General information | |||
TC013553 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8101361..8105747 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC013553 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05451 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
mandible size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
maxilla not present - Penetrance (percentage of animals/eggs on slide): more than 80%
labium not present - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen segment number # of remaining segments decreased -
posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC013553 RA
MIDKDMNSA CMRGGSVRT LNNYQQVME PRSPHTAWQ FGVSQIVKR EPMDEDKND SGVTSGSDF HSSSPSSDT SQDLQHSYQ SPQTQPARF YSTPIVPHF AYNHNPLTP PNSEPLVSP KSEKEEKDM ETTLTPCAS PNRKPDDNQ DHLRRLEMS LEKSGLFSS KTSEHSVDE LSGKSDNDA EEYDEQSLR VPKVNSHGK IKTFKCKQC DFVAITKLE QWNHSKVHI REDKRLTCP KCPFITEYK HHLEYHLRN HAGSKPFQC NKCDYTCVN KSMLNSHMK SHSNVYRYS CRDCSYATK YCHSLKIHL RRYGHTPNV VLDEEGNPC PDIIIDVHG TRRGPKIKT QPKAEEAKP ETLPFLNLQ QQLPFPGYP FFGGFPNAQ LLQQLIRER QLAAGGSQE ESRVLDLSK PGCSYTGEQ KSRRKGPAF KVDPTQVES EEEDEETST TVFSNVEVV QEEAKKEES DSNNNNNKE EGNSCQYCN IAFGDAVLY TIHMGYHGF HNPFTCNMC GVECSDKVS FFLHIARVS HS
- >TC013553 RB
MAICDLAWS ETYFSFMFE RGRSVVSLK FNHCFLSSV VFLKNCSSA CEMIDKDMN SACMRGGSV RTLNNYQQV MEPRSPHTA WQFGVSQIV KREPMDEDK NDSGVTSGS DFHSSSPSS DTSQDLQHS YQSPQTQPA RFYSTPIVP HFAYNHNPL TPPNSEPLV SPKSEKEEK DMETTLTPC ASPNRKPDD NQDHLRRLE MSLEKSGLF SSKTSEHSV DELSGKSDN DAEEYDEQS LRVPKVNSH GKIKTFKCK QCDFVAITK LEQWNHSKV HIREDKRLT CPKCPFITE YKHHLEYHL RNHAGSKPF QCNKCDYTC VNKSMLNSH MKSHSNVYR YSCRDCSYA TKYCHSLKI HLRRYGHTP NVVLDEEGN PCPDIIIDV HGTRRGPKI KTQPKAEEA KPETLPFLN LQQQLPFPG YPFFGGFPN AQLLQQLIR ERQLAAGGS QEESRVLDL SKPGCSYTG EQKSRRKGP AFKVDPTQV ESEEEDEET STTVFSNVE VVQEEAKKE ESDSNNNNN KEEGNSCQY CNIAFGDAV LYTIHMGYH GFHNPFTCN MCGVECSDK VSFFLHIAR VSHS
- >TC013553 RA
atgatagat aaagacatg aattcggct tgcatgagg ggtggctca gtccggact ttaaacaat taccaacaa gtgatggaa ccgcggtcc ccccacacc gcctggcaa ttcggcgtt tcccagatc gtgaaacgc gaaccaatg gacgaagat aagaacgat tccggggtt acctccgga agcgacttc cattcatcc tcccccagc agtgatacg agccaggat ttacagcac tcttatcaa tcgccgcaa acgcaaccg gcccgcttc tactccact cccatcgtg ccccacttc gcctacaac cacaacccc ctaacaccc cccaactcc gagccactg gtctccccc aagagcgag aaggaggag aaggacatg gagacgacc ctcacgcct tgcgcgtcc ccgaaccgc aagccggac gacaatcag gaccacctc cgccgcctg gagatgtcc ctggagaag agcggcctg ttctcgtcc aaaacgagc gaacactcc gtggatgag ttgtcgggg aagagcgac aacgacgcc gaggagtac gacgagcag agcttgcgc gtcccgaaa gtcaactcg cacgggaaa atcaaaact ttcaaatgc aaacagtgc gattttgtc gcaatcacc aaactcgaa cagtggaac cacagcaag gtgcacatc cgggaggac aagcggctg acctgcccc aagtgcccg ttcatcacc gagtacaag caccacctg gagtaccac ctccgcaac cacgccggg tccaaaccg ttccaatgc aacaaatgc gactacact tgtgtgaac aagtccatg ctaaactca cacatgaag tcgcactcg aacgtctac cgttatagt tgccgggat tgcagctac gctaccaaa tattgccac tctttgaag atccacctg aggcggtat ggacacacc ccgaacgtg gttttggac gaggaagga aacccgtgt ccggacatc atcatcgac gtgcatggg acgcgtcgg ggtcccaaa attaagaca caacctaaa gccgaagag gcgaaaccg gaaacgctc ccgttcctt aacctgcaa caacaacta cctttccct ggttatccc ttctttggg gggttcccc aacgcccag ttgttgcaa caactgatc cgcgagcgt cagttggcg gccgggggt tcacaggaa gagtccagg gttctggat ttgagcaaa cctgggtgt agttacacc ggggagcag aagagtaga cgcaaaggg ccggcgttt aaggtcgat ccgactcaa gtcgagtcg gaggaggag gacgaggag acgtccacc actgtgttt agtaacgtg gaggtcgtc caagaggag gcgaagaag gaggagtct gatagtaat aataacaat aacaaggag gagggtaac agttgccaa tattgtaat atcgcgttc ggggacgcc gtcctctac accatccat atgggctac cacgggttc cacaacccg ttcacttgc aacatgtgc ggggtggaa tgctccgat aaagtatcg ttctttttg catattgca cgcgtttct cattcgtaa
- >TC013553 RB
atggctatc tgtgatttg gcatggtcc gaaacttat ttttcattc atgttcgag cggggacgt agtgtagtg tctctcaaa tttaatcat tgtttttta tcgagtgta gtattttta aaaaactgt tcgagtgca tgtgaaatg atagataaa gacatgaat tcggcttgc atgaggggt ggctcagtc cggacttta aacaattac caacaagtg atggaaccg cggtccccc cacaccgcc tggcaattc ggcgtttcc cagatcgtg aaacgcgaa ccaatggac gaagataag aacgattcc ggggttacc tccggaagc gacttccat tcatcctcc cccagcagt gatacgagc caggattta cagcactct tatcaatcg ccgcaaacg caaccggcc cgcttctac tccactccc atcgtgccc cacttcgcc tacaaccac aacccccta acacccccc aactccgag ccactggtc tcccccaag agcgagaag gaggagaag gacatggag acgaccctc acgccttgc gcgtccccg aaccgcaag ccggacgac aatcaggac cacctccgc cgcctggag atgtccctg gagaagagc ggcctgttc tcgtccaaa acgagcgaa cactccgtg gatgagttg tcggggaag agcgacaac gacgccgag gagtacgac gagcagagc ttgcgcgtc ccgaaagtc aactcgcac gggaaaatc aaaactttc aaatgcaaa cagtgcgat tttgtcgca atcaccaaa ctcgaacag tggaaccac agcaaggtg cacatccgg gaggacaag cggctgacc tgccccaag tgcccgttc atcaccgag tacaagcac cacctggag taccacctc cgcaaccac gccgggtcc aaaccgttc caatgcaac aaatgcgac tacacttgt gtgaacaag tccatgcta aactcacac atgaagtcg cactcgaac gtctaccgt tatagttgc cgggattgc agctacgct accaaatat tgccactct ttgaagatc cacctgagg cggtatgga cacaccccg aacgtggtt ttggacgag gaaggaaac ccgtgtccg gacatcatc atcgacgtg catgggacg cgtcggggt cccaaaatt aagacacaa cctaaagcc gaagaggcg aaaccggaa acgctcccg ttccttaac ctgcaacaa caactacct ttccctggt tatcccttc tttgggggg ttccccaac gcccagttg ttgcaacaa ctgatccgc gagcgtcag ttggcggcc gggggttca caggaagag tccagggtt ctggatttg agcaaacct gggtgtagt tacaccggg gagcagaag agtagacgc aaagggccg gcgtttaag gtcgatccg actcaagtc gagtcggag gaggaggac gaggagacg tccaccact gtgtttagt aacgtggag gtcgtccaa gaggaggcg aagaaggag gagtctgat agtaataat aacaataac aaggaggag ggtaacagt tgccaatat tgtaatatc gcgttcggg gacgccgtc ctctacacc atccatatg ggctaccac gggttccac aacccgttc acttgcaac atgtgcggg gtggaatgc tccgataaa gtatcgttc tttttgcat attgcacgc gtttctcat tcgtaa
- >TC013553 RA
aaaccactg ataaaatca cattcagtc tttgacatt cctaaaaga cacttcaaa gtttgttgt ttgtgtgca aaaatttcg aacagtcgg gtgcatgtg aaatgatag ataaagaca tgaattcgg cttgcatga ggggtggct cagtccgga ctttaaaca attaccaac aagtgatgg aaccgcggt ccccccaca ccgcctggc aattcggcg tttcccaga tcgtgaaac gcgaaccaa tggacgaag ataagaacg attccgggg ttacctccg gaagcgact tccattcat cctccccca gcagtgata cgagccagg atttacagc actcttatc aatcgccgc aaacgcaac cggcccgct tctactcca ctcccatcg tgccccact tcgcctaca accacaacc ccctaacac cccccaact ccgagccac tggtctccc ccaagagcg agaaggagg agaaggaca tggagacga ccctcacgc cttgcgcgt ccccgaacc gcaagccgg acgacaatc aggaccacc tccgccgcc tggagatgt ccctggaga agagcggcc tgttctcgt ccaaaacga gcgaacact ccgtggatg agttgtcgg ggaagagcg acaacgacg ccgaggagt acgacgagc agagcttgc gcgtcccga aagtcaact cgcacggga aaatcaaaa ctttcaaat gcaaacagt gcgattttg tcgcaatca ccaaactcg aacagtgga accacagca aggtgcaca tccgggagg acaagcggc tgacctgcc ccaagtgcc cgttcatca ccgagtaca agcaccacc tggagtacc acctccgca accacgccg ggtccaaac cgttccaat gcaacaaat gcgactaca cttgtgtga acaagtcca tgctaaact cacacatga agtcgcact cgaacgtct accgttata gttgccggg attgcagct acgctacca aatattgcc actctttga agatccacc tgaggcggt atggacaca ccccgaacg tggttttgg acgaggaag gaaacccgt gtccggaca tcatcatcg acgtgcatg ggacgcgtc ggggtccca aaattaaga cacaaccta aagccgaag aggcgaaac cggaaacgc tcccgttcc ttaacctgc aacaacaac tacctttcc ctggttatc ccttctttg gggggttcc ccaacgccc agttgttgc aacaactga tccgcgagc gtcagttgg cggccgggg gttcacagg aagagtcca gggttctgg atttgagca aacctgggt gtagttaca ccggggagc agaagagta gacgcaaag ggccggcgt ttaaggtcg atccgactc aagtcgagt cggaggagg aggacgagg agacgtcca ccactgtgt ttagtaacg tggaggtcg tccaagagg aggcgaaga aggaggagt ctgatagta ataataaca ataacaagg aggagggta acagttgcc aatattgta atatcgcgt tcggggacg ccgtcctct acaccatcc atatgggct accacgggt tccacaacc cgttcactt gcaacatgt gcggggtgg aatgctccg ataaagtat cgttctttt tgcatattg cacgcgttt ctcattcgt aaattaaga ctgattaga gttaactag acttaggtt tgtgttttt tatttaact agtcatacg gggccgcgg cccgatttc tcaatctag ttattttac gcttttacg tgcaattta tttttgtta accacaaat ggagatgta cgggttgga actccacag ctgaaccaa atattttct ttcgccgaa ttaggtcaa tttttaatt tatttttgt ctgtaacgt tttgtcgta gtggtttta gtaaatagt cgagtttta ttgtcatat ttttatact tgtggggcc atgacataa gactgatga ttgtatttg taaataagt ttcaatgtt ttctatagc tcgagcaac aatcaaaga ttattgtaa catagttgt gtgtattat acctactat tgtttaagc caaataata aacagtagc agagcaa
- >TC013553 RB
aatcgtgga gcttttgaa agcgcccga tggagggga tgtctgtat tggaggggt gaagtcaag ccgtttggg gtgggagga actggacat ggctatctg tgatttggc atggtccga aacttattt ttcattcat gttcgagcg gggacgtag tgtagtgtc tctcaaatt taatcattg ttttttatc gagtgtagt atttttaaa aaactgttc gagtgcatg tgaaatgat agataaaga catgaattc ggcttgcat gaggggtgg ctcagtccg gactttaaa caattacca acaagtgat ggaaccgcg gtcccccca caccgcctg gcaattcgg cgtttccca gatcgtgaa acgcgaacc aatggacga agataagaa cgattccgg ggttacctc cggaagcga cttccattc atcctcccc cagcagtga tacgagcca ggatttaca gcactctta tcaatcgcc gcaaacgca accggcccg cttctactc cactcccat cgtgcccca cttcgccta caaccacaa ccccctaac accccccaa ctccgagcc actggtctc ccccaagag cgagaagga ggagaagga catggagac gaccctcac gccttgcgc gtccccgaa ccgcaagcc ggacgacaa tcaggacca cctccgccg cctggagat gtccctgga gaagagcgg cctgttctc gtccaaaac gagcgaaca ctccgtgga tgagttgtc ggggaagag cgacaacga cgccgagga gtacgacga gcagagctt gcgcgtccc gaaagtcaa ctcgcacgg gaaaatcaa aactttcaa atgcaaaca gtgcgattt tgtcgcaat caccaaact cgaacagtg gaaccacag caaggtgca catccggga ggacaagcg gctgacctg ccccaagtg cccgttcat caccgagta caagcacca cctggagta ccacctccg caaccacgc cgggtccaa accgttcca atgcaacaa atgcgacta cacttgtgt gaacaagtc catgctaaa ctcacacat gaagtcgca ctcgaacgt ctaccgtta tagttgccg ggattgcag ctacgctac caaatattg ccactcttt gaagatcca cctgaggcg gtatggaca caccccgaa cgtggtttt ggacgagga aggaaaccc gtgtccgga catcatcat cgacgtgca tgggacgcg tcggggtcc caaaattaa gacacaacc taaagccga agaggcgaa accggaaac gctcccgtt ccttaacct gcaacaaca actaccttt ccctggtta tcccttctt tggggggtt ccccaacgc ccagttgtt gcaacaact gatccgcga gcgtcagtt ggcggccgg gggttcaca ggaagagtc cagggttct ggatttgag caaacctgg gtgtagtta caccgggga gcagaagag tagacgcaa agggccggc gtttaaggt cgatccgac tcaagtcga gtcggagga ggaggacga ggagacgtc caccactgt gtttagtaa cgtggaggt cgtccaaga ggaggcgaa gaaggagga gtctgatag taataataa caataacaa ggaggaggg taacagttg ccaatattg taatatcgc gttcgggga cgccgtcct ctacaccat ccatatggg ctaccacgg gttccacaa cccgttcac ttgcaacat gtgcggggt ggaatgctc cgataaagt atcgttctt tttgcatat tgcacgcgt ttctcattc gtaaattaa gactgatta gagttaact agacttagg tttgtgttt tttatttaa ctagtcata cggggccgc ggcccgatt tctcaatct agttatttt acgctttta cgtgcaatt tatttttgt taaccacaa atggagatg tacgggttg gaactccac agctgaacc aaatatttt ctttcgccg aattaggtc aatttttaa tttattttt gtctgtaac gttttgtcg tagtggttt tagtaaata gtcgagttt tattgtcat atttttata cttgtgggg ccatgacat aagactgat gattgtatt tgtaaataa gtttcaatg ttttctata gctcgagca acaatcaaa gattattgt aacatagtt gtgtgtatt atacctact attgtttaa gccaaataa taaacagta gcagagcaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
