| General information | |||
| TC013578 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3506275..3510572 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC013578 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05458 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC013578 RA
MEVAMPMSG TAIDCYEDM FKEITRKLY GEDGLPELT GTERLPTNQ DRGLTPIDY DVDNPILKP EEHLTAFGL AALMQNGFP TTGILNSTF QQHKTTATT TVEDKWTAN EDALQWTQS KIATYNPSQ KLFRCAECD CVGYLPRVA EHWLGTHSN LRAFQCPQC PYESAWARC VRMHLSRQH NINTDEASD TLAKNNPIL QEITKYLQR LKNKLENNC AKPSHINNS QILDEFQSS STSTNNHLN NNQVVATNS DASSSSSTK RYTCTYCPY ATDRRDLFT RHENIHREE KPFQCYVCQ KQFNRADHV KKHFLRMHR EHPYDLNRI RRHPPKNAS GMSYYQKYN ANSTNQSPD SISSLANIN SLSIPNGFN NISRNANNI RPGNTTDMK NGITNIKSG NISKGNGKK KGEKRFTCC YCSWSGVDN WCLKRHMNT HLKPFVCGL CDYKAARSE RLATHVLKV HNKRACGKC SFLADDPAQ LTVHQQEHH PLEQRNRSG NNILRNNSF NNSTATSSS SCSQSQTSL KPHELSAAA ALLQHSNRL LQNDNKWKN HAPKQHGAA RLFNYMEAS DGSEPESES RGAEDMSRL NLNHRIQSD FLEAGDVGS QADEEEGEE MELPLFVCP TCGCEFEDD MSLETHQYT HRHSTTSPA KSDNSKENV NPNLLKYRC CLCDFAFEN QSSVMAHMV SHSSLSGAQ AKSTLTKRS RKQSRPKKM VLPFWDLNE VEVLRHLNG NLSGELQQA SASMSRKYM ARIVARKGL YCVLCKSSS VNRYHTKAS LALHHYWKH LNKRFKCEH CDLHFRHRY QCVLHASRE HINKPPILK SQTVPIKEP IHSAVPASQ DAHHFFANS QTVTVPDAS SNAHKIGMS FFDHSFIHP TNAINSHMP IIIPTFPPN
- >TC013578 RA
atggaggtg gcaatgccc atgtcgggt actgccatc gactgctat gaagacatg ttcaaagag ataaccagg aagctctac ggcgaggac ggcttgcct gaattaacc ggaaccgag cgacttccc acaaaccag gatcgtggc ttaactccg atcgattat gacgttgac aatccaatt ttaaagccg gaagaacat ttaactgca tttggctta gctgcgcta atgcaaaat ggtttcccc acaacagga atactcaat tcgacgttt caacaacac aagactact gcaacaacg accgttgag gacaagtgg accgccaat gaagacgct ctccagtgg acgcagtcg aaaattgcc acttataat ccatcacag aaattgttt cgatgcgcg gaatgtgat tgtgtcggg tatctcccg agagttgct gaacattgg ttagggact cactcgaat ttaagagct tttcaatgt ccgcaatgt ccgtacgaa agtgcgtgg gccaggtgt gtacgaatg catttgagt cgccaacac aacatcaac accgacgaa gcttcggac actttggcc aaaaacaac ccaattttg caagaaatc acaaaatac ctccaaagg ctcaaaaat aaattagaa aacaattgt gcgaaacca tcacatata aacaactcg caaatctta gacgagttt caatcttcc tcgacctct accaacaat catctcaac aacaatcaa gtggttgcc acaaactcc gacgcatcc agcagttcc tccaccaaa aggtacacc tgtacctac tgcccctac gctacggac cgcagagac ctatttacg cggcatgag aatattcat cgcgaagag aaaccgttc cagtgttac gtctgtcag aaacaattc aatcgagcg gatcatgtt aaaaagcac ttccttcga atgcacagg gagcaccct tatgatctg aaccggata cgccgacat cctccgaaa aacgcttcg ggaatgtcg tattatcaa aaatacaac gcaaattct acaaatcaa tcaccggat tccatatct tcgctggcg aatattaat tccctttcc attccaaac ggatttaat aacatatcg cgcaatgcc aataatatc agaccaggt aacaccacc gatatgaag aacgggatt acaaacatt aaatctggg aatatttcc aaaggtaat ggaaaaaag aaaggcgag aaacgattc acttgttgt tactgctcc tggtcgggg gttgataac tggtgctta aaacggcac atgaacact catttaaaa ccattcgtg tgcggcctt tgtgattac aaagcagcg agatctgaa agattggca acccacgtg ctgaaagta cacaataaa cgagcttgt ggaaaatgc agttttttg gccgacgat cccgcccaa ttaaccgta caccagcaa gagcaccat cctttggag caacgaaac cgatcagga aacaacatt ttgcgaaat aattccttc aataacagc acggcaacg tcatcatcc tcgtgcagc caaagccag acgagtctg aagccccac gagctgagt gccgctgct gctctgctc cagcacagc aaccgcttg cttcaaaac gacaacaag tggaaaaac cacgctccc aagcaacac ggagctgcg cgtctcttc aattacatg gaagccagc gatgggtcg gagccggag tcggaatca cgcggggcc gaagacatg agtcggttg aacttgaat caccggatt cagtcggat tttcttgag gcgggagat gttggtagc caggccgac gaggaagag ggtgaagag atggagctc cctttgttc gtctgcccc acgtgcggc tgcgagttc gaggacgac atgtcactt gaaacccat caatacacg caccgtcac agcaccaca tctcccgca aaatccgac aactccaag gagaatgtc aaccctaat ttactaaag tatcgatgt tgtttgtgc gattttgcc tttgagaat cagtcttct gttatggcg cacatggtg tctcattcg agcctgagc ggggcgcag gcgaagtcc acgttgacg aagcgctcc cggaaacag agccgcccc aaaaagatg gtcttgccg ttctgggac ttgaacgaa gttgaagtg ttgcggcac ctcaatggg aacttaagc ggcgagctg caacaggcc tctgcctcc atgtccagg aagtacatg gccaggatt gtcgctcgc aaaggactg tattgcgtg ctctgcaag agctccagt gtgaacaga taccacaca aaggcgagc ctagctttg catcactac tggaaacac ttgaacaag cgcttcaag tgcgaacat tgcgaccta catttccgg catcggtat cagtgtgtg ctgcatgcc agtcgcgag catataaat aaaccaccg atccttaag tctcaaaca gtgcctatt aaagaacca attcattcc gcagtacct gcttcccag gacgcccat catttcttc gcaaactct caaactgtt actgtacct gacgcatca tccaatgcg cataaaata gggatgtcg ttttttgac cactcgttc atacacccc accaacgcc atcaacagt catatgccg atcatcata cccactttt ccaccaaat taa
- >TC013578 RA
tcaattgag tgtgtttaa gttgatttt tgtgtgatc atgtttgtt gtccatcgc aggtgtggg cgtaattta aaggtgagt ttgtagaaa ataaatgtt cgtcgagtt ccggcacat cggcgttct tttgagtcc gttcagcct tcggtgagc tcaccctcc cgtaattct ggttgcttg gcaacccct gccagccac cccttaaac ttccccgcg agagacgaa ccccctcgc gcaatagtt tttatatga tagctccga tggctttct tctgtggat ggcgaagct tgcgccctc cgccaccct ccgactcga gcccttaat tctgggatg catcggccg gcttcggag cattattag ggcatgtag gagttgggg cgtcggaat tcgtgcatc ccgtgtccc tcgcgctag agccttgtg tgcactgtg tacagtagg gtgagatct ggaacggcc gcgaaggtg gttgtgggc cgggcgggg tggccgggg tggtcgggg tcgggacgg ggagcgata gtggggtgg catgggggg ggtagggcc gggggcgga gggccccgg gcagcgggt gccgcagtg ttgggtggg tcagatatc gcagaagca gacgtccca tgcgttcgt tacgctctc catagtact agtggttgg tgacatgca gtgatcggt gctttcgtt tcgacggga caagctgac atgctgccc tttgcccac acacaactg gaagacggg cctcagcag tgaggtggt tgcgctgcc atggaggtg gcaatgccc atgtcgggt actgccatc gactgctat gaagacatg ttcaaagag ataaccagg aagctctac ggcgaggac ggcttgcct gaattaacc ggaaccgag cgacttccc acaaaccag gatcgtggc ttaactccg atcgattat gacgttgac aatccaatt ttaaagccg gaagaacat ttaactgca tttggctta gctgcgcta atgcaaaat ggtttcccc acaacagga atactcaat tcgacgttt caacaacac aagactact gcaacaacg accgttgag gacaagtgg accgccaat gaagacgct ctccagtgg acgcagtcg aaaattgcc acttataat ccatcacag aaattgttt cgatgcgcg gaatgtgat tgtgtcggg tatctcccg agagttgct gaacattgg ttagggact cactcgaat ttaagagct tttcaatgt ccgcaatgt ccgtacgaa agtgcgtgg gccaggtgt gtacgaatg catttgagt cgccaacac aacatcaac accgacgaa gcttcggac actttggcc aaaaacaac ccaattttg caagaaatc acaaaatac ctccaaagg ctcaaaaat aaattagaa aacaattgt gcgaaacca tcacatata aacaactcg caaatctta gacgagttt caatcttcc tcgacctct accaacaat catctcaac aacaatcaa gtggttgcc acaaactcc gacgcatcc agcagttcc tccaccaaa aggtacacc tgtacctac tgcccctac gctacggac cgcagagac ctatttacg cggcatgag aatattcat cgcgaagag aaaccgttc cagtgttac gtctgtcag aaacaattc aatcgagcg gatcatgtt aaaaagcac ttccttcga atgcacagg gagcaccct tatgatctg aaccggata cgccgacat cctccgaaa aacgcttcg ggaatgtcg tattatcaa aaatacaac gcaaattct acaaatcaa tcaccggat tccatatct tcgctggcg aatattaat tccctttcc attccaaac ggatttaat aacatatcg cgcaatgcc aataatatc agaccaggt aacaccacc gatatgaag aacgggatt acaaacatt aaatctggg aatatttcc aaaggtaat ggaaaaaag aaaggcgag aaacgattc acttgttgt tactgctcc tggtcgggg gttgataac tggtgctta aaacggcac atgaacact catttaaaa ccattcgtg tgcggcctt tgtgattac aaagcagcg agatctgaa agattggca acccacgtg ctgaaagta cacaataaa cgagcttgt ggaaaatgc agttttttg gccgacgat cccgcccaa ttaaccgta caccagcaa gagcaccat cctttggag caacgaaac cgatcagga aacaacatt ttgcgaaat aattccttc aataacagc acggcaacg tcatcatcc tcgtgcagc caaagccag acgagtctg aagccccac gagctgagt gccgctgct gctctgctc cagcacagc aaccgcttg cttcaaaac gacaacaag tggaaaaac cacgctccc aagcaacac ggagctgcg cgtctcttc aattacatg gaagccagc gatgggtcg gagccggag tcggaatca cgcggggcc gaagacatg agtcggttg aacttgaat caccggatt cagtcggat tttcttgag gcgggagat gttggtagc caggccgac gaggaagag ggtgaagag atggagctc cctttgttc gtctgcccc acgtgcggc tgcgagttc gaggacgac atgtcactt gaaacccat caatacacg caccgtcac agcaccaca tctcccgca aaatccgac aactccaag gagaatgtc aaccctaat ttactaaag tatcgatgt tgtttgtgc gattttgcc tttgagaat cagtcttct gttatggcg cacatggtg tctcattcg agcctgagc ggggcgcag gcgaagtcc acgttgacg aagcgctcc cggaaacag agccgcccc aaaaagatg gtcttgccg ttctgggac ttgaacgaa gttgaagtg ttgcggcac ctcaatggg aacttaagc ggcgagctg caacaggcc tctgcctcc atgtccagg aagtacatg gccaggatt gtcgctcgc aaaggactg tattgcgtg ctctgcaag agctccagt gtgaacaga taccacaca aaggcgagc ctagctttg catcactac tggaaacac ttgaacaag cgcttcaag tgcgaacat tgcgaccta catttccgg catcggtat cagtgtgtg ctgcatgcc agtcgcgag catataaat aaaccaccg atccttaag tctcaaaca gtgcctatt aaagaacca attcattcc gcagtacct gcttcccag gacgcccat catttcttc gcaaactct caaactgtt actgtacct gacgcatca tccaatgcg cataaaata gggatgtcg ttttttgac cactcgttc atacacccc accaacgcc atcaacagt catatgccg atcatcata cccactttt ccaccaaat taagccacc ctttgcgtt actattacc tttactatc gacaacttg gctaggtac tataaaaag tgaccttca aaaattttc tttacgtgc tcgtatcgt aagtatttt cggaacaaa cttaaatgc aatcatatt tttcattag tccaaattt tcgtgcaat atctgttac ttggtgatg tttattatt atatttact ttacatatt atgaaac
