Status
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Gene information
General information
TC031509
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013576,TC013575 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3521310..3527162
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031509
    iBeetle screen iB_05457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031509 RA
      MFGSKLRSW MEAVRPRKK QHRKEKQTK ANLNPNTVT 
ANWQNGTSC GSSQDDLGN TNRKITPEN GQTTQAPAT 
RYSANNLSS PESAYSTGY STDGTSPGA PPDYYINIR 
TGVKYFPAE GERHHHENH LRYVPHHGI GTDLAKPPV 
SSTCLPPPR PVMTQEVDR VVQTRPFQC NTAVTPSRL 
SEVVASPVL TSPRQRNRI RTNPWLPGS APSPGPIRQ 
EVPKITTSV TGTDSPLSR RFRSPSTLR GSSLRNHQS 
LSSSSCSSL SGASVHWDA KKRSGSDED DCTLNEMMG 
KYDESYVYE KETDILSDS DPTDCDTDI DTGQDGGDE 
DEPVDGEFD FIDNGSYLE LDSREDRNT GHCTYYNFE 
VQRKASRRR TTRRSKQSS EKRRRSSSN KKRHKQAER 
ANAQPNGSK SAGATPLSV RRTKHHPMS KLALDKKRS 
NSVSFSRDD PFSTIDKRD KEADQKYRE LIVEAEHIL 
RSMKTNGLS PRRVPGPAN KRVEILRSA ECAKPELFM 
KNRLIEEPG PPFTKIPST NFNRFSPKR NHITNFMIS 
NSPAMMRRE VELPTSPLA KRPESPKLR ANRRSPKYR 
KKASKAKVE CPTSSDSDG EAKVKDAVG FGCPQSEPV 
RRKVYSGRG KTVAFNLNY ENIYNNSPT RFEAQEYKT 
SAENLRQQV LMNTIANLK KSLEDQSAS LKQVYRSSH 
NIQL
    CDS sequence
    • >TC031509 RA
      atgttcgga agcaagctc cgatcatgg atggaagca 
gtgagacct cgcaaaaaa caacaccga aaagagaaa 
caaactaaa gcgaatttg aatcctaac acagttacc 
gccaactgg cagaacgga accagttgc gggtcttcc 
caagatgat ctggggaat actaatcgg aagatcact 
cccgaaaat ggccaaact acccaagca cctgccacg 
cgatattcc gcaaacaat ctatcttct cccgaatcg 
gcctactcg accggatac tccactgat gggacttcc 
ccaggcgct cctccggac tactacatc aacatccgc 
acaggggtg aagtacttc ccagccgag ggggagcgc 
caccaccac gagaaccac ctgagatac gtgccacac 
cacggcatc ggcaccgat ttggctaag cccccagtt 
tcctccact tgtctgcct ccacccaga cccgtcatg 
acgcaagag gtggaccga gtggtccag acacgtccg 
ttccagtgc aacaccgct gtcaccccg tccagacta 
agcgaagtt gtcgccagt ccggtcctc acatcccca 
agacagcgc aaccggatc aggacgaac ccctggctt 
ccgggaagc gccccaagc ccggggccc atcaggcag 
gaggtgccc aaaatcaca acttcagtc actgggact 
gacagcccg ctgtcgagg aggttccga agcccgtcc 
acgctccgc gggtcgagt ttgcgaaac caccagtcc 
ctgtcctcc tcgtcttgt tcctcccta agcggggcg 
agtgtccac tgggacgcg aagaagcgg tccgggagc 
gacgaagac gactgtacc ctgaacgaa atgatgggc 
aaatacgac gagagttac gtttacgag aaagaaacc 
gacattctt agcgatagc gacccgact gattgtgat 
actgacatt gacacgggc caagacggc ggagacgaa 
gacgagccc gtcgacggc gagttcgac ttcatagac 
aacggctcc tacctggag ctcgactcc cgcgaggac 
cggaacacc ggccattgc acgtattac aacttcgaa 
gtccagcgg aaagcctcc cggaggcgc acgactcgc 
cgcagcaag cagagcagc gagaagcgg cgccggtca 
agttccaac aagaaacgg cacaagcaa gccgagcgc 
gcgaacgcg cagcccaac ggctcgaaa agcgcgggt 
gccactccc ttgtccgtc cgtcgaacc aagcaccat 
ccaatgtcg aagctggcc ctcgacaag aagcgctcc 
aactccgtc agcttctcc cgcgacgat cccttctca 
accattgac aagcgggac aaagaggcc gaccagaag 
taccgcgag ttgatagtc gaggccgag cacatcctg 
cgcagcatg aagaccaac ggcctttcg ccgcggcgg 
gtcccaggc cccgccaac aagcgcgtc gagattttg 
cggagcgcg gaatgtgcc aaaccggag cttttcatg 
aaaaataga ctgatcgaa gagccaggt cccccattt 
accaagata ccgtccact aattttaat agatttagt 
cctaaacga aaccacatt actaacttc atgatcagc 
aattcgccg gcgatgatg cgccgcgag gtggagttg 
cccacttcg ccgctggcg aagcggccg gagtcgccc 
aagttgcgg gcgaaccgg cgcagtccg aagtaccgg 
aaaaaggcg agcaaggcg aaagtggag tgccccacg 
agcagcgac tccgacggg gaggcgaaa gttaaggac 
gccgtcggt ttcgggtgt ccgcagagc gagccggtg 
cgccggaag gtgtactca gggcgggga aaaaccgtg 
gcgttcaat ctcaattac gagaatatt tacaacaat 
tcgccgacg aggttcgag gcgcaggag tacaagact 
agcgcggag aatttaaga cagcaggtt ttaatgaac 
accatcgcc aatttgaag aagagtctc gaagatcag 
tccgcttcg ctgaagcag gtgtaccga agttcccat 
aatatacaa ctctaa
    mRNA sequence
    • >TC031509 RA
      aagaaaatt gttggtggt tgtacgcac acgcctgtg 
aaacatggc tcaaaatcc atattattt gttagattt 
tagttactc attaactaa tcggtgctc taattattg 
ataattgac tgtggttga aatcgtgac taaagatgt 
tcggaagca agctccgat catggatgg aagcagtga 
gacctcgca aaaaacaac accgaaaag agaaacaaa 
ctaaagcga atttgaatc ctaacacag ttaccgcca 
actggcaga acggaacca gttgcgggt cttcccaag 
atgatctgg ggaatacta atcggaaga tcactcccg 
aaaatggcc aaactaccc aagcacctg ccacgcgat 
attccgcaa acaatctat cttctcccg aatcggcct 
actcgaccg gatactcca ctgatggga cttccccag 
gcgctcctc cggactact acatcaaca tccgcacag 
gggtgaagt acttcccag ccgaggggg agcgccacc 
accacgaga accacctga gatacgtgc cacaccacg 
gcatcggca ccgatttgg ctaagcccc cagtttcct 
ccacttgtc tgcctccac ccagacccg tcatgacgc 
aagaggtgg accgagtgg tccagacac gtccgttcc 
agtgcaaca ccgctgtca ccccgtcca gactaagcg 
aagttgtcg ccagtccgg tcctcacat ccccaagac 
agcgcaacc ggatcagga cgaacccct ggcttccgg 
gaagcgccc caagcccgg ggcccatca ggcaggagg 
tgcccaaaa tcacaactt cagtcactg ggactgaca 
gcccgctgt cgaggaggt tccgaagcc cgtccacgc 
tccgcgggt cgagtttgc gaaaccacc agtccctgt 
cctcctcgt cttgttcct ccctaagcg gggcgagtg 
tccactggg acgcgaaga agcggtccg ggagcgacg 
aagacgact gtaccctga acgaaatga tgggcaaat 
acgacgaga gttacgttt acgagaaag aaaccgaca 
ttcttagcg atagcgacc cgactgatt gtgatactg 
acattgaca cgggccaag acggcggag acgaagacg 
agcccgtcg acggcgagt tcgacttca tagacaacg 
gctcctacc tggagctcg actcccgcg aggaccgga 
acaccggcc attgcacgt attacaact tcgaagtcc 
agcggaaag cctcccgga ggcgcacga ctcgccgca 
gcaagcaga gcagcgaga agcggcgcc ggtcaagtt 
ccaacaaga aacggcaca agcaagccg agcgcgcga 
acgcgcagc ccaacggct cgaaaagcg cgggtgcca 
ctcccttgt ccgtccgtc gaaccaagc accatccaa 
tgtcgaagc tggccctcg acaagaagc gctccaact 
ccgtcagct tctcccgcg acgatccct tctcaacca 
ttgacaagc gggacaaag aggccgacc agaagtacc 
gcgagttga tagtcgagg ccgagcaca tcctgcgca 
gcatgaaga ccaacggcc tttcgccgc ggcgggtcc 
caggccccg ccaacaagc gcgtcgaga ttttgcgga 
gcgcggaat gtgccaaac cggagcttt tcatgaaaa 
atagactga tcgaagagc caggtcccc catttacca 
agataccgt ccactaatt ttaatagat ttagtccta 
aacgaaacc acattacta acttcatga tcagcaatt 
cgccggcga tgatgcgcc gcgaggtgg agttgccca 
cttcgccgc tggcgaagc ggccggagt cgcccaagt 
tgcgggcga accggcgca gtccgaagt accggaaaa 
aggcgagca aggcgaaag tggagtgcc ccacgagca 
gcgactccg acggggagg cgaaagtta aggacgccg 
tcggtttcg ggtgtccgc agagcgagc cggtgcgcc 
ggaaggtgt actcagggc ggggaaaaa ccgtggcgt 
tcaatctca attacgaga atatttaca acaattcgc 
cgacgaggt tcgaggcgc aggagtaca agactagcg 
cggagaatt taagacagc aggttttaa tgaacacca 
tcgccaatt tgaagaaga gtctcgaag atcagtccg 
cttcgctga agcaggtgt accgaagtt cccataata 
tacaactct aagttacag cgtatacaa atatattta 
taattttat accgtggat gttgaagac aaacaccag 
tagagtagc ggttcggtt gattcctgg cgtttatta 
acccaaatg tagccgttt aaatattta agcagacgt 
agaattgtt ttcggttcg atgtagcga caatagtag 
aaactagtg acgtagcgg tataattgt agggtaact 
tgtcggccc acttagtta agctaacac aaaaacttt 
cagaataat aaaaattga tttggttgg tagtcgttt 
taagtgggc gacaatcac ttatgtaac atgatagta 
aaatgtagt acaatttag tataatttg aatgtggaa 
tctggatcg gactaataa tctgttaat gacgtcttt 
gattgtgtt gaagcaaat acggccttg ttacttttg 
actttaatt ttaggtata gttgaatta tgacagtaa 
gtgttgatt tgttatttc ctgcaaaaa ggaaatgtt 
attttgaga gaaataaca ctaaaatcg agtttaact 
gactgaagg gtactccaa ataaaagtg cggagattt 
cacgcattc cctgttaaa actgtgcta tatttttaa 
taggtatat gtattttta taggcacta atacaaata 
cgtaaccgc ctatatgta tactttaat cacagtcgt 
tattcgaca tgtgcctgt tttaacttt cgatttcac 
ttcacagct aagctataa ctgtattca tgttgtgat 
tcctgaatt gttaaattt ttcaaatgt tcatctgta 
aataaaaac aataacttt cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY