Status
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Gene information
General information
TC031494 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2818575..2823578
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031494
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05469

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031494 RA
      MFLLCLLGC LALPVYAQG PQYDNAGRP YNAQNSVYD 
      QNVPYAQQG PQYQQPPQG PLYNQDPQY NPQPNIPRI 
      YSNDGLNND IFYDLKCPE HWVRLYQSC YRFIRSPLR 
      PYEEARRIC EAYSPEDGR SDLVSIGST EEHGFLINQ 
      LNALDPQHR RWYIGTYQH SANYWTNVD GSQLVNMEN 
      AFLPVAEPY GKDHLAYNF SVELMHWGF QPXXXXXXX 
      XXXXXXXXX XXXXXXRTY TYGVEITNP EQIPRGPYF 
      IKQPVDATF DSSKKMLYN DISLSCLAG GFPTPTYEW 
      FREDYEIDK LVARRIDPL SDPRYTISG GMLIIHEPE 
      EKRDHATYH CKASNKFGT IISESVQLN FGFIKEFVL 
      KRSPESGDK NWGKAIYCD PPNHFPSVR YSWSRDYFP 
      NFVEEDRRV FVSYDGALY FSALETIDK ANYSCSVQS 
      EFSDSGRNG PFFPLMVNP HSHYQQLKF PNNFPKAFP 
      DAPIAGKEV RLECMAFGY PVPSYNWTR RGAPLPRSA 
      VLSSFNRVL TIPHVQVED QGEYVCRAY NDRASTENS 
      LILNIQAEP NFTIPLADR HADSKSELV WTCEAFGIP 
      DVNYTWWKN GRQLVMGYM EPEERDRIR IQDNVLTIR 
      YLDQERDPG MYQCRATNS LKTKYSSAQ LRVLAFRPT 
      FKKHPLEAE TYAAEGGSV TIKCNPEAA PRPEFVWKK 
      DGNVLGSGG HRRILENGN LVISSVSRD DEGQYTCRA 
      SNQFGVDES KGRLIVLRG PRLIESLTP KIVTTVGRY 
      FDLRCLAET EEMLDIAYV WSHNGIKIR DIDIKNMNY 
      RLRIDGGFL SVTNATFTD AGEYECTIK STVGKISSR 
      SVVVVEGPP GPPGGVTVS IQKSSFTLQ WTDGATHGS 
      QIRGYTVSG RTNWNQTWV NISYIVDVR EIDRYTGQK 
      ESLIDTALT PWSTYEFRI AAWNSWGMG PPSAPSPRH 
      STPPDRPYI APYNIGGGG GKIGDLTIT WSPLKPDEQ 
      NGPGIYYRI FWKRKDQEV EFQTQELKE FGNTGMAVV 
      HIPPQYYYT EYIVKVQAI NQIGPGPIS PEHVIYSAE 
      DMPQVAPQL VVARSFNST ALNVSWVPI DQTREKVRG 
      KLIGHLVWR YVQPSLEEE PLKGYKIRV WEFDQDIST 
      ANDTIKPFG GKLEAYVNN LQPGKTYRM RVLAYSNGG 
      DGRMSSPEV KFRMGGFSN SPLAYYRSA CEDLKPFTI 
      TIVIACLLH YLLTT
    • >TC031494 RB
      MFLLCLLGC LALPVYAQG PQYDNAGRP YNAQNSVYD 
      QNVPYAQQG PQYQQPPQG PLYNQDPQY NPQPNIPRI 
      YSNDGLNND IFYDLKCPE HWVRLYQSC YRFIRSPLR 
      PYEEARRIC EAYSPEDGR SDLVSIGST EEHGFLINQ 
      LNALDPQHR RWYIGTYQH SANYWTNVD GSQLVNMEN 
      AFLPVAEPY GKDHLAYNF SVELMHWGF QPXXXXXXX 
      XXXXXXXXX XXXXXXRTY TYGVEITNP EQIPRGPYF 
      IKQPVDATF DSSKKMLYN DISLSCLAG GFPTPTYEW 
      FREDYEIDK LVARRIDPL SDPRYTISG GMLIIHEPE 
      EKRDHATYH CKASNKFGT IISESVQLN FGFIKEFVL 
      KRSPESGDK NWGKAIYCD PPNHFPSVR YSWSRDYFP 
      NFVEEDRRV FVSYDGALY FSALETIDK ANYSCSVQS 
      EFSDSGRNG PFFPLMVNP HSHYQQLKF PNNFPKAFP 
      DAPIAGKEV RLECMAFGY PVPSYNWTR RGAPLPRSA 
      VLSSFNRVL TIPHVQVED QGEYVCRAY NDRASTENS 
      LILNIQAEP NFTIPLADR HADSKSELV WTCEAFGIP 
      DVNYTWWKN GRQLVMGYM EPEERDRIR IQDNVLTIR 
      YLDQERDPG MYQCRATNS LKTKYSSAQ LRVLAFRPT 
      FKKHPLEAE TYAAEGGSV TIKCNPEAA PRPEFVWKK 
      DGNVLGSGG HRRILENGN LVISSVSRD DEGQYTCRA 
      SNQFGVDES KGRLIVLRG PRLIESLTP KIVTTVGRY 
      FDLRCLAET EEMLDIAYV WSHNGIKIR DIDIKNMNY 
      RLRIDGGFL SVTNATFTD AGEYECTIK STVGKISSR 
      SVVVVEGPP GPPGGVTVS IQKSSFTLQ WTDGATHGS 
      QIRGYTVSG RTNWNQTWV NISYIVDVR EIDRYTGQK 
      ESLIDTALT PWSTYEFRI AAWNSWGMG PPSAPSPRH 
      STPPDRPYI APYNIGGGG GKIGDLTIT WSPLKPDEQ 
      NGPGIYYRI FWKRKDQEV EFQTQELKE FGNTGMAVV 
      HIPPQYYYT EYIVKVQAI NQIGPGPIS PEHVIYSAE 
      DMPQVAPQL VVARSFNST ALNVSWVPI DQTREKVRG 
      KLIGHLVWR YVQPSLEEE PLKGYKIRV WEFDQDIST 
      ANDTIKPFG GKLEAYVNN LQPGKTYRM RVLAYSNGG 
      DGRMSSPEV KFRMGPLAY YRSACEDLK PFTITIVIA 
      CLLHYLLTT
    CDS sequence
    • >TC031494 RA
      atgttcttg ctgtgtctt ctcgggtgc cttgcactc 
      ccggtttat gcacaagga ccccaatat gacaatgct 
      gggcgaccg tacaatgca caaaactca gtttacgac 
      caaaatgta ccttacgcc caacagggg ccacaatat 
      cagcagccc cctcaaggg cccttgtac aaccaggac 
      ccccagtac aatccgcag ccaaatatt ccaagaatt 
      tacagtaat gacggcctc aataacgat attttctac 
      gatttgaag tgcccagaa cactgggtt aggttgtat 
      cagtcgtgc tataggttt attaggtcg ccgttaagg 
      ccctatgag gaggcaaga cggatatgt gaagcctac 
      tctccggaa gatggcagg tcagatcta gtctcaatt 
      ggtagtacg gaagaacat ggcttcttg atcaatcag 
      ttgaacgct ttggaccct caacacagg aggtggtat 
      atagggacg taccaacac tcagcgaac tattggacc 
      aatgttgac ggttcccaa ttagtgaat atggaaaat 
      gcgtttttg ccggtcgcg gaaccctac ggaaaagac 
      cacctcgcg tataacttt tctgtcgag ctgatgcat 
      tggggcttc cagcccnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnc aggacttat acctatggt gtcgaaatc 
      accaacccc gaacagatc ccgaggggc ccctatttc 
      atcaaacaa ccagtcgac gccacgttc gatagctcg 
      aaaaaaatg ctatataac gatatcagt ctgagttgt 
      ttagccggt ggtttcccc accccgaca tacgagtgg 
      ttccgcgaa gactacgaa atcgacaaa ctcgtcgcc 
      cgtcgaata gaccccttg agtgacccc cgctacaca 
      atcagtggc gggatgttg ataatccac gaaccggag 
      gagaaacgt gaccatgcg acataccac tgcaaggct 
      tctaacaaa ttcggaact atcatttcc gaaagcgta 
      caactcaat ttcggtttt atcaaagaa ttcgtcctg 
      aaacgctcc cccgagtcg ggcgacaaa aactggggg 
      aaggccata tactgcgat cccccgaac cacttccct 
      tcggttcgc tactcgtgg tcacgtgac tacttcccg 
      aactttgtc gaggaagac cggcgcgtt ttcgtatcg 
      tatgacggc gccttgtac ttttcggcg ctagaaact 
      atagataaa gccaactac agttgtagt gtccagtcg 
      gagttttcc gattcgggc cggaacggc ccctttttc 
      ccactgatg gtcaaccct cattcccat taccaacaa 
      ctcaagttt ccgaataat ttccccaaa gctttcccc 
      gacgcgccc attgcgggg aaggaagta cgtctagag 
      tgtatggcg tttggatat cctgtccct agctacaac 
      tggacaaga cgcggggca cctttgcca cgatctgcc 
      gtcttgtct agtttcaac agggtgttg acaatccct 
      catgtgcag gtggaggat cagggagag tatgtttgc 
      cgggcttat aacgacaga gctagcacc gaaaatagc 
      cttatttta aatatacaa gccgaacct aacttcact 
      attccattg gctgacaga cacgccgat agtaaaagc 
      gagttagtt tggacttgt gaggcgttc ggaatccct 
      gatgttaac tacacgtgg tggaaaaac ggacggcag 
      ctagtcatg ggttatatg gaacccgaa gaacgcgac 
      cgaatccga atccaagac aacgtattg acgataagg 
      tacctggac caagaacgc gatcctgga atgtatcag 
      tgccgagcg acaaactcg ctcaagaca aaatactca 
      tccgcgcag ctgcgcgtt ttagctttc agacccact 
      tttaagaaa caccccctc gaagcggaa acttacgcc 
      gctgaaggg ggcagtgtg actataaaa tgtaaccca 
      gaggcggcc ccaaggccc gagttcgtg tggaagaaa 
      gacgggaat gttttaggt tcggggggg catcgaaga 
      attttagag aacggtaat ttggttatc agttcggtg 
      tcccgcgat gacgagggg cagtacacg tgcagggca 
      tctaatcag ttcggagtg gacgaatcg aagggacgc 
      ttgatcgtt ttacggggc ccgcgtttg attgaaagc 
      ttgactccg aaaattgtg acgacggtc ggccgttac 
      ttcgactta cgatgtctc gccgagact gaggaaatg 
      ttagacatt gcgtacgtt tggagccat aatggcatt 
      aaaatccgc gacatcgac atcaaaaac atgaattat 
      cgtttgcga atcgatggt gggtttctt tccgtgaca 
      aatgccacg tttaccgac gctggggaa tacgagtgt 
      actataaaa tcaaccgtt ggtaaaatc tcatctcgg 
      tcggtggtc gtggtcgag gggcctcct ggtccgcca 
      ggtggcgtc accgtgtcc attcagaag agctcgttc 
      acgctgcaa tggaccgat ggtgccact cacggctcc 
      caaatccgg ggttacacc gtcagtggt cgcacgaac 
      tggaaccag acctgggtc aacattagt tacattgtt 
      gatgtgcgg gagattgac agatacacc ggccagaag 
      gagagtctg attgacact gccttgact ccttggtcc 
      acttacgag tttcgcata gccgcttgg aactcctgg 
      gggatgggg cccccgtca gcccccagc ccccggcat 
      tcgacgccg ccggaccgt ccctacatc gccccatat 
      aacattggc ggtggaggc ggcaagatt ggcgatttg 
      accatcacg tggtcgcca ctcaagcca gacgagcag 
      aacggtcct ggcatttac tacaggata ttctggaag 
      cggaaggac caagaagtc gaattccag actcaggaa 
      ttgaaggag ttcgggaac acgggaatg gcggtagtg 
      cacataccg ccacagtac tactacacg gaatacatt 
      gttaaagtg caagctatt aatcagata ggtcctggg 
      ccgatcagt ccagaacat gttatatac tctgccgag 
      gacatgccg caagttgcg ccacaactg gtcgttgct 
      agatcgttc aactcgacg gcgcttaat gtcagttgg 
      gtaccaatt gaccagacg agggagaaa gtcagaggc 
      aaactcatc ggacatctg gtgtggcgg tacgtccaa 
      ccatcgttg gaggaagag ccgctcaaa ggctacaag 
      attcgtgta tgggaattc gatcaagac ataagcacc 
      gcgaatgac actataaaa ccattcggt ggcaaatta 
      gaagcctac gttaataac ctacagcct ggcaaaacc 
      tacagaatg cgtgtgctc gcatactcg aacggcggc 
      gacggccgc atgagctcc cccgaagtc aagttccga 
      atgggtggg tttagcaat agtccactt gcatattac 
      cgaagtgct tgtgaggat ttaaaacca tttacaata 
      acaatcgtc atcgcatgt ctgcttcat tatctacta 
      acaacatga
    • >TC031494 RB
      atgttcttg ctgtgtctt ctcgggtgc cttgcactc 
      ccggtttat gcacaagga ccccaatat gacaatgct 
      gggcgaccg tacaatgca caaaactca gtttacgac 
      caaaatgta ccttacgcc caacagggg ccacaatat 
      cagcagccc cctcaaggg cccttgtac aaccaggac 
      ccccagtac aatccgcag ccaaatatt ccaagaatt 
      tacagtaat gacggcctc aataacgat attttctac 
      gatttgaag tgcccagaa cactgggtt aggttgtat 
      cagtcgtgc tataggttt attaggtcg ccgttaagg 
      ccctatgag gaggcaaga cggatatgt gaagcctac 
      tctccggaa gatggcagg tcagatcta gtctcaatt 
      ggtagtacg gaagaacat ggcttcttg atcaatcag 
      ttgaacgct ttggaccct caacacagg aggtggtat 
      atagggacg taccaacac tcagcgaac tattggacc 
      aatgttgac ggttcccaa ttagtgaat atggaaaat 
      gcgtttttg ccggtcgcg gaaccctac ggaaaagac 
      cacctcgcg tataacttt tctgtcgag ctgatgcat 
      tggggcttc cagcccnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnc aggacttat acctatggt gtcgaaatc 
      accaacccc gaacagatc ccgaggggc ccctatttc 
      atcaaacaa ccagtcgac gccacgttc gatagctcg 
      aaaaaaatg ctatataac gatatcagt ctgagttgt 
      ttagccggt ggtttcccc accccgaca tacgagtgg 
      ttccgcgaa gactacgaa atcgacaaa ctcgtcgcc 
      cgtcgaata gaccccttg agtgacccc cgctacaca 
      atcagtggc gggatgttg ataatccac gaaccggag 
      gagaaacgt gaccatgcg acataccac tgcaaggct 
      tctaacaaa ttcggaact atcatttcc gaaagcgta 
      caactcaat ttcggtttt atcaaagaa ttcgtcctg 
      aaacgctcc cccgagtcg ggcgacaaa aactggggg 
      aaggccata tactgcgat cccccgaac cacttccct 
      tcggttcgc tactcgtgg tcacgtgac tacttcccg 
      aactttgtc gaggaagac cggcgcgtt ttcgtatcg 
      tatgacggc gccttgtac ttttcggcg ctagaaact 
      atagataaa gccaactac agttgtagt gtccagtcg 
      gagttttcc gattcgggc cggaacggc ccctttttc 
      ccactgatg gtcaaccct cattcccat taccaacaa 
      ctcaagttt ccgaataat ttccccaaa gctttcccc 
      gacgcgccc attgcgggg aaggaagta cgtctagag 
      tgtatggcg tttggatat cctgtccct agctacaac 
      tggacaaga cgcggggca cctttgcca cgatctgcc 
      gtcttgtct agtttcaac agggtgttg acaatccct 
      catgtgcag gtggaggat cagggagag tatgtttgc 
      cgggcttat aacgacaga gctagcacc gaaaatagc 
      cttatttta aatatacaa gccgaacct aacttcact 
      attccattg gctgacaga cacgccgat agtaaaagc 
      gagttagtt tggacttgt gaggcgttc ggaatccct 
      gatgttaac tacacgtgg tggaaaaac ggacggcag 
      ctagtcatg ggttatatg gaacccgaa gaacgcgac 
      cgaatccga atccaagac aacgtattg acgataagg 
      tacctggac caagaacgc gatcctgga atgtatcag 
      tgccgagcg acaaactcg ctcaagaca aaatactca 
      tccgcgcag ctgcgcgtt ttagctttc agacccact 
      tttaagaaa caccccctc gaagcggaa acttacgcc 
      gctgaaggg ggcagtgtg actataaaa tgtaaccca 
      gaggcggcc ccaaggccc gagttcgtg tggaagaaa 
      gacgggaat gttttaggt tcggggggg catcgaaga 
      attttagag aacggtaat ttggttatc agttcggtg 
      tcccgcgat gacgagggg cagtacacg tgcagggca 
      tctaatcag ttcggagtg gacgaatcg aagggacgc 
      ttgatcgtt ttacggggc ccgcgtttg attgaaagc 
      ttgactccg aaaattgtg acgacggtc ggccgttac 
      ttcgactta cgatgtctc gccgagact gaggaaatg 
      ttagacatt gcgtacgtt tggagccat aatggcatt 
      aaaatccgc gacatcgac atcaaaaac atgaattat 
      cgtttgcga atcgatggt gggtttctt tccgtgaca 
      aatgccacg tttaccgac gctggggaa tacgagtgt 
      actataaaa tcaaccgtt ggtaaaatc tcatctcgg 
      tcggtggtc gtggtcgag gggcctcct ggtccgcca 
      ggtggcgtc accgtgtcc attcagaag agctcgttc 
      acgctgcaa tggaccgat ggtgccact cacggctcc 
      caaatccgg ggttacacc gtcagtggt cgcacgaac 
      tggaaccag acctgggtc aacattagt tacattgtt 
      gatgtgcgg gagattgac agatacacc ggccagaag 
      gagagtctg attgacact gccttgact ccttggtcc 
      acttacgag tttcgcata gccgcttgg aactcctgg 
      gggatgggg cccccgtca gcccccagc ccccggcat 
      tcgacgccg ccggaccgt ccctacatc gccccatat 
      aacattggc ggtggaggc ggcaagatt ggcgatttg 
      accatcacg tggtcgcca ctcaagcca gacgagcag 
      aacggtcct ggcatttac tacaggata ttctggaag 
      cggaaggac caagaagtc gaattccag actcaggaa 
      ttgaaggag ttcgggaac acgggaatg gcggtagtg 
      cacataccg ccacagtac tactacacg gaatacatt 
      gttaaagtg caagctatt aatcagata ggtcctggg 
      ccgatcagt ccagaacat gttatatac tctgccgag 
      gacatgccg caagttgcg ccacaactg gtcgttgct 
      agatcgttc aactcgacg gcgcttaat gtcagttgg 
      gtaccaatt gaccagacg agggagaaa gtcagaggc 
      aaactcatc ggacatctg gtgtggcgg tacgtccaa 
      ccatcgttg gaggaagag ccgctcaaa ggctacaag 
      attcgtgta tgggaattc gatcaagac ataagcacc 
      gcgaatgac actataaaa ccattcggt ggcaaatta 
      gaagcctac gttaataac ctacagcct ggcaaaacc 
      tacagaatg cgtgtgctc gcatactcg aacggcggc 
      gacggccgc atgagctcc cccgaagtc aagttccga 
      atgggtcca cttgcatat taccgaagt gcttgtgag 
      gatttaaaa ccatttaca ataacaatc gtcatcgca 
      tgtctgctt cattatcta ctaacaaca tga
    mRNA sequence
    • >TC031494 RA
      aagatgact gtttagagt gagtgattc aattctaaa 
      acggttctc ctggttttt gttatcttt attatctct 
      tttctatga ttcggattt tatgagttg gtattatgt 
      tcttgctgt gtcttctcg ggtgccttg cactcccgg 
      tttatgcac aaggacccc aatatgaca atgctgggc 
      gaccgtaca atgcacaaa actcagttt acgaccaaa 
      atgtacctt acgcccaac aggggccac aatatcagc 
      agccccctc aagggccct tgtacaacc aggaccccc 
      agtacaatc cgcagccaa atattccaa gaatttaca 
      gtaatgacg gcctcaata acgatattt tctacgatt 
      tgaagtgcc cagaacact gggttaggt tgtatcagt 
      cgtgctata ggtttatta ggtcgccgt taaggccct 
      atgaggagg caagacgga tatgtgaag cctactctc 
      cggaagatg gcaggtcag atctagtct caattggta 
      gtacggaag aacatggct tcttgatca atcagttga 
      acgctttgg accctcaac acaggaggt ggtatatag 
      ggacgtacc aacactcag cgaactatt ggaccaatg 
      ttgacggtt cccaattag tgaatatgg aaaatgcgt 
      ttttgccgg tcgcggaac cctacggaa aagaccacc 
      tcgcgtata acttttctg tcgagctga tgcattggg 
      gcttccagc ccnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnncagga cttatacct atggtgtcg aaatcacca 
      accccgaac agatcccga ggggcccct atttcatca 
      aacaaccag tcgacgcca cgttcgata gctcgaaaa 
      aaatgctat ataacgata tcagtctga gttgtttag 
      ccggtggtt tccccaccc cgacatacg agtggttcc 
      gcgaagact acgaaatcg acaaactcg tcgcccgtc 
      gaatagacc ccttgagtg acccccgct acacaatca 
      gtggcggga tgttgataa tccacgaac cggaggaga 
      aacgtgacc atgcgacat accactgca aggcttcta 
      acaaattcg gaactatca tttccgaaa gcgtacaac 
      tcaatttcg gttttatca aagaattcg tcctgaaac 
      gctcccccg agtcgggcg acaaaaact gggggaagg 
      ccatatact gcgatcccc cgaaccact tcccttcgg 
      ttcgctact cgtggtcac gtgactact tcccgaact 
      ttgtcgagg aagaccggc gcgttttcg tatcgtatg 
      acggcgcct tgtactttt cggcgctag aaactatag 
      ataaagcca actacagtt gtagtgtcc agtcggagt 
      tttccgatt cgggccgga acggcccct ttttcccac 
      tgatggtca accctcatt cccattacc aacaactca 
      agtttccga ataatttcc ccaaagctt tccccgacg 
      cgcccattg cggggaagg aagtacgtc tagagtgta 
      tggcgtttg gatatcctg tccctagct acaactgga 
      caagacgcg gggcacctt tgccacgat ctgccgtct 
      tgtctagtt tcaacaggg tgttgacaa tccctcatg 
      tgcaggtgg aggatcagg gagagtatg tttgccggg 
      cttataacg acagagcta gcaccgaaa atagcctta 
      ttttaaata tacaagccg aacctaact tcactattc 
      cattggctg acagacacg ccgatagta aaagcgagt 
      tagtttgga cttgtgagg cgttcggaa tccctgatg 
      ttaactaca cgtggtgga aaaacggac ggcagctag 
      tcatgggtt atatggaac ccgaagaac gcgaccgaa 
      tccgaatcc aagacaacg tattgacga taaggtacc 
      tggaccaag aacgcgatc ctggaatgt atcagtgcc 
      gagcgacaa actcgctca agacaaaat actcatccg 
      cgcagctgc gcgttttag ctttcagac ccactttta 
      agaaacacc ccctcgaag cggaaactt acgccgctg 
      aagggggca gtgtgacta taaaatgta acccagagg 
      cggccccaa ggcccgagt tcgtgtgga agaaagacg 
      ggaatgttt taggttcgg gggggcatc gaagaattt 
      tagagaacg gtaatttgg ttatcagtt cggtgtccc 
      gcgatgacg aggggcagt acacgtgca gggcatcta 
      atcagttcg gagtggacg aatcgaagg gacgcttga 
      tcgttttac ggggcccgc gtttgattg aaagcttga 
      ctccgaaaa ttgtgacga cggtcggcc gttacttcg 
      acttacgat gtctcgccg agactgagg aaatgttag 
      acattgcgt acgtttgga gccataatg gcattaaaa 
      tccgcgaca tcgacatca aaaacatga attatcgtt 
      tgcgaatcg atggtgggt ttctttccg tgacaaatg 
      ccacgttta ccgacgctg gggaatacg agtgtacta 
      taaaatcaa ccgttggta aaatctcat ctcggtcgg 
      tggtcgtgg tcgaggggc ctcctggtc cgccaggtg 
      gcgtcaccg tgtccattc agaagagct cgttcacgc 
      tgcaatgga ccgatggtg ccactcacg gctcccaaa 
      tccggggtt acaccgtca gtggtcgca cgaactgga 
      accagacct gggtcaaca ttagttaca ttgttgatg 
      tgcgggaga ttgacagat acaccggcc agaaggaga 
      gtctgattg acactgcct tgactcctt ggtccactt 
      acgagtttc gcatagccg cttggaact cctggggga 
      tggggcccc cgtcagccc ccagccccc ggcattcga 
      cgccgccgg accgtccct acatcgccc catataaca 
      ttggcggtg gaggcggca agattggcg atttgacca 
      tcacgtggt cgccactca agccagacg agcagaacg 
      gtcctggca tttactaca ggatattct ggaagcgga 
      aggaccaag aagtcgaat tccagactc aggaattga 
      aggagttcg ggaacacgg gaatggcgg tagtgcaca 
      taccgccac agtactact acacggaat acattgtta 
      aagtgcaag ctattaatc agataggtc ctgggccga 
      tcagtccag aacatgtta tatactctg ccgaggaca 
      tgccgcaag ttgcgccac aactggtcg ttgctagat 
      cgttcaact cgacggcgc ttaatgtca gttgggtac 
      caattgacc agacgaggg agaaagtca gaggcaaac 
      tcatcggac atctggtgt ggcggtacg tccaaccat 
      cgttggagg aagagccgc tcaaaggct acaagattc 
      gtgtatggg aattcgatc aagacataa gcaccgcga 
      atgacacta taaaaccat tcggtggca aattagaag 
      cctacgtta ataacctac agcctggca aaacctaca 
      gaatgcgtg tgctcgcat actcgaacg gcggcgacg 
      gccgcatga gctcccccg aagtcaagt tccgaatgg 
      gtgggttta gcaatagtc cacttgcat attaccgaa 
      gtgcttgtg aggatttaa aaccattta caataacaa 
      tcgtcatcg catgtctgc ttcattatc tactaacaa 
      catgagtcc agtttttgt tatgtttta ttttgttta 
      cttcgtcaa aaggccatc atttttata tcattccga 
      cgtttgagg cttctgaac ttgtgcctt aacaggatt 
      gcattatgt aaaacgtac ctgtatcaa atgtaatac 
      tttagcaat attttttct aatgacgtg ttcaaattt 
      tctgtgctt ttacatcat aaatcataa atccgtcca 
      acacaaggt tgtagtttg tttttatct ttattatat 
      aatagaaat atacagagt atcggtgta attttttaa 
      ataaatttt ttgtaaaac a
    • >TC031494 RB
      aagatgact gtttagagt gagtgattc aattctaaa 
      acggttctc ctggttttt gttatcttt attatctct 
      tttctatga ttcggattt tatgagttg gtattatgt 
      tcttgctgt gtcttctcg ggtgccttg cactcccgg 
      tttatgcac aaggacccc aatatgaca atgctgggc 
      gaccgtaca atgcacaaa actcagttt acgaccaaa 
      atgtacctt acgcccaac aggggccac aatatcagc 
      agccccctc aagggccct tgtacaacc aggaccccc 
      agtacaatc cgcagccaa atattccaa gaatttaca 
      gtaatgacg gcctcaata acgatattt tctacgatt 
      tgaagtgcc cagaacact gggttaggt tgtatcagt 
      cgtgctata ggtttatta ggtcgccgt taaggccct 
      atgaggagg caagacgga tatgtgaag cctactctc 
      cggaagatg gcaggtcag atctagtct caattggta 
      gtacggaag aacatggct tcttgatca atcagttga 
      acgctttgg accctcaac acaggaggt ggtatatag 
      ggacgtacc aacactcag cgaactatt ggaccaatg 
      ttgacggtt cccaattag tgaatatgg aaaatgcgt 
      ttttgccgg tcgcggaac cctacggaa aagaccacc 
      tcgcgtata acttttctg tcgagctga tgcattggg 
      gcttccagc ccnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnncagga cttatacct atggtgtcg aaatcacca 
      accccgaac agatcccga ggggcccct atttcatca 
      aacaaccag tcgacgcca cgttcgata gctcgaaaa 
      aaatgctat ataacgata tcagtctga gttgtttag 
      ccggtggtt tccccaccc cgacatacg agtggttcc 
      gcgaagact acgaaatcg acaaactcg tcgcccgtc 
      gaatagacc ccttgagtg acccccgct acacaatca 
      gtggcggga tgttgataa tccacgaac cggaggaga 
      aacgtgacc atgcgacat accactgca aggcttcta 
      acaaattcg gaactatca tttccgaaa gcgtacaac 
      tcaatttcg gttttatca aagaattcg tcctgaaac 
      gctcccccg agtcgggcg acaaaaact gggggaagg 
      ccatatact gcgatcccc cgaaccact tcccttcgg 
      ttcgctact cgtggtcac gtgactact tcccgaact 
      ttgtcgagg aagaccggc gcgttttcg tatcgtatg 
      acggcgcct tgtactttt cggcgctag aaactatag 
      ataaagcca actacagtt gtagtgtcc agtcggagt 
      tttccgatt cgggccgga acggcccct ttttcccac 
      tgatggtca accctcatt cccattacc aacaactca 
      agtttccga ataatttcc ccaaagctt tccccgacg 
      cgcccattg cggggaagg aagtacgtc tagagtgta 
      tggcgtttg gatatcctg tccctagct acaactgga 
      caagacgcg gggcacctt tgccacgat ctgccgtct 
      tgtctagtt tcaacaggg tgttgacaa tccctcatg 
      tgcaggtgg aggatcagg gagagtatg tttgccggg 
      cttataacg acagagcta gcaccgaaa atagcctta 
      ttttaaata tacaagccg aacctaact tcactattc 
      cattggctg acagacacg ccgatagta aaagcgagt 
      tagtttgga cttgtgagg cgttcggaa tccctgatg 
      ttaactaca cgtggtgga aaaacggac ggcagctag 
      tcatgggtt atatggaac ccgaagaac gcgaccgaa 
      tccgaatcc aagacaacg tattgacga taaggtacc 
      tggaccaag aacgcgatc ctggaatgt atcagtgcc 
      gagcgacaa actcgctca agacaaaat actcatccg 
      cgcagctgc gcgttttag ctttcagac ccactttta 
      agaaacacc ccctcgaag cggaaactt acgccgctg 
      aagggggca gtgtgacta taaaatgta acccagagg 
      cggccccaa ggcccgagt tcgtgtgga agaaagacg 
      ggaatgttt taggttcgg gggggcatc gaagaattt 
      tagagaacg gtaatttgg ttatcagtt cggtgtccc 
      gcgatgacg aggggcagt acacgtgca gggcatcta 
      atcagttcg gagtggacg aatcgaagg gacgcttga 
      tcgttttac ggggcccgc gtttgattg aaagcttga 
      ctccgaaaa ttgtgacga cggtcggcc gttacttcg 
      acttacgat gtctcgccg agactgagg aaatgttag 
      acattgcgt acgtttgga gccataatg gcattaaaa 
      tccgcgaca tcgacatca aaaacatga attatcgtt 
      tgcgaatcg atggtgggt ttctttccg tgacaaatg 
      ccacgttta ccgacgctg gggaatacg agtgtacta 
      taaaatcaa ccgttggta aaatctcat ctcggtcgg 
      tggtcgtgg tcgaggggc ctcctggtc cgccaggtg 
      gcgtcaccg tgtccattc agaagagct cgttcacgc 
      tgcaatgga ccgatggtg ccactcacg gctcccaaa 
      tccggggtt acaccgtca gtggtcgca cgaactgga 
      accagacct gggtcaaca ttagttaca ttgttgatg 
      tgcgggaga ttgacagat acaccggcc agaaggaga 
      gtctgattg acactgcct tgactcctt ggtccactt 
      acgagtttc gcatagccg cttggaact cctggggga 
      tggggcccc cgtcagccc ccagccccc ggcattcga 
      cgccgccgg accgtccct acatcgccc catataaca 
      ttggcggtg gaggcggca agattggcg atttgacca 
      tcacgtggt cgccactca agccagacg agcagaacg 
      gtcctggca tttactaca ggatattct ggaagcgga 
      aggaccaag aagtcgaat tccagactc aggaattga 
      aggagttcg ggaacacgg gaatggcgg tagtgcaca 
      taccgccac agtactact acacggaat acattgtta 
      aagtgcaag ctattaatc agataggtc ctgggccga 
      tcagtccag aacatgtta tatactctg ccgaggaca 
      tgccgcaag ttgcgccac aactggtcg ttgctagat 
      cgttcaact cgacggcgc ttaatgtca gttgggtac 
      caattgacc agacgaggg agaaagtca gaggcaaac 
      tcatcggac atctggtgt ggcggtacg tccaaccat 
      cgttggagg aagagccgc tcaaaggct acaagattc 
      gtgtatggg aattcgatc aagacataa gcaccgcga 
      atgacacta taaaaccat tcggtggca aattagaag 
      cctacgtta ataacctac agcctggca aaacctaca 
      gaatgcgtg tgctcgcat actcgaacg gcggcgacg 
      gccgcatga gctcccccg aagtcaagt tccgaatgg 
      gtccacttg catattacc gaagtgctt gtgaggatt 
      taaaaccat ttacaataa caatcgtca tcgcatgtc 
      tgcttcatt atctactaa caacatgag tccagtttt 
      tgttatgtt ttattttgt ttacttcgt caaaaggcc 
      atcattttt atatcattc cgacgtttg aggcttctg 
      aacttgtgc cttaacagg attgcatta tgtaaaacg 
      tacctgtat caaatgtaa tactttagc aatattttt 
      tctaatgac gtgttcaaa ttttctgtg cttttacat 
      cataaatca taaatccgt ccaacacaa ggttgtagt 
      ttgttttta tctttatta tataataga aatatacag 
      agtatcggt gtaattttt taaataaat tttttgtaa 
      aaca
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