Status
Please wait ...
Gene information
General information
TC013614 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3099947..3118696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013614
    iBeetle screen iB_05464

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013614 RA
      MSSDVKEEK TDADEKTAD SGVIKAFCG GNRTLSWHE 
      HVYKKLTNK PTPHLIENI LGIQNEKPE AKRMIVSIA 
      DNQEDFPKL QPAGTGCEV NEPLNLSIR SDLKVRTKT 
      VKEPTRKRK KTREPLDLK LSPAPLHPP HPTESVAED 
      GDAKSKKKK ARTTFTGRQ IFELEKQFE MKKYLSSSE 
      RSEMAKLLN VTETQVKIW FQNRRTKWK KIDNISNAE 
      AAEHKNHTI PKDGSKSSD DVKLRTYSK HASQSSNSS 
      IESDSKSSV ASAEPPLQA KSFKDILQN SRTNPLSPD 
      PHFQAQMDS TKDVLLNLS FNIKDKS
    CDS sequence
    • >TC013614 RA
      atgtcatct gacgtcaag gaggaaaag accgacgcc 
      gacgagaag accgctgac agtggcgtg atcaaggcg 
      ttttgcggt gggaacagg acgctgtcc tggcacgag 
      cacgtctac aagaagctc accaacaag cccacgccg 
      cacctcatc gagaacatc ctcggcatc cagaacgag 
      aagccggag gccaagagg atgatcgtc tccattgcc 
      gacaaccag gaggacttc cccaagctg caacccgcc 
      ggcacgggc tgtgaagtt aatgaacct ttaaacttg 
      tcaattcgt agtgacctt aaagtgcga actaagact 
      gttaaagag ccgacgcga aagagaaaa aagactcgt 
      gaaccgttg gatttgaag ttatcaccg gcccccttg 
      cacccgccc catccgact gaatccgta gcggaagac 
      ggcgatgct aaaagtaag aagaaaaaa gctcggacc 
      acgttcaca ggaagacag attttcgag ttggaaaag 
      cagttcgaa atgaagaag tatctgagt agtagcgag 
      cgatccgaa atggcgaag ctacttaat gttacagaa 
      actcaggtg aagatctgg ttccaaaat cggcggacc 
      aagtggaag aagatcgac aacatttcg aacgcggag 
      gcggcagag cacaaaaat cacacgatc ccgaaggac 
      ggctccaag tcctccgac gacgtcaag ctgcgcacg 
      tactcgaag cacgccagc cagagctcc aactcgagc 
      atcgagagc gactccaag agctcggtg gcgtcggcg 
      gagccgccg ctccaagcg aaatctttc aaggatatt 
      ttgcaaaat tcgcgcaca aacccgctg tctcccgac 
      ccccacttc caggcccag atggactct accaaagac 
      gttctcttg aacttatcc tttaatatt aaagacaaa 
      tcctaa
    mRNA sequence
    • >TC013614 RA
      ccaacaaaa atcacctag actcttaaa aatcgaagt 
      caaatagct gtaaattta ctggcgccg gcttgcaga 
      atgtcccag attttacaa caaaaccgc atgtgtgtc 
      ttaattaaa ttaaaataa ttctgttca tttgagggg 
      caaaacgac aaacccgac ttatccaaa actgttgat 
      ataatgttt atttccaac ctgttgaca cgtgggtgc 
      tccccgctg ccttttttg gtactatga agcgatcag 
      aatatttct gtttatgga atgtgttga ttttaagta 
      tttctgctt tctgaattt ttacatggt cgtatcttt 
      ccctgcttc tgagtttgc cacagtgcg agtcgcgtt 
      gtgcgaaag ctcgcggct ggtggtggg ggtgtatgc 
      agtgtaggt atgcggcaa ttagcgaag tttaagttg 
      attaggtca tgctagtga tcattgttt gctcataaa 
      gctccagtg gctgttaga tgcgccgtt cagtggcgc 
      accccggcg atcagtagc gctgagctt tcgtagcac 
      gcggcggcg gcggcagct ttggggggc tgcgtgtcg 
      gtgagtttc agtttcagt cagacggaa gacggagtc 
      tcggtcgca ttggcgttg aagtagtgc gcgcgcggc 
      gtcgaacac gcggttgat cctgtcaac agaagagca 
      agtggcgcg tgctgggac ttgatgctg cttatcagc 
      gatgtcatc tgacgtcaa ggaggaaaa gaccgacgc 
      cgacgagaa gaccgctga cagtggcgt gatcaaggc 
      gttttgcgg tgggaacag gacgctgtc ctggcacga 
      gcacgtcta caagaagct caccaacaa gcccacgcc 
      gcacctcat cgagaacat cctcggcat ccagaacga 
      gaagccgga ggccaagag gatgatcgt ctccattgc 
      cgacaacca ggaggactt ccccaagct gcaacccgc 
      cggcacggg ctgtgaagt taatgaacc tttaaactt 
      gtcaattcg tagtgacct taaagtgcg aactaagac 
      tgttaaaga gccgacgcg aaagagaaa aaagactcg 
      tgaaccgtt ggatttgaa gttatcacc ggccccctt 
      gcacccgcc ccatccgac tgaatccgt agcggaaga 
      cggcgatgc taaaagtaa gaagaaaaa agctcggac 
      cacgttcac aggaagaca gattttcga gttggaaaa 
      gcagttcga aatgaagaa gtatctgag tagtagcga 
      gcgatccga aatggcgaa gctacttaa tgttacaga 
      aactcaggt gaagatctg gttccaaaa tcggcggac 
      caagtggaa gaagatcga caacatttc gaacgcgga 
      ggcggcaga gcacaaaaa tcacacgat cccgaagga 
      cggctccaa gtcctccga cgacgtcaa gctgcgcac 
      gtactcgaa gcacgccag ccagagctc caactcgag 
      catcgagag cgactccaa gagctcggt ggcgtcggc 
      ggagccgcc gctccaagc gaaatcttt caaggatat 
      tttgcaaaa ttcgcgcac aaacccgct gtctcccga 
      cccccactt ccaggccca gatggactc taccaaaga 
      cgttctctt gaacttatc ctttaatat taaagacaa 
      atcctaaag acggaggtc ttttagcga caaatttgt 
      aacacgaag agtacaatt ccgttagtt gcacataca 
      tatattaga agaatattt ttcataact gatttagat 
      gtattggta aacttcata ttttgtatg taaatacca 
      ctatgtttg taatgatcc atttcatgt aatatcatc 
      attgaataa acagatcgt ttcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY