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Gene information
General information
TC031511
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013568
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3568778..3575742
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031511
    iBeetle screen iB_05455

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031511 RA
      MKCISVLLF ITIFGGTWR LGRPTCVLE SDFGFTLNC 
      AFKKSGLFR VRNLGGVKA HIGLGFSVG DELGFKESL 
      SNVENNRRR SAQASRLQA ELIQAQQKM KAENQIRAR 
      SPADLPKKS ALPTIPPFK PIPPAPERP DAPKSGIPT 
      PAVMGIAVP AYNPMPSPI SRLSAGPAL PEPSKPKVM 
      AVVVPMNKV QPSDKLSST NFALRTLPS PNNELPPPS 
      TSSSVQALL AANNITEDM LQNALLQRQ QMLAKTRPT 
      TTTPKTSVV GNKYANSVG KVMNAPKEY YPVGYDKNF 
      DDNFASRVE LPETSFYCG DQKHFPGLY ADEDLGCMV 
      FHVCALTDD GLIMKSFLC PESTLFDQT ILKCNWWFY 
      VDCKNSKKL YDSNIPISK SYQLMKALT FFSGYKKDS 
      ATTPKSKDK D
    CDS sequence
    • >TC031511 RA
      atgaagtgt atcagtgta ttgttgttt ataactata 
      tttggcggc acatggcgg ctaggccga ccgacctgc 
      gttttggaa tcagatttc ggatttacc ctcaactgt 
      gcttttaag aaatctggc ctgtttcga gttagaaat 
      cttggtggt gtcaaggct cacataggc ctgggtttt 
      agtgtcgga gatgagcta gggtttaaa gaatctctt 
      tctaatgtc gagaacaat agaagacga tcggcccaa 
      gcgtctcgt ttgcaggcc gagctgatt caagcccag 
      caaaaaatg aaggctgag aatcagatt cgagcgcgt 
      tcgccagcc gatcttccg aaaaaatcg gctttgccc 
      acgatcccg ccgttcaag cccataccc ccggccccg 
      gagcgcccg gacgccccc aagtcagga atcccgacc 
      ccggctgtg atgggcatt gcagtgccc gcgtacaac 
      cccatgcct tcccccatt tcgcggctc tcagcggga 
      cccgccctg cccgagccc tccaagccc aaagtgatg 
      gcggtcgtc gtcccaatg aacaaagtg caaccaagt 
      gataaatta agtagtaca aattttgca ttaaggacg 
      ctgccctcg cccaacaac gagctgccg cccccatct 
      acctcaagc tccgtgcag gcgctcctg gcggcgaac 
      aacatcacc gaagacatg ctgcagaac gccctgctg 
      cagaggcag caaatgctg gccaagaca agaccgacc 
      acaaccact ccaaaaaca agtgtagtc ggcaataag 
      tatgccaac agcgtcgga aaagtcatg aatgcgccc 
      aaggaatat tatccggtg gggtatgat aagaacttt 
      gacgacaat ttcgcctca agggtcgaa ctgccggaa 
      acctccttt tactgcggg gaccagaaa cacttcccg 
      ggtctctac gccgatgag gatctaggc tgcatggtg 
      tttcacgtt tgcgcttta accgacgat ggtctgatt 
      atgaagagc ttcctttgc ccggaatcc acattgttc 
      gaccaaaca atactcaag tgcaattgg tggttttac 
      gtcgattgc aaaaattcg aaaaaactc tacgactcg 
      aatataccc atatcgaag agttatcag ctaatgaaa 
      gcgctaaca ttcttctcc gggtacaaa aaagacagc 
      gcgacgaca cccaaatcg aaagataaa gactga
    mRNA sequence
    • >TC031511 RA
      aaagaaggg ggtaaccat tcataccgt tactttgca 
      gctgcactg ggtcaactt aacagtctt tccatcgac 
      atctgaaga gcggagtcg aggtgtgcg agttggtgt 
      ttagccgtc aaggtgctt cagtgcgtg tagtgaatg 
      ttgttattt tactttgtg cggcaatga agtgtatca 
      gtgtattgt tgtttataa ctatatttg gcggcacat 
      ggcggctag gccgaccga cctgcgttt tggaatcag 
      atttcggat ttaccctca actgtgctt ttaagaaat 
      ctggcctgt ttcgagtta gaaatcttg gtggtgtca 
      aggctcaca taggcctgg gttttagtg tcggagatg 
      agctagggt ttaaagaat ctctttcta atgtcgaga 
      acaatagaa gacgatcgg cccaagcgt ctcgtttgc 
      aggccgagc tgattcaag cccagcaaa aaatgaagg 
      ctgagaatc agattcgag cgcgttcgc cagccgatc 
      ttccgaaaa aatcggctt tgcccacga tcccgccgt 
      tcaagccca tacccccgg ccccggagc gcccggacg 
      cccccaagt caggaatcc cgaccccgg ctgtgatgg 
      gcattgcag tgcccgcgt acaacccca tgccttccc 
      ccatttcgc ggctctcag cgggacccg ccctgcccg 
      agccctcca agcccaaag tgatggcgg tcgtcgtcc 
      caatgaaca aagtgcaac caagtgata aattaagta 
      gtacaaatt ttgcattaa ggacgctgc cctcgccca 
      acaacgagc tgccgcccc catctacct caagctccg 
      tgcaggcgc tcctggcgg cgaacaaca tcaccgaag 
      acatgctgc agaacgccc tgctgcaga ggcagcaaa 
      tgctggcca agacaagac cgaccacaa ccactccaa 
      aaacaagtg tagtcggca ataagtatg ccaacagcg 
      tcggaaaag tcatgaatg cgcccaagg aatattatc 
      cggtggggt atgataaga actttgacg acaatttcg 
      cctcaaggg tcgaactgc cggaaacct ccttttact 
      gcggggacc agaaacact tcccgggtc tctacgccg 
      atgaggatc taggctgca tggtgtttc acgtttgcg 
      ctttaaccg acgatggtc tgattatga agagcttcc 
      tttgcccgg aatccacat tgttcgacc aaacaatac 
      tcaagtgca attggtggt tttacgtcg attgcaaaa 
      attcgaaaa aactctacg actcgaata tacccatat 
      cgaagagtt atcagctaa tgaaagcgc taacattct 
      tctccgggt acaaaaaag acagcgcga cgacaccca 
      aatcgaaag ataaagact gatattttt aaaatttat 
      ttttagaat aatcaattt ttgttaagt gttagcaag 
      gagtcccta tagcaaata gtttgtttc ccaatcgga 
      caccaattt attcacgtt acagtctaa ctggtttcc 
      ccactttca cattaatga aagcatttg atttaaagc 
      agatcaagc gaatcatca tattcatcc attgcttag 
      aaaactaac agtttgcag ttgaacaga aaaactttc 
      attgccttg aaaaatgaa aattgttaa ataacacgt 
      gattttaag tattaatag actgtgagt tgccttctc 
      cttgtttaa cctaagctc aatgtaaaa atctaagtc 
      ttattattt attgttaca tagtggtta ttagatact 
      tacttcgct aattaagat gtttatcgg atctaagaa 
      taattagca tttttatag cattatata ctcgtctga 
      ttgttttat ttgtaatgt ttggaaatg tccaagtgg 
      ctctttacc cgtctagtg aatttttcg gccccctta 
      cgcaaaaaa acgatcagt agcttaaat tttatttca 
      tacatctag tcaaatatt tcatgtcag gcctaattt 
      cagtaaata ttcttatct gtctggact gctaaatag 
      ccactgcca ttgtgtatt cacgggggt acatgacaa 
      ttttttatt tagatttat tataacgat atgaaatgg 
      ttaggttcc tccaagaat gttttatcg tattgttat 
      taccaaata tttatattt ttaattctc atgtttggg 
      gagtttctg tgaatgttt gttaatact ttttgtata 
      acggcattt tattgttgg taaataaat tatttatta 
      catt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY