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Gene information
General information
TC013578 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3506275..3510572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013578
    iBeetle screen iB_05458

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013578 RA
      MEVAMPMSG TAIDCYEDM FKEITRKLY GEDGLPELT 
      GTERLPTNQ DRGLTPIDY DVDNPILKP EEHLTAFGL 
      AALMQNGFP TTGILNSTF QQHKTTATT TVEDKWTAN 
      EDALQWTQS KIATYNPSQ KLFRCAECD CVGYLPRVA 
      EHWLGTHSN LRAFQCPQC PYESAWARC VRMHLSRQH 
      NINTDEASD TLAKNNPIL QEITKYLQR LKNKLENNC 
      AKPSHINNS QILDEFQSS STSTNNHLN NNQVVATNS 
      DASSSSSTK RYTCTYCPY ATDRRDLFT RHENIHREE 
      KPFQCYVCQ KQFNRADHV KKHFLRMHR EHPYDLNRI 
      RRHPPKNAS GMSYYQKYN ANSTNQSPD SISSLANIN 
      SLSIPNGFN NISRNANNI RPGNTTDMK NGITNIKSG 
      NISKGNGKK KGEKRFTCC YCSWSGVDN WCLKRHMNT 
      HLKPFVCGL CDYKAARSE RLATHVLKV HNKRACGKC 
      SFLADDPAQ LTVHQQEHH PLEQRNRSG NNILRNNSF 
      NNSTATSSS SCSQSQTSL KPHELSAAA ALLQHSNRL 
      LQNDNKWKN HAPKQHGAA RLFNYMEAS DGSEPESES 
      RGAEDMSRL NLNHRIQSD FLEAGDVGS QADEEEGEE 
      MELPLFVCP TCGCEFEDD MSLETHQYT HRHSTTSPA 
      KSDNSKENV NPNLLKYRC CLCDFAFEN QSSVMAHMV 
      SHSSLSGAQ AKSTLTKRS RKQSRPKKM VLPFWDLNE 
      VEVLRHLNG NLSGELQQA SASMSRKYM ARIVARKGL 
      YCVLCKSSS VNRYHTKAS LALHHYWKH LNKRFKCEH 
      CDLHFRHRY QCVLHASRE HINKPPILK SQTVPIKEP 
      IHSAVPASQ DAHHFFANS QTVTVPDAS SNAHKIGMS 
      FFDHSFIHP TNAINSHMP IIIPTFPPN
    CDS sequence
    • >TC013578 RA
      atggaggtg gcaatgccc atgtcgggt actgccatc 
      gactgctat gaagacatg ttcaaagag ataaccagg 
      aagctctac ggcgaggac ggcttgcct gaattaacc 
      ggaaccgag cgacttccc acaaaccag gatcgtggc 
      ttaactccg atcgattat gacgttgac aatccaatt 
      ttaaagccg gaagaacat ttaactgca tttggctta 
      gctgcgcta atgcaaaat ggtttcccc acaacagga 
      atactcaat tcgacgttt caacaacac aagactact 
      gcaacaacg accgttgag gacaagtgg accgccaat 
      gaagacgct ctccagtgg acgcagtcg aaaattgcc 
      acttataat ccatcacag aaattgttt cgatgcgcg 
      gaatgtgat tgtgtcggg tatctcccg agagttgct 
      gaacattgg ttagggact cactcgaat ttaagagct 
      tttcaatgt ccgcaatgt ccgtacgaa agtgcgtgg 
      gccaggtgt gtacgaatg catttgagt cgccaacac 
      aacatcaac accgacgaa gcttcggac actttggcc 
      aaaaacaac ccaattttg caagaaatc acaaaatac 
      ctccaaagg ctcaaaaat aaattagaa aacaattgt 
      gcgaaacca tcacatata aacaactcg caaatctta 
      gacgagttt caatcttcc tcgacctct accaacaat 
      catctcaac aacaatcaa gtggttgcc acaaactcc 
      gacgcatcc agcagttcc tccaccaaa aggtacacc 
      tgtacctac tgcccctac gctacggac cgcagagac 
      ctatttacg cggcatgag aatattcat cgcgaagag 
      aaaccgttc cagtgttac gtctgtcag aaacaattc 
      aatcgagcg gatcatgtt aaaaagcac ttccttcga 
      atgcacagg gagcaccct tatgatctg aaccggata 
      cgccgacat cctccgaaa aacgcttcg ggaatgtcg 
      tattatcaa aaatacaac gcaaattct acaaatcaa 
      tcaccggat tccatatct tcgctggcg aatattaat 
      tccctttcc attccaaac ggatttaat aacatatcg 
      cgcaatgcc aataatatc agaccaggt aacaccacc 
      gatatgaag aacgggatt acaaacatt aaatctggg 
      aatatttcc aaaggtaat ggaaaaaag aaaggcgag 
      aaacgattc acttgttgt tactgctcc tggtcgggg 
      gttgataac tggtgctta aaacggcac atgaacact 
      catttaaaa ccattcgtg tgcggcctt tgtgattac 
      aaagcagcg agatctgaa agattggca acccacgtg 
      ctgaaagta cacaataaa cgagcttgt ggaaaatgc 
      agttttttg gccgacgat cccgcccaa ttaaccgta 
      caccagcaa gagcaccat cctttggag caacgaaac 
      cgatcagga aacaacatt ttgcgaaat aattccttc 
      aataacagc acggcaacg tcatcatcc tcgtgcagc 
      caaagccag acgagtctg aagccccac gagctgagt 
      gccgctgct gctctgctc cagcacagc aaccgcttg 
      cttcaaaac gacaacaag tggaaaaac cacgctccc 
      aagcaacac ggagctgcg cgtctcttc aattacatg 
      gaagccagc gatgggtcg gagccggag tcggaatca 
      cgcggggcc gaagacatg agtcggttg aacttgaat 
      caccggatt cagtcggat tttcttgag gcgggagat 
      gttggtagc caggccgac gaggaagag ggtgaagag 
      atggagctc cctttgttc gtctgcccc acgtgcggc 
      tgcgagttc gaggacgac atgtcactt gaaacccat 
      caatacacg caccgtcac agcaccaca tctcccgca 
      aaatccgac aactccaag gagaatgtc aaccctaat 
      ttactaaag tatcgatgt tgtttgtgc gattttgcc 
      tttgagaat cagtcttct gttatggcg cacatggtg 
      tctcattcg agcctgagc ggggcgcag gcgaagtcc 
      acgttgacg aagcgctcc cggaaacag agccgcccc 
      aaaaagatg gtcttgccg ttctgggac ttgaacgaa 
      gttgaagtg ttgcggcac ctcaatggg aacttaagc 
      ggcgagctg caacaggcc tctgcctcc atgtccagg 
      aagtacatg gccaggatt gtcgctcgc aaaggactg 
      tattgcgtg ctctgcaag agctccagt gtgaacaga 
      taccacaca aaggcgagc ctagctttg catcactac 
      tggaaacac ttgaacaag cgcttcaag tgcgaacat 
      tgcgaccta catttccgg catcggtat cagtgtgtg 
      ctgcatgcc agtcgcgag catataaat aaaccaccg 
      atccttaag tctcaaaca gtgcctatt aaagaacca 
      attcattcc gcagtacct gcttcccag gacgcccat 
      catttcttc gcaaactct caaactgtt actgtacct 
      gacgcatca tccaatgcg cataaaata gggatgtcg 
      ttttttgac cactcgttc atacacccc accaacgcc 
      atcaacagt catatgccg atcatcata cccactttt 
      ccaccaaat taa
    mRNA sequence
    • >TC013578 RA
      tcaattgag tgtgtttaa gttgatttt tgtgtgatc 
      atgtttgtt gtccatcgc aggtgtggg cgtaattta 
      aaggtgagt ttgtagaaa ataaatgtt cgtcgagtt 
      ccggcacat cggcgttct tttgagtcc gttcagcct 
      tcggtgagc tcaccctcc cgtaattct ggttgcttg 
      gcaacccct gccagccac cccttaaac ttccccgcg 
      agagacgaa ccccctcgc gcaatagtt tttatatga 
      tagctccga tggctttct tctgtggat ggcgaagct 
      tgcgccctc cgccaccct ccgactcga gcccttaat 
      tctgggatg catcggccg gcttcggag cattattag 
      ggcatgtag gagttgggg cgtcggaat tcgtgcatc 
      ccgtgtccc tcgcgctag agccttgtg tgcactgtg 
      tacagtagg gtgagatct ggaacggcc gcgaaggtg 
      gttgtgggc cgggcgggg tggccgggg tggtcgggg 
      tcgggacgg ggagcgata gtggggtgg catgggggg 
      ggtagggcc gggggcgga gggccccgg gcagcgggt 
      gccgcagtg ttgggtggg tcagatatc gcagaagca 
      gacgtccca tgcgttcgt tacgctctc catagtact 
      agtggttgg tgacatgca gtgatcggt gctttcgtt 
      tcgacggga caagctgac atgctgccc tttgcccac 
      acacaactg gaagacggg cctcagcag tgaggtggt 
      tgcgctgcc atggaggtg gcaatgccc atgtcgggt 
      actgccatc gactgctat gaagacatg ttcaaagag 
      ataaccagg aagctctac ggcgaggac ggcttgcct 
      gaattaacc ggaaccgag cgacttccc acaaaccag 
      gatcgtggc ttaactccg atcgattat gacgttgac 
      aatccaatt ttaaagccg gaagaacat ttaactgca 
      tttggctta gctgcgcta atgcaaaat ggtttcccc 
      acaacagga atactcaat tcgacgttt caacaacac 
      aagactact gcaacaacg accgttgag gacaagtgg 
      accgccaat gaagacgct ctccagtgg acgcagtcg 
      aaaattgcc acttataat ccatcacag aaattgttt 
      cgatgcgcg gaatgtgat tgtgtcggg tatctcccg 
      agagttgct gaacattgg ttagggact cactcgaat 
      ttaagagct tttcaatgt ccgcaatgt ccgtacgaa 
      agtgcgtgg gccaggtgt gtacgaatg catttgagt 
      cgccaacac aacatcaac accgacgaa gcttcggac 
      actttggcc aaaaacaac ccaattttg caagaaatc 
      acaaaatac ctccaaagg ctcaaaaat aaattagaa 
      aacaattgt gcgaaacca tcacatata aacaactcg 
      caaatctta gacgagttt caatcttcc tcgacctct 
      accaacaat catctcaac aacaatcaa gtggttgcc 
      acaaactcc gacgcatcc agcagttcc tccaccaaa 
      aggtacacc tgtacctac tgcccctac gctacggac 
      cgcagagac ctatttacg cggcatgag aatattcat 
      cgcgaagag aaaccgttc cagtgttac gtctgtcag 
      aaacaattc aatcgagcg gatcatgtt aaaaagcac 
      ttccttcga atgcacagg gagcaccct tatgatctg 
      aaccggata cgccgacat cctccgaaa aacgcttcg 
      ggaatgtcg tattatcaa aaatacaac gcaaattct 
      acaaatcaa tcaccggat tccatatct tcgctggcg 
      aatattaat tccctttcc attccaaac ggatttaat 
      aacatatcg cgcaatgcc aataatatc agaccaggt 
      aacaccacc gatatgaag aacgggatt acaaacatt 
      aaatctggg aatatttcc aaaggtaat ggaaaaaag 
      aaaggcgag aaacgattc acttgttgt tactgctcc 
      tggtcgggg gttgataac tggtgctta aaacggcac 
      atgaacact catttaaaa ccattcgtg tgcggcctt 
      tgtgattac aaagcagcg agatctgaa agattggca 
      acccacgtg ctgaaagta cacaataaa cgagcttgt 
      ggaaaatgc agttttttg gccgacgat cccgcccaa 
      ttaaccgta caccagcaa gagcaccat cctttggag 
      caacgaaac cgatcagga aacaacatt ttgcgaaat 
      aattccttc aataacagc acggcaacg tcatcatcc 
      tcgtgcagc caaagccag acgagtctg aagccccac 
      gagctgagt gccgctgct gctctgctc cagcacagc 
      aaccgcttg cttcaaaac gacaacaag tggaaaaac 
      cacgctccc aagcaacac ggagctgcg cgtctcttc 
      aattacatg gaagccagc gatgggtcg gagccggag 
      tcggaatca cgcggggcc gaagacatg agtcggttg 
      aacttgaat caccggatt cagtcggat tttcttgag 
      gcgggagat gttggtagc caggccgac gaggaagag 
      ggtgaagag atggagctc cctttgttc gtctgcccc 
      acgtgcggc tgcgagttc gaggacgac atgtcactt 
      gaaacccat caatacacg caccgtcac agcaccaca 
      tctcccgca aaatccgac aactccaag gagaatgtc 
      aaccctaat ttactaaag tatcgatgt tgtttgtgc 
      gattttgcc tttgagaat cagtcttct gttatggcg 
      cacatggtg tctcattcg agcctgagc ggggcgcag 
      gcgaagtcc acgttgacg aagcgctcc cggaaacag 
      agccgcccc aaaaagatg gtcttgccg ttctgggac 
      ttgaacgaa gttgaagtg ttgcggcac ctcaatggg 
      aacttaagc ggcgagctg caacaggcc tctgcctcc 
      atgtccagg aagtacatg gccaggatt gtcgctcgc 
      aaaggactg tattgcgtg ctctgcaag agctccagt 
      gtgaacaga taccacaca aaggcgagc ctagctttg 
      catcactac tggaaacac ttgaacaag cgcttcaag 
      tgcgaacat tgcgaccta catttccgg catcggtat 
      cagtgtgtg ctgcatgcc agtcgcgag catataaat 
      aaaccaccg atccttaag tctcaaaca gtgcctatt 
      aaagaacca attcattcc gcagtacct gcttcccag 
      gacgcccat catttcttc gcaaactct caaactgtt 
      actgtacct gacgcatca tccaatgcg cataaaata 
      gggatgtcg ttttttgac cactcgttc atacacccc 
      accaacgcc atcaacagt catatgccg atcatcata 
      cccactttt ccaccaaat taagccacc ctttgcgtt 
      actattacc tttactatc gacaacttg gctaggtac 
      tataaaaag tgaccttca aaaattttc tttacgtgc 
      tcgtatcgt aagtatttt cggaacaaa cttaaatgc 
      aatcatatt tttcattag tccaaattt tcgtgcaat 
      atctgttac ttggtgatg tttattatt atatttact 
      ttacatatt atgaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY