| General information | |||
| TC031509
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3521310..3527162 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031509 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05457 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
- >TC031509 RA
MFGSKLRSW MEAVRPRKK QHRKEKQTK ANLNPNTVT ANWQNGTSC GSSQDDLGN TNRKITPEN GQTTQAPAT RYSANNLSS PESAYSTGY STDGTSPGA PPDYYINIR TGVKYFPAE GERHHHENH LRYVPHHGI GTDLAKPPV SSTCLPPPR PVMTQEVDR VVQTRPFQC NTAVTPSRL SEVVASPVL TSPRQRNRI RTNPWLPGS APSPGPIRQ EVPKITTSV TGTDSPLSR RFRSPSTLR GSSLRNHQS LSSSSCSSL SGASVHWDA KKRSGSDED DCTLNEMMG KYDESYVYE KETDILSDS DPTDCDTDI DTGQDGGDE DEPVDGEFD FIDNGSYLE LDSREDRNT GHCTYYNFE VQRKASRRR TTRRSKQSS EKRRRSSSN KKRHKQAER ANAQPNGSK SAGATPLSV RRTKHHPMS KLALDKKRS NSVSFSRDD PFSTIDKRD KEADQKYRE LIVEAEHIL RSMKTNGLS PRRVPGPAN KRVEILRSA ECAKPELFM KNRLIEEPG PPFTKIPST NFNRFSPKR NHITNFMIS NSPAMMRRE VELPTSPLA KRPESPKLR ANRRSPKYR KKASKAKVE CPTSSDSDG EAKVKDAVG FGCPQSEPV RRKVYSGRG KTVAFNLNY ENIYNNSPT RFEAQEYKT SAENLRQQV LMNTIANLK KSLEDQSAS LKQVYRSSH NIQL
- >TC031509 RA
atgttcgga agcaagctc cgatcatgg atggaagca gtgagacct cgcaaaaaa caacaccga aaagagaaa caaactaaa gcgaatttg aatcctaac acagttacc gccaactgg cagaacgga accagttgc gggtcttcc caagatgat ctggggaat actaatcgg aagatcact cccgaaaat ggccaaact acccaagca cctgccacg cgatattcc gcaaacaat ctatcttct cccgaatcg gcctactcg accggatac tccactgat gggacttcc ccaggcgct cctccggac tactacatc aacatccgc acaggggtg aagtacttc ccagccgag ggggagcgc caccaccac gagaaccac ctgagatac gtgccacac cacggcatc ggcaccgat ttggctaag cccccagtt tcctccact tgtctgcct ccacccaga cccgtcatg acgcaagag gtggaccga gtggtccag acacgtccg ttccagtgc aacaccgct gtcaccccg tccagacta agcgaagtt gtcgccagt ccggtcctc acatcccca agacagcgc aaccggatc aggacgaac ccctggctt ccgggaagc gccccaagc ccggggccc atcaggcag gaggtgccc aaaatcaca acttcagtc actgggact gacagcccg ctgtcgagg aggttccga agcccgtcc acgctccgc gggtcgagt ttgcgaaac caccagtcc ctgtcctcc tcgtcttgt tcctcccta agcggggcg agtgtccac tgggacgcg aagaagcgg tccgggagc gacgaagac gactgtacc ctgaacgaa atgatgggc aaatacgac gagagttac gtttacgag aaagaaacc gacattctt agcgatagc gacccgact gattgtgat actgacatt gacacgggc caagacggc ggagacgaa gacgagccc gtcgacggc gagttcgac ttcatagac aacggctcc tacctggag ctcgactcc cgcgaggac cggaacacc ggccattgc acgtattac aacttcgaa gtccagcgg aaagcctcc cggaggcgc acgactcgc cgcagcaag cagagcagc gagaagcgg cgccggtca agttccaac aagaaacgg cacaagcaa gccgagcgc gcgaacgcg cagcccaac ggctcgaaa agcgcgggt gccactccc ttgtccgtc cgtcgaacc aagcaccat ccaatgtcg aagctggcc ctcgacaag aagcgctcc aactccgtc agcttctcc cgcgacgat cccttctca accattgac aagcgggac aaagaggcc gaccagaag taccgcgag ttgatagtc gaggccgag cacatcctg cgcagcatg aagaccaac ggcctttcg ccgcggcgg gtcccaggc cccgccaac aagcgcgtc gagattttg cggagcgcg gaatgtgcc aaaccggag cttttcatg aaaaataga ctgatcgaa gagccaggt cccccattt accaagata ccgtccact aattttaat agatttagt cctaaacga aaccacatt actaacttc atgatcagc aattcgccg gcgatgatg cgccgcgag gtggagttg cccacttcg ccgctggcg aagcggccg gagtcgccc aagttgcgg gcgaaccgg cgcagtccg aagtaccgg aaaaaggcg agcaaggcg aaagtggag tgccccacg agcagcgac tccgacggg gaggcgaaa gttaaggac gccgtcggt ttcgggtgt ccgcagagc gagccggtg cgccggaag gtgtactca gggcgggga aaaaccgtg gcgttcaat ctcaattac gagaatatt tacaacaat tcgccgacg aggttcgag gcgcaggag tacaagact agcgcggag aatttaaga cagcaggtt ttaatgaac accatcgcc aatttgaag aagagtctc gaagatcag tccgcttcg ctgaagcag gtgtaccga agttcccat aatatacaa ctctaa
- >TC031509 RA
aagaaaatt gttggtggt tgtacgcac acgcctgtg aaacatggc tcaaaatcc atattattt gttagattt tagttactc attaactaa tcggtgctc taattattg ataattgac tgtggttga aatcgtgac taaagatgt tcggaagca agctccgat catggatgg aagcagtga gacctcgca aaaaacaac accgaaaag agaaacaaa ctaaagcga atttgaatc ctaacacag ttaccgcca actggcaga acggaacca gttgcgggt cttcccaag atgatctgg ggaatacta atcggaaga tcactcccg aaaatggcc aaactaccc aagcacctg ccacgcgat attccgcaa acaatctat cttctcccg aatcggcct actcgaccg gatactcca ctgatggga cttccccag gcgctcctc cggactact acatcaaca tccgcacag gggtgaagt acttcccag ccgaggggg agcgccacc accacgaga accacctga gatacgtgc cacaccacg gcatcggca ccgatttgg ctaagcccc cagtttcct ccacttgtc tgcctccac ccagacccg tcatgacgc aagaggtgg accgagtgg tccagacac gtccgttcc agtgcaaca ccgctgtca ccccgtcca gactaagcg aagttgtcg ccagtccgg tcctcacat ccccaagac agcgcaacc ggatcagga cgaacccct ggcttccgg gaagcgccc caagcccgg ggcccatca ggcaggagg tgcccaaaa tcacaactt cagtcactg ggactgaca gcccgctgt cgaggaggt tccgaagcc cgtccacgc tccgcgggt cgagtttgc gaaaccacc agtccctgt cctcctcgt cttgttcct ccctaagcg gggcgagtg tccactggg acgcgaaga agcggtccg ggagcgacg aagacgact gtaccctga acgaaatga tgggcaaat acgacgaga gttacgttt acgagaaag aaaccgaca ttcttagcg atagcgacc cgactgatt gtgatactg acattgaca cgggccaag acggcggag acgaagacg agcccgtcg acggcgagt tcgacttca tagacaacg gctcctacc tggagctcg actcccgcg aggaccgga acaccggcc attgcacgt attacaact tcgaagtcc agcggaaag cctcccgga ggcgcacga ctcgccgca gcaagcaga gcagcgaga agcggcgcc ggtcaagtt ccaacaaga aacggcaca agcaagccg agcgcgcga acgcgcagc ccaacggct cgaaaagcg cgggtgcca ctcccttgt ccgtccgtc gaaccaagc accatccaa tgtcgaagc tggccctcg acaagaagc gctccaact ccgtcagct tctcccgcg acgatccct tctcaacca ttgacaagc gggacaaag aggccgacc agaagtacc gcgagttga tagtcgagg ccgagcaca tcctgcgca gcatgaaga ccaacggcc tttcgccgc ggcgggtcc caggccccg ccaacaagc gcgtcgaga ttttgcgga gcgcggaat gtgccaaac cggagcttt tcatgaaaa atagactga tcgaagagc caggtcccc catttacca agataccgt ccactaatt ttaatagat ttagtccta aacgaaacc acattacta acttcatga tcagcaatt cgccggcga tgatgcgcc gcgaggtgg agttgccca cttcgccgc tggcgaagc ggccggagt cgcccaagt tgcgggcga accggcgca gtccgaagt accggaaaa aggcgagca aggcgaaag tggagtgcc ccacgagca gcgactccg acggggagg cgaaagtta aggacgccg tcggtttcg ggtgtccgc agagcgagc cggtgcgcc ggaaggtgt actcagggc ggggaaaaa ccgtggcgt tcaatctca attacgaga atatttaca acaattcgc cgacgaggt tcgaggcgc aggagtaca agactagcg cggagaatt taagacagc aggttttaa tgaacacca tcgccaatt tgaagaaga gtctcgaag atcagtccg cttcgctga agcaggtgt accgaagtt cccataata tacaactct aagttacag cgtatacaa atatattta taattttat accgtggat gttgaagac aaacaccag tagagtagc ggttcggtt gattcctgg cgtttatta acccaaatg tagccgttt aaatattta agcagacgt agaattgtt ttcggttcg atgtagcga caatagtag aaactagtg acgtagcgg tataattgt agggtaact tgtcggccc acttagtta agctaacac aaaaacttt cagaataat aaaaattga tttggttgg tagtcgttt taagtgggc gacaatcac ttatgtaac atgatagta aaatgtagt acaatttag tataatttg aatgtggaa tctggatcg gactaataa tctgttaat gacgtcttt gattgtgtt gaagcaaat acggccttg ttacttttg actttaatt ttaggtata gttgaatta tgacagtaa gtgttgatt tgttatttc ctgcaaaaa ggaaatgtt attttgaga gaaataaca ctaaaatcg agtttaact gactgaagg gtactccaa ataaaagtg cggagattt cacgcattc cctgttaaa actgtgcta tatttttaa taggtatat gtattttta taggcacta atacaaata cgtaaccgc ctatatgta tactttaat cacagtcgt tattcgaca tgtgcctgt tttaacttt cgatttcac ttcacagct aagctataa ctgtattca tgttgtgat tcctgaatt gttaaattt ttcaaatgt tcatctgta aataaaaac aataacttt cc
