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Gene information
General information
TC013622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2970299..2974976
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013622
    iBeetle screen iB_05465

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 3

    egg chamber mostly not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013622 RA
      MSHNNSDQT PLSQPLKVE TDNNSVHAE TANYYEEDK 
      DNFNQGNDN EDSDSLGPL PPKWEKAYT ESGEVYFID 
      HSTGTSHWL DPRLSKFQK KSLEDCLDD ELPYGWEKI 
      SDPNYGTYF IDHVNRRTQ YENPVIQAK RAASQASAR 
      ASRTSFTKD PAELCGQRF RTSLVKSSR GLGFTIVGG 
      DDGVDEFLQ IKSVVPKGP AWLDGKLQT GDVIVYVND 
      TCVLGYTHI QIVRLFQSI NVGSTVSLE VCRGYPLPF 
      DPNDPNTEV VTTIAVDSH LQQVAADKR LLDTNYNFL 
      DGDDMSREE DNTLEPSDD IDDLIKGIG NCTLGKVEV 
      RVRIVKGNL GFGFTIADS ACGQRVKQI LDRQRCKNL 
      MEGDILLEI NDISLINMS HDEVVQVLK NCPYNSEAT 
      ICVQRGCTN KTPNIRNKL RKLDNRFSG KDINNAYRS 
      KTPTADIYS TQPREVLPS RPKTPLVDT RSLSKTPVK 
      DLNSNSNNE LYDNFTADR SRLRLSLID NPEEEDDLD 
      ISENGPTKP SYEYPPPVV DPKYTWGML MMHQYDCTC 
      YNCTNPRPA VENCDSFDH SNLKKNVGM DYWQYMPRN 
      AEYVTTTVV LHRQETGFG FRIVGGIED KSQVAVGHI 
      VAGGAADMD GRIRSGDEI VSVDGYSVL KASHRQVVR 
      LMNAAAARG QVTLVVRRR VYPPQPVMA PYPMNWPHE 
      NPPPPPASH LSVMPPPHA EGNYDVTVQ RNENEGFGF 
      VIISSANKQ GSTIGRLIE DSPAEKCGR LRVGDHIVA 
      VNHIAIMHM SHGDIVNLI KESGLSVTL TITPSFDL
    CDS sequence
    • >TC013622 RA
      atgagtcac aataacagt gaccaaaca cctctttcc 
      cagccactg aaggtagag acggacaat aacagtgtg 
      cacgccgaa actgcgaat tactacgag gaggataag 
      gacaatttc aatcagggc aatgataac gaagacagc 
      gactcgctg ggacccctg cccccgaag tgggagaag 
      gcctacact gaaagtgga gaagtttac ttcattgac 
      cactccacg ggcacgtcg cattggctg gacccccgc 
      ttgtcgaag ttccagaag aagtcgctc gaagactgt 
      ctggacgac gaactgccc tacggctgg gagaagatc 
      agcgacccg aactacggc acctacttc atagaccac 
      gtaaatcgg cgaactcag tacgaaaac cctgtgata 
      caagcgaaa agagccgca tctcaagcc tccgctcgt 
      gcgagtcgg acgtcgttc acgaaggat ccggcggag 
      ctgtgcggg cagcggttc cgcacgtcc cttgtcaag 
      tcttctaga ggtttaggc ttcacgatt gtcggcggc 
      gatgacggc gttgacgag tttttacag attaaatct 
      gtggttcct aaaggaccg gcttggctg gatgggaaa 
      cttcaaacg ggagacgtc attgtctac gttaatgat 
      acttgcgtt ttggggtac actcacatt cagattgtt 
      cggttgttt caatcgatt aatgtcggt tcgactgtc 
      tcgcttgaa gtttgccga gggtaccct cttccgttc 
      gaccctaat gaccccaat actgaagtt gtgactacg 
      atcgctgtg gactcgcat ttacagcaa gtcgcggct 
      gataagagg cttctagac acgaattat aatttctta 
      gacggggac gatatgtcg cgggaggaa gacaacaca 
      cttgagccg tcagacgac atcgatgat ttaatcaaa 
      ggaatcgga aactgtact ttggggaaa gttgaagtt 
      agggtgcgc atagttaag ggcaatctc ggatttggg 
      ttcacaata gccgacagt gcttgtggt caaagagtc 
      aagcagata cttgacaga cagcggtgc aagaacctg 
      atggaaggc gatatttta ttagagata aacgacatt 
      tctttgatc aacatgtcg cacgacgaa gtcgtgcaa 
      gtcctgaag aactgtcct tacaacagc gaagctaca 
      atttgtgtt cagagagga tgcacaaac aaaacacca 
      aatattaga aataagtta cgtaaatta gacaatagg 
      ttcagcggt aaagatatc aacaatgcc taccgcagc 
      aaaacacca actgctgat atctacagc actcaaccg 
      cgggaagtt ttaccaagt cgtccaaag acaccgctc 
      gttgacacg cggagcctt tcgaaaaca ccagtcaaa 
      gacctcaac tcgaactca aacaatgaa ctctacgac 
      aacttcact gccgaccgt tccagacta agactatca 
      ctcatcgat aaccccgag gaagaagac gacttagac 
      atatcagaa aacgggcca accaaaccc tcctacgaa 
      taccccccg cctgtggtc gaccccaaa tacacctgg 
      ggcatgctg atgatgcac cagtacgac tgcacgtgc 
      tacaactgt acaaacccg cgcccagcg gtcgaaaac 
      tgcgacagc ttcgaccac agcaacctg aagaaaaac 
      gtcgggatg gactactgg cagtacatg ccgcgaaac 
      gccgaatac gtcaccaca acggtggtg ttgcaccgg 
      caggaaacc ggcttcggc ttccggatc gttggcgga 
      atcgaggac aagtcccaa gtggcggtt ggacacata 
      gtcgcagga ggggccgca gacatggac ggccgcatt 
      cggtcaggg gacgaaatc gtcagtgtt gacggttac 
      tcagtgttg aaagcttcg caccgccaa gtcgttcgg 
      ctgatgaac gcggcggcg gcgcgaggt caggtcacc 
      ttggtcgtc cggagacgc gtctacccg ccacagccc 
      gtcatggcc ccctatccc atgaactgg ccccacgaa 
      aacccgcca ccgccccca gcttcgcac ttgtcggtc 
      atgccgccc ccacacgct gagggcaac tacgacgtc 
      acggtgcag cggaacgaa aacgaaggc ttcgggttt 
      gtcatcatc tcgtcagcc aacaagcaa ggctccacc 
      atcgggcgg ctgatcgag gacagcccg gcggagaag 
      tgcggccgc ctgcgagtt ggcgaccac atagttgca 
      gtcaaccac attgcgatt atgcacatg agtcacggt 
      gatattgtt aatctgata aaagagtcg ggcttgtcc 
      gttactctc acgattact cccagtttc gatctttga
    mRNA sequence
    • >TC013622 RA
      ttctagaaa aatcgtcga tacttgttc cgcgctaca 
      ttttcaaat accgtttct tgtgacgtg tctaaagtg 
      ccgcgcttt gaccaacgg aagaaacat gtgcgtagc 
      tttagccac cagtcgtgt taaaaaaac agtggagta 
      acgaaacga ggaaattct tcgttagcg tttgcaagt 
      tttccgaca aaagtgaag aaaatcacg cacgatatg 
      tgaagtacc agtttattt acgcgaatt ggcaataat 
      aatggcccc gactgtggg aggacgcac ttaacctgc 
      aataaatat ttatgttgg gctataaat gaagtggaa 
      acaatttat ctcagtgtg ttccccatc ccaacgacg 
      aaaataatg ctgcttgag ggcgcaagg caccgatcg 
      ctgggcccc cggaaatat taaaaagca attcatgtg 
      agaactggc caagatgag tcacaataa cagtgacca 
      aacacctct ttcccagcc actgaaggt agagacgga 
      caataacag tgtgcacgc cgaaactgc gaattacta 
      cgaggagga taaggacaa tttcaatca gggcaatga 
      taacgaaga cagcgactc gctgggacc cctgccccc 
      gaagtggga gaaggccta cactgaaag tggagaagt 
      ttacttcat tgaccactc cacgggcac gtcgcattg 
      gctggaccc ccgcttgtc gaagttcca gaagaagtc 
      gctcgaaga ctgtctgga cgacgaact gccctacgg 
      ctgggagaa gatcagcga cccgaacta cggcaccta 
      cttcataga ccacgtaaa tcggcgaac tcagtacga 
      aaaccctgt gatacaagc gaaaagagc cgcatctca 
      agcctccgc tcgtgcgag tcggacgtc gttcacgaa 
      ggatccggc ggagctgtg cgggcagcg gttccgcac 
      gtcccttgt caagtcttc tagaggttt aggcttcac 
      gattgtcgg cggcgatga cggcgttga cgagttttt 
      acagattaa atctgtggt tcctaaagg accggcttg 
      gctggatgg gaaacttca aacgggaga cgtcattgt 
      ctacgttaa tgatacttg cgttttggg gtacactca 
      cattcagat tgttcggtt gtttcaatc gattaatgt 
      cggttcgac tgtctcgct tgaagtttg ccgagggta 
      ccctcttcc gttcgaccc taatgaccc caatactga 
      agttgtgac tacgatcgc tgtggactc gcatttaca 
      gcaagtcgc ggctgataa gaggcttct agacacgaa 
      ttataattt cttagacgg ggacgatat gtcgcggga 
      ggaagacaa cacacttga gccgtcaga cgacatcga 
      tgatttaat caaaggaat cggaaactg tactttggg 
      gaaagttga agttagggt gcgcatagt taagggcaa 
      tctcggatt tgggttcac aatagccga cagtgcttg 
      tggtcaaag agtcaagca gatacttga cagacagcg 
      gtgcaagaa cctgatgga aggcgatat tttattaga 
      gataaacga catttcttt gatcaacat gtcgcacga 
      cgaagtcgt gcaagtcct gaagaactg tccttacaa 
      cagcgaagc tacaatttg tgttcagag aggatgcac 
      aaacaaaac accaaatat tagaaataa gttacgtaa 
      attagacaa taggttcag cggtaaaga tatcaacaa 
      tgcctaccg cagcaaaac accaactgc tgatatcta 
      cagcactca accgcggga agttttacc aagtcgtcc 
      aaagacacc gctcgttga cacgcggag cctttcgaa 
      aacaccagt caaagacct caactcgaa ctcaaacaa 
      tgaactcta cgacaactt cactgccga ccgttccag 
      actaagact atcactcat cgataaccc cgaggaaga 
      agacgactt agacatatc agaaaacgg gccaaccaa 
      accctccta cgaataccc cccgcctgt ggtcgaccc 
      caaatacac ctggggcat gctgatgat gcaccagta 
      cgactgcac gtgctacaa ctgtacaaa cccgcgccc 
      agcggtcga aaactgcga cagcttcga ccacagcaa 
      cctgaagaa aaacgtcgg gatggacta ctggcagta 
      catgccgcg aaacgccga atacgtcac cacaacggt 
      ggtgttgca ccggcagga aaccggctt cggcttccg 
      gatcgttgg cggaatcga ggacaagtc ccaagtggc 
      ggttggaca catagtcgc aggaggggc cgcagacat 
      ggacggccg cattcggtc aggggacga aatcgtcag 
      tgttgacgg ttactcagt gttgaaagc ttcgcaccg 
      ccaagtcgt tcggctgat gaacgcggc ggcggcgcg 
      aggtcaggt caccttggt cgtccggag acgcgtcta 
      cccgccaca gcccgtcat ggcccccta tcccatgaa 
      ctggcccca cgaaaaccc gccaccgcc cccagcttc 
      gcacttgtc ggtcatgcc gcccccaca cgctgaggg 
      caactacga cgtcacggt gcagcggaa cgaaaacga 
      aggcttcgg gtttgtcat catctcgtc agccaacaa 
      gcaaggctc caccatcgg gcggctgat cgaggacag 
      cccggcgga gaagtgcgg ccgcctgcg agttggcga 
      ccacatagt tgcagtcaa ccacattgc gattatgca 
      catgagtca cggtgatat tgttaatct gataaaaga 
      gtcgggctt gtccgttac tctcacgat tactcccag 
      tttcgatct ttgatatgt taatggtaa atcgagttt 
      tttcgcgaa atcaaaaaa gtttttcag taaagtgtt 
      ctggattta aaatgttat cgaatcgaa tatttataa 
      gaaatttat cgttgcctt ataccaaat tgtattaag 
      atttgtgat attggtttg atcgcactg ccttgacga 
      aactagaaa ttcaaatgt ttcgtttgt ctgacgcga 
      ctgattttt gttttattt taatttatt tttaatcaa 
      aaccaatgt gatggttgt cggctctaa atcaattga 
      tgcattttg ctcaaaaac agtttattg ttaacgtat 
      ttttaaaat taatagctc attagaagt aatgtaggg 
      tatgtaaaa tcactgaat ctcagcatg taataatcg 
      tcaatattt ttgttacct ttaaaactt gtacttgct 
      acattattt attgtatat tttttattc tttgtttta 
      taatacttt aattttatg ttacctccc tatattata 
      cctagatta agttattat aaaatattg caatataat 
      ctaaccaaa tactataaa taggcgtgt ttggcggta 
      gaaagtctc aatttggtt attttcgaa tcttagtgg 
      gcttgtcca caggtgatc tcgtggctc cgtaaagcc 
      aactactct ataatcaaa aactctgta gctcagtag 
      gccataaac tgtattact aattttatc tcacttggt 
      agaatctta acacgcgga gtttttctc ccatcaatt 
      atggcataa aaacttcgc gtcgcacgg tataattat 
      atgattatg tgatacaca gttatttta tatttagca 
      atattatga atttacgta ttgtgcctt taattataa 
      cttgccgtt aatttatct tatgtatct tgagtctgt 
      gtaaagtcg ttccttggt ggcaaaaca ggtgttatt 
      aataaaatt cattccggc cagagcatt tgaaattta 
      ttactagac tgattatgt gtaaaaagt tgtatagta 
      ctcacaaat atattcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY