatt aacggagat tgttcttgg aattttctg atcatttca cttcataaa atatttgtt ttatcagta ccgacttct ccctttagt ccaaagcgt tttacttac actgcatgt attgtttaa ctaatttac atagttcgt gcgtttaaa gcgtttaca tatattcgt tatcaaagt taagcgaaa atgtatatc tgttgtttt ttttataga agaaaacgt cacatcgct tccggccgt tttgcaaag cgaacaaga cgaaaagaa tctgtatgt agatagata cttataaag tatagtatg tacacatac gctagtata atgtatact tattaacca aagactctt gtgcatgac taatttcaa tggatattt tatatgttc tttgtcgtc atatctatt tataataag aaacattgc aatattttc acagtgtct ggagaatat ttaaaaata ctgtatttt gtgtgtaag aacgttctt ataattatt gtagttttg ttgtcttta ttttgttaa tctacacgt ctgtacttt agccattct aacttctat gcatttggc tctgatagg tggccacct ctgtgtagg tataaagac gtaactgtt tgtaaatat atggtaaac tggtgctaa cagattgtt agaggacta gttgactgt gtatgtaaa attgttttt ggagacaag aacacggta ttgtatttt tgttat
- >TC013650 RB
ggagacctt aattatttg gggttttct caacgaaat tcatcaagt ggcctggca gtttccttt cgcccacca ccctttaaa gacgtctgg gcccaaagc agaagcagt aagaggtca gctgtgttg acaaacatt ttgtcataa tgcacacag tcatggcta ataatactt gtgaaggca tggatgaag tcggactgc tccggtacg tcataatca acctagtct tcttcatcg gggttgccg gcaacctgg ccgccctgt ggatccttt acaaatcgg ccaagacca gaaacaaaa agcacgttt tattgttaa ggtgcctag cagtcaacg atttagtgg ctgaagttg gtatgtttt ccattataa ctctacaaa aatacaagt tgattcccg tgtattgga tttgtgttg gttttgtgt tacttcgtg cttttggaa tcggatcgg gatgtgttg cttttgtga tggccctcg agcgatggt tggccctta caagacctt tctattatc aacagctag tcacttacc gaattttaa aaatatttg tattctctt tgtggtccg gaggagcct tattaactt accttcccc ttcttggtt tcggattgt actacaacg gtaaatgtt gcagataca gagacgcaa cagaacttc gagatcaaa tatatgctt acatgttct tggttttcg gtactgcgt tatgcttgt gcatagcat tttgcaatt taactgtta tatgggagt tatcaaaaa tccgtccac atggccgtg ttttaatga ggagggtta gcaggtcga caatgaaca gccgtgtta tggctgcaa aatatcaaa cccccgaag aaatagctt ttgcaaaat tgatggcaa ttatttgta tcatatttg tagtttgct gggctcctc aaatgatct caattccat tagcccagt tttggaaag gttcaactt ttgatggga tatttgcaa aaatggcaa attttctca tgtgtactt actttactt tggacccgt acgtttacg tgttgcagc gttacatcg aaaaaaaga aaatagcgc tgggttgtt gctggccgc gccgatcca cggaagaaa acgtcacat cgcttccgg ccgttttgc aaagcgaac aagacgaaa agaatctgt atgtagata gatacttat aaagtatag tatgtacac atacgctag tataatgta tacttatta accaaagac tcttgtgca tgactaatt tcaatggat attttatat gttctttgt cgtcatatc tatttataa taagaaaca ttgcaatat tttcacagt gtctggaga atatttaaa aatactgta ttttgtgtg taagaacgt tcttataat tattgtagt tttgttgtc tttattttg ttaatctac acgtctgta ctttagcca ttctaactt ctatgcatt tggctctga taggtggcc acctctgtg taggtataa agacgtaac tgtttgtaa atatatggt aaactggtg ctaacagat tgttagagg actagttga ctgtgtatg taaaattgt ttttggaga caagaacac ggtattgta tttttgtta t
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
