| General information | |||
| TC013590 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3331599..3358402 | |
| Sequence information | |||
| Transcripts / Proteins (6) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013590 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05460 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
- >TC013590 RA
MEVKGLKSL APNRIVYCT MEVENGEKL QTDQAEASK PMWDTQGDF CTTHPLPIV KVKLYTENP GMLALEDKE LGKVILKPT PLSSKVPEW HKMTVPKNL PDQDLRIRI ACRMDKPLN MKHCGYLYA LGKNVWKKW KKRYYVLVQ VSQYTFAMC SYKEKKSEP SEMMQLDGY TVDYIEASS ANLMVGMGK HLEGGRYFF NAVKEGDSI IYASDDENE CHLWVMAMY RATGQSHKP TPPLTLQDK NSTISKIQG DADRARKHG MEDFISADP CQFDHAALF KLLQNLTLE YRLNDPYCS LGWFSPGQV FVLDEYCAR YGVRGCYRH LCYLSDLLD RAEKNIMID PTLIHYSFA FCASHVHGN RPDGVGSVT HEEKEKFQE VKERLRVLL ENQITNFRY CFPFGRPEG ALKATLSLL ERVLMKDIV TPVPPEEVR GMIKKCLET AALLNYTRL SSEAKIEED LRGETMVPP SKKLDDLIH LAELCVDLL QQNEEHYAE AFAWFSDLL VEHAEIFWS LFAVDMDRV LAEQPADTW DSFPLFQIL NDYLRTDDN LKNGRFHQH LRDTFAPLV VRYVDLMES SIAQSIHKG FEKERWEIK GNGCATSED LFWKLDALQ SFIRDLHWP DAEFRQHLE QRLKLMACD MIESCIQRT ETAFQQWLK KSVTFISTD YIIPSEMCA MVNVILDAK NQSLKLCAV DGIDVHQYH TKIDDQIDK TLANMNQGM ISKLMSVLE ATLSKLSRY DEGSLIGSI LSFTNVSGS GKDMGQGYV NFTRNCMDQ IRGKVTDEL WILNFFEQW YTSQINMLC NWLSERLDH TLHPYQCTC LAHIVKKIY SDFELQGVM EDKLNSKAY QTIAQRMQT EEATCALTM GQAEGGSEG LDEDGDNRS KTKVTIMES LSGEDGNYV SNIGNMVAG RVGNIFGKG LGGISTKFG GGTSSWF
- >TC013590 RB
MTVPKNLPD QDLRIRIAC RMDKPLNMK HCGYLYALG KNVWKKWKK RYYVLVQVS QYTFAMCSY KEKKSEPSE MMQLDGYTV DYIEASSAN LMVGMGKHL EGGRYFFNA VKEGDSIIY ASDDENECH LWVMAMYRA TGQSHKPTP PLTLQDKNS TISKIQGDA DRARKHGME DFISADPCQ FDHAALFKL LQNLTLEYR LNDPYCSLG WFSPGQVFV LDEYCARYG VRGCYRHLC YLSDLLDRA EKNIMIDPT LIHYSFAFC ASHVHGNRP DGVGSVTHE EKEKFQEVK ERLRVLLEN QITNFRYCF PFGRPEGAL KATLSLLER VLMKDIVTP VPPEEVRGM IKKCLETAA LLNYTRLSS EAKIEEDLR GETMVPPSK KLDDLIHLA ELCVDLLQQ NEEHYAEAF AWFSDLLVE HAEIFWSLF AVDMDRVLA EQPADTWDS FPLFQILND YLRTDDNLK NGRFHQHLR DTFAPLVVR YVDLMESSI AQSIHKGFE KERWEIKGN GCATSEDLF WKLDALQSF IRDLHWPDA EFRQHLEQR LKLMACDMI ESCIQRTET AFQQWLKKS VTFISTDYI IPSEMCAMV NVILDAKNQ SLKLCAVDG IDVHQYHTK IDDQIDKTL ANMNQGMIS KLMSVLEAT LSKLSRYDE GSLIGSILS FTNVSGSGK DMGQGYVNF TRNCMDQIR GKVTDELWI LNFFEQWYT SQINMLCNW LSERLDHTL HPYQCTCLA HIVKKIYSD FELQGVMED KLNSKAYQT IAQRMQTEE ATCALTMGQ AEGGSEGLD EDGDNRSKT KVTIMESLS GEDGNYVSN IGNMVAGRV GNIFGKGLG GISTKFGGG TSSWF
- >TC013590 RC
MTVPKNLPD QDLRIRIAC RMDKPLNMK HCGYLYALG KNVWKKWKK RYYVLVQVS QYTFAMCSY KEKKSEPSE MMQLDGYTV DYIEASSAN LMVGMDLEG GRYFFNAVK EGDSIIYAS DDENECHLW VMAMYRATG QSHKPTPPL TLQDKNSTI SKIQGDADR ARKHGMEDF ISADPCQFD HAALFKLLQ NLTLEYRLN DPYCSLGWF SPGQVFVLD EYCARYGVR GCYRHLCYL SDLLDRAEK NIMIDPTLI HYSFAFCAS HVHGNRPDG VGSVTHEEK EKFQEVKER LRVLLENQI TNFRYCFPF GRPEGALKA TLSLLERVL MKDIVTPVP PEEVRGMIK KCLETAALL NYTRLSSEA KIEEDLRGE TMVPPSKKL DDLIHLAEL CVDLLQQNE EHYAEAFAW FSDLLVEHA EIFWSLFAV DMDRVLAEQ PADTWDSFP LFQILNDYL RTDDNLKNG RFHQHLRDT FAPLVVRYV DLMESSIAQ SIHKGFEKE RWEIKGNGC ATSEDLFWK LDALQSFIR DLHWPDAEF RQHLEQRLK LMACDMIES CIQRTETAF QQWLKKSVT FISTDYIIP SEMCAMVNV ILDAKNQSL KLCAVDGID VHQYHTKID DQIDKTLAN MNQGMISKL MSVLEATLS KLSRYDEGS LIGSILSFT NVSGSGKDM GQGYVNFTR NCMDQIRGK VTDELWILN FFEQWYTSQ INMLCNWLS ERLDHTLHP YQCTCLAHI VKKIYSDFE LQGVMEDKL NSKAYQTIA QRMQTEEAT CALTMGQAE GGSEGLDED GDNRSKTKV TIMESLSGE DGNYVSNIG NMVAGRVGN IFGKGLGGI STKFGGGTS SWF
- >TC013590 RD
MIDPSSSEE DSEEEHAPP HAGHHPAAH HHHHQTTHH HHHPSEASP YHAHLSDSG SGNLEVPNN TAALQRSLS PSSAVSAET LSHSIGSSR ANSLPRPTS PSPSVASEK TEVDLQEKQ EREEEERKR RIQLYVFIS RCIAYPFNA KQPTDMTRR QTKITKHQL ETITARFQS FLKGETQIM ADEAFQNAV QNYYDVFLK SERVVKMVQ SGACSQYDF REVFRNNIE KRVRSLPEM EGLSKETVF TSWMAKFDC ILKGLGDED SKRPSRMQQ QSLNSELIL SKEQLYDMF QQILGVKKF EHQLLFNAL MLDSADEQA AAIRRELDG RMQKVAEME KNRKLMPKF VLKEMESLY IEELKSSIN LLMANLESL PVSKGSMDS KYGLQKLKR YNHRLHNHN NPYNVKHLL MNYVTSFRS QGSLAKLEG DSADSDTQL TKLDVVLTF QLEVVVMEV KGLKSLAPN RIVYCTMEV ENGEKLQTD QAEASKPMW DTQGDFCTT HPLPIVKVK LYTENPGML ALEDKELGK VILKPTPLS SKV
- >TC013590 RE
MIDPSSSEE DSEEEHAPP HAGHHPAAH HHHHQTTHH HHHPSEASP YHAHLSDSG SGNLEVPNN TAALQRSLS PSSAVSAET LSHSIGSSR ANSLPRPTS PSPSVASEK TEVDLQEKQ EREEEERKR RIQLYVFIS RCIAYPFNA KQPTDMTRR QTKITKHQL ETITARFQS FLKGETQIM ADEAFQNAV QNYYDVFLK SERVVKMVQ SGACSQYDF REVFRNNIE KRVRSLPEM EGLSKETVF TSWMAKFDC ILKGLGDED SKRPSRMQQ QSLNSELIL SKEQLYDMF QQILGVKKF EHQLLFNAL MLDSADEQA AAIRRELDG RMQKVAEME KNRKLMPKF VLKEMESLY IEELKSSIN LLMANLESL PVSKGSMDS KYGLQKLKR YNHRSQGSL AKLEGDSAD SDTQLTKLD VVLTFQLEV IFAHCSTSI FHSRSVLSS NLIDSPLLS RIIKTFSVL MPLQDLVHL FPILLTCYV LHIRPPSL
- >TC013590 RF
MIDPSSSEE DSEEEHAPP HAGHHPAAH HHHHQTTHH HHHPSEASP YHAHLSDSG SGNLEVPNN TAALQRSLS PSSAVSAET LSHSIGSSR ANSLPRPTS PSPSVASEK TEVDLQEKQ EREEEERKR RIQLYVFIS RCIAYPFNA KQPTDMTRR QTKITKHQL ETITARFQS FLKGETQIM ADEAFQNAV QNYYDVFLK SERVVKMVQ SGACSQYDF REVFRNNIE KRVRSLPEM EGLSKETVF TSWMAKFDC ILKGLGDED SKRPSRMQQ QSLNSELIL SKEQLYDMF QQILGVKKF EHQLLFNAL MLDSADEQA AAIRRELDG RMQKVAEME KNRKLMPKF VLKEMESLY IEELKSSIN LLMANLESL PVSKGSMDS KYGLQKLKR YNHRLHNHN NPYNVKHLL MNYVTSFRS QGSLAKLEG DSADSDTQL TKLDVVLTF QLEVIFAHC STSIFHSRS VLSSNLIDS PLLSRIIKT FSVLMPLQD LVHLFPILL TCYVLHIRP PSL
- >TC013590 RA
atggaagta aaaggttta aaatctcta gcgccaaat cgaatcgtg tattgcacc atggaggtc gagaacgga gaaaagctc caaactgac caggccgaa gcctcgaaa ccgatgtgg gacacgcag ggcgatttt tgcacgacc caccctttg ccaattgtc aaagtaaaa ctgtacacc gaaaacccc ggaatgcta gccctcgaa gacaaagaa ctcgggaaa gtcatcctc aaaccgacg cccctctcg tcaaaggta ccggaatgg cacaaaatg actgtacct aaaaacctc cccgatcaa gacttacgg attagaata gcgtgtaga atggacaag cctttgaat atgaaacat tgcggctat ttgtatgcc ttggggaaa aacgtttgg aaaaagtgg aaaaagcga tattacgtt ctggtgcaa gtctcccag tacacattt gcaatgtgc agttacaag gaaaaaaag tcggagcct tccgagatg atgcagttg gacggctac acggtcgac tacatcgaa gcctcgagt gccaactta atggtcgga atgggtaag catttggaa ggcgggcgg tactttttt aacgccgtc aaggagggc gatagcatt atttacgcg agcgacgac gagaacgag tgccatttg tgggtgatg gcaatgtac cgcgcgacc ggccaaagc cacaagccc accccgccc ctgactttg caagacaaa aactccacc atctcgaaa attcaagga gacgctgat cgggcccga aagcacggc atggaagac ttcatatcg gccgatccg tgccagttc gaccacgcg gctctgttc aagctcctg cagaacctc acgttagag taccgactc aacgacccg tactgctct ctggggtgg tttagccct ggccaagtg tttgttttg gacgagtat tgtgcgaga tacggagtg cggggatgt tacagacat ttgtgttat ttatctgac ttgttagat agggccgaa aaaaatatt atgattgat cctaccctt atacactat agttttgcg ttctgtgcc agccacgtc catggaaac agacccgac ggcgttgga agcgtcacc cacgaagag aaagaaaaa tttcaagaa gttaaggaa cggttacga gttttgtta gaaaaccaa attaccaat ttcaggtat tgttttcct tttgggaga ccagagggc gccttaaaa gcaactctt tcattatta gaaagagtg ctaatgaaa gatatcgtt acaccagtg cctcctgaa gaagtacgc gggatgatt aagaaatgt ttagaaact gctgcgtta ctcaactac actagatta tcttcagaa gcaaaaata gaagaagat ttgcgaggg gaaaccatg gtgccaccc agcaaaaaa ttagacgat ttaattcac ttagctgag ttatgtgtt gacttattg caacaaaac gaggaacat tatgccgag gcctttgcg tggttcagt gatttatta gtcgaacac gcggaaata ttttggtcc ttgtttgcc gtcgacatg gaccgcgtc ctagctgag caaccagca gacacgtgg gactcgttc ccacttttc caaatcctt aacgattac ctccgaact gatgacaac ttgaaaaat gggcggttc caccaacat ttaagagac acttttgcg cctctggtc gtacgttat gtcgatcta atggagtct tcgatcgcc cagtcgata cacaagggc ttcgagaag gaacgatgg gagatcaag ggcaatggg tgtgccacc tccgaggac ttgttctgg aaactggac gcgctccag tcgttcatc cgcgacctc cactggccg gacgccgag ttccggcaa cacctggaa cagcgtttg aagctgatg gcgtgcgat atgatcgag tcgtgcatc caacggacg gagaccgcg ttccagcag tggctgaag aagtccgtc actttcatc tccaccgac tacatcatc ccgtcggag atgtgtgcc atggttaac gttatcctg gacgctaag aaccagtcg ttgaagttg tgtgctgtc gacggaatc gacgttcac caataccac acgaaaatc gacgaccaa atcgacaaa accctggcg aacatgaac caggggatg atcagcaag ctgatgtcg gtcctggaa gccacactg tcgaagcta tcgcgctac gacgagggg agtttgatc ggctcgatt ctgagcttt acgaacgtg tccgggtct ggcaaggac atgggccag ggttatgtg aatttcacg cggaactgc atggaccag attaggggt aaagtgacg gacgaactc tggattcta aatttcttc gaacagtgg tacaccagc caaatcaac atgctttgc aactggtta tcagaacgg ttggatcac actttgcat ccataccaa tgtacgtgc cttgcccat attgtgaag aaaatctac tccgatttc gaacttcaa ggcgtgatg gaagataag ttgaattcc aaagcttac caaaccata gcccagcga atgcagacc gaggaggca acgtgtgcc cttaccatg ggacaggct gaagggggg agtgagggt ttggacgaa gacggtgat aatcgaagt aagacgaaa gttacgatc atggagagt ctgagtggc gaagacggg aattatgtc tcgaacatc gggaatatg gtggcagga cgggtcggg aatatcttc gggaagggg ttgggagga atcagtaca aaatttggt ggtggaaca agcagttgg ttttaa
- >TC013590 RB
atgactgta cctaaaaac ctccccgat caagactta cggattaga atagcgtgt agaatggac aagcctttg aatatgaaa cattgcggc tatttgtat gccttgggg aaaaacgtt tggaaaaag tggaaaaag cgatattac gttctggtg caagtctcc cagtacaca tttgcaatg tgcagttac aaggaaaaa aagtcggag ccttccgag atgatgcag ttggacggc tacacggtc gactacatc gaagcctcg agtgccaac ttaatggtc ggaatgggt aagcatttg gaaggcggg cggtacttt tttaacgcc gtcaaggag ggcgatagc attatttac gcgagcgac gacgagaac gagtgccat ttgtgggtg atggcaatg taccgcgcg accggccaa agccacaag cccaccccg cccctgact ttgcaagac aaaaactcc accatctcg aaaattcaa ggagacgct gatcgggcc cgaaagcac ggcatggaa gacttcata tcggccgat ccgtgccag ttcgaccac gcggctctg ttcaagctc ctgcagaac ctcacgtta gagtaccga ctcaacgac ccgtactgc tctctgggg tggtttagc cctggccaa gtgtttgtt ttggacgag tattgtgcg agatacgga gtgcgggga tgttacaga catttgtgt tatttatct gacttgtta gatagggcc gaaaaaaat attatgatt gatcctacc cttatacac tatagtttt gcgttctgt gccagccac gtccatgga aacagaccc gacggcgtt ggaagcgtc acccacgaa gagaaagaa aaatttcaa gaagttaag gaacggtta cgagttttg ttagaaaac caaattacc aatttcagg tattgtttt ccttttggg agaccagag ggcgcctta aaagcaact ctttcatta ttagaaaga gtgctaatg aaagatatc gttacacca gtgcctcct gaagaagta cgcgggatg attaagaaa tgtttagaa actgctgcg ttactcaac tacactaga ttatcttca gaagcaaaa atagaagaa gatttgcga ggggaaacc atggtgcca cccagcaaa aaattagac gatttaatt cacttagct gagttatgt gttgactta ttgcaacaa aacgaggaa cattatgcc gaggccttt gcgtggttc agtgattta ttagtcgaa cacgcggaa atattttgg tccttgttt gccgtcgac atggaccgc gtcctagct gagcaacca gcagacacg tgggactcg ttcccactt ttccaaatc cttaacgat tacctccga actgatgac aacttgaaa aatgggcgg ttccaccaa catttaaga gacactttt gcgcctctg gtcgtacgt tatgtcgat ctaatggag tcttcgatc gcccagtcg atacacaag ggcttcgag aaggaacga tgggagatc aagggcaat gggtgtgcc acctccgag gacttgttc tggaaactg gacgcgctc cagtcgttc atccgcgac ctccactgg ccggacgcc gagttccgg caacacctg gaacagcgt ttgaagctg atggcgtgc gatatgatc gagtcgtgc atccaacgg acggagacc gcgttccag cagtggctg aagaagtcc gtcactttc atctccacc gactacatc atcccgtcg gagatgtgt gccatggtt aacgttatc ctggacgct aagaaccag tcgttgaag ttgtgtgct gtcgacgga atcgacgtt caccaatac cacacgaaa atcgacgac caaatcgac aaaaccctg gcgaacatg aaccagggg atgatcagc aagctgatg tcggtcctg gaagccaca ctgtcgaag ctatcgcgc tacgacgag gggagtttg atcggctcg attctgagc tttacgaac gtgtccggg tctggcaag gacatgggc cagggttat gtgaatttc acgcggaac tgcatggac cagattagg ggtaaagtg acggacgaa ctctggatt ctaaatttc ttcgaacag tggtacacc agccaaatc aacatgctt tgcaactgg ttatcagaa cggttggat cacactttg catccatac caatgtacg tgccttgcc catattgtg aagaaaatc tactccgat ttcgaactt caaggcgtg atggaagat aagttgaat tccaaagct taccaaacc atagcccag cgaatgcag accgaggag gcaacgtgt gcccttacc atgggacag gctgaaggg gggagtgag ggtttggac gaagacggt gataatcga agtaagacg aaagttacg atcatggag agtctgagt ggcgaagac gggaattat gtctcgaac atcgggaat atggtggca ggacgggtc gggaatatc ttcgggaag gggttggga ggaatcagt acaaaattt ggtggtgga acaagcagt tggttttaa
- >TC013590 RC
atgactgta cctaaaaac ctccccgat caagactta cggattaga atagcgtgt agaatggac aagcctttg aatatgaaa cattgcggc tatttgtat gccttgggg aaaaacgtt tggaaaaag tggaaaaag cgatattac gttctggtg caagtctcc cagtacaca tttgcaatg tgcagttac aaggaaaaa aagtcggag ccttccgag atgatgcag ttggacggc tacacggtc gactacatc gaagcctcg agtgccaac ttaatggtc ggaatggat ttggaaggc gggcggtac ttttttaac gccgtcaag gagggcgat agcattatt tacgcgagc gacgacgag aacgagtgc catttgtgg gtgatggca atgtaccgc gcgaccggc caaagccac aagcccacc ccgcccctg actttgcaa gacaaaaac tccaccatc tcgaaaatt caaggagac gctgatcgg gcccgaaag cacggcatg gaagacttc atatcggcc gatccgtgc cagttcgac cacgcggct ctgttcaag ctcctgcag aacctcacg ttagagtac cgactcaac gacccgtac tgctctctg gggtggttt agccctggc caagtgttt gttttggac gagtattgt gcgagatac ggagtgcgg ggatgttac agacatttg tgttattta tctgacttg ttagatagg gccgaaaaa aatattatg attgatcct acccttata cactatagt tttgcgttc tgtgccagc cacgtccat ggaaacaga cccgacggc gttggaagc gtcacccac gaagagaaa gaaaaattt caagaagtt aaggaacgg ttacgagtt ttgttagaa aaccaaatt accaatttc aggtattgt tttcctttt gggagacca gagggcgcc ttaaaagca actctttca ttattagaa agagtgcta atgaaagat atcgttaca ccagtgcct cctgaagaa gtacgcggg atgattaag aaatgttta gaaactgct gcgttactc aactacact agattatct tcagaagca aaaatagaa gaagatttg cgaggggaa accatggtg ccacccagc aaaaaatta gacgattta attcactta gctgagtta tgtgttgac ttattgcaa caaaacgag gaacattat gccgaggcc tttgcgtgg ttcagtgat ttattagtc gaacacgcg gaaatattt tggtccttg tttgccgtc gacatggac cgcgtccta gctgagcaa ccagcagac acgtgggac tcgttccca cttttccaa atccttaac gattacctc cgaactgat gacaacttg aaaaatggg cggttccac caacattta agagacact tttgcgcct ctggtcgta cgttatgtc gatctaatg gagtcttcg atcgcccag tcgatacac aagggcttc gagaaggaa cgatgggag atcaagggc aatgggtgt gccacctcc gaggacttg ttctggaaa ctggacgcg ctccagtcg ttcatccgc gacctccac tggccggac gccgagttc cggcaacac ctggaacag cgtttgaag ctgatggcg tgcgatatg atcgagtcg tgcatccaa cggacggag accgcgttc cagcagtgg ctgaagaag tccgtcact ttcatctcc accgactac atcatcccg tcggagatg tgtgccatg gttaacgtt atcctggac gctaagaac cagtcgttg aagttgtgt gctgtcgac ggaatcgac gttcaccaa taccacacg aaaatcgac gaccaaatc gacaaaacc ctggcgaac atgaaccag gggatgatc agcaagctg atgtcggtc ctggaagcc acactgtcg aagctatcg cgctacgac gaggggagt ttgatcggc tcgattctg agctttacg aacgtgtcc gggtctggc aaggacatg ggccagggt tatgtgaat ttcacgcgg aactgcatg gaccagatt aggggtaaa gtgacggac gaactctgg attctaaat ttcttcgaa cagtggtac accagccaa atcaacatg ctttgcaac tggttatca gaacggttg gatcacact ttgcatcca taccaatgt acgtgcctt gcccatatt gtgaagaaa atctactcc gatttcgaa cttcaaggc gtgatggaa gataagttg aattccaaa gcttaccaa accatagcc cagcgaatg cagaccgag gaggcaacg tgtgccctt accatggga caggctgaa ggggggagt gagggtttg gacgaagac ggtgataat cgaagtaag acgaaagtt acgatcatg gagagtctg agtggcgaa gacgggaat tatgtctcg aacatcggg aatatggtg gcaggacgg gtcgggaat atcttcggg aaggggttg ggaggaatc agtacaaaa tttggtggt ggaacaagc agttggttt taa
- >TC013590 RD
atgattgat ccatcttca tctgaagaa gacagcgaa gaggagcat gcccctcct cacgcgggc caccatcct gccgcccac caccaccac caccaaacc acccaccac caccaccat ccctccgaa gcttctcct taccatgct cacttgtca gattctggt agtgggaac ctggaggtt ccgaacaac acagcggcc ctccaacgt tccttatca ccatccagt gccgtatca gccgaaacc cttagccac agtatagga tcctcccgc gcgaactcc ctcccccgt cccacgagt ccctccccg tccgtggcc tcggaaaaa accgaagtt gacctccag gagaagcag gaacgcgag gaggaagag cgaaaaagg cgcattcag ctttacgtg ttcatttcg aggtgtatc gcttatccc ttcaacgca aaacaaccg acggatatg acacgacgt cagaccaaa atcacaaaa caccaatta gaaactatc accgccaga tttcagagt tttttgaaa ggagaaact caaataatg gcggatgaa gctttccaa aacgctgta caaaattac tacgatgtt ttcctcaaa tcggaaagg gtagtcaaa atggtgcaa tctggtgct tgttcgcaa tatgatttt agagaggtt tttaggaat aatatcgag aaacgcgtt aggtctttg ccagaaatg gaaggttta agtaaagaa accgtattt acctcatgg atggcgaaa tttgactgt attctaaaa ggattaggc gacgaagat tccaagcgg ccttctaga atgcaacag caatcttta aattcggag ttaatttta tcgaaagaa cagctgtac gacatgttc caacaaata ctgggagtc aaaaaattc gaacaccaa cttcttttc aatgctcta atgcttgat tcggctgac gaacaagcc gctgctatt cggagagag ttagatggc agaatgcaa aaagtagct gaaatggaa aagaaccgg aagctaatg ccgaaattt gtactgaaa gaaatggag tctttgtac atagaggag ttaaagtca tcaataaat ttattgatg gcaaatcta gaatctctc cccgtttca aaaggatcg atggactct aaatatggc ttgcaaaaa ctaaaacgc tataatcac aggttgcat aaccataat aatccgtat aatgtgaag catcttttg atgaattat gttacttct tttagatcg caaggatct ttagcgaaa ttagaagga gactcagct gacagtgac acacaactg acaaaactt gatgttgtc ctaactttt cagttagag gtagtagta atggaagta aaaggttta aaatctcta gcgccaaat cgaatcgtg tattgcacc atggaggtc gagaacgga gaaaagctc caaactgac caggccgaa gcctcgaaa ccgatgtgg gacacgcag ggcgatttt tgcacgacc caccctttg ccaattgtc aaagtaaaa ctgtacacc gaaaacccc ggaatgcta gccctcgaa gacaaagaa ctcgggaaa gtcatcctc aaaccgacg cccctctcg tcaaaggta tga
- >TC013590 RE
atgattgat ccatcttca tctgaagaa gacagcgaa gaggagcat gcccctcct cacgcgggc caccatcct gccgcccac caccaccac caccaaacc acccaccac caccaccat ccctccgaa gcttctcct taccatgct cacttgtca gattctggt agtgggaac ctggaggtt ccgaacaac acagcggcc ctccaacgt tccttatca ccatccagt gccgtatca gccgaaacc cttagccac agtatagga tcctcccgc gcgaactcc ctcccccgt cccacgagt ccctccccg tccgtggcc tcggaaaaa accgaagtt gacctccag gagaagcag gaacgcgag gaggaagag cgaaaaagg cgcattcag ctttacgtg ttcatttcg aggtgtatc gcttatccc ttcaacgca aaacaaccg acggatatg acacgacgt cagaccaaa atcacaaaa caccaatta gaaactatc accgccaga tttcagagt tttttgaaa ggagaaact caaataatg gcggatgaa gctttccaa aacgctgta caaaattac tacgatgtt ttcctcaaa tcggaaagg gtagtcaaa atggtgcaa tctggtgct tgttcgcaa tatgatttt agagaggtt tttaggaat aatatcgag aaacgcgtt aggtctttg ccagaaatg gaaggttta agtaaagaa accgtattt acctcatgg atggcgaaa tttgactgt attctaaaa ggattaggc gacgaagat tccaagcgg ccttctaga atgcaacag caatcttta aattcggag ttaatttta tcgaaagaa cagctgtac gacatgttc caacaaata ctgggagtc aaaaaattc gaacaccaa cttcttttc aatgctcta atgcttgat tcggctgac gaacaagcc gctgctatt cggagagag ttagatggc agaatgcaa aaagtagct gaaatggaa aagaaccgg aagctaatg ccgaaattt gtactgaaa gaaatggag tctttgtac atagaggag ttaaagtca tcaataaat ttattgatg gcaaatcta gaatctctc cccgtttca aaaggatcg atggactct aaatatggc ttgcaaaaa ctaaaacgc tataatcac agatcgcaa ggatcttta gcgaaatta gaaggagac tcagctgac agtgacaca caactgaca aaacttgat gttgtccta acttttcag ttagaggta attttcgcc cattgctcc acgtccata tttcattca cgctctgta ctctcttcc aatctcatt gacagccct ctcctctca cgtattatt aagacgttt tcagttctt atgccactt caagatctc gtgcacttg tttccgatt ttattgact tgctacgtc ttacacatt cgtccgccc tctctctaa
- >TC013590 RF
atgattgat ccatcttca tctgaagaa gacagcgaa gaggagcat gcccctcct cacgcgggc caccatcct gccgcccac caccaccac caccaaacc acccaccac caccaccat ccctccgaa gcttctcct taccatgct cacttgtca gattctggt agtgggaac ctggaggtt ccgaacaac acagcggcc ctccaacgt tccttatca ccatccagt gccgtatca gccgaaacc cttagccac agtatagga tcctcccgc gcgaactcc ctcccccgt cccacgagt ccctccccg tccgtggcc tcggaaaaa accgaagtt gacctccag gagaagcag gaacgcgag gaggaagag cgaaaaagg cgcattcag ctttacgtg ttcatttcg aggtgtatc gcttatccc ttcaacgca aaacaaccg acggatatg acacgacgt cagaccaaa atcacaaaa caccaatta gaaactatc accgccaga tttcagagt tttttgaaa ggagaaact caaataatg gcggatgaa gctttccaa aacgctgta caaaattac tacgatgtt ttcctcaaa tcggaaagg gtagtcaaa atggtgcaa tctggtgct tgttcgcaa tatgatttt agagaggtt tttaggaat aatatcgag aaacgcgtt aggtctttg ccagaaatg gaaggttta agtaaagaa accgtattt acctcatgg atggcgaaa tttgactgt attctaaaa ggattaggc gacgaagat tccaagcgg ccttctaga atgcaacag caatcttta aattcggag ttaatttta tcgaaagaa cagctgtac gacatgttc caacaaata ctgggagtc aaaaaattc gaacaccaa cttcttttc aatgctcta atgcttgat tcggctgac gaacaagcc gctgctatt cggagagag ttagatggc agaatgcaa aaagtagct gaaatggaa aagaaccgg aagctaatg ccgaaattt gtactgaaa gaaatggag tctttgtac atagaggag ttaaagtca tcaataaat ttattgatg gcaaatcta gaatctctc cccgtttca aaaggatcg atggactct aaatatggc ttgcaaaaa ctaaaacgc tataatcac aggttgcat aaccataat aatccgtat aatgtgaag catcttttg atgaattat gttacttct tttagatcg caaggatct ttagcgaaa ttagaagga gactcagct gacagtgac acacaactg acaaaactt gatgttgtc ctaactttt cagttagag gtaattttc gcccattgc tccacgtcc atatttcat tcacgctct gtactctct tccaatctc attgacagc cctctcctc tcacgtatt attaagacg ttttcagtt cttatgcca cttcaagat ctcgtgcac ttgtttccg attttattg acttgctac gtcttacac attcgtccg ccctctctc taa
- >TC013590 RA
caaagtcag tgtgcgcgc cataacata attgtcttt agctggagg ctctccaaa cacgttctc tagagtcgc tattttagg tagtagtaa tggaagtaa aaggtttaa aatctctag cgccaaatc gaatcgtgt attgcacca tggaggtcg agaacggag aaaagctcc aaactgacc aggccgaag cctcgaaac cgatgtggg acacgcagg gcgattttt gcacgaccc accctttgc caattgtca aagtaaaac tgtacaccg aaaaccccg gaatgctag ccctcgaag acaaagaac tcgggaaag tcatcctca aaccgacgc ccctctcgt caaaggtac cggaatggc acaaaatga ctgtaccta aaaacctcc ccgatcaag acttacgga ttagaatag cgtgtagaa tggacaagc ctttgaata tgaaacatt gcggctatt tgtatgcct tggggaaaa acgtttgga aaaagtgga aaaagcgat attacgttc tggtgcaag tctcccagt acacatttg caatgtgca gttacaagg aaaaaaagt cggagcctt ccgagatga tgcagttgg acggctaca cggtcgact acatcgaag cctcgagtg ccaacttaa tggtcggaa tgggtaagc atttggaag gcgggcggt actttttta acgccgtca aggagggcg atagcatta tttacgcga gcgacgacg agaacgagt gccatttgt gggtgatgg caatgtacc gcgcgaccg gccaaagcc acaagccca ccccgcccc tgactttgc aagacaaaa actccacca tctcgaaaa ttcaaggag acgctgatc gggcccgaa agcacggca tggaagact tcatatcgg ccgatccgt gccagttcg accacgcgg ctctgttca agctcctgc agaacctca cgttagagt accgactca acgacccgt actgctctc tggggtggt ttagccctg gccaagtgt ttgttttgg acgagtatt gtgcgagat acggagtgc ggggatgtt acagacatt tgtgttatt tatctgact tgttagata gggccgaaa aaaatatta tgattgatc ctaccctta tacactata gttttgcgt tctgtgcca gccacgtcc atggaaaca gacccgacg gcgttggaa gcgtcaccc acgaagaga aagaaaaat ttcaagaag ttaaggaac ggttacgag ttttgttag aaaaccaaa ttaccaatt tcaggtatt gttttcctt ttgggagac cagagggcg ccttaaaag caactcttt cattattag aaagagtgc taatgaaag atatcgtta caccagtgc ctcctgaag aagtacgcg ggatgatta agaaatgtt tagaaactg ctgcgttac tcaactaca ctagattat cttcagaag caaaaatag aagaagatt tgcgagggg aaaccatgg tgccaccca gcaaaaaat tagacgatt taattcact tagctgagt tatgtgttg acttattgc aacaaaacg aggaacatt atgccgagg cctttgcgt ggttcagtg atttattag tcgaacacg cggaaatat tttggtcct tgtttgccg tcgacatgg accgcgtcc tagctgagc aaccagcag acacgtggg actcgttcc cacttttcc aaatcctta acgattacc tccgaactg atgacaact tgaaaaatg ggcggttcc accaacatt taagagaca cttttgcgc ctctggtcg tacgttatg tcgatctaa tggagtctt cgatcgccc agtcgatac acaagggct tcgagaagg aacgatggg agatcaagg gcaatgggt gtgccacct ccgaggact tgttctgga aactggacg cgctccagt cgttcatcc gcgacctcc actggccgg acgccgagt tccggcaac acctggaac agcgtttga agctgatgg cgtgcgata tgatcgagt cgtgcatcc aacggacgg agaccgcgt tccagcagt ggctgaaga agtccgtca ctttcatct ccaccgact acatcatcc cgtcggaga tgtgtgcca tggttaacg ttatcctgg acgctaaga accagtcgt tgaagttgt gtgctgtcg acggaatcg acgttcacc aataccaca cgaaaatcg acgaccaaa tcgacaaaa ccctggcga acatgaacc aggggatga tcagcaagc tgatgtcgg tcctggaag ccacactgt cgaagctat cgcgctacg acgagggga gtttgatcg gctcgattc tgagcttta cgaacgtgt ccgggtctg gcaaggaca tgggccagg gttatgtga atttcacgc ggaactgca tggaccaga ttaggggta aagtgacgg acgaactct ggattctaa atttcttcg aacagtggt acaccagcc aaatcaaca tgctttgca actggttat cagaacggt tggatcaca ctttgcatc cataccaat gtacgtgcc ttgcccata ttgtgaaga aaatctact ccgatttcg aacttcaag gcgtgatgg aagataagt tgaattcca aagcttacc aaaccatag cccagcgaa tgcagaccg aggaggcaa cgtgtgccc ttaccatgg gacaggctg aagggggga gtgagggtt tggacgaag acggtgata atcgaagta agacgaaag ttacgatca tggagagtc tgagtggcg aagacggga attatgtct cgaacatcg ggaatatgg tggcaggac gggtcggga atatcttcg ggaaggggt tgggaggaa tcagtacaa aatttggtg gtggaacaa gcagttggt tttaaacta gcagatttt attatagct tatgttttc tcacagagt tacaagaat gctggcact ggcctgata ttgtgatgg tccgctttt tatctagat gaataattt caaagcaat tttgtaccg tttgttgac atttgttga cgttctata ccaataata ttgttcgag aatacccag tactcagca ctgttcttt atttgccac cactatata tagttctaa tcgaatgat tttgttatc tgtgcctcg tggcgcgac atctataca ttaagcctt caggaacac accatttga attggtaca ataaatgac attcagtaa caactgcac tggactcga aacgtttaa ccattagct cataactta agtacaggc cacaaattg ctgtttgct agaccgtta ataaatttt tatcacata tcaagcact aaactgctc caatttatt ttacgtgcc gttaaaaac cgacactta atttgtatt ataaatatg agcgagtcc agtgatgtt ggtgagtgt agtataaag atcgcaatt acgacgttt tcagtatct gtagcacat tgcgcatat ctttaagaa tgcattgtc gtagacata caactggat actgaaaat gtttttgtt tttaaatat gtatttgct ttatgtgct gctggcatc ggtttgtaa agtacggtt acacggaga cagcttata catctctat aagttacta tgttgttca gttagattt tagaataaa ctgattaaa taacaacga taactaatt gaaattttt tatggtaac tgcatgccg ctgctgcta atattttta aaattctct gtagctctt ttatcagaa aacattgac gagtacaaa tttatcatt cgtatattg tagcataga tatcaacaa ttttgatgc gtaattttt gataaatgt gaataggtg acgatatag tcttaaatt aataactct tcatcactt cattatata ataaaaagg tatatccaa ttggttgtt gaaaacttt aaagtttaa aaatattcc catataagt gtatataga aatacagat gttcgtatt tcataatta ctttatctg ccttttcat gaacatcaa atactatac ttattgtag gatataact gaataatag acggtaact gaatttcat agtaacttg cttttttat ttccaaaat caataaaca cgttccaat acgacgtat acttgtcta tataaaaca gtctatgtt tttcgtttt tgttgcaaa acggtcatg gcaattctt agcactgat ttgtaaatg aatgttggg cctgtgatg tcgacaaac caaaagatg ctaaaaaat ttaagccta tataaatga ttatttata ggctagata atattgtta tgtttaact aacttgtac ttactgatg gtaaataaa ttgttttaa aagat
- >TC013590 RB
caaagtcag tgtgcgcgc cataacata attgtagta gtaatggaa gtaaaaggt ttaaaatct ctagcgcca aatcgaatc gtgtattgc accatggag gtcgagaac ggagaaaag ctccaaact gaccaggcc gaagcctcg aaaccgatg tgggacacg cagggcgat ttttgcacg acccaccct ttgccaatt gtcaaagta aaactgtac accgaaaac cccggaatg ctagccctc gaagacaaa gaactcggg aaagtcatc ctcaaaccg acgcccctc tcgtcaaag gtatgaaaa tcgaacgcc tctccaaac acttggctc gatgtttca ctcaaccca acaaatgca cgccaaacc acttaaact gcgttctaa ataaatcat cggcgcgca tttgcatac gaaagctgc ctttgcatt ttaaaggtg gttcaagtg catttgtcg ccgaaacac acaacttca cacattttg gaaaagtcg ggtgtgttg tagttctta gctacgtaa acgggtcag gttttggaa ccaacaatc gacactcac gaacaccac cacacgagg aacaaacga ccttgtgag atcccaaaa acaatttaa actcagtgt tggtagctg tgtttaaca gtccagaga tacttctcg aaaagtttg agtttagta gatgttggc agcaattga cgaaattca cccattcac ccatttatt gttagtctc cacaagcag cagctcaca ccgttattt gcactgact gcaaactgg ggtttaggt tggacagat gtaaaagcc atacacgat tttaactgt tatttaata accttgtta aattgttga ccgctcgct tgtgttcaa tcaaacgct cataaaaaa cgaattacg cgtaaaata aaacgaggg ttgttttaa ttttaaata ctttacacc tatttagcc taaactaat aatccgaca gcacgttta cagcacagt tacacaata ctagtcact tggacataa tttatttcg taagtgttt tatgcagtg tctactgaa acatgtgtc aaaagtgag aaaaaagtt aggcaattt ctttaatta atactacat cacggccca cttttccaa aacccacaa acatgcatg gcacatggt gtaaattaa tgatgtgtt cgccacaaa cagtacgtt tccccgata cttatgtac atgtactgt ggaacaata atttttgat tttagtgtt taacgcggc acatgtttt tgtgacaca ctgtatttg caactctgt ccactgtcc gtacttttc gttttcgaa ttatattta ggtcttata gttactaat aaagcgtta aaatgagtc aattgtggg caagatctg gtaaacgaa ggcttttcg tttctggga cccgtatta taatcgcct gaactcact taggtaccg gaatggcac aaaatgact gtacctaaa aacctcccc gatcaagac ttacggatt agaatagcg tgtagaatg gacaagcct ttgaatatg aaacattgc ggctatttg tatgccttg gggaaaaac gtttggaaa aagtggaaa aagcgatat tacgttctg gtgcaagtc tcccagtac acatttgca atgtgcagt tacaaggaa aaaaagtcg gagccttcc gagatgatg cagttggac ggctacacg gtcgactac atcgaagcc tcgagtgcc aacttaatg gtcggaatg ggtaagcat ttggaaggc gggcggtac ttttttaac gccgtcaag gagggcgat agcattatt tacgcgagc gacgacgag aacgagtgc catttgtgg gtgatggca atgtaccgc gcgaccggc caaagccac aagcccacc ccgcccctg actttgcaa gacaaaaac tccaccatc tcgaaaatt caaggagac gctgatcgg gcccgaaag cacggcatg gaagacttc atatcggcc gatccgtgc cagttcgac cacgcggct ctgttcaag ctcctgcag aacctcacg ttagagtac cgactcaac gacccgtac tgctctctg gggtggttt agccctggc caagtgttt gttttggac gagtattgt gcgagatac ggagtgcgg ggatgttac agacatttg tgttattta tctgacttg ttagatagg gccgaaaaa aatattatg attgatcct acccttata cactatagt tttgcgttc tgtgccagc cacgtccat ggaaacaga cccgacggc gttggaagc gtcacccac gaagagaaa gaaaaattt caagaagtt aaggaacgg ttacgagtt ttgttagaa aaccaaatt accaatttc aggtattgt tttcctttt gggagacca gagggcgcc ttaaaagca actctttca ttattagaa agagtgcta atgaaagat atcgttaca ccagtgcct cctgaagaa gtacgcggg atgattaag aaatgttta gaaactgct gcgttactc aactacact agattatct tcagaagca aaaatagaa gaagatttg cgaggggaa accatggtg ccacccagc aaaaaatta gacgattta attcactta gctgagtta tgtgttgac ttattgcaa caaaacgag gaacattat gccgaggcc tttgcgtgg ttcagtgat ttattagtc gaacacgcg gaaatattt tggtccttg tttgccgtc gacatggac cgcgtccta gctgagcaa ccagcagac acgtgggac tcgttccca cttttccaa atccttaac gattacctc cgaactgat gacaacttg aaaaatggg cggttccac caacattta agagacact tttgcgcct ctggtcgta cgttatgtc gatctaatg gagtcttcg atcgcccag tcgatacac aagggcttc gagaaggaa cgatgggag atcaagggc aatgggtgt gccacctcc gaggacttg ttctggaaa ctggacgcg ctccagtcg ttcatccgc gacctccac tggccggac gccgagttc cggcaacac ctggaacag cgtttgaag ctgatggcg tgcgatatg atcgagtcg tgcatccaa cggacggag accgcgttc cagcagtgg ctgaagaag tccgtcact ttcatctcc accgactac atcatcccg tcggagatg tgtgccatg gttaacgtt atcctggac gctaagaac cagtcgttg aagttgtgt gctgtcgac ggaatcgac gttcaccaa taccacacg aaaatcgac gaccaaatc gacaaaacc ctggcgaac atgaaccag gggatgatc agcaagctg atgtcggtc ctggaagcc acactgtcg aagctatcg cgctacgac gaggggagt ttgatcggc tcgattctg agctttacg aacgtgtcc gggtctggc aaggacatg ggccagggt tatgtgaat ttcacgcgg aactgcatg gaccagatt aggggtaaa gtgacggac gaactctgg attctaaat ttcttcgaa cagtggtac accagccaa atcaacatg ctttgcaac tggttatca gaacggttg gatcacact ttgcatcca taccaatgt acgtgcctt gcccatatt gtgaagaaa atctactcc gatttcgaa cttcaaggc gtgatggaa gataagttg aattccaaa gcttaccaa accatagcc cagcgaatg cagaccgag gaggcaacg tgtgccctt accatggga caggctgaa ggggggagt gagggtttg gacgaagac ggtgataat cgaagtaag acgaaagtt acgatcatg gagagtctg agtggcgaa gacgggaat tatgtctcg aacatcggg aatatggtg gcaggacgg gtcgggaat atcttcggg aaggggttg ggaggaatc agtacaaaa tttggtggt ggaacaagc agttggttt taaactagc agattttat tatagctta tgttttctc acagagtta caagaatgc tggcactgg cctgatatt gtgatggtc cgcttttta tctagatga ataatttca aagcaattt tgtaccgtt tgttgacat ttgttgacg ttctatacc aataatatt gttcgagaa tacccagta ctcagcact gttctttat ttgccacca ctatatata gttctaatc gaatgattt tgttatctg tgcctcgtg gcgcgacat ctatacatt aagccttca ggaacacac catttgaat tggtacaat aaatgacat tcagtaaca actgcactg gactcgaaa cgtttaacc attagctca taacttaag tacaggcca caaattgct gtttgctag accgttaat aaattttta tcacatatc aagcactaa actgctcca atttatttt acgtgccgt taaaaaccg acacttaat ttgtattat aaatatgag cgagtccag tgatgttgg tgagtgtag tataaagat cgcaattac gacgttttc agtatctgt agcacattg cgcatatct ttaagaatg cattgtcgt agacataca actggatac tgaaaatgt ttttgtttt taaatatgt atttgcttt atgtgctgc tggcatcgg tttgtaaag tacggttac acggagaca gcttataca tctctataa gttactatg ttgttcagt tagatttta gaataaact gattaaata acaacgata actaattga aatttttta tggtaactg catgccgct gctgctaat atttttaaa attctctgt agctctttt atcagaaaa cattgacga gtacaaatt tatcattcg tatattgta gcatagata tcaacaatt ttgatgcgt aatttttga taaatgtga ataggtgac gatatagtc ttaaattaa taactcttc atcacttca ttatataat aaaaaggta tatccaatt ggttgttga aaactttaa agtttaaaa atattccca tataagtgt atatagaaa tacagatgt tcgtatttc ataattact ttatctgcc ttttcatga acatcaaat actatactt attgtagga tataactga ataatagac ggtaactga atttcatag taacttgct tttttattt ccaaaatca ataaacacg ttccaatac gacgtatac ttgtctata taaaacagt ctatgtttt tcgtttttg ttgcaaaac ggtcatggc aattcttag cactgattt gtaaatgaa tgttgggcc tgtgatgtc gacaaacca aaagatgct aaaaaattt aagcctata taaatgatt atttatagg ctagataat attgttatg tttaactaa cttgtactt actgatggt aaataaatt gttttaaaa gat
- >TC013590 RC
caaagtcag tgtgcgcgc cataacata attgtagta gtaatggaa gtaaaaggt ttaaaatct ctagcgcca aatcgaatc gtgtattgc accatggag gtcgagaac ggagaaaag ctccaaact gaccaggcc gaagcctcg aaaccgatg tgggacacg cagggcgat ttttgcacg acccaccct ttgccaatt gtcaaagta aaactgtac accgaaaac cccggaatg ctagccctc gaagacaaa gaactcggg aaagtcatc ctcaaaccg acgcccctc tcgtcaaag gtatgaaaa tcgaacgcc tctccaaac acttggctc gatgtttca ctcaaccca acaaatgca cgccaaacc acttaaact gcgttctaa ataaatcat cggcgcgca tttgcatac gaaagctgc ctttgcatt ttaaaggtg gttcaagtg catttgtcg ccgaaacac acaacttca cacattttg gaaaagtcg ggtgtgttg tagttctta gctacgtaa acgggtcag gttttggaa ccaacaatc gacactcac gaacaccac cacacgagg aacaaacga ccttgtgag atcccaaaa acaatttaa actcagtgt tggtagctg tgtttaaca gtccagaga tacttctcg aaaagtttg agtttagta gatgttggc agcaattga cgaaattca cccattcac ccatttatt gttagtctc cacaagcag cagctcaca ccgttattt gcactgact gcaaactgg ggtttaggt tggacagat gtaaaagcc atacacgat tttaactgt tatttaata accttgtta aattgttga ccgctcgct tgtgttcaa tcaaacgct cataaaaaa cgaattacg cgtaaaata aaacgaggg ttgttttaa ttttaaata ctttacacc tatttagcc taaactaat aatccgaca gcacgttta cagcacagt tacacaata ctagtcact tggacataa tttatttcg taagtgttt tatgcagtg tctactgaa acatgtgtc aaaagtgag aaaaaagtt aggcaattt ctttaatta atactacat cacggccca cttttccaa aacccacaa acatgcatg gcacatggt gtaaattaa tgatgtgtt cgccacaaa cagtacgtt tccccgata cttatgtac atgtactgt ggaacaata atttttgat tttagtgtt taacgcggc acatgtttt tgtgacaca ctgtatttg caactctgt ccactgtcc gtacttttc gttttcgaa ttatattta ggtcttata gttactaat aaagcgtta aaatgagtc aattgtggg caagatctg gtaaacgaa ggcttttcg tttctggga cccgtatta taatcgcct gaactcact taggtaccg gaatggcac aaaatgact gtacctaaa aacctcccc gatcaagac ttacggatt agaatagcg tgtagaatg gacaagcct ttgaatatg aaacattgc ggctatttg tatgccttg gggaaaaac gtttggaaa aagtggaaa aagcgatat tacgttctg gtgcaagtc tcccagtac acatttgca atgtgcagt tacaaggaa aaaaagtcg gagccttcc gagatgatg cagttggac ggctacacg gtcgactac atcgaagcc tcgagtgcc aacttaatg gtcggaatg gatttggaa ggcgggcgg tactttttt aacgccgtc aaggagggc gatagcatt atttacgcg agcgacgac gagaacgag tgccatttg tgggtgatg gcaatgtac cgcgcgacc ggccaaagc cacaagccc accccgccc ctgactttg caagacaaa aactccacc atctcgaaa attcaagga gacgctgat cgggcccga aagcacggc atggaagac ttcatatcg gccgatccg tgccagttc gaccacgcg gctctgttc aagctcctg cagaacctc acgttagag taccgactc aacgacccg tactgctct ctggggtgg tttagccct ggccaagtg tttgttttg gacgagtat tgtgcgaga tacggagtg cggggatgt tacagacat ttgtgttat ttatctgac ttgttagat agggccgaa aaaaatatt atgattgat cctaccctt atacactat agttttgcg ttctgtgcc agccacgtc catggaaac agacccgac ggcgttgga agcgtcacc cacgaagag aaagaaaaa tttcaagaa gttaaggaa cggttacga gttttgtta gaaaaccaa attaccaat ttcaggtat tgttttcct tttgggaga ccagagggc gccttaaaa gcaactctt tcattatta gaaagagtg ctaatgaaa gatatcgtt acaccagtg cctcctgaa gaagtacgc gggatgatt aagaaatgt ttagaaact gctgcgtta ctcaactac actagatta tcttcagaa gcaaaaata gaagaagat ttgcgaggg gaaaccatg gtgccaccc agcaaaaaa ttagacgat ttaattcac ttagctgag ttatgtgtt gacttattg caacaaaac gaggaacat tatgccgag gcctttgcg tggttcagt gatttatta gtcgaacac gcggaaata ttttggtcc ttgtttgcc gtcgacatg gaccgcgtc ctagctgag caaccagca gacacgtgg gactcgttc ccacttttc caaatcctt aacgattac ctccgaact gatgacaac ttgaaaaat gggcggttc caccaacat ttaagagac acttttgcg cctctggtc gtacgttat gtcgatcta atggagtct tcgatcgcc cagtcgata cacaagggc ttcgagaag gaacgatgg gagatcaag ggcaatggg tgtgccacc tccgaggac ttgttctgg aaactggac gcgctccag tcgttcatc cgcgacctc cactggccg gacgccgag ttccggcaa cacctggaa cagcgtttg aagctgatg gcgtgcgat atgatcgag tcgtgcatc caacggacg gagaccgcg ttccagcag tggctgaag aagtccgtc actttcatc tccaccgac tacatcatc ccgtcggag atgtgtgcc atggttaac gttatcctg gacgctaag aaccagtcg ttgaagttg tgtgctgtc gacggaatc gacgttcac caataccac acgaaaatc gacgaccaa atcgacaaa accctggcg aacatgaac caggggatg atcagcaag ctgatgtcg gtcctggaa gccacactg tcgaagcta tcgcgctac gacgagggg agtttgatc ggctcgatt ctgagcttt acgaacgtg tccgggtct ggcaaggac atgggccag ggttatgtg aatttcacg cggaactgc atggaccag attaggggt aaagtgacg gacgaactc tggattcta aatttcttc gaacagtgg tacaccagc caaatcaac atgctttgc aactggtta tcagaacgg ttggatcac actttgcat ccataccaa tgtacgtgc cttgcccat attgtgaag aaaatctac tccgatttc gaacttcaa ggcgtgatg gaagataag ttgaattcc aaagcttac caaaccata gcccagcga atgcagacc gaggaggca acgtgtgcc cttaccatg ggacaggct gaagggggg agtgagggt ttggacgaa gacggtgat aatcgaagt aagacgaaa gttacgatc atggagagt ctgagtggc gaagacggg aattatgtc tcgaacatc gggaatatg gtggcagga cgggtcggg aatatcttc gggaagggg ttgggagga atcagtaca aaatttggt ggtggaaca agcagttgg ttttaaact agcagattt tattatagc ttatgtttt ctcacagag ttacaagaa tgctggcac tggcctgat attgtgatg gtccgcttt ttatctaga tgaataatt tcaaagcaa ttttgtacc gtttgttga catttgttg acgttctat accaataat attgttcga gaataccca gtactcagc actgttctt tatttgcca ccactatat atagttcta atcgaatga ttttgttat ctgtgcctc gtggcgcga catctatac attaagcct tcaggaaca caccatttg aattggtac aataaatga cattcagta acaactgca ctggactcg aaacgttta accattagc tcataactt aagtacagg ccacaaatt gctgtttgc tagaccgtt aataaattt ttatcacat atcaagcac taaactgct ccaatttat tttacgtgc cgttaaaaa ccgacactt aatttgtat tataaatat gagcgagtc cagtgatgt tggtgagtg tagtataaa gatcgcaat tacgacgtt ttcagtatc tgtagcaca ttgcgcata tctttaaga atgcattgt cgtagacat acaactgga tactgaaaa tgtttttgt ttttaaata tgtatttgc tttatgtgc tgctggcat cggtttgta aagtacggt tacacggag acagcttat acatctcta taagttact atgttgttc agttagatt ttagaataa actgattaa ataacaacg ataactaat tgaaatttt ttatggtaa ctgcatgcc gctgctgct aatattttt aaaattctc tgtagctct tttatcaga aaacattga cgagtacaa atttatcat tcgtatatt gtagcatag atatcaaca attttgatg cgtaatttt tgataaatg tgaataggt gacgatata gtcttaaat taataactc ttcatcact tcattatat aataaaaag gtatatcca attggttgt tgaaaactt taaagttta aaaatattc ccatataag tgtatatag aaatacaga tgttcgtat ttcataatt actttatct gccttttca tgaacatca aatactata cttattgta ggatataac tgaataata gacggtaac tgaatttca tagtaactt gctttttta tttccaaaa tcaataaac acgttccaa tacgacgta tacttgtct atataaaac agtctatgt ttttcgttt ttgttgcaa aacggtcat ggcaattct tagcactga tttgtaaat gaatgttgg gcctgtgat gtcgacaaa ccaaaagat gctaaaaaa tttaagcct atataaatg attatttat aggctagat aatattgtt atgtttaac taacttgta cttactgat ggtaaataa attgtttta aaagat
- >TC013590 RD
gtccaagag agacggccg caagtacgc aacctgcgc aacgcagat agtaggtac agtatactg aagactttt caggtcaca gttctccaa gctgcaacc aacgggaaa tcaataccg agctttttt agaagtacc taatgcccc acattgcaa gcacagtct tcgccgtcg tcgccgccg tgtgccgtc gttgttgct aactcgccg cctcctctc tggccaagg catgcctct gctggctga ggcagccta gtgccgatc tcgggacag tgtgctccg cagaatggg atagcgaca ccgaaacct gcacaactt cccaactgt actaatcag attggacga aagaagcaa ggaagatga ttgatccat cttcatctg aagaagaca gcgaagagg agcatgccc ctcctcacg cgggccacc atcctgccg cccaccacc accaccacc aaaccaccc accaccacc accatccct ccgaagctt ctccttacc atgctcact tgtcagatt ctggtagtg ggaacctgg aggttccga acaacacag cggccctcc aacgttcct tatcaccat ccagtgccg tatcagccg aaaccctta gccacagta taggatcct cccgcgcga actccctcc cccgtccca cgagtccct ccccgtccg tggcctcgg aaaaaaccg aagttgacc tccaggaga agcaggaac gcgaggagg aagagcgaa aaaggcgca ttcagcttt acgtgttca tttcgaggt gtatcgctt atcccttca acgcaaaac aaccgacgg atatgacac gacgtcaga ccaaaatca caaaacacc aattagaaa ctatcaccg ccagatttc agagttttt tgaaaggag aaactcaaa taatggcgg atgaagctt tccaaaacg ctgtacaaa attactacg atgttttcc tcaaatcgg aaagggtag tcaaaatgg tgcaatctg gtgcttgtt cgcaatatg attttagag aggttttta ggaataata tcgagaaac gcgttaggt ctttgccag aaatggaag gtttaagta aagaaaccg tatttacct catggatgg cgaaatttg actgtattc taaaaggat taggcgacg aagattcca agcggcctt ctagaatgc aacagcaat ctttaaatt cggagttaa ttttatcga aagaacagc tgtacgaca tgttccaac aaatactgg gagtcaaaa aattcgaac accaacttc ttttcaatg ctctaatgc ttgattcgg ctgacgaac aagccgctg ctattcgga gagagttag atggcagaa tgcaaaaag tagctgaaa tggaaaaga accggaagc taatgccga aatttgtac tgaaagaaa tggagtctt tgtacatag aggagttaa agtcatcaa taaatttat tgatggcaa atctagaat ctctccccg tttcaaaag gatcgatgg actctaaat atggcttgc aaaaactaa aacgctata atcacaggt tgcataacc ataataatc cgtataatg tgaagcatc ttttgatga attatgtta cttctttta gatcgcaag gatctttag cgaaattag aaggagact cagctgaca gtgacacac aactgacaa aacttgatg ttgtcctaa cttttcagt tagaggtag tagtaatgg aagtaaaag gtttaaaat ctctagcgc caaatcgaa tcgtgtatt gcaccatgg aggtcgaga acggagaaa agctccaaa ctgaccagg ccgaagcct cgaaaccga tgtgggaca cgcagggcg atttttgca cgacccacc ctttgccaa ttgtcaaag taaaactgt acaccgaaa accccggaa tgctagccc tcgaagaca aagaactcg ggaaagtca tcctcaaac cgacgcccc tctcgtcaa aggtatgaa aatcgaacg cctctccaa acacttggc tcgatgttt cactcaacc caacaaatg cacgccaaa ccacttaaa ctgcgttct aaataaatc atcggcgcg catttgcat acgaaagct gcctttgca ttttaaagg tggttcaag tgcatttgt cgccgaaac acacaactt cacacattt tggaaaagt cgggtgtgt tgtagttct tagctacgt aaacgggtc aggttttgg aaccaacaa tcgacactc acgaacacc accacacga ggaacaaac gaccttgtg agatcccaa aaacaattt aaactcagt gttggtagc tgtgtttaa cagtccaga gatacttct cgaaaagtt tgagtttag tagatgttg gcagcaatt gacgaaatt cacccattc acccattta ttgttagtc tccacaagc agcagctca caccgttat ttgcactga ctgcaaact ggggtttag gttggacag atgtaaaag ccatacacg attttaact gttatttaa taaccttgt taaattgtt gaccgctcg cttgtgttc aatcaaacg ctcataaaa aacgaatta cgcgtaaaa taaaacgag ggttgtttt aattttaaa tactttaca cctatttag cctaaacta ataatccga cagcacgtt tacagcaca gttacacaa tactagtca cttggacat aatttattt cgtaagtgt tttatgcag tgtctactg aaacatgtg tcaaaagtg agaaaaaag ttaggcaat ttctttaat taatactac atcacggcc cacttttcc aaaacccac aaacatgca tggcacatg gtgtaaatt aatgatgtg ttcgccaca aacagtacg tttccccga tacttatgt acatgtact gtggaacaa taatttttg attttagtg tttaacgcg gcacatgtt tttgtgaca cactgtatt tgcaactct gtccactgt ccgtacttt tcgttttcg aattatatt taggtctta tagttacta ataaagcgt taaaatgag t
- >TC013590 RE
gtccaagag agacggccg caagtacgc aacctgcgc aacgcagat agtaggtac agtatactg aagactttt caggtcaca gttctccaa gctgcaacc aacgggaaa tcaataccg agctttttt agaagtacc taatgcccc acattgcaa gcacagtct tcgccgtcg tcgccgccg tgtgccgtc gttgttgct aactcgccg cctcctctc tggccaagg catgcctct gctggctga ggcagccta gtgccgatc tcgggacag tgtgctccg cagaatggg atagcgaca ccgaaacct gcacaactt cccaactgt actaatcag attggacga aagaagcaa ggaagatga ttgatccat cttcatctg aagaagaca gcgaagagg agcatgccc ctcctcacg cgggccacc atcctgccg cccaccacc accaccacc aaaccaccc accaccacc accatccct ccgaagctt ctccttacc atgctcact tgtcagatt ctggtagtg ggaacctgg aggttccga acaacacag cggccctcc aacgttcct tatcaccat ccagtgccg tatcagccg aaaccctta gccacagta taggatcct cccgcgcga actccctcc cccgtccca cgagtccct ccccgtccg tggcctcgg aaaaaaccg aagttgacc tccaggaga agcaggaac gcgaggagg aagagcgaa aaaggcgca ttcagcttt acgtgttca tttcgaggt gtatcgctt atcccttca acgcaaaac aaccgacgg atatgacac gacgtcaga ccaaaatca caaaacacc aattagaaa ctatcaccg ccagatttc agagttttt tgaaaggag aaactcaaa taatggcgg atgaagctt tccaaaacg ctgtacaaa attactacg atgttttcc tcaaatcgg aaagggtag tcaaaatgg tgcaatctg gtgcttgtt cgcaatatg attttagag aggttttta ggaataata tcgagaaac gcgttaggt ctttgccag aaatggaag gtttaagta aagaaaccg tatttacct catggatgg cgaaatttg actgtattc taaaaggat taggcgacg aagattcca agcggcctt ctagaatgc aacagcaat ctttaaatt cggagttaa ttttatcga aagaacagc tgtacgaca tgttccaac aaatactgg gagtcaaaa aattcgaac accaacttc ttttcaatg ctctaatgc ttgattcgg ctgacgaac aagccgctg ctattcgga gagagttag atggcagaa tgcaaaaag tagctgaaa tggaaaaga accggaagc taatgccga aatttgtac tgaaagaaa tggagtctt tgtacatag aggagttaa agtcatcaa taaatttat tgatggcaa atctagaat ctctccccg tttcaaaag gatcgatgg actctaaat atggcttgc aaaaactaa aacgctata atcacagat cgcaaggat ctttagcga aattagaag gagactcag ctgacagtg acacacaac tgacaaaac ttgatgttg tcctaactt ttcagttag aggtaattt tcgcccatt gctccacgt ccatatttc attcacgct ctgtactct cttccaatc tcattgaca gccctctcc tctcacgta ttattaaga cgttttcag ttcttatgc cacttcaag atctcgtgc acttgtttc cgattttat tgacttgct acgtcttac acattcgtc cgccctctc tctaagctt gctttcata accagccca tcatcagtc gcaaataat aataataat aattgtgat atgttcaat agcttgagc acttggttt aaataatta agggacaag ttg
- >TC013590 RF
gtccaagag agacggccg caagtacgc aacctgcgc aacgcagat agtaggtac agtatactg aagactttt caggtcaca gttctccaa gctgcaacc aacgggaaa tcaataccg agctttttt agaagtacc taatgcccc acattgcaa gcacagtct tcgccgtcg tcgccgccg tgtgccgtc gttgttgct aactcgccg cctcctctc tggccaagg catgcctct gctggctga ggcagccta gtgccgatc tcgggacag tgtgctccg cagaatggg atagcgaca ccgaaacct gcacaactt cccaactgt actaatcag attggacga aagaagcaa ggaagatga ttgatccat cttcatctg aagaagaca gcgaagagg agcatgccc ctcctcacg cgggccacc atcctgccg cccaccacc accaccacc aaaccaccc accaccacc accatccct ccgaagctt ctccttacc atgctcact tgtcagatt ctggtagtg ggaacctgg aggttccga acaacacag cggccctcc aacgttcct tatcaccat ccagtgccg tatcagccg aaaccctta gccacagta taggatcct cccgcgcga actccctcc cccgtccca cgagtccct ccccgtccg tggcctcgg aaaaaaccg aagttgacc tccaggaga agcaggaac gcgaggagg aagagcgaa aaaggcgca ttcagcttt acgtgttca tttcgaggt gtatcgctt atcccttca acgcaaaac aaccgacgg atatgacac gacgtcaga ccaaaatca caaaacacc aattagaaa ctatcaccg ccagatttc agagttttt tgaaaggag aaactcaaa taatggcgg atgaagctt tccaaaacg ctgtacaaa attactacg atgttttcc tcaaatcgg aaagggtag tcaaaatgg tgcaatctg gtgcttgtt cgcaatatg attttagag aggttttta ggaataata tcgagaaac gcgttaggt ctttgccag aaatggaag gtttaagta aagaaaccg tatttacct catggatgg cgaaatttg actgtattc taaaaggat taggcgacg aagattcca agcggcctt ctagaatgc aacagcaat ctttaaatt cggagttaa ttttatcga aagaacagc tgtacgaca tgttccaac aaatactgg gagtcaaaa aattcgaac accaacttc ttttcaatg ctctaatgc ttgattcgg ctgacgaac aagccgctg ctattcgga gagagttag atggcagaa tgcaaaaag tagctgaaa tggaaaaga accggaagc taatgccga aatttgtac tgaaagaaa tggagtctt tgtacatag aggagttaa agtcatcaa taaatttat tgatggcaa atctagaat ctctccccg tttcaaaag gatcgatgg actctaaat atggcttgc aaaaactaa aacgctata atcacaggt tgcataacc ataataatc cgtataatg tgaagcatc ttttgatga attatgtta cttctttta gatcgcaag gatctttag cgaaattag aaggagact cagctgaca gtgacacac aactgacaa aacttgatg ttgtcctaa cttttcagt tagaggtaa ttttcgccc attgctcca cgtccatat ttcattcac gctctgtac tctcttcca atctcattg acagccctc tcctctcac gtattatta agacgtttt cagttctta tgccacttc aagatctcg tgcacttgt ttccgattt tattgactt gctacgtct tacacattc gtccgccct ctctctaag cttgctttc ataaccagc ccatcatca gtcgcaaat aataataat aataattgt gatatgttc aatagcttg agcacttgg tttaaataa ttaagggac aagttg
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