Status
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Gene information
General information
TC013635 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2791187..2795944
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013635
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05471

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    maxilla not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    prothoracic leg sometimes present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Appendages shape/orientation sometimes irregular.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Appendages shape/orientation sometimes irregular.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings slightlynot closed - Number of animals showing the phenotype: 5

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013635 RA
      MNKLVVLLF VVPLSAALN VVTLKYCCG VGTQHANAG 
      HQCEAFKPP IDGVRKDDE SSCLHSIDV CCKNRIQEI 
      QCDKGINDA KKGQSCNGD GFQKDCCEA CQLGRDTGT 
      STKACQELG LGLPFDKAF TECCKEIIE SPTTTTTTE 
      RTTTEAIKS TPSTIYSPL PPLENVCDI GDVCAQICI 
      PTQDSYKCA CNKGFTLMG DGISCKPDK NKSPKKNDR 
      CSVNNPCDH ECIDTGVAI KCSCREGYE LAKDKRTCQ 
      DIDECAQGI HQCEPTEEC VNEEGSYFC DDPNFQPET 
      DLNTDIELD GNCPTGYKF NYEKKVCDD IDECEFDII 
      CPRPQTCIN TIGSFKCED NTPQCPPGF HYKDSIETC 
      TDIDECMTG DNDCNKESQ VCLNTKGNY TCIDKVSKK 
      TCPPGFKKN PLTQNCDDI DECVENEEP CGFNEECVN 
      EPGGHNCVP RSGKKTYEY PLRPPYEPV LPPKPTEVA 
      SSTSTSTST STTTTTTTT KPHVSCPTG YRYSPQTNS 
      CLDIDECSE DPQTCDSSQ ECYNSPGSY ICKCKPGFS 
      KDFQTGACV DINECQLNQ HDCTQAQRC DNTIGSYLC 
      ARITGCGTG YTLNYANGL CEDDDECAL NTHTCNSLG 
      PNFRCRNTL GSYRCDPIR IVPKPAPTP LIPVFSTPF 
      STQNYPVLI GTRKKCLPG YIMNQHGVC EDINECESN 
      PCPRGQKCL NFNGRYECL SELQCKIGY EMDEAGENC 
      LDINECTRG THKCKDTQI CKNGRGFYT CECPPGHHM 
      NPMTQRCED IDECKHYRA CYLNAECVN TIGSYKCHC 
      KEGFRGKGN SCEDIDECK ENAGLCEHS CVNIWGSYR 
      CACKQGFIL SYDNRTCTD IDECQKFKD KRLCVGICE 
      NVPGSYRCK CPDGYRLGN DGRTCIDID ECQQNICPR 
      HDDICLNTR GGYKCYTIN CPPNYIRDP QHKGRCKLP 
      QNFCDRRDI QCILRPEQY TYHYITLVS KLPIQRDSV 
      QFFQINGPI WITTRAEFS MEVVKVEAP YGVQKCDER 
      YFKMVKLDP NAMKIFLVR SIEGPQEVQ LKVEMHLYQ 
      NNILTANVV SYIFIVVSA YPF
    CDS sequence
    • >TC013635 RA
      atgaacaaa ctcgtagtt ttgctattc gtagttccc 
      ctaagtgca gcattaaat gtcgtcaca cttaagtat 
      tgttgcgga gttggtacc cagcatgct aacgccggg 
      caccaatgc gaagctttc aagcccccg atagacggg 
      gtgcgaaaa gacgacgag tcctcctgt ctccattca 
      atcgacgtt tgttgcaaa aaccgaatt caggaaata 
      caatgcgac aaaggcata aatgacgct aagaaggga 
      caatcgtgt aacggggac ggtttccaa aaagactgt 
      tgcgaggct tgtcaattg ggacgcgac acaggcacg 
      tccacaaaa gcttgtcag gagttagga cttggcctc 
      ccgttcgat aaagctttt actgaatgt tgcaaagag 
      ataattgag tctccaaca acaacaaca acaactgaa 
      agaacaacc accgaggca atcaaatcg accccatcc 
      acaatttac tccccattg ccacctctg gaaaacgtg 
      tgtgatatt ggcgacgtt tgcgcccaa atctgcatc 
      ccgacccag gattcgtac aaatgtgcg tgcaacaaa 
      gggttcact ttaatgggc gatggcatt tcgtgcaag 
      cctgacaaa aataagtcg ccgaaaaag aatgataga 
      tgttccgtg aacaatccc tgcgaccac gagtgcatc 
      gacacaggg gtcgcaatt aagtgctcg tgtcgggag 
      ggctacgag ctagccaag gacaaaagg acgtgccaa 
      gatatcgac gagtgcgcc cagggaatc caccagtgc 
      gagcccacc gaagaatgc gtgaacgaa gaagggtcg 
      tacttctgc gacgacccg aacttccag cccgagacc 
      gacctgaac accgacatc gaactggac ggcaactgc 
      cccactggc tacaaattc aattacgag aaaaaagtc 
      tgcgatgac attgatgaa tgcgagttt gatatcatt 
      tgcccgcgg ccgcagact tgcatcaac acaattggc 
      tcgtttaaa tgcgaggat aacaccccg caatgcccc 
      ccgggattt cactacaag gactccatc gagacgtgc 
      acagacatc gacgaatgt atgaccgga gacaacgat 
      tgtaacaaa gagtcgcaa gtttgcctc aacacgaaa 
      gggaactac acttgcatc gataaagtt tcgaaaaag 
      acgtgccct cccgggttc aagaaaaac ccgcttacg 
      caaaactgc gacgacatt gacgagtgc gtcgaaaac 
      gaagaaccg tgcgggttt aacgaagag tgtgtcaat 
      gaacctggg gggcacaac tgtgttcca agaagtggg 
      aaaaagacg tacgagtac ccactgcgg cccccctac 
      gagccggta ctgcccccg aaacccacc gaggtggcc 
      tccagtacc tccactagc actagcact agtaccacc 
      accaccacg actacgaca aagccacac gtttcgtgc 
      ccaactggg tacagatat tccccacaa accaactca 
      tgccttgat atcgacgaa tgttccgaa gatccacaa 
      acctgcgac agcagtcag gaatgctac aacagtcct 
      gggagctac atttgtaaa tgcaagccc gggtttagt 
      aaagacttc caaaccgga gcttgcgtc gatataaat 
      gagtgccag ttaaaccag cacgactgc acgcaggcc 
      cagcgctgc gacaacacg atcggttct tacctttgt 
      gccaggata actggctgt ggcactggc tatacctta 
      aactatgcc aatggttta tgcgaagac gacgacgag 
      tgtgcctta aatacgcac acgtgtaac agtttaggt 
      ccaaatttc aggtgccgg aacactttg ggttcctac 
      cgttgtgac cctatcaga attgtacca aaaccagcg 
      cctacccct ttaatccct gttttctcg acacctttc 
      agcactcaa aattacccc gttctgatc gggacacgt 
      aaaaaatgc cttccgggc tacataatg aaccaacac 
      ggtgtctgt gaagatata aacgagtgc gaaagcaat 
      ccctgtccc cggggccaa aagtgcctc aatttcaat 
      ggtaggtat gagtgtctt tcggagcta cagtgcaaa 
      attggctac gagatggac gaagcaggg gaaaattgt 
      ctcgatata aatgagtgt acgagaggt acgcacaaa 
      tgcaaggac acccaaatt tgcaaaaac ggacgcgga 
      ttttacacg tgtgagtgc cctccgggc caccacatg 
      aaccccatg actcagaga tgcgaagat atcgacgaa 
      tgcaaacat tacagggct tgctatctt aatgccgaa 
      tgtgtgaat acaatcggc tcgtacaaa tgccactgt 
      aaggaggga tttcgggga aagggcaac tcgtgtgag 
      gacattgac gaatgcaaa gaaaatgca gggttgtgt 
      gaacacagc tgtgtcaac atttggggt tcttatcga 
      tgcgcttgc aagcaaggt tttatatta agttacgac 
      aacagaacg tgtacggac atcgacgaa tgccagaaa 
      ttcaaagat aagagactt tgtgttgga atatgtgaa 
      aatgttccg gggagttac cggtgcaaa tgtccggac 
      gggtatcgt ttagggaac gatgggcga acgtgcatc 
      gatattgac gaatgccag caaaacatc tgtcctcgc 
      cacgatgac atttgtttg aacacccgg ggcggctat 
      aaatgctac acaatcaac tgtcccccg aactacatc 
      agggacccc caacacaaa gggcgctgt aaactgccg 
      caaaatttc tgcgaccgc agagacatc cagtgtatt 
      ttaaggccc gagcagtac acttatcac tacataacg 
      ctcgtttcc aaactacca atccaacgc gacagcgtc 
      caattcttc caaatcaac ggcccgata tggatcacc 
      acccgcgcc gagttctcg atggaagtg gtcaaagtc 
      gaggcaccg tacggtgtg caaaaatgc gacgagcgc 
      tatttcaaa atggtcaag cttgacccc aacgctatg 
      aaaatattc ctggtgcga tcgattgaa ggcccccag 
      gaagtccag ctcaaagtc gagatgcac ctctaccaa 
      aacaacata ctaacagcg aacgtggtc tcgtacatt 
      tttatcgta gtttcggct tatcccttc tag
    mRNA sequence
    • >TC013635 RA
      aaagcatta tctgcggct ggttggaag agggaaggt 
      agatttgtc cgtaacaat atttgtttg gaaagataa 
      gtaaatgca tcttgtgcc cttaacaga gaatgaagt 
      aaataaacg tttgataac agtgatcag tgcagttcg 
      ggtttacac aagtgcgaa atatgaaca aactcgtag 
      ttttgctat tcgtagttc ccctaagtg cagcattaa 
      atgtcgtca cacttaagt attgttgcg gagttggta 
      cccagcatg ctaacgccg ggcaccaat gcgaagctt 
      tcaagcccc cgatagacg gggtgcgaa aagacgacg 
      agtcctcct gtctccatt caatcgacg tttgttgca 
      aaaaccgaa ttcaggaaa tacaatgcg acaaaggca 
      taaatgacg ctaagaagg gacaatcgt gtaacgggg 
      acggtttcc aaaaagact gttgcgagg cttgtcaat 
      tgggacgcg acacaggca cgtccacaa aagcttgtc 
      aggagttag gacttggcc tcccgttcg ataaagctt 
      ttactgaat gttgcaaag agataattg agtctccaa 
      caacaacaa caacaactg aaagaacaa ccaccgagg 
      caatcaaat cgaccccat ccacaattt actccccat 
      tgccacctc tggaaaacg tgtgtgata ttggcgacg 
      tttgcgccc aaatctgca tcccgaccc aggattcgt 
      acaaatgtg cgtgcaaca aagggttca ctttaatgg 
      gcgatggca tttcgtgca agcctgaca aaaataagt 
      cgccgaaaa agaatgata gatgttccg tgaacaatc 
      cctgcgacc acgagtgca tcgacacag gggtcgcaa 
      ttaagtgct cgtgtcggg agggctacg agctagcca 
      aggacaaaa ggacgtgcc aagatatcg acgagtgcg 
      cccagggaa tccaccagt gcgagccca ccgaagaat 
      gcgtgaacg aagaagggt cgtacttct gcgacgacc 
      cgaacttcc agcccgaga ccgacctga acaccgaca 
      tcgaactgg acggcaact gccccactg gctacaaat 
      tcaattacg agaaaaaag tctgcgatg acattgatg 
      aatgcgagt ttgatatca tttgcccgc ggccgcaga 
      cttgcatca acacaattg gctcgttta aatgcgagg 
      ataacaccc cgcaatgcc ccccgggat ttcactaca 
      aggactcca tcgagacgt gcacagaca tcgacgaat 
      gtatgaccg gagacaacg attgtaaca aagagtcgc 
      aagtttgcc tcaacacga aagggaact acacttgca 
      tcgataaag tttcgaaaa agacgtgcc ctcccgggt 
      tcaagaaaa acccgctta cgcaaaact gcgacgaca 
      ttgacgagt gcgtcgaaa acgaagaac cgtgcgggt 
      ttaacgaag agtgtgtca atgaacctg gggggcaca 
      actgtgttc caagaagtg ggaaaaaga cgtacgagt 
      acccactgc ggcccccct acgagccgg tactgcccc 
      cgaaaccca ccgaggtgg cctccagta cctccacta 
      gcactagca ctagtacca ccaccacca cgactacga 
      caaagccac acgtttcgt gcccaactg ggtacagat 
      attccccac aaaccaact catgccttg atatcgacg 
      aatgttccg aagatccac aaacctgcg acagcagtc 
      aggaatgct acaacagtc ctgggagct acatttgta 
      aatgcaagc ccgggttta gtaaagact tccaaaccg 
      gagcttgcg tcgatataa atgagtgcc agttaaacc 
      agcacgact gcacgcagg cccagcgct gcgacaaca 
      cgatcggtt cttaccttt gtgccagga taactggct 
      gtggcactg gctatacct taaactatg ccaatggtt 
      tatgcgaag acgacgacg agtgtgcct taaatacgc 
      acacgtgta acagtttag gtccaaatt tcaggtgcc 
      ggaacactt tgggttcct accgttgtg accctatca 
      gaattgtac caaaaccag cgcctaccc ctttaatcc 
      ctgttttct cgacacctt tcagcactc aaaattacc 
      ccgttctga tcgggacac gtaaaaaat gccttccgg 
      gctacataa tgaaccaac acggtgtct gtgaagata 
      taaacgagt gcgaaagca atccctgtc cccggggcc 
      aaaagtgcc tcaatttca atggtaggt atgagtgtc 
      tttcggagc tacagtgca aaattggct acgagatgg 
      acgaagcag gggaaaatt gtctcgata taaatgagt 
      gtacgagag gtacgcaca aatgcaagg acacccaaa 
      tttgcaaaa acggacgcg gattttaca cgtgtgagt 
      gccctccgg gccaccaca tgaacccca tgactcaga 
      gatgcgaag atatcgacg aatgcaaac attacaggg 
      cttgctatc ttaatgccg aatgtgtga atacaatcg 
      gctcgtaca aatgccact gtaaggagg gatttcggg 
      gaaagggca actcgtgtg aggacattg acgaatgca 
      aagaaaatg cagggttgt gtgaacaca gctgtgtca 
      acatttggg gttcttatc gatgcgctt gcaagcaag 
      gttttatat taagttacg acaacagaa cgtgtacgg 
      acatcgacg aatgccaga aattcaaag ataagagac 
      tttgtgttg gaatatgtg aaaatgttc cggggagtt 
      accggtgca aatgtccgg acgggtatc gtttaggga 
      acgatgggc gaacgtgca tcgatattg acgaatgcc 
      agcaaaaca tctgtcctc gccacgatg acatttgtt 
      tgaacaccc ggggcggct ataaatgct acacaatca 
      actgtcccc cgaactaca tcagggacc cccaacaca 
      aagggcgct gtaaactgc cgcaaaatt tctgcgacc 
      gcagagaca tccagtgta ttttaaggc ccgagcagt 
      acacttatc actacataa cgctcgttt ccaaactac 
      caatccaac gcgacagcg tccaattct tccaaatca 
      acggcccga tatggatca ccacccgcg ccgagttct 
      cgatggaag tggtcaaag tcgaggcac cgtacggtg 
      tgcaaaaat gcgacgagc gctatttca aaatggtca 
      agcttgacc ccaacgcta tgaaaatat tcctggtgc 
      gatcgattg aaggccccc aggaagtcc agctcaaag 
      tcgagatgc acctctacc aaaacaaca tactaacag 
      cgaacgtgg tctcgtaca tttttatcg tagtttcgg 
      cttatccct tctagaata gcgaggtgg aggatgtgc 
      gagttttca gcggtgtta gtctagcta gtttgtacg 
      agtact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY