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Gene information
General information
TC013559 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7952110..7953997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013559
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05452

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva anterior decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013559 RA
      MLTFFLLTA TAVLSDAAL DINTVPPYV KQCYEGDPK 
LIECFESAV HHLRPYLAT GIPEIELPS VEPFLMDEL 
SLSLTTGPN GYKVSLKEI DIFGASNFT LSKLKLSEN 
GKPFEARIN IPLLRINSR YTSSGVLII LPASGNGTF 
NAQLEDINA VVKGMVSVH KKENLTFLH VDSLNLDLT 
VRNLHMSVK NIYKNNRIL TEAINLFLR ENGQEVFKV 
MKPQLRKKL STLFMSITN KLLTHLPVE VFYVPVARQ 
AKKPPQ
    CDS sequence
    • >TC013559 RA
      atgttgact tttttcctg ctgaccgca actgctgtg 
ctttcggac gccgcccta gacatcaat accgtgcct 
ccttatgta aagcagtgc tatgagggc gatcccaag 
ctcattgaa tgcttcgaa agtgcagtc catcactta 
cggccctat ttggcgacc ggcattcca gaaatagag 
ctgccttcg gtcgaacct ttcctcatg gacgagctg 
tccttgtct ctaacgaca ggaccaaac ggttacaaa 
gtctcgttg aaagaaatt gatattttt ggggccagc 
aacttcacc ctatccaag ctcaaattg agcgaaaac 
gggaagcct tttgaggcc aggattaac attccgcta 
ttgaggatc aattcgaga tacaccagc agtggcgta 
ctcattatt ttgcccgcg agtggaaac ggcactttc 
aacgcccag ttggaggac attaatgcc gtggtcaag 
gggatggtc agtgtgcac aagaaggag aatttaacg 
ttccttcat gtggactcg ttgaattta gacctaaca 
gtgagaaat ttgcacatg tctgttaaa aacatctac 
aagaacaac cgaatttta accgaagca ataaatttg 
tttttgaga gagaatgga caggaagtt tttaaagtg 
atgaagcct caattgcgg aagaaactg tccacactt 
tttatgagt attactaat aagcttttg acacatcta 
cctgttgaa gtattttac gtgccggtg gctagacag 
gcaaagaaa ccaccgcaa taa
    mRNA sequence
    • >TC013559 RA
      acctgtgaa aaaaaattg cattactca attcactcc 
ccacgacgt gaggttgca aaataagaa actctcgat 
tgctgatat ttaaacacc acattttat atcaaccgt 
tgcttaatt ataagaaac catgaaagt tgaacagta 
tgttccaaa ttaatagta aaaactaaa catattcat 
tactggaga accacgttt gaaacgtta agtgaattc 
gccgcttat tccattacc tacaagaga aagtgaaaa 
caaactttt gttaagttc atttatcac aagtattca 
tcaaatcgg caccgactt tgctgctga cagtgtcgt 
gtgattctg caaaatgtt gactttttt cctgctgac 
cgcaactgc tgtgctttc ggacgccgc cctagacat 
caataccgt gcctcctta tgtaaagca gtgctatga 
gggcgatcc caagctcat tgaatgctt cgaaagtgc 
agtccatca cttacggcc ctatttggc gaccggcat 
tccagaaat agagctgcc ttcggtcga acctttcct 
catggacga gctgtcctt gtctctaac gacaggacc 
aaacggtta caaagtctc gttgaaaga aattgatat 
ttttggggc cagcaactt caccctatc caagctcaa 
attgagcga aaacgggaa gccttttga ggccaggat 
taacattcc gctattgag gatcaattc gagatacac 
cagcagtgg cgtactcat tattttgcc cgcgagtgg 
aaacggcac tttcaacgc ccagttgga ggacattaa 
tgccgtggt caaggggat ggtcagtgt gcacaagaa 
ggagaattt aacgttcct tcatgtgga ctcgttgaa 
tttagacct aacagtgag aaatttgca catgtctgt 
taaaaacat ctacaagaa caaccgaat tttaaccga 
agcaataaa tttgttttt gagagagaa tggacagga 
agtttttaa agtgatgaa gcctcaatt gcggaagaa 
actgtccac actttttat gagtattac taataagct 
tttgacaca tctacctgt tgaagtatt ttacgtgcc 
ggtggctag acaggcaaa gaaaccacc gcaataagt 
gtctaatca ttacgtatt tcattgctt tattttgtg 
ttattttag tgatataga tcagtgaat tagttagtc 
tacgtaatt ttgtactat ttattttat ttttgtgtg 
tagaggttc ttttttact ttgttataa aattgttat 
aaaatattt aaaaataat aaattcttt ttttaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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