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Gene information
General information
TC013553 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8101361..8105747
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013553
    iBeetle screen iB_05451

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    mandible size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    maxilla not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    labium not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number # of remaining segments decreased -
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013553 RA
      MIDKDMNSA CMRGGSVRT LNNYQQVME PRSPHTAWQ 
      FGVSQIVKR EPMDEDKND SGVTSGSDF HSSSPSSDT 
      SQDLQHSYQ SPQTQPARF YSTPIVPHF AYNHNPLTP 
      PNSEPLVSP KSEKEEKDM ETTLTPCAS PNRKPDDNQ 
      DHLRRLEMS LEKSGLFSS KTSEHSVDE LSGKSDNDA 
      EEYDEQSLR VPKVNSHGK IKTFKCKQC DFVAITKLE 
      QWNHSKVHI REDKRLTCP KCPFITEYK HHLEYHLRN 
      HAGSKPFQC NKCDYTCVN KSMLNSHMK SHSNVYRYS 
      CRDCSYATK YCHSLKIHL RRYGHTPNV VLDEEGNPC 
      PDIIIDVHG TRRGPKIKT QPKAEEAKP ETLPFLNLQ 
      QQLPFPGYP FFGGFPNAQ LLQQLIRER QLAAGGSQE 
      ESRVLDLSK PGCSYTGEQ KSRRKGPAF KVDPTQVES 
      EEEDEETST TVFSNVEVV QEEAKKEES DSNNNNNKE 
      EGNSCQYCN IAFGDAVLY TIHMGYHGF HNPFTCNMC 
      GVECSDKVS FFLHIARVS HS
    • >TC013553 RB
      MAICDLAWS ETYFSFMFE RGRSVVSLK FNHCFLSSV 
      VFLKNCSSA CEMIDKDMN SACMRGGSV RTLNNYQQV 
      MEPRSPHTA WQFGVSQIV KREPMDEDK NDSGVTSGS 
      DFHSSSPSS DTSQDLQHS YQSPQTQPA RFYSTPIVP 
      HFAYNHNPL TPPNSEPLV SPKSEKEEK DMETTLTPC 
      ASPNRKPDD NQDHLRRLE MSLEKSGLF SSKTSEHSV 
      DELSGKSDN DAEEYDEQS LRVPKVNSH GKIKTFKCK 
      QCDFVAITK LEQWNHSKV HIREDKRLT CPKCPFITE 
      YKHHLEYHL RNHAGSKPF QCNKCDYTC VNKSMLNSH 
      MKSHSNVYR YSCRDCSYA TKYCHSLKI HLRRYGHTP 
      NVVLDEEGN PCPDIIIDV HGTRRGPKI KTQPKAEEA 
      KPETLPFLN LQQQLPFPG YPFFGGFPN AQLLQQLIR 
      ERQLAAGGS QEESRVLDL SKPGCSYTG EQKSRRKGP 
      AFKVDPTQV ESEEEDEET STTVFSNVE VVQEEAKKE 
      ESDSNNNNN KEEGNSCQY CNIAFGDAV LYTIHMGYH 
      GFHNPFTCN MCGVECSDK VSFFLHIAR VSHS
    CDS sequence
    • >TC013553 RA
      atgatagat aaagacatg aattcggct tgcatgagg 
      ggtggctca gtccggact ttaaacaat taccaacaa 
      gtgatggaa ccgcggtcc ccccacacc gcctggcaa 
      ttcggcgtt tcccagatc gtgaaacgc gaaccaatg 
      gacgaagat aagaacgat tccggggtt acctccgga 
      agcgacttc cattcatcc tcccccagc agtgatacg 
      agccaggat ttacagcac tcttatcaa tcgccgcaa 
      acgcaaccg gcccgcttc tactccact cccatcgtg 
      ccccacttc gcctacaac cacaacccc ctaacaccc 
      cccaactcc gagccactg gtctccccc aagagcgag 
      aaggaggag aaggacatg gagacgacc ctcacgcct 
      tgcgcgtcc ccgaaccgc aagccggac gacaatcag 
      gaccacctc cgccgcctg gagatgtcc ctggagaag 
      agcggcctg ttctcgtcc aaaacgagc gaacactcc 
      gtggatgag ttgtcgggg aagagcgac aacgacgcc 
      gaggagtac gacgagcag agcttgcgc gtcccgaaa 
      gtcaactcg cacgggaaa atcaaaact ttcaaatgc 
      aaacagtgc gattttgtc gcaatcacc aaactcgaa 
      cagtggaac cacagcaag gtgcacatc cgggaggac 
      aagcggctg acctgcccc aagtgcccg ttcatcacc 
      gagtacaag caccacctg gagtaccac ctccgcaac 
      cacgccggg tccaaaccg ttccaatgc aacaaatgc 
      gactacact tgtgtgaac aagtccatg ctaaactca 
      cacatgaag tcgcactcg aacgtctac cgttatagt 
      tgccgggat tgcagctac gctaccaaa tattgccac 
      tctttgaag atccacctg aggcggtat ggacacacc 
      ccgaacgtg gttttggac gaggaagga aacccgtgt 
      ccggacatc atcatcgac gtgcatggg acgcgtcgg 
      ggtcccaaa attaagaca caacctaaa gccgaagag 
      gcgaaaccg gaaacgctc ccgttcctt aacctgcaa 
      caacaacta cctttccct ggttatccc ttctttggg 
      gggttcccc aacgcccag ttgttgcaa caactgatc 
      cgcgagcgt cagttggcg gccgggggt tcacaggaa 
      gagtccagg gttctggat ttgagcaaa cctgggtgt 
      agttacacc ggggagcag aagagtaga cgcaaaggg 
      ccggcgttt aaggtcgat ccgactcaa gtcgagtcg 
      gaggaggag gacgaggag acgtccacc actgtgttt 
      agtaacgtg gaggtcgtc caagaggag gcgaagaag 
      gaggagtct gatagtaat aataacaat aacaaggag 
      gagggtaac agttgccaa tattgtaat atcgcgttc 
      ggggacgcc gtcctctac accatccat atgggctac 
      cacgggttc cacaacccg ttcacttgc aacatgtgc 
      ggggtggaa tgctccgat aaagtatcg ttctttttg 
      catattgca cgcgtttct cattcgtaa
    • >TC013553 RB
      atggctatc tgtgatttg gcatggtcc gaaacttat 
      ttttcattc atgttcgag cggggacgt agtgtagtg 
      tctctcaaa tttaatcat tgtttttta tcgagtgta 
      gtattttta aaaaactgt tcgagtgca tgtgaaatg 
      atagataaa gacatgaat tcggcttgc atgaggggt 
      ggctcagtc cggacttta aacaattac caacaagtg 
      atggaaccg cggtccccc cacaccgcc tggcaattc 
      ggcgtttcc cagatcgtg aaacgcgaa ccaatggac 
      gaagataag aacgattcc ggggttacc tccggaagc 
      gacttccat tcatcctcc cccagcagt gatacgagc 
      caggattta cagcactct tatcaatcg ccgcaaacg 
      caaccggcc cgcttctac tccactccc atcgtgccc 
      cacttcgcc tacaaccac aacccccta acacccccc 
      aactccgag ccactggtc tcccccaag agcgagaag 
      gaggagaag gacatggag acgaccctc acgccttgc 
      gcgtccccg aaccgcaag ccggacgac aatcaggac 
      cacctccgc cgcctggag atgtccctg gagaagagc 
      ggcctgttc tcgtccaaa acgagcgaa cactccgtg 
      gatgagttg tcggggaag agcgacaac gacgccgag 
      gagtacgac gagcagagc ttgcgcgtc ccgaaagtc 
      aactcgcac gggaaaatc aaaactttc aaatgcaaa 
      cagtgcgat tttgtcgca atcaccaaa ctcgaacag 
      tggaaccac agcaaggtg cacatccgg gaggacaag 
      cggctgacc tgccccaag tgcccgttc atcaccgag 
      tacaagcac cacctggag taccacctc cgcaaccac 
      gccgggtcc aaaccgttc caatgcaac aaatgcgac 
      tacacttgt gtgaacaag tccatgcta aactcacac 
      atgaagtcg cactcgaac gtctaccgt tatagttgc 
      cgggattgc agctacgct accaaatat tgccactct 
      ttgaagatc cacctgagg cggtatgga cacaccccg 
      aacgtggtt ttggacgag gaaggaaac ccgtgtccg 
      gacatcatc atcgacgtg catgggacg cgtcggggt 
      cccaaaatt aagacacaa cctaaagcc gaagaggcg 
      aaaccggaa acgctcccg ttccttaac ctgcaacaa 
      caactacct ttccctggt tatcccttc tttgggggg 
      ttccccaac gcccagttg ttgcaacaa ctgatccgc 
      gagcgtcag ttggcggcc gggggttca caggaagag 
      tccagggtt ctggatttg agcaaacct gggtgtagt 
      tacaccggg gagcagaag agtagacgc aaagggccg 
      gcgtttaag gtcgatccg actcaagtc gagtcggag 
      gaggaggac gaggagacg tccaccact gtgtttagt 
      aacgtggag gtcgtccaa gaggaggcg aagaaggag 
      gagtctgat agtaataat aacaataac aaggaggag 
      ggtaacagt tgccaatat tgtaatatc gcgttcggg 
      gacgccgtc ctctacacc atccatatg ggctaccac 
      gggttccac aacccgttc acttgcaac atgtgcggg 
      gtggaatgc tccgataaa gtatcgttc tttttgcat 
      attgcacgc gtttctcat tcgtaa
    mRNA sequence
    • >TC013553 RA
      aaaccactg ataaaatca cattcagtc tttgacatt 
      cctaaaaga cacttcaaa gtttgttgt ttgtgtgca 
      aaaatttcg aacagtcgg gtgcatgtg aaatgatag 
      ataaagaca tgaattcgg cttgcatga ggggtggct 
      cagtccgga ctttaaaca attaccaac aagtgatgg 
      aaccgcggt ccccccaca ccgcctggc aattcggcg 
      tttcccaga tcgtgaaac gcgaaccaa tggacgaag 
      ataagaacg attccgggg ttacctccg gaagcgact 
      tccattcat cctccccca gcagtgata cgagccagg 
      atttacagc actcttatc aatcgccgc aaacgcaac 
      cggcccgct tctactcca ctcccatcg tgccccact 
      tcgcctaca accacaacc ccctaacac cccccaact 
      ccgagccac tggtctccc ccaagagcg agaaggagg 
      agaaggaca tggagacga ccctcacgc cttgcgcgt 
      ccccgaacc gcaagccgg acgacaatc aggaccacc 
      tccgccgcc tggagatgt ccctggaga agagcggcc 
      tgttctcgt ccaaaacga gcgaacact ccgtggatg 
      agttgtcgg ggaagagcg acaacgacg ccgaggagt 
      acgacgagc agagcttgc gcgtcccga aagtcaact 
      cgcacggga aaatcaaaa ctttcaaat gcaaacagt 
      gcgattttg tcgcaatca ccaaactcg aacagtgga 
      accacagca aggtgcaca tccgggagg acaagcggc 
      tgacctgcc ccaagtgcc cgttcatca ccgagtaca 
      agcaccacc tggagtacc acctccgca accacgccg 
      ggtccaaac cgttccaat gcaacaaat gcgactaca 
      cttgtgtga acaagtcca tgctaaact cacacatga 
      agtcgcact cgaacgtct accgttata gttgccggg 
      attgcagct acgctacca aatattgcc actctttga 
      agatccacc tgaggcggt atggacaca ccccgaacg 
      tggttttgg acgaggaag gaaacccgt gtccggaca 
      tcatcatcg acgtgcatg ggacgcgtc ggggtccca 
      aaattaaga cacaaccta aagccgaag aggcgaaac 
      cggaaacgc tcccgttcc ttaacctgc aacaacaac 
      tacctttcc ctggttatc ccttctttg gggggttcc 
      ccaacgccc agttgttgc aacaactga tccgcgagc 
      gtcagttgg cggccgggg gttcacagg aagagtcca 
      gggttctgg atttgagca aacctgggt gtagttaca 
      ccggggagc agaagagta gacgcaaag ggccggcgt 
      ttaaggtcg atccgactc aagtcgagt cggaggagg 
      aggacgagg agacgtcca ccactgtgt ttagtaacg 
      tggaggtcg tccaagagg aggcgaaga aggaggagt 
      ctgatagta ataataaca ataacaagg aggagggta 
      acagttgcc aatattgta atatcgcgt tcggggacg 
      ccgtcctct acaccatcc atatgggct accacgggt 
      tccacaacc cgttcactt gcaacatgt gcggggtgg 
      aatgctccg ataaagtat cgttctttt tgcatattg 
      cacgcgttt ctcattcgt aaattaaga ctgattaga 
      gttaactag acttaggtt tgtgttttt tatttaact 
      agtcatacg gggccgcgg cccgatttc tcaatctag 
      ttattttac gcttttacg tgcaattta tttttgtta 
      accacaaat ggagatgta cgggttgga actccacag 
      ctgaaccaa atattttct ttcgccgaa ttaggtcaa 
      tttttaatt tatttttgt ctgtaacgt tttgtcgta 
      gtggtttta gtaaatagt cgagtttta ttgtcatat 
      ttttatact tgtggggcc atgacataa gactgatga 
      ttgtatttg taaataagt ttcaatgtt ttctatagc 
      tcgagcaac aatcaaaga ttattgtaa catagttgt 
      gtgtattat acctactat tgtttaagc caaataata 
      aacagtagc agagcaa
    • >TC013553 RB
      aatcgtgga gcttttgaa agcgcccga tggagggga 
      tgtctgtat tggaggggt gaagtcaag ccgtttggg 
      gtgggagga actggacat ggctatctg tgatttggc 
      atggtccga aacttattt ttcattcat gttcgagcg 
      gggacgtag tgtagtgtc tctcaaatt taatcattg 
      ttttttatc gagtgtagt atttttaaa aaactgttc 
      gagtgcatg tgaaatgat agataaaga catgaattc 
      ggcttgcat gaggggtgg ctcagtccg gactttaaa 
      caattacca acaagtgat ggaaccgcg gtcccccca 
      caccgcctg gcaattcgg cgtttccca gatcgtgaa 
      acgcgaacc aatggacga agataagaa cgattccgg 
      ggttacctc cggaagcga cttccattc atcctcccc 
      cagcagtga tacgagcca ggatttaca gcactctta 
      tcaatcgcc gcaaacgca accggcccg cttctactc 
      cactcccat cgtgcccca cttcgccta caaccacaa 
      ccccctaac accccccaa ctccgagcc actggtctc 
      ccccaagag cgagaagga ggagaagga catggagac 
      gaccctcac gccttgcgc gtccccgaa ccgcaagcc 
      ggacgacaa tcaggacca cctccgccg cctggagat 
      gtccctgga gaagagcgg cctgttctc gtccaaaac 
      gagcgaaca ctccgtgga tgagttgtc ggggaagag 
      cgacaacga cgccgagga gtacgacga gcagagctt 
      gcgcgtccc gaaagtcaa ctcgcacgg gaaaatcaa 
      aactttcaa atgcaaaca gtgcgattt tgtcgcaat 
      caccaaact cgaacagtg gaaccacag caaggtgca 
      catccggga ggacaagcg gctgacctg ccccaagtg 
      cccgttcat caccgagta caagcacca cctggagta 
      ccacctccg caaccacgc cgggtccaa accgttcca 
      atgcaacaa atgcgacta cacttgtgt gaacaagtc 
      catgctaaa ctcacacat gaagtcgca ctcgaacgt 
      ctaccgtta tagttgccg ggattgcag ctacgctac 
      caaatattg ccactcttt gaagatcca cctgaggcg 
      gtatggaca caccccgaa cgtggtttt ggacgagga 
      aggaaaccc gtgtccgga catcatcat cgacgtgca 
      tgggacgcg tcggggtcc caaaattaa gacacaacc 
      taaagccga agaggcgaa accggaaac gctcccgtt 
      ccttaacct gcaacaaca actaccttt ccctggtta 
      tcccttctt tggggggtt ccccaacgc ccagttgtt 
      gcaacaact gatccgcga gcgtcagtt ggcggccgg 
      gggttcaca ggaagagtc cagggttct ggatttgag 
      caaacctgg gtgtagtta caccgggga gcagaagag 
      tagacgcaa agggccggc gtttaaggt cgatccgac 
      tcaagtcga gtcggagga ggaggacga ggagacgtc 
      caccactgt gtttagtaa cgtggaggt cgtccaaga 
      ggaggcgaa gaaggagga gtctgatag taataataa 
      caataacaa ggaggaggg taacagttg ccaatattg 
      taatatcgc gttcgggga cgccgtcct ctacaccat 
      ccatatggg ctaccacgg gttccacaa cccgttcac 
      ttgcaacat gtgcggggt ggaatgctc cgataaagt 
      atcgttctt tttgcatat tgcacgcgt ttctcattc 
      gtaaattaa gactgatta gagttaact agacttagg 
      tttgtgttt tttatttaa ctagtcata cggggccgc 
      ggcccgatt tctcaatct agttatttt acgctttta 
      cgtgcaatt tatttttgt taaccacaa atggagatg 
      tacgggttg gaactccac agctgaacc aaatatttt 
      ctttcgccg aattaggtc aatttttaa tttattttt 
      gtctgtaac gttttgtcg tagtggttt tagtaaata 
      gtcgagttt tattgtcat atttttata cttgtgggg 
      ccatgacat aagactgat gattgtatt tgtaaataa 
      gtttcaatg ttttctata gctcgagca acaatcaaa 
      gattattgt aacatagtt gtgtgtatt atacctact 
      attgtttaa gccaaataa taaacagta gcagagcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY