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Gene information
General information
TC013628 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2923375..2927808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013628
    iBeetle screen iB_05467

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013628 RA
      MNLEREIKL CVNGTEHKV DISCLSLDT TLNAFLRSK 
      LNLTGTKRM CLEGGCGAC IVAVQRKNH VAKKIITQA 
      INSCLVPLF SCHGWKIVT IEGLAGSQN DHHYLQKVL 
      AEFNGSQCG FCSPGMVMN MFGLLQEKK LTKQEVENS 
      FGGNICRCT GYRPILSAF KSVCDIEDI KPCPKVASR 
      KSAPCYFNL GKTTWIKVF LFDDLLQVL RTFESSTYK 
      LIAGNTSTG VYKCDGGYQ VYVDVADVD ELTSCKMEK 
      GHLVVGANI TLTETMNLF DKISQENGD FSYLKKLEK 
      HVDLIANVP VRNLGTLAG NLMIKHRHN EFPSDIFLI 
      FETVNAVLL LVDTDKNES KICVKDLLK TPMGGKLIK 
      KIILPPLSP KFKYESYKI MPRAQNAHA LVNAGFLLE 
      LNAQNIVQS ARIVFGSIN PTFVRATNT EKFLTGKKL 
      FHDEILQSA FEILDKELV PDAVPGEPE PRFRKQLAI 
      ALFYKYVLS ITPKNLISR QNQTGGVLL ERGLSSGSH 
      VYESDKSKY PLTRPMAKR EALAQASGQ AEYVMDMPD 
      RPKQLFGAF VLAKVRALS TVRKVDTSQ AMKLDGVVA 
      FFSSDDIPG RNNFTPKET NSLFFSVEE EIFCSGLVQ 
      YYNQPVGLV VATSQELAE NAASLVRVT YNAGKAPLL 
      TIQDVVKAK KESLDTEIG PKSRGKDIT HVLKGRSEL 
      SCQYHYHME TQCCSVVPT EDGLDMYPS SQWLDLSQT 
      SAATTLNIP INKINVAIR RLGGAFGGK ISRNALISS 
      AAALAAYKL KRPVKIWLP FETNMDMVG KRYPMLWDY 
      EMGVDGSGT IQYLDLTLY SDYGVGGNE PNLPYVLDA 
      VLGAYRTDF WHVKAYKVS TNNPASCYI RAPGTCEGL 
      AIIESIMEH AAVTLGIDP TDFRLKNMK AEHDLLAQF 
      VKELYKWAD IDVRKQQIK RFNEENRWR KKGLAVVPM 
      VYHFHLFGN YEVVVSVYK SDGSVAIAH GGVEMGQGI 
      NTKVIQVCA YKLKIPVEK ISVKPSNNL IAPNAHMVG 
      GSLTSETVC HGVIKACDI LLERMEPVK KQLENASWE 
      EIVQECYNQ YVNLSASSM GSPSELKNY AIYGVCSSE 
      IELDVLTGQ YIVQRVDLL EDAGTSMNA GIDMGQVEG 
      AFVMGMGYF TSEKIIFSE SGELLTNRT WNYKPPGAR 
      DVPVDFRIK FPGDTPNGV GVLNSKAIG EPPLCLACS 
      VPLAIRNAV ASARKETRN SCDEWYPFS GPSTVEDVF 
      TNCLHDFKQ YTL
    CDS sequence
    • >TC013628 RA
      atgaatcta gagcgcgaa ataaaactg tgcgtaaat 
      ggcaccgaa cacaaagtg gacatatct tgcctaagt 
      ttggacaca actttaaat gccttcctc cgatcaaaa 
      ttaaaccta actgggacc aaacgcatg tgtttggaa 
      ggcggttgt ggggcttgc attgtagca gttcagcgt 
      aaaaatcac gttgcaaaa aaaataatt actcaagcg 
      atcaattcg tgtttagtg ccacttttt tcgtgtcac 
      ggctggaaa atcgtaaca attgaggga ctagccggc 
      tctcagaat gaccatcat tacttgcaa aaagtttta 
      gcggaattt aatggcagt cagtgtgga ttctgctca 
      cctggcatg gtcatgaat atgtttggt ctcctacag 
      gagaaaaaa ttgacgaaa caggaagtt gagaattct 
      tttgggggt aatatctgt cggtgtacg gggtaccgg 
      ccgatttta agcgcgttc aagtcggtt tgtgacatt 
      gaggacata aaaccctgc cccaaagtt gcaagtcga 
      aaaagcgcc ccgtgttat tttaattta ggcaaaaca 
      acgtggatt aaggtcttt ctcttcgat gatttgttg 
      caagttttg aggactttt gagagttct acgtacaag 
      ttaattgcg ggaaacacc agcactggt gtgtataaa 
      tgtgatggt gggtatcag gtctacgtc gatgtggcc 
      gatgttgac gaacttaca tcttgtaaa atggaaaag 
      gggcacctg gtcgtgggt gctaacata actctcaca 
      gaaaccatg aatttgttt gataaaatt tcgcaagag 
      aacggtgat ttttcgtat ttaaaaaaa ttggaaaaa 
      cacgttgat ttaattgcc aatgtgcct gtcagaaac 
      ttgggaaca ttggctggt aatttaatg ataaaacat 
      cgacataat gaatttccg tccgacatt ttcctaatt 
      tttgaaacc gttaatgca gttctgttg ttagtcgat 
      actgacaaa aacgagagc aaaatatgt gtaaaagac 
      ttgctgaaa acgccaatg gggggaaaa ctgatcaaa 
      aaaatcatc ctcccgccc ttaagcccc aaatttaaa 
      tacgaaagt tacaagatc atgccgcgg gcccagaac 
      gcccacgcc ctagtcaac gcgggcttt ttgctcgaa 
      ctaaacgcc caaaacata gtgcagtct gctcgaatc 
      gtctttggt tcaatcaac cccactttt gtccgtgcc 
      acaaacacg gagaaattt ctgactggg aaaaagctt 
      ttccacgat gaaattttg caatcagct tttgaaatt 
      ttggataaa gaactcgtc cccgacgcc gttccggga 
      gaaccggag ccgcgtttc cgcaaacaa ctcgcaatc 
      gccctattt tacaagtat gttttgtcc ataacgccg 
      aaaaatttg atcagtcgc caaaatcaa acggggggt 
      gttttactg gaacggggt ttaagttcg ggtagtcat 
      gtttacgaa agcgacaag tccaagtat ccgttgact 
      cgcccaatg gcgaaaagg gaggcactt gcgcaagcc 
      tcgggccaa gccgagtat gtcatggac atgccagac 
      cggccaaag cagcttttc ggggctttt gtcttggcc 
      aaagtgcgg gcattgtca acggtacgc aaagtggac 
      acaagccag gcaatgaag ttggatggg gttgtcgcc 
      tttttctca agcgatgat attccggga cgtaacaat 
      ttcacgccc aaggagaca aactcgttg tttttttca 
      gtcgaggag gagattttt tgtagcgga ctcgtccaa 
      tattacaat caaccggtg ggtctcgtg gttgccact 
      agtcaagaa ctggccgaa aacgcagca agtttggtc 
      cgagtcacg tacaatgct gggaaagcg ccacttttg 
      actattcaa gacgtggtc aaagcaaaa aaggagagt 
      ttggacacg gaaattggg ccgaaaagt cgcggcaag 
      gacattaca catgtccta aaaggacgg tccgagttg 
      tcctgccag taccactac cacatggag acgcaatgt 
      tgcagtgtt gtgccaacg gaggatggt cttgacatg 
      tacccctcg agccaatgg ctcgactta tcgcaaact 
      tccgcagct accacactc aacatcccg ataaataag 
      atcaacgtt gcgattcgc cgattgggg ggcgcgttc 
      ggtggcaaa atctcgcga aacgcttta atctcatca 
      gcggctgct ctcgcagct tacaaattg aagcgaccg 
      gtcaaaatt tggctaccg ttcgagacc aacatggac 
      atggtcggt aaaaggtac cccatgtta tgggattac 
      gaaatgggg gttgacggt agtggcact atccagtat 
      ttggacctg actttgtac tccgactac ggtgttggg 
      ggcaacgag cccaacctt ccgtacgtc ttggacgca 
      gttttggga gcttacaga acggatttt tggcacgtt 
      aaggcctac aaagtcagt actaacaac cctgcaagt 
      tgctacatc cgagcccct ggcacgtgc gaagggttg 
      gcaatcatc gaatcgatc atggaacac gctgccgtg 
      actctcggc atagacccg accgacttc agacttaaa 
      aacatgaag gcggagcac gatttgctt gcacagttc 
      gtcaaagaa ctgtacaag tgggctgat attgacgtg 
      cgcaagcaa caaatcaag agattcaat gaggagaac 
      cgctggagg aagaaggga ttggcggtt gtgccaatg 
      gtttatcat ttccatctt tttggcaac tatgaggtc 
      gttgtttcc gtctataaa tcggacggg agtgtggca 
      atcgcgcac gggggagtc gagatgggg caaggtatc 
      aacactaag gtcattcaa gtgtgtgcc tacaagttg 
      aaaattcca gtcgaaaaa atttcggtc aagcctagc 
      aataatttg atcgcgcct aatgcccac atggtcggt 
      ggtagctta acatcggaa acagtttgt cacggtgtg 
      attaaagcg tgcgatatc ctgttagaa cgcatggaa 
      ccggtcaaa aaacagctc gaaaatgcc agttgggag 
      gaaattgtg caagaatgt tacaatcaa tatgttaac 
      ttgtcggcc tcttcaatg ggtagccct tccgaactg 
      aaaaattac gcgatttac ggcgtttgc tcctccgaa 
      atagagctg gacgtttta accgggcaa tatatcgtg 
      caaagggtt gatttactc gaagatgcc ggtactagc 
      atgaacgct ggtatcgat atggggcaa gtcgagggc 
      gcgtttgtc atgggcatg ggttatttc acgtcggag 
      aagattatt tttagcgaa agcggcgag ttgttgacc 
      aatcgcact tggaattat aaaccacca ggcgcgagg 
      gacgttcca gttgatttt aggatcaaa tttccgggc 
      gatacgccc aatggcgtt ggagttctc aattccaaa 
      gctatcggg gaaccaccg ctatgtttg gcgtgttca 
      gtcccgttg gcgatacgc aacgccgtt gcgagtgcc 
      agaaaggaa acgagaaat tcgtgcgat gaatggtat 
      cctttttcg ggaccctca actgtggaa gacgttttc 
      acgaactgc ttacacgat tttaagcaa tatacgttg 
      taa
    mRNA sequence
    • >TC013628 RA
      cagtgtcga cacaaaatg aatctagag cgcgaaata 
      aaactgtgc gtaaatggc accgaacac aaagtggac 
      atatcttgc ctaagtttg gacacaact ttaaatgcc 
      ttcctccga tcaaaatta aacctaact gggaccaaa 
      cgcatgtgt ttggaaggc ggttgtggg gcttgcatt 
      gtagcagtt cagcgtaaa aatcacgtt gcaaaaaaa 
      ataattact caagcgatc aattcgtgt ttagtgcca 
      cttttttcg tgtcacggc tggaaaatc gtaacaatt 
      gagggacta gccggctct cagaatgac catcattac 
      ttgcaaaaa gttttagcg gaatttaat ggcagtcag 
      tgtggattc tgctcacct ggcatggtc atgaatatg 
      tttggtctc ctacaggag aaaaaattg acgaaacag 
      gaagttgag aattctttt gggggtaat atctgtcgg 
      tgtacgggg taccggccg attttaagc gcgttcaag 
      tcggtttgt gacattgag gacataaaa ccctgcccc 
      aaagttgca agtcgaaaa agcgccccg tgttatttt 
      aatttaggc aaaacaacg tggattaag gtctttctc 
      ttcgatgat ttgttgcaa gttttgagg acttttgag 
      agttctacg tacaagtta attgcggga aacaccagc 
      actggtgtg tataaatgt gatggtggg tatcaggtc 
      tacgtcgat gtggccgat gttgacgaa cttacatct 
      tgtaaaatg gaaaagggg cacctggtc gtgggtgct 
      aacataact ctcacagaa accatgaat ttgtttgat 
      aaaatttcg caagagaac ggtgatttt tcgtattta 
      aaaaaattg gaaaaacac gttgattta attgccaat 
      gtgcctgtc agaaacttg ggaacattg gctggtaat 
      ttaatgata aaacatcga cataatgaa tttccgtcc 
      gacattttc ctaattttt gaaaccgtt aatgcagtt 
      ctgttgtta gtcgatact gacaaaaac gagagcaaa 
      atatgtgta aaagacttg ctgaaaacg ccaatgggg 
      ggaaaactg atcaaaaaa atcatcctc ccgccctta 
      agccccaaa tttaaatac gaaagttac aagatcatg 
      ccgcgggcc cagaacgcc cacgcccta gtcaacgcg 
      ggctttttg ctcgaacta aacgcccaa aacatagtg 
      cagtctgct cgaatcgtc tttggttca atcaacccc 
      acttttgtc cgtgccaca aacacggag aaatttctg 
      actgggaaa aagcttttc cacgatgaa attttgcaa 
      tcagctttt gaaattttg gataaagaa ctcgtcccc 
      gacgccgtt ccgggagaa ccggagccg cgtttccgc 
      aaacaactc gcaatcgcc ctattttac aagtatgtt 
      ttgtccata acgccgaaa aatttgatc agtcgccaa 
      aatcaaacg gggggtgtt ttactggaa cggggttta 
      agttcgggt agtcatgtt tacgaaagc gacaagtcc 
      aagtatccg ttgactcgc ccaatggcg aaaagggag 
      gcacttgcg caagcctcg ggccaagcc gagtatgtc 
      atggacatg ccagaccgg ccaaagcag cttttcggg 
      gcttttgtc ttggccaaa gtgcgggca ttgtcaacg 
      gtacgcaaa gtggacaca agccaggca atgaagttg 
      gatggggtt gtcgccttt ttctcaagc gatgatatt 
      ccgggacgt aacaatttc acgcccaag gagacaaac 
      tcgttgttt ttttcagtc gaggaggag attttttgt 
      agcggactc gtccaatat tacaatcaa ccggtgggt 
      ctcgtggtt gccactagt caagaactg gccgaaaac 
      gcagcaagt ttggtccga gtcacgtac aatgctggg 
      aaagcgcca cttttgact attcaagac gtggtcaaa 
      gcaaaaaag gagagtttg gacacggaa attgggccg 
      aaaagtcgc ggcaaggac attacacat gtcctaaaa 
      ggacggtcc gagttgtcc tgccagtac cactaccac 
      atggagacg caatgttgc agtgttgtg ccaacggag 
      gatggtctt gacatgtac ccctcgagc caatggctc 
      gacttatcg caaacttcc gcagctacc acactcaac 
      atcccgata aataagatc aacgttgcg attcgccga 
      ttggggggc gcgttcggt ggcaaaatc tcgcgaaac 
      gctttaatc tcatcagcg gctgctctc gcagcttac 
      aaattgaag cgaccggtc aaaatttgg ctaccgttc 
      gagaccaac atggacatg gtcggtaaa aggtacccc 
      atgttatgg gattacgaa atgggggtt gacggtagt 
      ggcactatc cagtatttg gacctgact ttgtactcc 
      gactacggt gttgggggc aacgagccc aaccttccg 
      tacgtcttg gacgcagtt ttgggagct tacagaacg 
      gatttttgg cacgttaag gcctacaaa gtcagtact 
      aacaaccct gcaagttgc tacatccga gcccctggc 
      acgtgcgaa gggttggca atcatcgaa tcgatcatg 
      gaacacgct gccgtgact ctcggcata gacccgacc 
      gacttcaga cttaaaaac atgaaggcg gagcacgat 
      ttgcttgca cagttcgtc aaagaactg tacaagtgg 
      gctgatatt gacgtgcgc aagcaacaa atcaagaga 
      ttcaatgag gagaaccgc tggaggaag aagggattg 
      gcggttgtg ccaatggtt tatcatttc catcttttt 
      ggcaactat gaggtcgtt gtttccgtc tataaatcg 
      gacgggagt gtggcaatc gcgcacggg ggagtcgag 
      atggggcaa ggtatcaac actaaggtc attcaagtg 
      tgtgcctac aagttgaaa attccagtc gaaaaaatt 
      tcggtcaag cctagcaat aatttgatc gcgcctaat 
      gcccacatg gtcggtggt agcttaaca tcggaaaca 
      gtttgtcac ggtgtgatt aaagcgtgc gatatcctg 
      ttagaacgc atggaaccg gtcaaaaaa cagctcgaa 
      aatgccagt tgggaggaa attgtgcaa gaatgttac 
      aatcaatat gttaacttg tcggcctct tcaatgggt 
      agcccttcc gaactgaaa aattacgcg atttacggc 
      gtttgctcc tccgaaata gagctggac gttttaacc 
      gggcaatat atcgtgcaa agggttgat ttactcgaa 
      gatgccggt actagcatg aacgctggt atcgatatg 
      gggcaagtc gagggcgcg tttgtcatg ggcatgggt 
      tatttcacg tcggagaag attattttt agcgaaagc 
      ggcgagttg ttgaccaat cgcacttgg aattataaa 
      ccaccaggc gcgagggac gttccagtt gattttagg 
      atcaaattt ccgggcgat acgcccaat ggcgttgga 
      gttctcaat tccaaagct atcggggaa ccaccgcta 
      tgtttggcg tgttcagtc ccgttggcg atacgcaac 
      gccgttgcg agtgccaga aaggaaacg agaaattcg 
      tgcgatgaa tggtatcct ttttcggga ccctcaact 
      gtggaagac gttttcacg aactgctta cacgatttt 
      aagcaatat acgttgtaa aataaaaaa ttgtattaa 
      ttggtttgg tttaataaa tgatagctg cgcag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY