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Gene information
General information
TC013553 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8101361..8105747
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013553
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05451

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     mandible size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    maxilla not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number # of remaining segments decreased -
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013553 RA
      MIDKDMNSA CMRGGSVRT LNNYQQVME PRSPHTAWQ 
FGVSQIVKR EPMDEDKND SGVTSGSDF HSSSPSSDT 
SQDLQHSYQ SPQTQPARF YSTPIVPHF AYNHNPLTP 
PNSEPLVSP KSEKEEKDM ETTLTPCAS PNRKPDDNQ 
DHLRRLEMS LEKSGLFSS KTSEHSVDE LSGKSDNDA 
EEYDEQSLR VPKVNSHGK IKTFKCKQC DFVAITKLE 
QWNHSKVHI REDKRLTCP KCPFITEYK HHLEYHLRN 
HAGSKPFQC NKCDYTCVN KSMLNSHMK SHSNVYRYS 
CRDCSYATK YCHSLKIHL RRYGHTPNV VLDEEGNPC 
PDIIIDVHG TRRGPKIKT QPKAEEAKP ETLPFLNLQ 
QQLPFPGYP FFGGFPNAQ LLQQLIRER QLAAGGSQE 
ESRVLDLSK PGCSYTGEQ KSRRKGPAF KVDPTQVES 
EEEDEETST TVFSNVEVV QEEAKKEES DSNNNNNKE 
EGNSCQYCN IAFGDAVLY TIHMGYHGF HNPFTCNMC 
GVECSDKVS FFLHIARVS HS
    • >TC013553 RB
      MAICDLAWS ETYFSFMFE RGRSVVSLK FNHCFLSSV 
VFLKNCSSA CEMIDKDMN SACMRGGSV RTLNNYQQV 
MEPRSPHTA WQFGVSQIV KREPMDEDK NDSGVTSGS 
DFHSSSPSS DTSQDLQHS YQSPQTQPA RFYSTPIVP 
HFAYNHNPL TPPNSEPLV SPKSEKEEK DMETTLTPC 
ASPNRKPDD NQDHLRRLE MSLEKSGLF SSKTSEHSV 
DELSGKSDN DAEEYDEQS LRVPKVNSH GKIKTFKCK 
QCDFVAITK LEQWNHSKV HIREDKRLT CPKCPFITE 
YKHHLEYHL RNHAGSKPF QCNKCDYTC VNKSMLNSH 
MKSHSNVYR YSCRDCSYA TKYCHSLKI HLRRYGHTP 
NVVLDEEGN PCPDIIIDV HGTRRGPKI KTQPKAEEA 
KPETLPFLN LQQQLPFPG YPFFGGFPN AQLLQQLIR 
ERQLAAGGS QEESRVLDL SKPGCSYTG EQKSRRKGP 
AFKVDPTQV ESEEEDEET STTVFSNVE VVQEEAKKE 
ESDSNNNNN KEEGNSCQY CNIAFGDAV LYTIHMGYH 
GFHNPFTCN MCGVECSDK VSFFLHIAR VSHS
    CDS sequence
    • >TC013553 RA
      atgatagat aaagacatg aattcggct tgcatgagg 
ggtggctca gtccggact ttaaacaat taccaacaa 
gtgatggaa ccgcggtcc ccccacacc gcctggcaa 
ttcggcgtt tcccagatc gtgaaacgc gaaccaatg 
gacgaagat aagaacgat tccggggtt acctccgga 
agcgacttc cattcatcc tcccccagc agtgatacg 
agccaggat ttacagcac tcttatcaa tcgccgcaa 
acgcaaccg gcccgcttc tactccact cccatcgtg 
ccccacttc gcctacaac cacaacccc ctaacaccc 
cccaactcc gagccactg gtctccccc aagagcgag 
aaggaggag aaggacatg gagacgacc ctcacgcct 
tgcgcgtcc ccgaaccgc aagccggac gacaatcag 
gaccacctc cgccgcctg gagatgtcc ctggagaag 
agcggcctg ttctcgtcc aaaacgagc gaacactcc 
gtggatgag ttgtcgggg aagagcgac aacgacgcc 
gaggagtac gacgagcag agcttgcgc gtcccgaaa 
gtcaactcg cacgggaaa atcaaaact ttcaaatgc 
aaacagtgc gattttgtc gcaatcacc aaactcgaa 
cagtggaac cacagcaag gtgcacatc cgggaggac 
aagcggctg acctgcccc aagtgcccg ttcatcacc 
gagtacaag caccacctg gagtaccac ctccgcaac 
cacgccggg tccaaaccg ttccaatgc aacaaatgc 
gactacact tgtgtgaac aagtccatg ctaaactca 
cacatgaag tcgcactcg aacgtctac cgttatagt 
tgccgggat tgcagctac gctaccaaa tattgccac 
tctttgaag atccacctg aggcggtat ggacacacc 
ccgaacgtg gttttggac gaggaagga aacccgtgt 
ccggacatc atcatcgac gtgcatggg acgcgtcgg 
ggtcccaaa attaagaca caacctaaa gccgaagag 
gcgaaaccg gaaacgctc ccgttcctt aacctgcaa 
caacaacta cctttccct ggttatccc ttctttggg 
gggttcccc aacgcccag ttgttgcaa caactgatc 
cgcgagcgt cagttggcg gccgggggt tcacaggaa 
gagtccagg gttctggat ttgagcaaa cctgggtgt 
agttacacc ggggagcag aagagtaga cgcaaaggg 
ccggcgttt aaggtcgat ccgactcaa gtcgagtcg 
gaggaggag gacgaggag acgtccacc actgtgttt 
agtaacgtg gaggtcgtc caagaggag gcgaagaag 
gaggagtct gatagtaat aataacaat aacaaggag 
gagggtaac agttgccaa tattgtaat atcgcgttc 
ggggacgcc gtcctctac accatccat atgggctac 
cacgggttc cacaacccg ttcacttgc aacatgtgc 
ggggtggaa tgctccgat aaagtatcg ttctttttg 
catattgca cgcgtttct cattcgtaa
    • >TC013553 RB
      atggctatc tgtgatttg gcatggtcc gaaacttat 
ttttcattc atgttcgag cggggacgt agtgtagtg 
tctctcaaa tttaatcat tgtttttta tcgagtgta 
gtattttta aaaaactgt tcgagtgca tgtgaaatg 
atagataaa gacatgaat tcggcttgc atgaggggt 
ggctcagtc cggacttta aacaattac caacaagtg 
atggaaccg cggtccccc cacaccgcc tggcaattc 
ggcgtttcc cagatcgtg aaacgcgaa ccaatggac 
gaagataag aacgattcc ggggttacc tccggaagc 
gacttccat tcatcctcc cccagcagt gatacgagc 
caggattta cagcactct tatcaatcg ccgcaaacg 
caaccggcc cgcttctac tccactccc atcgtgccc 
cacttcgcc tacaaccac aacccccta acacccccc 
aactccgag ccactggtc tcccccaag agcgagaag 
gaggagaag gacatggag acgaccctc acgccttgc 
gcgtccccg aaccgcaag ccggacgac aatcaggac 
cacctccgc cgcctggag atgtccctg gagaagagc 
ggcctgttc tcgtccaaa acgagcgaa cactccgtg 
gatgagttg tcggggaag agcgacaac gacgccgag 
gagtacgac gagcagagc ttgcgcgtc ccgaaagtc 
aactcgcac gggaaaatc aaaactttc aaatgcaaa 
cagtgcgat tttgtcgca atcaccaaa ctcgaacag 
tggaaccac agcaaggtg cacatccgg gaggacaag 
cggctgacc tgccccaag tgcccgttc atcaccgag 
tacaagcac cacctggag taccacctc cgcaaccac 
gccgggtcc aaaccgttc caatgcaac aaatgcgac 
tacacttgt gtgaacaag tccatgcta aactcacac 
atgaagtcg cactcgaac gtctaccgt tatagttgc 
cgggattgc agctacgct accaaatat tgccactct 
ttgaagatc cacctgagg cggtatgga cacaccccg 
aacgtggtt ttggacgag gaaggaaac ccgtgtccg 
gacatcatc atcgacgtg catgggacg cgtcggggt 
cccaaaatt aagacacaa cctaaagcc gaagaggcg 
aaaccggaa acgctcccg ttccttaac ctgcaacaa 
caactacct ttccctggt tatcccttc tttgggggg 
ttccccaac gcccagttg ttgcaacaa ctgatccgc 
gagcgtcag ttggcggcc gggggttca caggaagag 
tccagggtt ctggatttg agcaaacct gggtgtagt 
tacaccggg gagcagaag agtagacgc aaagggccg 
gcgtttaag gtcgatccg actcaagtc gagtcggag 
gaggaggac gaggagacg tccaccact gtgtttagt 
aacgtggag gtcgtccaa gaggaggcg aagaaggag 
gagtctgat agtaataat aacaataac aaggaggag 
ggtaacagt tgccaatat tgtaatatc gcgttcggg 
gacgccgtc ctctacacc atccatatg ggctaccac 
gggttccac aacccgttc acttgcaac atgtgcggg 
gtggaatgc tccgataaa gtatcgttc tttttgcat 
attgcacgc gtttctcat tcgtaa
    mRNA sequence
    • >TC013553 RA
      aaaccactg ataaaatca cattcagtc tttgacatt 
cctaaaaga cacttcaaa gtttgttgt ttgtgtgca 
aaaatttcg aacagtcgg gtgcatgtg aaatgatag 
ataaagaca tgaattcgg cttgcatga ggggtggct 
cagtccgga ctttaaaca attaccaac aagtgatgg 
aaccgcggt ccccccaca ccgcctggc aattcggcg 
tttcccaga tcgtgaaac gcgaaccaa tggacgaag 
ataagaacg attccgggg ttacctccg gaagcgact 
tccattcat cctccccca gcagtgata cgagccagg 
atttacagc actcttatc aatcgccgc aaacgcaac 
cggcccgct tctactcca ctcccatcg tgccccact 
tcgcctaca accacaacc ccctaacac cccccaact 
ccgagccac tggtctccc ccaagagcg agaaggagg 
agaaggaca tggagacga ccctcacgc cttgcgcgt 
ccccgaacc gcaagccgg acgacaatc aggaccacc 
tccgccgcc tggagatgt ccctggaga agagcggcc 
tgttctcgt ccaaaacga gcgaacact ccgtggatg 
agttgtcgg ggaagagcg acaacgacg ccgaggagt 
acgacgagc agagcttgc gcgtcccga aagtcaact 
cgcacggga aaatcaaaa ctttcaaat gcaaacagt 
gcgattttg tcgcaatca ccaaactcg aacagtgga 
accacagca aggtgcaca tccgggagg acaagcggc 
tgacctgcc ccaagtgcc cgttcatca ccgagtaca 
agcaccacc tggagtacc acctccgca accacgccg 
ggtccaaac cgttccaat gcaacaaat gcgactaca 
cttgtgtga acaagtcca tgctaaact cacacatga 
agtcgcact cgaacgtct accgttata gttgccggg 
attgcagct acgctacca aatattgcc actctttga 
agatccacc tgaggcggt atggacaca ccccgaacg 
tggttttgg acgaggaag gaaacccgt gtccggaca 
tcatcatcg acgtgcatg ggacgcgtc ggggtccca 
aaattaaga cacaaccta aagccgaag aggcgaaac 
cggaaacgc tcccgttcc ttaacctgc aacaacaac 
tacctttcc ctggttatc ccttctttg gggggttcc 
ccaacgccc agttgttgc aacaactga tccgcgagc 
gtcagttgg cggccgggg gttcacagg aagagtcca 
gggttctgg atttgagca aacctgggt gtagttaca 
ccggggagc agaagagta gacgcaaag ggccggcgt 
ttaaggtcg atccgactc aagtcgagt cggaggagg 
aggacgagg agacgtcca ccactgtgt ttagtaacg 
tggaggtcg tccaagagg aggcgaaga aggaggagt 
ctgatagta ataataaca ataacaagg aggagggta 
acagttgcc aatattgta atatcgcgt tcggggacg 
ccgtcctct acaccatcc atatgggct accacgggt 
tccacaacc cgttcactt gcaacatgt gcggggtgg 
aatgctccg ataaagtat cgttctttt tgcatattg 
cacgcgttt ctcattcgt aaattaaga ctgattaga 
gttaactag acttaggtt tgtgttttt tatttaact 
agtcatacg gggccgcgg cccgatttc tcaatctag 
ttattttac gcttttacg tgcaattta tttttgtta 
accacaaat ggagatgta cgggttgga actccacag 
ctgaaccaa atattttct ttcgccgaa ttaggtcaa 
tttttaatt tatttttgt ctgtaacgt tttgtcgta 
gtggtttta gtaaatagt cgagtttta ttgtcatat 
ttttatact tgtggggcc atgacataa gactgatga 
ttgtatttg taaataagt ttcaatgtt ttctatagc 
tcgagcaac aatcaaaga ttattgtaa catagttgt 
gtgtattat acctactat tgtttaagc caaataata 
aacagtagc agagcaa
    • >TC013553 RB
      aatcgtgga gcttttgaa agcgcccga tggagggga 
tgtctgtat tggaggggt gaagtcaag ccgtttggg 
gtgggagga actggacat ggctatctg tgatttggc 
atggtccga aacttattt ttcattcat gttcgagcg 
gggacgtag tgtagtgtc tctcaaatt taatcattg 
ttttttatc gagtgtagt atttttaaa aaactgttc 
gagtgcatg tgaaatgat agataaaga catgaattc 
ggcttgcat gaggggtgg ctcagtccg gactttaaa 
caattacca acaagtgat ggaaccgcg gtcccccca 
caccgcctg gcaattcgg cgtttccca gatcgtgaa 
acgcgaacc aatggacga agataagaa cgattccgg 
ggttacctc cggaagcga cttccattc atcctcccc 
cagcagtga tacgagcca ggatttaca gcactctta 
tcaatcgcc gcaaacgca accggcccg cttctactc 
cactcccat cgtgcccca cttcgccta caaccacaa 
ccccctaac accccccaa ctccgagcc actggtctc 
ccccaagag cgagaagga ggagaagga catggagac 
gaccctcac gccttgcgc gtccccgaa ccgcaagcc 
ggacgacaa tcaggacca cctccgccg cctggagat 
gtccctgga gaagagcgg cctgttctc gtccaaaac 
gagcgaaca ctccgtgga tgagttgtc ggggaagag 
cgacaacga cgccgagga gtacgacga gcagagctt 
gcgcgtccc gaaagtcaa ctcgcacgg gaaaatcaa 
aactttcaa atgcaaaca gtgcgattt tgtcgcaat 
caccaaact cgaacagtg gaaccacag caaggtgca 
catccggga ggacaagcg gctgacctg ccccaagtg 
cccgttcat caccgagta caagcacca cctggagta 
ccacctccg caaccacgc cgggtccaa accgttcca 
atgcaacaa atgcgacta cacttgtgt gaacaagtc 
catgctaaa ctcacacat gaagtcgca ctcgaacgt 
ctaccgtta tagttgccg ggattgcag ctacgctac 
caaatattg ccactcttt gaagatcca cctgaggcg 
gtatggaca caccccgaa cgtggtttt ggacgagga 
aggaaaccc gtgtccgga catcatcat cgacgtgca 
tgggacgcg tcggggtcc caaaattaa gacacaacc 
taaagccga agaggcgaa accggaaac gctcccgtt 
ccttaacct gcaacaaca actaccttt ccctggtta 
tcccttctt tggggggtt ccccaacgc ccagttgtt 
gcaacaact gatccgcga gcgtcagtt ggcggccgg 
gggttcaca ggaagagtc cagggttct ggatttgag 
caaacctgg gtgtagtta caccgggga gcagaagag 
tagacgcaa agggccggc gtttaaggt cgatccgac 
tcaagtcga gtcggagga ggaggacga ggagacgtc 
caccactgt gtttagtaa cgtggaggt cgtccaaga 
ggaggcgaa gaaggagga gtctgatag taataataa 
caataacaa ggaggaggg taacagttg ccaatattg 
taatatcgc gttcgggga cgccgtcct ctacaccat 
ccatatggg ctaccacgg gttccacaa cccgttcac 
ttgcaacat gtgcggggt ggaatgctc cgataaagt 
atcgttctt tttgcatat tgcacgcgt ttctcattc 
gtaaattaa gactgatta gagttaact agacttagg 
tttgtgttt tttatttaa ctagtcata cggggccgc 
ggcccgatt tctcaatct agttatttt acgctttta 
cgtgcaatt tatttttgt taaccacaa atggagatg 
tacgggttg gaactccac agctgaacc aaatatttt 
ctttcgccg aattaggtc aatttttaa tttattttt 
gtctgtaac gttttgtcg tagtggttt tagtaaata 
gtcgagttt tattgtcat atttttata cttgtgggg 
ccatgacat aagactgat gattgtatt tgtaaataa 
gtttcaatg ttttctata gctcgagca acaatcaaa 
gattattgt aacatagtt gtgtgtatt atacctact 
attgtttaa gccaaataa taaacagta gcagagcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY