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Gene information
General information
TC013623 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2954133..2957605
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013623
    iBeetle screen iB_05466

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013623 RA
      MTRFCSFCL FTFWTCFAA REPPTVDIP GQGRIMGKE 
      ISRYRSQKI IGYYGIPYA QPPIDDLRF APPDTSNLV 
      SWEGVRNLT DYMPACLQT ESDIREESK PFLQLIYPS 
      YSNLTTDED CLYLNVFVP FGKPPASGF ATIMWFHPG 
      NYTTGSPSM WNPHTLVYR YRVILVTFA WRLGIMGFF 
      TTMDGEAPG NFGLMDQQA AMMWVKKNI KLFGGNEDN 
      ISLMGYGTG GVSVGIHMV NTQSREYFH KAIVMSANF 
      LNPSVIKYP QEDKSLLDH LAKFSGCIR KPTSRLIEC 
      LRQLDGKPL VEQTSNINW RPLLDAGLS NTTTFLPEL 
      PRNYFERGE FHKVPFLTG YTNNEDILA VTTIIDEFE 
      TGNITNENL QELLSEIIS SDIPVNNNT DSCANNNDH 
      LTDSVMFFY GPDATNSVK DVDSLRKIV GDFTTERSH 
      GASTFLHAT YVSREQPTF VYRCDLKPV TEKAVSSIP 
      AWVSVPHLY DLIYVWGVP YWQEFDGNI KWELRDKRV 
      SEIIMTFWT SFAKSSDPT NGSLYAIRW LPFTQDNPQ 
      LMILDGSFN MSDNKVLNY KAFEFWNNY YPKVLQIAT 
      QCCNATDGS SHIQASYNV PIFSQVFLL LLHGLNCAF 
      LM
    CDS sequence
    • >TC013623 RA
      atgactcgg ttctgttca ttttgcttg tttacgttt 
      tggacgtgt tttgctgcg agggaacca cccaccgtt 
      gatatcccc gggcagggg cgcatcatg gggaaggaa 
      atctccaga tacagatct cagaaaata atcggatat 
      tacggcatc ccctacgca caaccccca atagacgac 
      ctcaggttt gcgcccccg gacacctcc aacctggtt 
      tcctgggaa ggagtgcgc aatttgact gattacatg 
      cctgcatgt ctccaaaca gagagtgac attcgagag 
      gagtctaag cccttcctt cagctcatc tacccttcg 
      tacagcaat ctcacgaca gatgaggac tgtctatac 
      ctcaacgtt ttcgttcct ttcggcaag ccccctgcc 
      agcggcttc gccaccata atgtggttc cacccgggc 
      aactacacg acgggttcg ccctccatg tggaacccc 
      cacaccttg gtgtaccgc taccgcgtg atcctggtc 
      acgttcgct tggcggttg ggcatcatg gggttcttc 
      accaccatg gacggggaa gcccctggc aacttcggc 
      ctgatggac cagcaagcg gcgatgatg tgggtcaag 
      aaaaacatc aagcttttt ggcggaaac gaggacaac 
      atcagtttg atgggttac gggaccggg ggcgtcagt 
      gtcgggata catatggtc aacacccag tcgcgcgaa 
      tatttccac aaagcgatc gtcatgagc gcgaatttt 
      ttgaaccct tctgtcatc aagtaccca caagaggac 
      aagagtttg ttggatcat ttagcgaaa ttctccggg 
      tgtatcaga aagcccacc agtcggttg atcgagtgc 
      ttgaggcag cttgatggc aagcctctg gttgagcaa 
      acctccaat atcaactgg aggccgctc ctggacgcc 
      ggtttgagc aacacgacc acgttcttg cccgagctc 
      ccgaggaat tacttcgaa aggggggag ttccataaa 
      gtccccttc ctgacgggt tataccaac aacgaggat 
      attttagcg gtgaccacg ataatcgat gagtttgaa 
      accggtaac atcacaaac gagaacctc caagagcta 
      ctttcggaa attatcagt agtgacatc cctgttaac 
      aacaacacg gactcttgt gcgaacaac aacgaccat 
      ttgaccgac tcagtcatg tttttctac ggccctgat 
      gcaaccaac agcgtcaaa gacgtggat tctttgcga 
      aaaatcgtg ggtgacttc accacggag cgaagtcac 
      ggcgcttcg actttcctg catgcgacg tacgtcagt 
      cgggagcaa ccgactttt gtctatcgg tgtgacttg 
      aaaccagtc accgaaaag gcagtcagt agtataccg 
      gcgtgggtg tcagtcccg cacttgtat gacttgatt 
      tacgtctgg ggggtgccc tattggcag gagttcgac 
      gggaacatc aagtgggaa ttgagggat aaacgcgtc 
      tccgagatc ataatgacc ttttggacg agcttcgcc 
      aaaagctca gaccccaca aatgggagt ctgtacgcc 
      atacggtgg ctgccgttc acacaggac aacccgcag 
      ctgatgatt ctcgacggc tcgtttaac atgagcgat 
      aacaaagtg ttgaattac aaagcgttc gaattttgg 
      aataattac tacccgaaa gtgctgcaa attgccaca 
      cagtgttgc aatgccacc gatgggtcg tcccacatt 
      caggcttcc tacaacgta ccaatcttt tcccaagtc 
      tttctatta cttttacac ggtttaaat tgtgccttc 
      ctcatgtaa
    mRNA sequence
    • >TC013623 RA
      cctttgctt tctcaaaga cacagtgtt aagtgcagt 
      gaagaaaaa atgactcgg ttctgttca ttttgcttg 
      tttacgttt tggacgtgt tttgctgcg agggaacca 
      cccaccgtt gatatcccc gggcagggg cgcatcatg 
      gggaaggaa atctccaga tacagatct cagaaaata 
      atcggatat tacggcatc ccctacgca caaccccca 
      atagacgac ctcaggttt gcgcccccg gacacctcc 
      aacctggtt tcctgggaa ggagtgcgc aatttgact 
      gattacatg cctgcatgt ctccaaaca gagagtgac 
      attcgagag gagtctaag cccttcctt cagctcatc 
      tacccttcg tacagcaat ctcacgaca gatgaggac 
      tgtctatac ctcaacgtt ttcgttcct ttcggcaag 
      ccccctgcc agcggcttc gccaccata atgtggttc 
      cacccgggc aactacacg acgggttcg ccctccatg 
      tggaacccc cacaccttg gtgtaccgc taccgcgtg 
      atcctggtc acgttcgct tggcggttg ggcatcatg 
      gggttcttc accaccatg gacggggaa gcccctggc 
      aacttcggc ctgatggac cagcaagcg gcgatgatg 
      tgggtcaag aaaaacatc aagcttttt ggcggaaac 
      gaggacaac atcagtttg atgggttac gggaccggg 
      ggcgtcagt gtcgggata catatggtc aacacccag 
      tcgcgcgaa tatttccac aaagcgatc gtcatgagc 
      gcgaatttt ttgaaccct tctgtcatc aagtaccca 
      caagaggac aagagtttg ttggatcat ttagcgaaa 
      ttctccggg tgtatcaga aagcccacc agtcggttg 
      atcgagtgc ttgaggcag cttgatggc aagcctctg 
      gttgagcaa acctccaat atcaactgg aggccgctc 
      ctggacgcc ggtttgagc aacacgacc acgttcttg 
      cccgagctc ccgaggaat tacttcgaa aggggggag 
      ttccataaa gtccccttc ctgacgggt tataccaac 
      aacgaggat attttagcg gtgaccacg ataatcgat 
      gagtttgaa accggtaac atcacaaac gagaacctc 
      caagagcta ctttcggaa attatcagt agtgacatc 
      cctgttaac aacaacacg gactcttgt gcgaacaac 
      aacgaccat ttgaccgac tcagtcatg tttttctac 
      ggccctgat gcaaccaac agcgtcaaa gacgtggat 
      tctttgcga aaaatcgtg ggtgacttc accacggag 
      cgaagtcac ggcgcttcg actttcctg catgcgacg 
      tacgtcagt cgggagcaa ccgactttt gtctatcgg 
      tgtgacttg aaaccagtc accgaaaag gcagtcagt 
      agtataccg gcgtgggtg tcagtcccg cacttgtat 
      gacttgatt tacgtctgg ggggtgccc tattggcag 
      gagttcgac gggaacatc aagtgggaa ttgagggat 
      aaacgcgtc tccgagatc ataatgacc ttttggacg 
      agcttcgcc aaaagctca gaccccaca aatgggagt 
      ctgtacgcc atacggtgg ctgccgttc acacaggac 
      aacccgcag ctgatgatt ctcgacggc tcgtttaac 
      atgagcgat aacaaagtg ttgaattac aaagcgttc 
      gaattttgg aataattac tacccgaaa gtgctgcaa 
      attgccaca cagtgttgc aatgccacc gatgggtcg 
      tcccacatt caggcttcc tacaacgta ccaatcttt 
      tcccaagtc tttctatta cttttacac ggtttaaat 
      tgtgccttc ctcatgtaa aaatttcaa atgttcgga 
      gaatttcta ggatgtaga taataataa ttgtcacaa 
      tgtaaatag tcgtttgat gattcctta gactgtggt 
      agctacgac acctcaact ttatcacaa cataaacaa 
      ttttgtttg agatgaagt agctattta taagtggtt 
      gaatttcat gttctcacg catttaact acttataac 
      aatggtaat tgataacac aaaggtcgt atttttctg 
      atgtaaaaa tttgctaaa tatactatg ataattaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY