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Gene information
General information
TC013551 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8130141..8131978
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013551
    iBeetle screen iB_05450

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013551 RA
      MPSTSSSLI DEFQRSWLT SLIGICILC LGIWLLTWN 
      EGRAVHHAH SLDEAYNRV ISLNPYENI RPELDGHLV 
      HITGPLGID EPLTEPEYG ISIQAVKLK RRVQMYQWV 
      EERSPRDYV DNGLDQNYE TSDYYYVTE WRDKVVDSS 
      SFYIRHGHH NPTEIPLKS HTYISPFVR VGHLMLGSE 
      IKEKFTDYV EVTSDERPE RKDVKLHMG IYYHCDDVW 
      NPEVGDIRV QFYYAGIAN EIVTVIAMQ KDGVLVPYT 
      TSKGHKIAL LRHGDLNIN QMFNAEHYD VRLETWKIR 
      AAGVFILYA SCVCLARLI KIFFFRIPF LRNIVAGDM 
      TSSTNFAIS ASVSLLVIA TAWILYRPV LGVGLVAAA 
      ISPFFYCTM GMYNIAQNQ NGFYNR
    CDS sequence
    • >TC013551 RA
      atgccctca acttcttcg tctctaata gacgagttc 
      cagaggagt tggttaact tctcttatc ggtatttgt 
      atactttgc ttaggaatt tggcttttg acatggaac 
      gagggcagg gcggtacac catgcccat tccttggat 
      gaggcatac aacagggtc atttcactc aacccttat 
      gagaatata aggccggag ttggacggc catttggtc 
      catatcacg ggcccttta gggattgat gaacctttg 
      actgaacct gaatatggt atttctatt caggctgtt 
      aagttgaaa cggagggtt caaatgtat cagtgggtt 
      gaagagcga tcacctagg gattacgtt gataacggc 
      ttagatcag aattacgaa acctccgat tattattat 
      gtcacagaa tggagagac aaggttgta gacagtagc 
      agtttttac ataagacat ggccaccac aaccccact 
      gaaatcccg ctaaaatca cacacctac atatccccc 
      tttgtccgc gttggccac ttgatgtta ggtagcgaa 
      attaaggaa aaattcact gattacgta gaggtcaca 
      agtgacgaa agacctgaa cgaaaagac gtgaaactc 
      cacatgggc atttactac cattgcgac gacgtttgg 
      aaccctgaa gtgggcgac atcagggtc caattttat 
      tacgctggg attgcaaac gaaatcgta actgtaatt 
      gcaatgcaa aaagacggc gttttggtc ccttacacg 
      accagcaaa ggccacaaa atcgcactt ttgcgacat 
      ggggaccta aatatcaat caaatgttc aatgctgaa 
      cactacgat gttagatta gagacgtgg aaaatcagg 
      gctgctggt gttttcatc ttgtacgcg tcctgtgtt 
      tgtttagcg agactgatt aaaattttc ttttttaga 
      attccattt ttacgaaat atagtggcc ggcgacatg 
      acctcttca acaaatttc gcgatctca gcttcagtc 
      tccttacta gtgattgcc acagcttgg attttgtac 
      cgacctgtg ttaggtgtg ggcctggtc gcggccgca 
      atcagccca ttcttctat tgcacaatg ggaatgtac 
      aacattgct caaaatcaa aacgggttc tacaataga 
      tga
    mRNA sequence
    • >TC013551 RA
      cataacccc aaatatgcc ctcaacttc ttcgtctct 
      aatagacga gttccagag gagttggtt aacttctct 
      tatcggtat ttgtatact ttgcttagg aatttggct 
      tttgacatg gaacgaggg cagggcggt acaccatgc 
      ccattcctt ggatgaggc atacaacag ggtcatttc 
      actcaaccc ttatgagaa tataaggcc ggagttgga 
      cggccattt ggtccatat cacgggccc tttagggat 
      tgatgaacc tttgactga acctgaata tggtatttc 
      tattcaggc tgttaagtt gaaacggag ggttcaaat 
      gtatcagtg ggttgaaga gcgatcacc tagggatta 
      cgttgataa cggcttaga tcagaatta cgaaacctc 
      cgattatta ttatgtcac agaatggag agacaaggt 
      tgtagacag tagcagttt ttacataag acatggcca 
      ccacaaccc cactgaaat cccgctaaa atcacacac 
      ctacatatc cccctttgt ccgcgttgg ccacttgat 
      gttaggtag cgaaattaa ggaaaaatt cactgatta 
      cgtagaggt cacaagtga cgaaagacc tgaacgaaa 
      agacgtgaa actccacat gggcattta ctaccattg 
      cgacgacgt ttggaaccc tgaagtggg cgacatcag 
      ggtccaatt ttattacgc tgggattgc aaacgaaat 
      cgtaactgt aattgcaat gcaaaaaga cggcgtttt 
      ggtccctta cacgaccag caaaggcca caaaatcgc 
      acttttgcg acatgggga cctaaatat caatcaaat 
      gttcaatgc tgaacacta cgatgttag attagagac 
      gtggaaaat cagggctgc tggtgtttt catcttgta 
      cgcgtcctg tgtttgttt agcgagact gattaaaat 
      tttcttttt tagaattcc atttttacg aaatatagt 
      ggccggcga catgacctc ttcaacaaa tttcgcgat 
      ctcagcttc agtctcctt actagtgat tgccacagc 
      ttggatttt gtaccgacc tgtgttagg tgtgggcct 
      ggtcgcggc cgcaatcag cccattctt ctattgcac 
      aatgggaat gtacaacat tgctcaaaa tcaaaacgg 
      gttctacaa tagatgaga cgattttgc aactcttag 
      cggttttct agtcagctg aaaaaatgt tcagcttag 
      gaggtgaaa acggagaaa tgctttcat tttgttgtt 
      gcaaccaca atagtgcga gttgtgatt tatttattg 
      tgattaaaa gattttata aagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY